Impacts of pasteurized buffalo milk on pr oliferation of Hela cells: Role of Caspase-3, Caspase-9, and P53 genes

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Published: Jul 5, 2025
DOI: https://doi.org/10.12681/jhvms.39194
Keywords:
Buffalo milk Hela cells Apoptosis p53 Caspases
Halla E.K. El-Bahgy
Huazhong Agricultural University, College of Animal Science and Technology, National Center for International Research, Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Education, Wuhan, 430070, People’s Republic of China.
A Abulaiti
Huazhong Agricultural University, College of Animal Science and Technology, National Center for International Research, Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Education, Wuhan, 430070, People’s Republic of China.
MM Soliman
Department of Clinical Laboratory Sciences, Taif University, Turabah University College P.O. Box 11099, Taif 21944, Saudi Arabia
F Althobaiti
Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
A Elshehawi
Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
L Yang
Huazhong Agricultural University, College of Animal Science and Technology, National Center for International Research, Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Education, Wuhan, 430070, People’s Republic of China
Abstract

 Buffalo milk is characterized by its high nutritional value, and there is a significant association between excessive consumption of commercially available buffalo milk and the development of various cancer types. The current study was designed to assess how buffalo milk affects the growth and viability of Hela cells, a cervical cancer cell line, as well as to investigate the molecular mechanism behind these effects. Pasteurized-buffalo milk was added to cells at 0% (negative control), 0.00025%, 0.0005%, 0.001%, 0.0015, 0.002%, 0.0025%, 0.005%, and positive control (H2O2) for 24 hours. Results indicated that the growth of Hela cells was notably boosted as the concentration of buffalo milk increased. The buffalo milk reduced the apoptosis of Hela cells and increased the viability of cells. Moreover, Caspase-3 and Caspase-9 activities were stimulated in treated Hela cells, and significantly increased with 0.001% of buffalo milk, compared to positive control cells. In contrast, buffalo milk treatment for 24 hours showed substantial reduction in P53 gene expression within Hela cells. In conclusion, buffalo milk increases the proliferation and viability of Hela cells by decreasing the apoptosis and cell viability of examined cells. Furthermore, it induces upregulation in Caspase-3 and Caspase-9 expressions while deactivating the expression of P53 gene.

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