Identification of new avian Infectious Bronchitis virus variants in Iranian poultry flocks by High Resolution Melting curve analysis

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DOI: https://doi.org/10.12681/jhvms.16425
A. MASOUDIAN
PhD Student of Poultry Diseases, Department of Poultry Diseases, Faculty of Veterinary Science, Science and Research Branch, Islamic Azad University
N. SHEIKHI
Department of Poultry Diseases, Faculty of Veterinary Science, Science and Research Branch, Islamic Azad University
M. H. BOZORGMEHRI-FARD
Department of Poultry Diseases, Faculty of Veterinary Science, Science and Research Branch, Islamic Azad University
Abstract

Avian Infectious bronchitis (IB) is a common coronavirus infection of chickens and responsible for performance reduction and increasing mortality due to subsequent respiratory, renal and/or reproductive disorders. Classification of causative agent is necessary to plan successful vaccination strategies to prevent the infection due to poor inter-strains cross-reaction. To identify dominant circulating strains in Iran, a Real-time PCR combined with 3’ Un-Translated Region (3’ UTR) High Resolution Melting (HRM) analysis designed as a rapid and reliable method for IB Virus (IBV) detection and differentiation. Samples collected from 20-suspected flocks and after PCR products, HRM curves of samples as well as 6 commercial IB live vaccines with 2 standard strains, were analyzed as references. IBV genomes detected in 11 samples while according to HRM analysis and calculating Genotype Confidence Percentage (GCP), 6 positive specimens identified as 793/B field strains and the left 5 found as new IBV variant strains. Then obtained PCR products sent for nucleotide sequencing to determine genotype relativity. All five infectious agents, related to QX-like type and indicating circulation of new variants in Iran as a probable cause of vaccination failures and consequent economical losses.

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