Elisa And Mat Tests For Leptospira Interogans Detection From Cattle In Eastern Algeria
Abstract
Leptospira interrogans is one of the most common infectious organisms worldwide that causes several
losses in cattle. As far as we know, few previous studies have focused on Leptospira interrogans infection in cattle
from Algeria. This study aimed to assess the prevalence of bovine leptospirosis, identify selected risk factors, and
compare two different detection tests. 611 blood samples from 67 cattle farms were collected in six Algerian provinces;
Algiers, Boumerdès, Bordj Bou Arreridj, Sétif, Batna, and Souk Ahres. Sera samples were analyzed for the presence of
antibodies against five serovars of Leptospira interrogans using a microscopic agglutination test (MAT), using 50% agglutination,
at a dilution ≥1: 100 as a cut-off point. A commercial indirect enzyme-linked immunosorbent assay (ELISA)
test was used to determine the seroprevalence against L. interrogans serovar Hardjo. Moreover, a survey through
breeders’ questionnaires was conducted to identify the potential risk factors of Leptospira interrogans infection. The
seroprevalence of L. interrogans infection using MAT in the cows was 17.02% (95% confidence interval [CI]: 14.12-
20.24) and in the farms was 83.58% (95% CI: 59.31-81.99). The most commonly detected serovar was Hardjo 6.71%
(95% CI: 4.86-8.99) followed by Icterohaemorrhagiae 5.07% (95% CI: 3.47-7.12). Finally, the last serovar present was
the Grippotyphosa 2.78 (95% CI: 1.63-4.42). The comparison between the two serological methods, considering the
MAT as the reference test, shows that the PrioCHeck ELISA kit had a sensitivity of 63.4% (95% CI: 48.7-78.2), a specificity
of 98.9% (95% CI: 98.1-99.8), and a reliability of 96.6% (95% CI: 95.1-98.0). The kappa coefficient was 0.62,
and the McNemar test showed a P = 0.23. Multivariable logistic regression analysis showed that the semi-intensive
system was a protective factor against leptospirosis, with an odds ratio of 0.35 (95% CI: 0.16-0.78). The study findings
indicate that leptospirosis is a serious issue in farms located in selected provinces in Algeria, with a high incidence rate
noted there. The semi-intensive system’s significance as a leptospirosis protective factor is to create control strategies
that decrease the probability of infection in both humans and cattle.
Article Details
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Benammar, L., Benseghir, H., Hezil, D., Bouchemla, F., Zaidi, S., Bessas, A., & Ghalmi, F. (2025). Elisa And Mat Tests For Leptospira Interogans Detection From Cattle In Eastern Algeria. Journal of the Hellenic Veterinary Medical Society, 76(1), 8905–8916. https://doi.org/10.12681/jhvms.38367
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Riferimenti bibliografici
Aliberti, A., Blanda, V., Di Marco Lo Presti, V., Macaluso, G., Galluzzo,
P., Bertasio, C., Sciacca, C., Arcuri, F., D’Agostino, R., Ippolito, D.,
Pruiti Ciarello, F., Torina, A., Grippi, F., 2022. Leptospira interrogans
Serogroup Pomona in a Dairy Cattle Farm in a Multi-Host Zootechnical
System. Vet. Sci. 9. https://doi.org/10.3390/vetsci9020083
Alonso-Andicoberry, C., García-Peña, F.J., Pereira-Bueno, J., Costas,
E., Ortega-Mora, L.M., 2001. Herd-level risk factors associated
with Leptospira spp. seroprevalence in dairy and beef cattle in
Spain. Prev. Vet. Med. 52, 109-117. https://doi.org/10.1016/s0167-
(01)00249-5
André-Fontaine, G., 2016. Leptospirosis in domestic animals in France:
serological results from 1988 to 2007. Rev. Sci. Tech. 35, 913-923.
Bahaman, A.R., Ibrahim, A.L., Adam, H., 1987. Serological prevalence of
leptospiral infection in domestic animals in West Malaysia. Epidemiol.
Infect. 99, 379-392. https://doi.org/10.1017/s0950268800067868
Balamurugan, V., Alamuri, A., Bharathkumar, K., Patil, S.S., Govindaraj,
G.N., Nagalingam, M., Krishnamoorthy, P., Rahman, H., Shome,
B.R., 2018. Prevalence of Leptospira serogroup-specific antibodies in
cattle associated with reproductive problems in endemic states of India.
Trop. Anim. Health Prod. 50, 1131-1138. https://doi.org/10.1007/
s11250-018-1540-8
Benkirane, A., Noury, S., Hartskeerl, R.A., Goris, M.G.A., Ahmed, A.,
Nally, J.E., 2016. Preliminary Investigations on the Distribution of
Leptospira Serovars in Domestic Animals in North-west Morocco.
Transbound. Emerg. Dis. 63, e178-84. https://doi.org/10.1111/
tbed.12252
Benseghir, H., 2021. Seroepidemiolodical study of Leptospira interrogans
infection of cattle in North Algeria. Agricultura 1, 117-118. https://
doi.org/10.15835/agrisp.v118i1-2.14069
Benseghir, H., Amara-Korba, Azzag, N., Hezil, Ghalmi, F., 2020. Seroprevalence
and risk factors for leptospirosis in cattle Original Article
Open Access Seroprevalence of and associated risk factors for Leptospira
interrogans serovar Hardjo infection of cattle in Setif, Algeria.
African J. Clin. Exp. Microbiol. 21, 185-191.
Bertelloni, F., Cilia, G., Turchi, B., Pinzauti, P., Cerri, D., Fratini, F., 2019.
Epidemiology of leptospirosis in North-Central Italy: Fifteen years
of serological data (2002-2016). Comp. Immunol. Microbiol. Infect.
Dis. 65, 14-22. https://doi.org/10.1016/j.cimid.2019.04.001
Biggs, H.M., Bui, D.M., Galloway, R.L., Stoddard, R.A., Shadomy, S.
V, Morrissey, A.B., Bartlett, J.A., Onyango, J.J., Maro, V.P., Kinabo,
G.D., Saganda, W., Crump, J.A., 2011. Leptospirosis among hospitalized
febrile patients in northern Tanzania. Am. J. Trop. Med. Hyg. 85,
Bomfim, M.R.Q., Ko, A., Koury, M.C., 2005. Evaluation of the recombinant
LipL32 in enzyme-linked immunosorbent assay for the serodiagnosis
of bovine leptospirosis. Vet. Microbiol. 109, 89-94. https://doi.
org/10.1016/j.vetmic.2005.05.002
Cannon, R. M. & Roe, R.T., 1982. Livestock Diseases Surveys. A Field
Manual for Veterinarians. Canberra: Australian Bureau of Animal
Health.
Cerri, D., Ebani, V. V, Fratini, F., Pinzauti, P., Andreani, E., 2003. Epidemiology
of leptospirosis: observations on serological data obtained
by a “diagnostic laboratory for leptospirosis” from 1995 to 2001.
New Microbiol. 26, 383-389.
Cilia, G., Bertelloni, F., Mignone, W., Spina, S., Berio, E., Razzuoli, E.,
Vencia, W., Franco, V., Cecchi, F., Bogi, S., Turchi, B., Cerri, D., Fratini,
F., 2020. Molecular detection of Leptospira spp. in wild boar (Sus
scrofa) hunted in Liguria region (Italy). Comp. Immunol. Microbiol.
Infect. Dis. 68, 101410. https://doi.org/10.1016/j.cimid.2019.101410
Coppola, F., Cilia, G., Bertelloni, F., Casini, L., D’Addio, E., Fratini, F.,
Cerri, D., Felicioli, A., 2020. Crested Porcupine (Hystrix cristata L.):
A New Potential Host for Pathogenic Leptospira Among Semi-Fossorial
Mammals. Comp. Immunol. Microbiol. Infect. Dis. 70, 101472.
Deneke, Y., Sabarinath, T., Gogia, N., Lalsiamthara, J., Viswas, K.N.,
Chaudhuri, P., 2014. Evaluation of recombinant LigB antigen-based
indirect ELISA and latex agglutination test for the serodiagnosis of
bovine leptospirosis in India. Mol. Cell. Probes 28, 141-146. https://
doi.org/10.1016/j.mcp.2014.01.001
Derdour, S.-Y., Hafsi, F., Azzag, N., Tennah, S., Laamari, A., China, B.,
Ghalmi, F., 2017. Prevalence of The Main Infectious Causes of Abortion
in Dairy Cattle in Algeria. J. Vet. Res. 61, 337-343. https://doi.
org/10.1515/jvetres-2017-0044
Dreyfus, A., Wilson, P., Benschop, J., Collins-Emerson, J., Verdugo, C.,
Heuer, C., 2018. Seroprevalence and herd-level risk factors for seroprevalence
of Leptospira spp. in sheep, beef cattle and deer in New
Zealand. N. Z. Vet. J. 66, 302-311. https://doi.org/10.1080/00480169
.2018.1507770
Egan, J., 1986. Leptospira interrogans serovar hardjo in the Republic of
Ireland, Martinus N. ed. The Netherlands.
El-Sukhon, S.N., Abo-Shehada, M.N., Abuharfiel, N., Atmeh, R.F.,
Prevalence of leptospiral antibodies in cattle in northern Jordan.
Trop. Anim. Health Prod. 24, 127-128. https://doi.org/10.1007/
BF02356959
El Jalii, I.M., 2008. Comparison between ELISA and the microscopic agglutination
test for the diagnosis of bovine leptospirosis 61, 73-75.
Ellis, W.A. & Thiermann, A.B., 1986. Isolation of leptospires from the
genital tracts of Iowa. Am. J. Vet. Res. 1694-1696.
Espi, A., Prieto, J.M., Fernandez, M., Alvarez, M., 2000. Serological
prevalence to six leptospiral serovars in cattle in Asturias (Northern
Spain). Epidemiol. Infect. 124, 599-602. https://doi.org/10.1017/
S0950268899003969
Ezeh, A.O., Addo, P.B., Adesiyun, A.A., Bello, C.S., Makinde, A.A.,
Serological prevalence of bovine leptospirosis in Plateau State,
Nigeria. Rev. Elev. Med. Vet. Pays Trop. 42, 505-508.
Fávero, J.F., de Araújo, H.L., Lilenbaum, W., Machado, G., Tonin, A.A.,
Baldissera, M.D., Stefani, L.M., Da Silva, A.S., 2017. Bovine leptospirosis:
Prevalence, associated risk factors for infection and their
cause-effect relation. Microb. Pathog. 107, 149-154. https://doi.
org/10.1016/j.micpath.2017.03.032
Feresu, S.B., 1987. Serological survey of leptospiral antibodies in cattle
in Zimbabwe. Trop. Anim. Health Prod. 19, 209-214. https://doi.
org/10.1007/BF02242118
Fuente, V.C. la, Zapata-Campos, C., Loredo-Osti, J., López-Zavala, R.,
Jasso-Obregón, J.O., Martínez-Bautista, E., 2012. Seroprevalence
and Risk Factors associated with Leptospirosis (L. interrogans) in
Bovine Cattle in Northeastern Mexico. Thai J. Vet. Med. 42, 7-12.
Hesterberg, U.W., Bagnall, R., Bosch, B., Perrett, K., Horner, R., Gummow,
B., 2009. A serological survey of leptospirosis in cattle of rural
communities in the province of KwaZulu-Natal, South Africa. J. S.
Afr. Vet. Assoc. 80, 45-49. https://doi.org/10.4102/jsava.v80i1.168
Ismail, Z.B., Abutarbush, S.M., Al-Majali, A.M., Gharaibeh, M.H., Al-
Khateeb, B., 2019. Seroprevalence and risk factors of Leptospira serovar
Pomona and Leptospira serovar Hardjo infection in dairy cows
in Jordan. J. Infect. Dev. Ctries. 13, 473-479. https://doi.org/10.3855/
jidc.11146
Jimenez-Coello, M., Vado-Solis, I., Cárdenas-Marrufo, M.F.,
Rodríguez-Buenfil, J.C., Ortega-Pacheco, A., 2008. Serological survey
of canine leptospirosis in the tropics of Yucatan, Mexico using
two different tests. Acta Trop. 106, 22-26. https://doi.org/10.1016/j.
actatropica.2007.12.011
Khalili, M., Sakhaee, E., Afatoonian, M.R., Abdollahpour, G., Tabrizi,
S.S., Damaneh, E.M., Hossini-nasab, S., 2014. Seroprevalence of
bovine leptospiral antibodies by microscopic agglutination test in
Southeast of Iran. Asian Pac. J. Trop. Biomed. 4, 354-357. https://doi.
org/10.12980/APJTB.4.2014C1206
Kirkwood, B. R. & Sterne, J.A.., 2003. Essential medical statistics. Blackwell
Science.
Kocabiyik, A.. & C.C., 2004. Bovine leptospirosis in south Marmara
region of Turkey: A serological survey. Rev. Médecine Vétérinaire,
, 606-608.
Leon, L.L., Garcia, R.C., Diaz, C.O., Valdez, R.B., Carmona, G.C.A.,
Velazquez, B.L.G., 2008. Prevalence of leptospirosis in dairy cattle
from small rural production units in Toluca Valley, State of Mexico.
Ann. N. Y. Acad. Sci. 1149, 292-295. https://doi.org/10.1196/annals.
002
Levett, P.N., 2015. Systematics of Leptospiraceae. Curr. Top. Microbiol.
Immunol. 387, 11-20. https://doi.org/10.1007/978-3-662-45059-8_2
Levett, P.N., 2001. Leptospirosis. Clin. Microbiol. Rev. 14, 296-326.
Lindahl, E., Boqvist, S., Artursson, K., Magnusson, U., 2011. A fieldstudy
on Leptospira seroprevalence in dairy cows in four geographical
areas in Sweden. Acta Vet. Scand. 53, 2009-2012. https://doi.
org/10.1186/1751-0147-53-53
Machang’u, R.S., Mgode, G., Mpanduji, D., 1997. Leptospirosis in animals
and humans in selected areas of Tanzania. Belgian J. Zool. 127
Suppl, 97-104.
Mariya, R., Chaudhary, P., Kumar, A.A., Thangapandian, E., Amutha, R.,
Srivastava, S.K., 2006. Evaluation of a recombinant LipL41 antigen
of Leptospira interrogans serovar Canicola in ELISA for serodiagnosis
of bovine Leptospirosis. Comp. Immunol. Microbiol. Infect. Dis.
, 269-277. https://doi.org/10.1016/j.cimid.2006.06.007
Miller, D.A., Wilson, M.A., Beran, G.W., 1991. Survey to estimate prevalence
of Leptospira interrogans infection in mature cattle in the United
States. Am. J. Vet. Res. 52, 1761-1765.
Naudet, J., Crespin, L., Cappelle, J., Kodjo, A., Ayral, F., 2022. Circulating
serogroups of Leptospira in swine from a 7-year study in France
(2011-2017). Porc. Heal. Manag. 8, 1-9. https://doi.org/10.1186/
s40813-022-00257-y
Parvez, M.A. Prodhan, M.A.M., Rahman M.A. and Faruque, M.R., 2015.
Seroprevalence and Associated Risk Factors of Leptospira interrogans
Serovar Hardjo in Dairy Cattle of Chittagong, Bangladesh. Pak.
Vet. J. 35, 350-354.
Prescott, J.F., Miller, R.B., Nicholson, V.M., Martin, S.W., & L.T., 1988.
Seroprevalence association with abortion of leptospirosis in cattle in
Ontario. Can. Vet. J. 52, 210-215.
Pritchard, D.G., 1986. The present state of leptospirosis diagnosis and
control, Martinus N. ed. Netherlands.
Rocha, T., 1998. A review of leptospirosis in farm animals in Portugal.
Rev. Sci. Tech. 17, 699-712. https://doi.org/10.20506/rst.17.3.1128
Ruano, M.P., Burgos Macías, D.I., Goicochea, C.A.B., Zambrano
Aguayo, M.D., Sandoval Valencia, H.P., Falconi Flores, M.A., Vera
Loor, L.A., Revelo Ruales, A.P., Fonseca-Rodríguez, O., 2020. Seroprevalence
and risk factors of bovine leptospirosis in the province of
Manabí, Ecuador. Comp. Immunol. Microbiol. Infect. Dis. 72. https://
doi.org/10.1016/j.cimid.2020.101527
Sakhaee, E., pour, G.R.A., 2011. Detection of leptospiral antibodies by microscopic
agglutination test in north-east of Iran. Asian Pac. J. Trop.
Biomed. 1, 227-229. https://doi.org/10.1016/S2221-1691(11)60032-
Salgado, M., Otto, B., Sandoval, E., Reinhardt, G., Boqvist, S., 2014. A
cross-sectional observational study to estimate herd level risk factors
for Leptospira spp. serovars in small holder dairy cattle farms in
southern Chile. BMC Vet. Res. 10, 2-7. https://doi.org/10.1186/1746-
-10-126
Sankar, S., Harshan, H.M., Somarajan, S.R., Srivastava, S.K., 2010.
Evaluation of a recombinant LigB protein of Leptospira interrogans
serovar Canicola in an enzyme-linked immunosorbent assay for the
serodiagnosis of bovine leptospirosis. Res. Vet. Sci. 88, 375-378.
Schoonman, L., Swai, E.S., 2010. Herd- and animal-level risk factors for
bovine leptospirosis in Tanga region of Tanzania. Trop. Anim. Health
Prod. 42, 1565-1572. https://doi.org/10.1007/s11250-010-9607-1
Segura-Correa, J.C., Domínguez-Díaz, D., Avalos-Ramírez, R., Argaez-
Sosa, J., 2010. Intraherd correlation coefficients and design
effects for bovine viral diarrhoea, infectious bovine rhinotracheitis,
leptospirosis and neosporosis in cow-calf system herds in North-eastern
Mexico. Prev. Vet. Med. 96, 272-275. https://doi.org/10.1016/j.
prevetmed.2010.07.006
Strutzberg-Minder, K., Tschentscher, A., Beyerbach, M., Homuth, M.,
Kreienbrock, L., 2018. Passive surveillance of Leptospira infection in
swine in Germany. Porc. Heal. Manag. 4, 1-8. https://doi.org/10.1186/
s40813-018-0086-5
Subathra, M., Senthilkumar, T.M.A., Ramadass, P., Dhinakar Raj, G.,
Development of rapid flow-through-based dot-immunoassay
for serodiagnosis of leptospirosis in dogs. Comp. Immunol. Microbiol.
Infect. Dis. 34, 17-22. https://doi.org/10.1016/j.cimid.2009.10.003
Suepaul, S.M., Carrington, C. V., Campbell, M., Borde, G., Adesiyun,
A.A., 2011. Seroepidemiology of leptospirosis in livestock in Trinidad.
Trop. Anim. Health Prod. 43, 367-375. https://doi.org/10.1007/
s11250-010-9698-8
Tagliabue, S., Figarolli, B.M., D’Incau, M., Foschi, G., Gennero, M.S.,
Giordani, R., Natale, A., Papa, P., Ponti, N., Scaltrito, D., Spadari, L.,
Vesco, G., Ruocco, L., 2016. Indagine sierologica sulla presenza di Leptospira spp. in Italia: Dati nazionali 2010-2011. Vet. Ital. 52, 129-
Thrusfield, M., 2007. Veterinary Epidemiology, 3rd ed. Oxford, London.
Vincent, C.T., Munger, C., Sylvestre, F. & Levesque, I.J., 2007. La leptospirose
dans le cheptel québécois en 2006 : Bulletin Zoosanitaire.
Réseau d’Alerte d’information Zoosanit. 2005, 1-8.
Vincent, A.T., Schiettekatte, O., Goarant, C., Neela, V.K., Bernet, E.,
Thibeaux, R., Ismail, N., Khalid, M.K.N.M., Amran, F., Masuzawa,
T., Nakao, R., Korba, A.A., Bourhy, P., Veyrier, F.J., Picardeau, M.,
Revisiting the taxonomy and evolution of pathogenicity of the
genus Leptospira through the prism of genomics. PLoS Negl. Trop.
Zaidi, S., Bouam, A., Bessas, A., Hezil, D., Ghaoui, H., Ait-Oudhia, K.,
Drancourt, M., Bitam, I., 2018. Urinary shedding of pathogenic leptospira
in stray dogs and cats, Algiers: A prospective study. PLoS One
Zar, J.H., 1999. Biostatistical analysis, 5th Edition. Ed. Prentice Hall.
