Honey bee drone (Apis mellifera) sperm cryopreservation with rainbow trout seminal plasma supplemented extenders

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Pubblicato: Apr 29, 2022
DOI: https://doi.org/10.12681/jhvms.25651
Selim Alçay
https://orcid.org/0000-0002-2472-8157
Sevinar Cakmak
https://orcid.org/0000-0001-6050-791X
Ibrahim Cakmak
https://orcid.org/0000-0002-8000-5770
Ahmet Aktar
https://orcid.org/0000-0002-2975-2594
Burcu Ustuner
https://orcid.org/0000-0001-5999-4685
Melih Yilmaz
https://orcid.org/0000-0001-6050-791X
Muhammed Duman
Hasan Ozkan
https://orcid.org/0000-0001-6664-1378
Yakup Akkaya
https://orcid.org/0000-0002-6443-193X
Hakan Sagirkaya
https://orcid.org/0000-0001-6619-3229
Zekariya Nur
https://orcid.org/0000-0002-1438-221X
Abstract

Bu çalışma, gökkuşağı alabalığı seminal plazmasının (RTSP) bal arısı drone spermatozoonu üzerindeki etkilerini eritme sonrası araştırmayı amaçladı. Cinsel açıdan olgun erkek arılardan sperm örnekleri toplandı ve havuzlandı. Daha sonra havuzlanmış semen, farklı konsantrasyonlarda RTSP (% 2.5 /% 5 /% 10) ile ve RTSP (kontrol) takviyeli sulandırıcılar olmadan seyreltildi. Motilite, plazma membranı fonksiyonel bütünlüğü, akrozom bütünlüğü ve mitokondriyal fonksiyon kriyoprezervasyon sürecinden olumsuz etkilenmiştir, ancak DNA bütünlüğü etkilenmemiştir. Membran lipid peroksidasyon durumu da çözüldükten sonra malondialdehit (MDA) konsantrasyonu kullanılarak analiz edildi. RTSP10 grupları, kontrol grubuna kıyasla sperm motilitesi, plazma membran bütünlüğü, akrozomal bütünlük ve mitokondriyal fonksiyon üzerinde olumlu bir etkiye sahipti (P <0.05). Çalışma, RTSP takviyeli sulandırıcıların eritme sonrası drone sperm parametreleri üzerinde faydalı etkilere sahip olduğunu göstermektedir. Bu çalışmanın sonuçları,% 10 RTSP takviyeli bir sulandırıcı kullanmanın avantajını göstermiştir.

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