Evaluation of serum cardiac biomarkers in sheep with acute lactic acidosis

PDF (English)
Pubblicato: Ιαν 29, 2018
DOI: https://doi.org/10.12681/jhvms.15608
M. FARTASHVAND
Department of Clinical Sciences, Faculty of Veterinary Medicine, Tabriz branch, Islamic Azad University
Y. HAJI-SADEGHI
Department of Clinical Sciences, Faculty of Veterinary Medicine, Tabriz branch, Islamic Azad University
Abstract

In this study we investigated the changes of cardiac injury biomarkers in serum samples from 200 sheep with acute ruminal lactic acidosis (ARLA) and 50 healthy controls belonging to the Ghezel breed. After clinical examination and recording of vital signs (heart rate, respiratory rate, rectal temperature and hydration status), rumen fluid and venous blood samples were collected. The pH of rumen fluid was determined using a paper tape pH-meter and lactic acid concentration of serum was measured using a commercially available method. Similarly, activities of AST, LDH, CK-MB and serum concentration of cTnI were measured using special commercial kits. According to the findings serum activities of AST (p = 0.007) and CK-MB (p = 0.002) in sheep with ARLA were significantly higher than in healthy animals. Serum LDH activity in the disease group was higher than in the control group, however this difference was statistically non-significant. cTnI concentration were 0.684 ± 0.03 ng/ml in sheep with ARLA, which was significantly higher than in healthy sheep (p = 0.000). There were significant negative correlations between ruminal pH and serum lactate levels, heart rate, respiratory rate and dehydration degree. The sheep with the lowest ruminal pH (3 cases with < 4.5) had the highest levels of cTnI (2.28 to 3.06 ng/mL), and all died. It can be concluded that lactic acidosis may cause some degree of heart damage, although further studies are needed to support this speculation.

Article Details
  • Sezione
  • Research Articles
Creative Commons License

Questo lavoro è fornito con la licenza Creative Commons Attribuzione - Non commerciale 4.0 Internazionale.

Authors who publish with this journal agree to the following terms:

· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Downloads
I dati di download non sono ancora disponibili.
Riferimenti bibliografici
Aberra A, Komukai K, Howarth FC, Orchard CH (2001) The effect of acidosis on the ECG of the rat heart. Exp Physiol 86:27–31.
Adin DB, Milner RJ, Berger KD, Engel C, Salute M (2005) Cardiac troponin I concentrations in normal dogs and cats using a bedside analyzer. J Vet Cardiol 7:27–32.
Babuin L, Jaffe AS (2005) Troponin: the biomarker of choice for the detection of cardiac injury. Can Med Assoc J 173:1191–1202.
Brown MS, Hallford DM, Galyean ML, Krehbiel CR, Duff G (1999) Effect of ruminal glucose infusion on dry matter intake, urinary nitrogen composition, and serum metabolite and hormone profiles in ewes. J Anim Sci 77:3068–3076.
Collinson PO, Gaze DC (2007) Biomarkers of cardiovascular damage and dysfunction– an overview. Heart, Lung and Circulation 16:S71–S82.
Constable PD (1999) Acute endotoxemia increases left ventricular contractility and diastolic stiffness in calves. Scock 12:391–401.
Coudrey L (1998) The troponins. Archive International Medicine 158:1173–1180.
Cunningham JG, Klein BG (2007) Textbook of Veterinary Physiology, 4th edn. St. Louis, Missouri, Saunders, Elsevier Publications. Dshurov A (1976) Pathomorfologic changes in the ruminal acidosis of sheep. Arch Exp Veterinärmed 30:881–887.
Fartashvand M, Nadalian MG, Sakha M, Safi S (2013) Elevated serum cardiac troponin I in cattle with theileriosis. J Vet Int Med 27:194–9.
Gunes V, Erdogan HM, Citil M, Ozcan K (2005) Assay of cardiac troponins in the diagnosis of myocardial degeneration due to footand-mouth disease in a calf. Vet Rec 156:714–715.
Gunes V, Ozcan K, Citil M, Onmaz AC, Erdogan HM (2010) Detection of myocardial degeneration with point-of-care cardiac troponin assays and histopathology in lambs with white muscle disease. Vet J 184:376–378.
Ilva TJ, Eskola MJ, Nikus KC, Voipio-Pulkki LM, Lund, J Pulkki K, Mustonen H, Niemelä KO, Karhunen PJ Porela P (2010) The etiology and prognostic significance of cardiac troponin I elevation in unselected emergency department patients. J Emerg Med 38:1–5.
Jafari-Dehkordi A, Haji-Hajikolaei MR, Karimi-Dehkordi Z (2011) ECG changes in acute experimental ruminal lactic acidosis in sheep. Vet Res Forum 2:203–208.
Kirbas A, Baydar E, Kandemir FM, Dorman E, Kizil O, Yildirim BA (2014) Evaluation of serum cardiac troponin I concentration in sheep with acute ruminal lactic acidosis. Veterinarski Arhiv, 84:355–364.
Leonardi F, Passeri B, Fusari A, De Razza P, Beghi C, Lorusso R, Corradi A, Botti P (2008) Cardiac troponin I (cTnI) concentration in an ovine model of myocardial ischemia. Res. Vet Sci 85:141–144.
O’Brien PJ, Landt Y, Ladenson JH (1997) Differential reactivity of cardiac and skeletal muscle from various species in a cardiac troponin I immunoassay. Clin Chem 43:2333–2338.
Onmaz AC, Gunes V, Aslan O, Bekdik IK (2011) Effects of experimentally induced ruminal lactic acidosis on electrocardiographic parameters in sheep. J Health Sci 20:210–216.
Orchard CH, Allen DG, Boyett MR, Elliott AC, Kirby MS (1993) Modulation of Cardiac Calcium Sensitivity. Oxford: Oxford University Press.
Orchard CH, Cingolani HE (1994) Acidosis and arrhythmias in cardiac muscle. Cardiovascular Res 28:1312–1319.
Orchard CH, Kentish JC (1990) Effects of changes on pH on the contractile function of cardiac muscle. Am J Physiol 258:C967–81.
Oyama MA, Solter PF (2004) Validation of an immunoassay for measurement of canine cardiac troponin-I. J Vet Cardiol 6:17–24.
Patra RC, Lal SB, Swamp D (1996) Biochemical profile of rumen liquor, blood and urine in experimental acidosis in sheep. Small Ruminant Res 19:177–180.
Peek SF, Apple FS, Murakami MA, Crump PM, Semrad SD (2008) Cardiac isoenzymes in healthy Holstein calves and calves with experimentally induced endotoxemia. Can J Vet Res 72:356–361.
Pelander L, Hagman R, Häggström J (2008) Concentrations of cardiac Troponin I before and after ovariohysterectomy in 46 female dogs with pyometra. Acta Veterinaria Scandinavica 50:35.
Pourjafar M, Mohammadnia AR, Jafari Dehkordi A (2004) The effects of experimentally induced lactic acidosis on serum glucose, BUN, serum electrolyte (K, Na, P, Ca), haematocrit, rumen pH, rumen microflora and pathological changes of ruminal epithelium in lori sheep. Pajouhesh-va-Sazandegi 17:27–36.
Radostits OM, Gay CC, Hinchcliff KW, Constable PD (2007) Veterinary Medicine, a text book of the disease of cattle, sheep, pigs and horses. 10th edn. Elsevier Publications.
Tunca R, Sozmen M, Erdogan H, Citil M, Uzlu E, Ozen H, Gokce E (2008) Determination of cardiac troponin I in the blood and heart of calves with foot-and-mouth disease. J Vet Diag Invest 20:598–605.
Varga A, Schober KE, Walker WL, Lakritz J, Michael D (2009) Validation of a commercially available immunoassay for the measurement of bovine cardiac troponin I. J Vet Int Med 23:359–365.
Wang AY, Lam CW, Wang M, Chan IH, Lui SF, Zhang Y, Sanderson JE (2009) Diagnostic potential of serum biomarkers for left ventricular abnormalities in chronic peritoneal dialysis patients. Nephrology Dialysis Transplantation 24:1962–1969.