Effect of dried and ensiled pomegranate by-pr oduct on performance, blood metabolites and meat quality of Balochi lambs
Abstract
This study aimed to investigate the addition of dried and ensiled pomegranate by-products on the performance, rumen parameters, blood metabolites and meat quality of Balochi ram lambs. A total of 18 lambs at 90 d age with similar body weight (BW, 28.0±3.5 kg) with 6 replicates per treatment were used. The experimental treatments including 1) Control, 2) 20% of dried pomegranate by-product, 3) 20% of ensiled pomegranate by-product. According to the results, it was found that the use of pomegranate by-products does not have a significant effect on daily weight gain, feed consumption and feed conversion ratio (P>0.05). Except the constant rate of gas production, other rumen parameters were significantly affected by the experimental treatments (P<0.05). Furthermore, the use of pomegranate by-products had a significant effect on blood cholesterol (P<0.05). Moreover, the dietary treatments have no significant effect on meat traits and its components (P>0.05). The overall results showed that ensiling pomegranate by-products could reduce the amount of tannin in them to a greater extent.
Article Details
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Hashemi Kondori, S., Vakili, R., & Ghiasi, S. (2025). Effect of dried and ensiled pomegranate by-pr oduct on performance, blood metabolites and meat quality of Balochi lambs. Journal of the Hellenic Veterinary Medical Society, 76(2), 9237–9246. https://doi.org/10.12681/jhvms.38760
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Riferimenti bibliografici
Abarghuei MJ, Rouzbehan Y, Salem A, Zamiri MJ (2013) Nutrient
digestion, ruminal fermentation and performance of dairy cows
fed pomegranate peel extract. Livestock Science 157(2-3):452-461.
Ahooei GR, Foroughi AR., Tahmasbi AM, Shahdadi AR, Vakili R (2011).
Effects of Different Levels of Dried Citrus Pulp and Urea on Performance
of Fattening Male Calves. Journal of animal and veterinary
advances, 10(14), 1811-1816. 10.3923/javaa.2011.1811.1816.
Al-Hadithy H (2013) Estimation of serum liver enzymes activities in
Awassi sheep. The Iraqi Journal of Veterinary Medicine 37(1):115-
Alhidary I, Abdelrahman M (2016) Effects of naringin supplementation
on productive performance, antioxidant status and immune response
in heat-stressed lambs. Small Ruminant Research 138:31-36. https://
Besharati M, Maggiolino A, Palangi V, Kaya A, Jabbar M, Eseceli H, De
Palo P, Lorenzo JM (2022) Tannin in ruminant nutrition. Molecules.
Nov 27;27(23):8273. https://doi.org/10.3390/molecules27238273.
Broderick G, Kang J (1980) Automated simultaneous determination of
ammonia and total amino acids in ruminal fluid and in vitro media.
Journal of dairy science 63(1):64-75. https://doi.org/https://doi.
org/10.3168/jds.S0022-0302(80)82888-8.
Carulla J, Kreuzer M, Machmüller A, Hess H (2005) Supplementation
of Acacia mearnsii tannins decreases methanogenesis and urinary
nitrogen in forage-fed sheep. Australian journal of agricultural
research 56(9):961-970. https://doi.org/https://doi.org/10.1071/
AR05022.
Chemists AoOA, Chemists AoOA. (1931). Official methods of analysis
of the Association of Official Analytical Chemists (Vol. 3). Association
of Official Analytical Chemists.
De Boever J, Aerts J, Vanacker J, De Brabander D (2005) Evaluation of
the nutritive value of maize silages using a gas production technique.
Animal Feed Science and Technology 123:255-265. https://doi.org/
Dentinho M, Paulos K, Francisco A, Belo A, Jerónimo E, Almeida J,
Bessa R, Santos-Silva J (2020) Effect of soybean meal treatment
with Cistus ladanifer condensed tannins in growth performance,
carcass and meat quality of lambs. Livestock Science 236:104021.
El-Hamamsy S, El-Khamissi H (2020) Phytochemicals, antioxidant
activity and identification of phenolic compounds by HPLC of
pomegranate (Punica granatum L.) Peel extracts. Journal of Agricultural
chemistry and biotechnology 11(4):79-84. https://doi.org/
Emami A, Nasri MF, Ganjkhanlou M, Rashidi L, Zali A (2015a) Dietary
pomegranate seed pulp increases conjugated-linoleic and-linolenic
acids in muscle and adipose tissues of kid. Animal Feed Science
and Technology 209:79-89. https://doi.org/https://doi.org/10.1016/j.
anifeedsci.2015.08.009.
Emami A, Nasri MF, Ganjkhanlou M, Zali A, Rashidi L (2015b) Effects
of dietary pomegranate seed pulp on oxidative stability of kid meat.
Meat science 104:14-19. https://doi.org/https://doi.org/10.1016/j.
meatsci.2015.01.016.
Frutos P, Mantecón ÁR, Angulo GH, García FJG (2004) Tannins and ruminant
nutrition. Spanish journal of agricultural research 2(2):191-
Gee J, Wortley G, Johnson I, Price K, Rutten A, Houben G, Penninks A
(1996) Effects of saponins and glycoalkaloids on the permeability
and viability of mammalian intestinal cells and on the integrity
of tissue preparations in vitro. Toxicology in vitro 10(2):117-128.
Getachew G, Robinson P, DePeters E, Taylor S (2004) Relationships
between chemical composition, dry matter degradation and in vitro
gas production of several ruminant feeds. Animal Feed Science and
Technology 111(1-4):57-71. https://doi.org/https://doi.org/10.1016/
S0377-8401(03)00217-7.
Grainger C, Clarke T, Auldist M, Beauchemin K, McGinn S, Waghorn
G, Eckard RJ (2009) Potential use of Acacia mearnsii condensed
tannins to reduce methane emissions and nitrogen excretion from
grazing dairy cows. Canadian Journal of Animal Science 89(2):241-
Guerreiro O, Alves SP, Soldado D, Cachucho L, Almeida JM, Francisco
A, Santos-Silva J, Bessa RJ, Jerónimo E (2020) Inclusion of the
aerial part and condensed tannin extract from Cistus ladanifer L. in
lamb diets–Effects on growth performance, carcass and meat quality
and fatty acid composition of intramuscular and subcutaneous fat.
Meat science 160:107945. https://doi.org/https://doi.org/10.1016/j.
meatsci.2019.107945.
Ibrahim M, Arafa SA, Hassan FA, Zaki EE (2017) Utilization of pomegranate
(Punica granatum L.) by-product powder as a natural growth
promoter in growing rabbit diets. Egyptian Journal of Rabbit
Science 27(2):197-217. https://doi.org/https://doi.org/10.21608/
ejrs.2017.46568.
Johanningsmeier SD, Harris GK (2011) Pomegranate as a functional
food and nutraceutical source. Annual review of food science and
technology 2(1):181-201. https://doi.org/https://doi.org/10.1146/
annurev-food-030810-153709.
Kh M (1988) Estimation of the energetic feed value obtained from
chemical analysis and in vitro gas production using rumen fluid.
Anim Res Dev 28:7-55.
Koohmaraie M (1996) Biochemical factors regulating the toughening
and tenderization processes of meat. Meat science 43:193-201.
Kotsampasi B, Christodoulou V, Zotos A, Liakopoulou-Kyriakides M,
Goulas P, Petrotos K, Natas P, Bampidis V (2014) Effects of dietary
pomegranate byproduct silage supplementation on performance,
carcass characteristics and meat quality of growing lambs. Animal
Feed Science and Technology 197:92-102. https://doi.org/https://
doi.org/10.1016/j.anifeedsci.2014.09.003.
Liu W, Ding H, Erdene K, Chen R, Mu Q, Ao C (2018) Effects of
flavonoids from Allium mongolicum Regel as a dietary additive
on meat quality and composition of fatty acids related to flavor in
lambs. Canadian Journal of Animal Science 99(1):15-23. https://
Matthews C, Crispie F, Lewis E, Reid M, O’Toole PW, Cotter PD (2019)
The rumen microbiome: a crucial consideration when optimising
milk and meat production and nitrogen utilisation efficiency. Gut
microbes. Mar 4;10(2):115-32. https://doi.org/10.1080/19490976
.2018.1505176.
Mergeduš A, Pšenková M, Brus M, Janžekovič M (2018) Tannins
and their effect on production efficiency of ruminants. Agricultura
Scientia.15(1/2):1-1. https://doi.org/10.18690/agricultura.
1-2.1-11.2018.
Miguel MG, Neves MA, Antunes MD (2010) Pomegranate (Punica
granatum L.): A medicinal plant with myriad biological properties-A
short review. Journal of Medicinal Plants Research 4(25):2836-
Min B, Barry T, Attwood G, McNabb W (2003) The effect of condensed
tannins on the nutrition and health of ruminants fed fresh temperate
forages: a review. Animal Feed Science and Technology 106(1-4):3-
Muhammed S, Stewart C, Acamovic T. (1994). Effects of tannic acid
on cellulose degradation, adhesion and enzymic activity of rumen
microorganisms. Proceedings of the Society of Nutrition Physiology
(Germany).
Nasri S, Salem HB (2012) Effect of oral administration of Agave americana
or Quillaja saponaria extracts on digestion and growth of
Barbarine female lamb. Livestock Science 147(1-3):59-65.
Natalello A, Luciano G, Morbidini L, Valenti B, Pauselli M, Frutos
P, Biondi L, Rufino-Moya PJ, Lanza M, Priolo A (2019) Effect
of feeding pomegranate byproduct on fatty acid composition of
ruminal digesta, liver, and muscle in lambs. Journal of agricultural
and food chemistry 67(16):4472-4482. https://doi.org/https://doi.
org/10.1021/acs.jafc.9b00307.
Newbold C, Lassalas B, Jouany J (1995) The importance of methanogens
associated with ciliate protozoa in ruminal methane production in
vitro. Letters in applied microbiology 21(4):230-234. https://doi.
Newbold C, McIntosh F, Williams P, Losa R, Wallace R (2004) Effects of
a specific blend of essential oil compounds on rumen fermentation.
Animal Feed Science and Technology 114(1-4):105-112. https://doi.
Omer HA, Abdel-Magid SS, Awadalla IM (2019) Nutritional and chemical
evaluation of dried pomegranate (Punica granatum L.) peels
and studying the impact of level of inclusion in ration formulation
on productive performance of growing Ossimi lambs. Bulletin of
the National Research Centre 43:1-10. https://doi.org/https://doi.
org/10.1186/s42269-019-0245-0.
Patra AK, Saxena J (2011) Exploitation of dietary tannins to improve
rumen metabolism and ruminant nutrition. Journal of the Science of
Food and Agriculture. Jan 15;91(1):24-37. https://doi.org/10.1002/
jsfa.4152.
Pimentel P, Pellegrini C, Lanna DPD, Brant L, Ribeiro C, Silva T,
Barbosa A, da Silva Júnior J, Bezerra L, Oliveira R (2021) Effects
of Acacia mearnsii extract as a condensed-tannin source on animal
performance, carcass yield and meat quality in goats. Animal Feed
Science and Technology 271:114733. https://doi.org/https://doi.
org/10.1016/j.anifeedsci.2020.114733.
Priolo A, Micol D, Agabriel J (2001) Effects of grass feeding systems
on ruminant meat colour and flavour. A review. Animal research
(3):185-200. https://doi.org/https://doi.org/10.1051/animres:
Reed J, Soller H, Woodward A (1990) Fodder tree and straw diets
for sheep: intake, growth, digestibility and the effects of phenolics
on nitrogen utilisation. Animal Feed Science and Technology
(1-2):39-50. https://doi.org/https://doi.org/10.1016/0377-
(90)90050-I.
Rodríguez R, De la Fuente G, Gómez S, Fondevila M (2013) Biological
effect of tannins from different vegetal origin on microbial
and fermentation traits in vitro. Animal Production Science. Nov
;54(8):1039-46. https://doi.org/10.1071/AN13045.
Sadq SM, Ramzi DO, Hamasalim HJ, Ahmed KA (2016) Growth performance
and digestibility in Karadi lambs receiving different levels
of pomegranate peels. Open Journal of Animal Sciences 6(01):16.
Salami SA, Luciano G, O’Grady MN, Biondi L, Newbold CJ, Kerry
JP, Priolo A (2019) Sustainability of feeding plant by-products: A
review of the implications for ruminant meat production. Animal
Feed Science and Technology 251:37-55. https://doi.org/https://
doi.org/10.1016/j.anifeedsci.2019.02.006.
Salem A, López S, Ranilla MJ, González JS. (2011). Influence of quebracho
tannins administration on some blood metabolites in sheep
and goats. In Challenging strategies to promote the sheep and goat
sector in the current blobal context (Vol. 99, pp. 273-278). CIHEAM,
FAO Zaragoza.
Shabtay A, Eitam H, Tadmor Y, Orlov A, Meir A, Weinberg P, Weinberg
ZG, Chen Y, Brosh A, Izhaki I (2008) Nutritive and antioxidative
potential of fresh and stored pomegranate industrial byproduct as
a novel beef cattle feed. Journal of agricultural and food chemistry
(21):10063-10070. https://doi.org/https://doi.org/10.1021/
jf8016095.
Taher-Maddah M, Maheri-Sis N, Salamatdoustnobar R, Ahmadzadeh A
(2012) Comparing nutritive value of ensiled and dried pomegranate
peels for ruminants using in vitro gas production technique.
Tavendale MH, Meagher LP, Pacheco D, Walker N, Attwood GT, Sivakumaran
S (2005) Methane production from in vitro rumen incubations
with Lotus pedunculatus and Medicago sativa, and effects of
extractable condensed tannin fractions on methanogenesis. Animal
Feed Science and Technology 123:403-419. https://doi.org/https://
doi.org/10.1016/j.anifeedsci.2005.04.037.
Velasco V, Williams P (2011) Improving meat quality through natural
antioxidants. Chilean journal of agricultural research 71(2):313-322.
Watanabe A, Daly C, Devine C (1996) The effects of the ultimate pH of
meat on tenderness changes during ageing. Meat science 42(1):67-
Yanez Ruiz D, Moumen A, Martin Garcia A, Molina Alcaide E (2004)
Ruminal fermentation and degradation patterns, protozoa population,
and urinary purine derivatives excretion in goats and wethers
fed diets based on two-stage olive cake: Effect of PEG supply.
Journal of animal science 82(7):2023-2032. https://doi.org/https://
doi.org/10.2527/2004.8272023x.
