Bacteriospermia assessment and its relationship with conventional seminal parameters in stud dogs ejaculates (Canis familiaris)

PDF (English)
Pubblicato: Jan 20, 2023
DOI: https://doi.org/10.12681/jhvms.27558
S. Agudelo-Yepes
Grupo Reproducción, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad de Antioquia -UdeA, Medellín, Colombia
J. Puerta-Suárez
Grupo Reproducción, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad de Antioquia -UdeA, Medellín, Colombia
D.F. Carrillo-Gonzalez
One Health and Veterinary Innovative Research & Development Group, Escuela de Medicina Veterinaria, Universidad de Antioquia - UdeA, Medellín, Colombia
Walter D Cardona Maya
https://orcid.org/0000-0003-0062-6444
Abstract

The objective of this work was to determine the frequency of bacteriospermia and its effect on the seminal quality in canines. Dogs were divided into two groups according to weight: small dogs between 1 and 10 kg (Group 1) and dogs with more than 10 kg (Group 2). The foreskin was disinfected in each animal (n=15), and the semen sample was collected by the gloved hand method. Sperm motility, morphology, viability, and concentration were evaluated using a 40x microscope. A 10µL of semen drop was cultured by diffusion method on blood agar and MacConkey agar. Colony-forming units (CFU) were quantitatively evaluated, and biochemical identification was carried out after 48 hours at 37°C. Conventional PCR was performed on the semen samples to evaluate the presence/absence of 11 bacteria. Bacterial growth was found in all samples. The CFU/mL in blood agar were 34042.8 for group 1 and 107714.3 for group 2, while on MacConkey agar were 142.9 CFU/mL, and 21328.6 CFU/mL, respectively. Coagulase-negative Staphylococcus was the most frequent bacteria isolated by conventional culture (64.3%), and Staphylococcus aureus and Klebsiella spp. were the most common bacteria found by conventional PCR. Between both groups, only a statistically significant difference was found in normal sperm morphology. A negative correlation was observed between viability and morphology with CFU on MacConkey agar. One dog was ruled out because he had azoospermia. Canine seminal bacteriospermia is quite frequent and could alter its quality. The presence of Gram-negative bacteria is associated with greater alteration in the semen analysis.

Article Details
  • Sezione
  • Research Articles
Creative Commons License

Questo lavoro è fornito con la licenza Creative Commons Attribuzione - Non commerciale 4.0 Internazionale.

Authors who publish with this journal agree to the following terms:

· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Downloads
I dati di download non sono ancora disponibili.
Biografia autore
D.F. Carrillo-Gonzalez, One Health and Veterinary Innovative Research & Development Group, Escuela de Medicina Veterinaria, Universidad de Antioquia - UdeA, Medellín, Colombia

Facultad de Ciencias Agropecuarias, Escuela de Zootecnia, Universidad de Sucre, Sincelejo, Colombia

Riferimenti bibliografici
Agudelo-Yepes, S., Militza Rivillas, Y., Carrillo-González, D.F., Cardona-Maya, W.D., 2020. Clinical, ultrasound and chromosome findings of testicles in a French bulldog (Canis lupus familiaris) with azoospermia. Vet. Stanica 51, 317–324. https://doi.org/10.46419/vs.51.3.8
Angrimani, D.S.R., Lucio, C.F., Veiga, G.A.L., Silva, L.C.G., Regazzi, F.M., Nichi, M., Vannucchi, C.I., 2014. Sperm maturation in dogs: sperm profile and enzymatic antioxidant status in ejaculated and epididymal spermatozoa. Andrologia 46, 814–819. https://doi.org/10.1111/and.12154
Betancur, G.R., Araque, N.V., Garcia, E.A., 2009. Criopreservación de semen canino y su aplicación en la inseminación artificial. Rev. CES 4, 119–29.
Bjurström, L., Linde-Forsberg, C., 1992. Long-term study of aerobic bacteria of the genital tract in stud dogs. Am. J. Vet. Res. 53, 670–73.
Cano-Cháves, A., Galarzo-Pardo, S., Puerta-Suárez, J., Giraldo, M., Cadavid, Á.P., Cardona-Maya, W.D., 2017. Efecto de las bacterias uropatógenas y de los factores solubles de su metabolismo sobre la calidad espermática: Escherichia coli y Enterococcus faecalis. Clin Invest Ginecol Obstet 44, 106–112. https://doi.org/10.1016/j.gine.2015.11.005
Chłopik, A., Wysokińska, A., 2020. Canine spermatozoa. What do we know about their morphology and physiology? An overview. Reprod. Domest. Anim. 55, 113–126. https://doi.org/10.1111/rda.13596
Diemer, T., Weidner, W., Michelmann, H.W., Schiefer, H.G., Rovan, E., Mayer, F., 1996. Influence of Escherichia coli on motility parameters of human spermatozoa in vitro. Int. J. Androl. 19, 271–277. https://doi.org/10.1111/j.1365-2605.1996.tb00475.x
Dragonetti, A., Solis, C., Giordano, A., 2005. Prostatitis en el perro. Analecta Vet. 25, 33–39.
Eilts, B.E., 2005. Theoretical aspects of canine cryopreserved semen evaluation. Theriogenology 64, 685–91. https://doi.org/10.1016/j.theriogenology.2005.05.017
Esmailkhani, A., Akhi, M.T., 2018. Assessing the prevalence of Staphylococcus aureus in infertile male patients in Tabriz, northwest Iran. Int J Reprod BioMed 16, 469–74.
Freshman, J.L., 2002. Semen collection and evaluation. Clin. Tech. Small Anim. Pract. 17, 104–107. https://doi.org/10.1053/svms.2002.34326
Galarzo Pardo, S., Cano Cháves, M.A., Puerta Suarez, J., Giraldo, M., Mayorga, B.J.M., Cadavid, Á.P., Cardona Maya, W., 2015. Efecto de los factores solubles de Staphylococcus aureus, Staphylococcus capitis y Staphylococcus epidermidis sobre la funcionalidad espermática. Rev. Chil. Obstet. Ginecol. 80, 316–323. https://doi.org/10.4067/S0717-75262015000400006
Goericke-Pesch, S., Weiss, R., Hoffmann, B., 2006. Bacterial flora in dog semen. Hund/Katze 34, 17–21.
Goericke-Pesch, S., Weiss, R., Wehrend, A., 2011. Bacteriological findings in different fractions of canine ejaculates showing normospermia, teratozoospermia or azoospermia. Aust. Vet. J. 89, 318–322. https://doi.org/10.1111/j.1751-0813.2011.00808.x
Gradil, C.M., Yeager, A., Concannon, P.W., 2006. Evaluación de los problemas reproductivos del macho canino. Recent Adv. Small Anim. Reprod. 3, 20–40.
Guerrero-Hurtado, L.S., Puerta-Suárez, J., Cardona-Maya, W.D., 2018. Papel de los espermatozoides en la transmisión de bacterias uropatógenas: Escherichia coli y Enterococcus faecalis. Clin Invest Ginecol Obstet 45, 2–6. https://doi.org/10.1016/j.gine.2016.03.003
Gupta, S., Prabha, V., 2012. Human Sperm Interaction with Staphylococcus aureus: A Molecular Approach. J. Pathog. 2012, 1–7. https://doi.org/10.1155/2012/816536
Hesser, A., Darr, C., Gonzales, K., Power, H., Scanlan, T., Thompson, J., Love, C., Christensen, B., Meyers, S., 2017. Semen evaluation and fertility assessment in a purebred dog breeding facility. Theriogenology 87, 115–23. https://doi.org/10.1016/j.theriogenology.2016.08.012
Kolster, K.A., 2018. Evaluation of Canine Sperm and Management of Semen Disorders. Vet. Clin. North Am. Small Anim. Pract. 48, 533–545. https://doi.org/10.1016/j.cvsm.2018.02.003
Lopate, C., 2012. The Problem Stud Dog. Vet. Clin. North Am. Small Anim. Pract. 42, 469–88. https://doi.org/10.1016/j.cvsm.2012.01.014
Mar, A., Sharma, R., 2010. Assessment of sperm factors possibly involved in early recurrent pregnancy loss. Am. Soc. Reprod. Med. 94, 1465–72.
Memon, M.A., 2007. Common causes of male dog infertility. Theriogenology 68, 322–328. https://doi.org/10.1016/j.theriogenology.2007.04.025
Morales M., P., Contardo O., F., Cifuentes R., F., Rojas Z., C., 2011. Análisis de la fertilidad potencial del canino (Canis Lupus Familiaris) posterior a la inyección intratesticular de Cloruro de Calcio al 20%. Av. En Cienc. Vet. 26, 27–32.
Moreno, N., Agudelo-Flórez, P., 2010. Aplicación de la pruebas de PCR convencional simple y múltiple para la identificación de aislamientos de Leptospira spp. en Colombia. Rev Peru Med Exp Salud Publica 27, 548–56.
Moretti, E., Capitani, S., Figura, N., Pammolli, A., Federico, M.G., Giannerini, V., Collodel, G., 2009. The presence of bacteria species in semen and sperm quality. J. Assist. Reprod. Genet. 26, 47–56. https://doi.org/10.1007/s10815-008-9283-5
Olsen, H.B., 2020. Genetic correlations between body weight, daily weight gain, and semen characteristic traits in young Norwegian Red bulls. Am. Dairy Sci. Assoc. 103, 6311–17.
Pesch, S., Weiss, R., Hoffmann, B., 2006. Zur bakteriellen Flora im Rüdenejakulat. Tierärztl. Prax. Ausg. K Kleintiere Heimtiere 34, 17–21. https://doi.org/10.1055/s-0037-1622504
Puerta Suárez, J., Cardona Maya, W.D., 2016. Evaluación in vitro del efecto de Neisseria gonorrhoeae y los factores solubles producto de su metabolismo sobre la calidad espermática. Rev. Chil. Obstet. Ginecol. 81, 211–217. https://doi.org/10.4067/S0717-75262016000300007
Puerta Suárez, J., Villegas Castaño, A., Serna Quintana, G.J., Martínez, A., Romero Palacio, J., Giraldo, M., Cadavid, Á., Cardona Maya, W., 2015. Espermocultivo: crecimiento bacteriano del eyaculado y su relación con los parámetros seminales. Rev. Chil. Obstet. Ginecol. 80, 33–40. https://doi.org/10.4067/S0717-75262015000100005
Rijsselaere, T., Soom, A., Tanghe, S., Coryn, M., Maes, D., Kruif, A., 2005. New techniques for the assessment of canine semen quality: A review. Theriogenology 64, 706–19. https://doi.org/10.1016/j.theriogenology.2005.05.021
Ríos Sánchez, E., Calleros, E., González Zamora, A., Rubio, J., Martínez, O.C., Martínez, A., Hernández, S., Pérez Morales, R., 2016. Comparative analysis of different DNA extraction methods and their genotyping efficiency in Mexican population. Acta Univ. 26, 56–65. https://doi.org/10.15174/au.2016.1078
Rodríguez, E., Gil-Villa, A.M., Aguirre-Acevedo, D.C., Cardona-Maya, W., Cadavid, Á.P., 2011. Evaluación de parámetros seminales no convencionales en individuos cuyas parejas presentan muerte embrionaria temprana recurrente: en busca de un valor de referencia. Biomédica 31, 100–7. https://doi.org/10.7705/biomedica.v31i1.340
Root Kustritz, M.V., 2007. The value of canine semen evaluation for practitioners. Theriogenology 68, 329–37. https://doi.org/10.1016/j.theriogenology.2007.04.017
Salt, C., Morris, P.J., Butterwick, R.F., Lund, E.M., Cole, T.J., German, A.J., 2020. Comparison of growth patterns in healthy dogs and dogs in abnormal body condition using growth standards. PLOS ONE 15, 1–20. https://doi.org/10.1371/journal.pone.0238521
Zuleta-González, M.C., Zapata-Salazar, M.E., Guerrero-Hurtado, L.S., Puerta-Suárez, J., Cardona-Maya, W.D., 2019. Klebsiella Pneumoniae and Streptococcus Agalactie: Passengers in the sperm travel. Arch Esp Urol 72, 939–47.