Polymorphism of Prolactin Gene and Its Association with Egg Production Trait in Four Commercial Chicken Lines

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Опубликован: Ιαν 29, 2018
DOI: https://doi.org/10.12681/jhvms.15502
MOHAMED M.M. OSMAN
Department of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University
SHAABAN A. HEMEDA
Department of Animal Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Alexandria University
ABEER A.I. HASSANIN
Department of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University
WALAA A. HUSSEINY
Department of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University
Аннотация

Broodiness is a behavioral trait observed in most common breeds of domestic fowl and due to its fundamental role in avian reproduction, it has been of great interest to poultry scientists, breeders and producers of hatching eggs. Prolactin gene (PRL) is generally accepted as crucial to the onset and maintenance of broodiness in birds and thus plays a crucial role in egg production. Therefore, the present study aimed to screen the Single Nucleotides Polymorphisms (SNPs) of prolactin gene in four commercial chicken lines namely Hubbard F15, Lohmann, Cobb500, and Avian48 using PCR and direct sequencing. A total number of forty chickens (ten females from each of the four commercial chicken lines) were used. Blood samples were collected aseptically from brachial (wing) vein of the chickens for genomic DNA extraction. PCR reaction was done using five pairs of primers, one sense (F) and one antisense (R) primer for each of the five exons of prolactin gene. Finally, DNA sequencing and Single Nucleotide Polymorphisms (SNPs) analysis was done using Laser gene Megalign program. The results showed three SNPs in Hubbard F15 chicken line; one synonymous SNP at the position 3838 bp (ACC/ACT-transition) in exon 2 while in exon 5, two SNPs were detected; one non-synonymous single nucleotide polymorphism at the position 7921bp (CCT/TCT-transition) which results in amino acid changes at codon positions 169 (P/S), and one synonymous single nucleotide polymorphism at the position 8187 bp T/ C. The study concluded that this SNP in PRL gene could be used as the potential molecular markers for egg production traits in chicken.

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Библиографические ссылки
Alipana M., shojaian K. and Bandani H.Kh. 2011 the polymorphism of prolactin gene in native chicken Zabol region. Journal of animal and veterinary advances. 10 (5), 619-621.
Andreas U., Harm N., Xiangyu R., Ton B., René G. and Jack A.M. L. 2007 Primer3Plus, an enhanced web interface to Primer3. Nucleic Acids Res. 35, 71–74.
Au W. L. and Leung F. C.C. 2002 Rapid communication: complete nucleotide sequence of the chicken prolactin gene. Journal of Animal Science. 80,1381.
Begli H. E., Zerehdaran S., Hassani S., Abbasi M. A. and Ahmadi A. K. 2010 Polymorphism in prolactin and PEPCK-C genes and its association with economic traits in native fowl of Yazd province. Iranian Journal of Biotechnology. Vol. 8, No. 3.
BhattacharyaT.K., Chatterjee R.N., Sharma R.P., Rajkumar U., Niranjan M. and Reddy B.L.N. 2011 Association of polymorphism in the prolactin promoter and egg quality traits in laying hens. British Poultry Science. Vol. 52, No. 5, pp. 551—557
Bonomo I.T., Lisboa P.C., Pereira A.R., Passos M.C. and de Moura E.G. 2007 Prolactin inhibition in dams during lactation programs for overweight and leptin resistance in adult offspring. J. Endocrinol. 192, 339-344.
Chen H. Q., Wei H. Q., Qin J. and Chen H. 2011 The Novel Genetic Change in 5’-untranslated Region of Goose Prolactin Gene and their Distribution Pattern in Different Goose Breeds. Asian Journal of Animal & Veterinary Advances. 6 (11), 1069.
Cui J. X., Du H. L., Liang Y., Deng X. M., Li N. and Zhang X. Q. 2006 Association of Polymorphisms in the Promoter Region of Chicken Prolactin with Egg Production. Poultry Science. 85,26–31.
Ding-guo C., Fu-wei L., Hai-xia H., Qiu-xia L., Yan Z., Gui-ming L. et al. 2010 SNP Detection of Exon 2 of PRL Gene and its Associations with Egg- laying and Broodiness Traits in ShiqizaChicken.China animal husbandry & veterinary medicine.Vol. 37 (2), 104-107
Duncan D.B. 1955 Multiple ranges and multiple f-test Biomet. 11,1.
Elsholtz H.P., Lew A.M., Albert P.R. and Sundmark V.C. 1991 Inhibitory control of prolactin and Pit-1 gene promoters by dopamine. J Biol Chem. 266, 22919–25.
El-Tarabany M.S., Awad A. and El-Bayomi K.M. 2014 Genetic Polymorphism of Prolactin, Bone Morphogenetic Protein Receptor 1B and Insulin- like Growth Factor 1 Genes in Two Selected Lines of Japanese Quail. Life Science Journal. 11 (6).
Erehehuara 2003 Studies on the SNP of the chicken’s prolactingene. Ph.D. Thesis, Huazhong Agricultural University.
Fu-wei L., Hai-xia H., Qiu-xia L., Yan Z., Jin-bo g., Wei L. et al. 2014 Exon 2 SNP Detection of PRL Gene and its Associations with Egg Production Traits in Wenshang Barred Chicken.China animal husbandry & veterinary medicine.Vol. 41 (9), 184-188.
Jiang R.S., Xu G. Y., Zhang X. Q. and Yang N. 2005 Association f Polymorphisms for Prolactin and Prolactin Receptor Genes with Broody Traits in Chickens. Poultry Science. 84,839–845.
Jiang R.S., ZhangL.L., Geng Z.Y., Yang T. and Zhang S.S. 2009 Single nucleotide polymorphisms in the 5’-flanking region of the prolactin gene and the association with reproduction traits in geese. South African Journal of Animal Science. 39.
Kansaku N., Hiyama G., Sasanami T. and Zadworny D. 2008 Prolactin and Growth Hormone in Birds: Protein Structure, Gene Structure and Genetic Variation. The Journal of Poultry Science. 45,1-6.
Kulibaba R. A. and Podstreshnyi A. P. 2012 Prolactin and Growth Hormone Gene Polymorphisms in Chicken Lines of Ukrainian Selection Cytology and Genetics. Vol. 46, No. 6, pp. 390–395.
Li H.F., Shu J.T., Du Y.F., Shan Y.J., Chen K.W., Zhang X.Y., et al. 2013 Analysis of the genetic effects of prolactin gene polymorphisms on 404 chicken egg production. MolBiol Rep. 40,289–294.
Liang Y., Cui J., Yang G., Leung F.C.C. and Zhanga X. 2006 Polymorphisms of 5 flanking region of chicken prolactin gene. Domestic Animal Endocrinology. 30, 1–16.
Liu Z., Shiz D., Liu Y., Lim Y., Huangy M. and Yao B.H. 2008 Molecular cloning and characterization of the Magang goose prolactin gene. Gen. Comp.Endocrinol.155, 208-216.
Muladno 2010 TeknologiRekayasaGenetika. Edisikedua. Bogor (Indones): IPB Press.
Nestor K.E. 1980 Genetics of growth and reproduction in the turkey. 7. Relationship of total egg production, intensity of lay, broodiness, and body weight. PoultSci. 59,1385–1394.
Ohkubo T., Tanaka M. and Nakashima K. 2000 Molecular cloning of the chicken prolactin gene and activation by pit-1 and cAMP-induced factor in GH3 cells. General and comparative endocrinology. 119, 208-216.
Petsko G.A. and Ringe D. 2004 Protein Structure and Function. London (UK), New Science Press.
Power D.M. 2005 Developmental ontogeny of prolactin and its receptor in fish. Gen. Comp. Endocrinol. 142, 25-33.
Rashidi H., Rahimi-Mianji G., Farhadiand A. and Gholizadeh M. 2012 Association of prolactin and prolactin receptor gene polymorphisms with economic traits in breeder hens of indigenous chickens of Mazandaran province. Iranian J. Bio.Thech. 10, 129-135.
Reddy I.J., David C.G., Sarma P.V. and Singh K. 2002 The possible role of prolactin in laying performance and steroid hormone secretion in domestic hen (Gallus domesticus). Gen. Comp. Endocrinol. 127, 249-255.
Salam Al Karadaghi 2015 Basics of Protein structure: The 20 Amino Acids and their role in Protein structures. Sambrook J. and Russell D.W. 2001 Molecular Cloning: A Laboratory Manual. 3rd Edn., Cold Spring Harbor Lab (CSHL), New York, ISBN: 0879695773.
SAS institute 1998 SAS statistical guide for personal computer, SAS institute Inc. Cary, NC.
Sharp P.J., MacNamee M.C., Talbot R.T., Sterling R.J. and Hall T.R. 1984 Aspects of the neuroendocrine control of ovulation and broodiness in the domestic hen. J ExpZool. 232,475–483.
Wang C., Liang Z., Yu W., Feng Y., Peng X., Gong Y. et al. 2011 Polymorphism of the prolactin gene and its association with egg production traits in native Chinese ducks. South African Journal of Animal Science. 41 (no 1).
Wilkanowska A., Mazurowski A., Mroczkowski S. and Kokoszyñski D. 2014 Prolactin (PRL) and Prolactin Receptor (PRLR) Genes and their Role in Poultry Production Traits. Institute of Systematics and Evolution of Animals, PAS, Kraków. Folia Biologica (Kraków). vol. 62, No1.
Wu X., Li A., Wang S.K., Wang H., Jia H.Y. and Wang G.Y. 2008 Correlation analysis between nucleotide polymorphism of the prolactin exon 5 and broodiness in ducks. Fujian J. Anim. Husband. Vet. 30, 6-9.
Xu H. ShenX., Zhou M., Fang M., Zeng H., Nie Q. et al. 2010 The genetic effects of the dopamine D1 receptor gene on chicken egg production and broodiness traits. BMC Genetics. 11,17.
Yan L., Fu-wei L., Ding-guo C., Qiu-xia L., Hai-xia H., Yan Z., et al. 2011 SNP Detection of Exon 4 of Prolactin Gene and its Associations with Egg-laying and Broodiness Traits in Shiqiza Chicken. China Animal Husbandry & Veterinary Medicine. Vol. 38, Issue (3), 146-149.
Yousefi S., Raoufi Z., Rasouli Z. and Zerehdaran S. 2012 Investigation of Prolactin Gene Polymorphism in Japanese Quail. Animal Science and Biotechnologies. 45 (1).