Anthelmintic activity of Moringa oleifera and Azadirachta indica against gastrointestinal nematodes of wild sheep

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Pubblicato: Ιουλ 10, 2022
DOI: https://doi.org/10.12681/jhvms.25876
Azhar Rafique
M. Shahid Mahmood
Rao Zahid Abbas
Sultan Ali
Institute of Microbiology, University of Agriculture, Faisalabad, Pakistan
AH Mian
Department of Parasitology, UVAS, Lahore, Pakistan
A Rizwan
Institute of Microbiology, University of Agriculture, Faisalabad, Pakistan
Abstract

Gastrointestinal nematodes (GINs) are serious issue for health of wild sheep kept in captivity. Chemically synthesized anthelmintics are regularly used to control these parasites. In recent years anthelmintic resistance and remnant of drugs in animal products leads to use of medicinal plants as alternative to anthelmintics. In current study, the efficacy of aqueous, methanolic and ethanolic dried leaf extracts of medicinal plants Moringa oleifera and Azadirachta indica were tested for in vitro ovicidal and larvicidal activities against Haemonchus, Trichuris, Coccidia and Trichostrongylus; naturally acquired nematodes isolated from wild sheep (Ovis orientalis orientalis). Six concentrations of these plants extract (1.56, 3.13, 6.25, 12.5, 25 and 50 mg/ml) were evaluated using egg hatching assay (EHA) and larval development assay (LDA) in three replicates. To compare treatment effects, untreated and treated (0.1% ivermectin) controls were used. The aqueous, methanolic and ethanolic leaf extracts showed anthelmintic activities against isolated genera of nematodes but the inhibition was  maximum (99%) in ethanol extract of M. oleifera followed by methanol extract (97%) at maximum concentration tested at (50mg/ml). The overall findings of this study shows that Moringa oleifera and Azadirachta indica leaf extracts possess significant anthelmintic efficacy against GINs of sheep and these could be a natural alternative to synthetic anthelmintics to treat the worm infections in animals.

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Riferimenti bibliografici
Abbas RZ, Zaman MA, Sindhu ZUD, Sharif M, et al. (2020). Anthelmintic
effects and toxicity analysis of herbal dewormer against the
infection of Haemonchus contortus and Fasciola hepatica in goat.
Pakistan Veterinary Journal 40:455-460.
Abdel-Shafy S, Zayed AA (2002). In-vitro acaricidal effect of plant extract
of neem seed oil (Azadirachta indica) on egg, immature, and
adult stages of Hyalomma anatolicum excavatum (Ixodoidea: Ixodidae).
Veterinary Parasitology 106:89-96.
Abou Laila M, Abd El-Aziz AR, Menshawy S, Yokoyama N et al. (2021).
Evaluation of the inhibitory effects of coumermycin A1 on the growth
of Theileria and Babesia parasites in-vitro and in-vivo. Pakistan Veterinary
Journal 41:469-474.
Ademola IO, Eloff JN (2010). In-vitro anthelmintic activity of Combretum
molle (R. Br. ex G. Don) (Combretaceae) against Haemonchus contortus
ova and larvae. Veterinary Parasitology 169:198-203.
Adoho ACC, Konmy BBS, Olounladé PA, Azando EVB, Hounzangbé-
Adoté MS, et al. (2022). Phytochemistry and larval toxicity of
Ipomea asarifolia, Commelina diffusa, Acalypha ciliata and Eleusine
indica against Artemia salina. International Journal of Veterinary Science
:121-128.
Akhtar MS, Iqbal Z, Khan MN, Lateef M (2000). Anthelmintic activity
of medicinal plants with particular reference to their use in animals
in the Indo-Pakistan subcontinent. Small Ruminant Research 38:99-
Alawa CBI, Adamu AM, Gefu JO, Ajanusi OJ, Abdu PA, et al (2003).
In-vitro screening of two Nigerian medicinal plants (Vernonia amygdalina
and Annona senegalensis) for anthelmintic activity. Veterinary
Parasitology 113:73-81.
Assis LM, Bevilaqua CML, Morais SM, Viera LS, Costa CTC, Souza
JAI (2003). Ovicidal and larvicidal activity in-vitro of Spegelia anthlmialinn.
Extracts on Haemonchus contortus. Veterinary parasitology
:43-49.
Athanasiadou S, Kyrazakis I, Jakson F, Coop RL (2001). Direct anthelminthic
effects of condensed tannins towards different gastrointestinal
nematodes of sheep: in-vitro and in-vivo studies. Veterinary Parasitology
:205-219.
Azam F, Munier S, Batool M, Ahmad B, Abbas G (2019). A review on
advancements in ethnomedicine and phytochemistry of Tribulus terrestris;
a plant with multiple health benefits. International Journal of
Biosciences 14: 21-37.
Baz MM, Hegazy MM, Khater HF, El-Sayed YA (2021). Comparative
evaluation of five oilresin plant extracts against the mosquito larvae,
Culex pipiens Say (Diptera: culicidae). Pakistan Veterinary Journal
:191-196.
Cavalcante M, Ferreira PV, Paixão SL, Costa JC, Pereira RG, Madalena
JAS (2009). Potenciais produtivo e genético de clones de batata-doce.
Acta Scientiarum Agronomy 31:421-426.
Ceylan O, Uslu A, OztürkO,Sevinc F (2021). Serological investigation of
some vectorborne parasitic and rickettsial agents in dogs in the western
part of Turkey. Pakistan Veterinary Journal 41:386-392.
Chinchilla-Carmona M, Valerio-Campos I, Gutiérrez-Espeleta GA, Soto-
Fournier S, Vanegas-Pissa JC, et al. (2020). Intestinal parasites
found in faecal samples of wild cats of Costa Rica. International
Journal of Veterinary Science 9:153-156.
Costa CTC, Bevilaqua CML, Camurça-Vasconcelos ALF, Maciel MV,
Morais SM et al (2008). In vitro ovicidal and larvicidal activity of
Azadirachta indica extracts on Haemonchus contortus. Small Ruminant
Research 74:284-287.
Debella A (2002). Preliminary screening techniques of secondary metabolites.
Manual for Phytochemical Screening of Medicinal Plants,
pp:38-57.
Degla LH, Kuiseu J, Olounlade PA, Attindehou S, Hounzangbe-Adote
MS, et al. (2022). Use of medicinal plants as alternative for the control
of intestinal parasitosis: assessment and perspectives. Agrobiological
Records 7:1-9.
Doligalska M, Jóźwicka K, Kiersnowska M, Mroczek A, Pączkowski C,
Janiszowska W (2011). Triterpenoid saponins affect the function of
P-glycoprotein and reduce the survival of the free-living stages of
Heligmosomoides bakeri. Veterinary Parasitology 179:144-151.
Eguale T, Tadesse D, Giday M (2011). In vitro anthelmintic activity of
crude extracts of five medicinal plants against egg-hatching and larval
development of Haemonchus contortus. Journal of Ethnopharmacology
:108-113.
Eguale T, Tilahun G, Debella A, Feleke A, Makkonen E (2007). Haemonchus
contortus: In-vitro and in-vivo anthelmintic activity of aqueous
and hydroalcoholic extracts of Hedera helix. Experimental Parasitology
:340-345.
Finney DJ (1971). Probit analysis, Cambridge University Press. Cambridge,
UK.
Hammond JA, Fielding D, Bishop SC (1997). Prospects for plant anthelmintics
in tropical veterinary medicine. Veterinary Research Communications
:213-28.
Hassan NMF, Sedky D, Abd El-Aziz TH, Shalaby HA, Abou-Zeina HAA
(2020). Anthelmintic potency and curative effect of pomegranate
peels ethanolic extract against H. contortus infection in goats. International
Journal of Veterinary Science 9:210-216.
Hoberg EP, Polley L, Jenkins EJ, Kutz SJ, Veitch AM, Elkin BT (2008).
Integrated approaches and empirical models for investigation of parasitic
diseases in northern wildlife. Emerging Infectious Diseases
:10-17.
Hoste H, Jackson F, Athanasiadou S, Thamsborg SM, Hoskin S (2006).
The effects of tannin rich plants on parasitic nematodes in ruminants.
Trends in Parasitology 22:253-261.
Hounzangbe-Adote S, Fouraste I, Moutairou K, Hoste H (2005). In-vitro
effects of four tropical plants on three life-cycle stages of the parasitic
nematode Haemonchus contortus. Research in Veterinary Science
:155-160.
Iqbal Z, Sarwar M, Jabbar A, Ahmed S, Nisa M, Sajid MS, Khan MN,
Mufti KA, Yaseen M (2007). Direct and indirect anthelmintic effects
of condensed tannins in sheep. Veterinary Parasitology, 144:125-131.
Jabbar A, Iqbal Z, Khan MN (2006). In-vitro anthelmintic activity of Trachyspermum
ammi seeds. Pharmacognosy Magazine 2:126-129.
Jayaprakasha GK, Singh RP, Sakariah KK (2001). Antioxidant activity
of grape seed (Vitisvinifera) extracts on peroxidation models in-vitro.
Food Chemistry 73:285-290.
Majeed Y, Shaukat MB, Abbasi KY, Ahmad MA (2021). Indigenous
plants of Pakistan for the treatment of diabetes: A review. Agrobiological
Records 4:44-63.
Marie-Magdeleine C, Hoste H, Mahieu M, Varo H, Archimede H (2009).
In-vitro effects of Curcurbita moschata seed extracts on. Haemonchus
contortus. Veterinary Parasitology 161:99-105.
Melo FMPD, Fiore MF, Moraes LABD, Silva-Stenico ME, Scramin S,
Teixeira MDA, Melo ISD (2009). Antifungal compound produced by
the cassava endophyte Bacillus pumilus MAIIIM4A. Scientia Agricola
:583-592.
Min BRM, Hart SP (2003). Tannins for suppression of internal parasites.
Journal of Animal Science 81 (E. Suppl. 2):102-109.
Mishra V, Parveen N, Singhal KC, Khan NU (2005). Antifilarial activity
of Azadirachta indica on cattle filarial parasite Setaria cervi. Fitoterapia
:54-61.
Nguyen PH, Hasek J, Kohlwein SD, Romero C, Choi JH, Vancura A
(2005). Interaction of Pik1p and Sjl proteins in membrane trafficking.
FEMS Yeast Research 5:363-71.
Pan SY, Zhou SF, Gao SH, Yu ZL, Zhang SF et al (2013). New perspectives
on how to discover drugs from herbal medicines: CAM’s outstanding
contribution to modern therapeutics. Evidence-Based Complementary
and Alternative Medicine 1-25.
Pessoa LM, Morias SM, Bevilaqua CM, Luciano JH (2002). Anthelmintic
activity of essential oil of Ocimum gratissimum Linn. andeuginol
against Haemonchus contortus. Veterinary Parasitology 109:59-63.
Rafay M, Ghaffar MU, Abid M, Malik Z, Madnee M (2021). Phytochemicals
Analysis and antimicrobial activities of Echinops echinatus from
Cholistan Desert, Pakistan. Agrobiological Records 5:21-27.
Ragab E, H Badr, AAbuElkheir, MI Enbaawy (2020). The effectiveness
of methanolic extracts of five plants on different Salmonella isolates.
International Journal of Veterinary Science 9:379-384.
Rastogi T, Bhutda V, Moon K, Aswar PB, Khadabadi SS (2009). Comparative
studies on anthelmintic activity of Moringa oleifera and Vitex
negundo. Asian Journal of Research in Chemicals 2:181-182.
Raza QS, Saleemi MK, Gul ST, Irshad H, Fayyaz A, et al. (2022). Role of
essential oils/volatile oils in poultry production - A review on present,
past and future contemplations. Agrobiological Records 7:40-56.
Singh V, Varshney P, Dash SK and Lal HP (2013). Prevalence of gastrointestinal
parasites in sheep and goats in and around Mathura, India,
Veterinary World 6:260-262.
Sobhy H, AboElnaga TR, Behour TS, Razin EA (2021). In vitro trypanocidal
activity of essential oils of some plants against Trypanosoma
evansi. International Journal of Veterinary Science 10:191-195.
Štrbac F, Bosco A, Amadesi A, Rinaldi Let al. (2021). Ovicidal potential
of five different essential oils to control gastrointestinal nematodes of
sheep. Pakistan Veterinary Journal 41:353-358.
Taylor MA, Learmount J, Lunn E, Morgan C, Craig BH (2009). Multiple
resistances to anthelmintics in sheep nematodes and comparison of
methods used for their detection. Small Ruminant Research 86:67-70.
Vieira RFC, BarbosaTS, Morinishi CK, Ciarlini PC, Bomfim SRM
(2007). Volume globular de cães determinado por impedância e método
de microhematócrito (strumia). Acta Scientiae Veterinariae Porto
Alegre 35:433-435.