Relationship of the bovine IGF1, TG, DGAT1 and MYF5 genes to meat colour, tenderness and cooking loss


Keywords:
Genetic markers meat quality single nucleotide polymorphisms marker-assisted selection
S. ARDICLI
H. SAMLI
D. DINCEL
B. EKIZ
H. YALCINTAN
B. VATANSEVER
F. BALCI
Abstract

Bovine insulin-like growth factor 1 (IGF1), thyroglobulin (TG), diacylglycerol-O-acyltransferase 1 (DGAT1) and myogenic factor 5 (MYF5) genes play an important role in the physiology of lipid and muscle metabolism and are therefore considered as candidate genes for meat production traits in farm animals. The objectives of this study were to investigate single nucleotide polymorphisms (SNPs) in IGF1, TG, DGAT1 and MYF5 genes and to evaluate whether these polymorphisms affected meat colour, tenderness and cooking loss in Holstein cattle. Initially, the SNPs were detected by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) method. Meat samples (N= 50) derived from M. longissimus thoracis et lumborum (LTL) were used in the current study. Significant differences in variations of meat colour parameters were observed at 24 hours post-mortem. IGF1 was associated with colour parameters of a* and chroma values. In addition, effects of TG were statistically significant on L* and a* values, while, effects of MYF5 were significant on a* value. There was no association of the tested SNPs with meat pH, tenderness and cooking loss. The results presented here may give the valuable information for improving meat colour in cattle.

Article Details
  • Section
  • Research Articles
Downloads
Download data is not yet available.
References
Banos G, Woolliams J, Woodward B, Forbes A, Coffey M (2008) Impact of single nucleotide polymorphisms in leptin, leptin receptor, growth hormone receptor, and diacylglycerol acyltransferase (DGAT1) gene loci on milk production, feed, and body energy traits of UK dairy cows. J Dairy Sci 91: 3190-3200.
Barendse W, Bunch R, Thomas M, Armitage S, Baud S, Donaldson N (2001) The TG5 DNA marker test for marbling capacity in Australian feedlot cattle. Beef Quality CRC, Marbling Symposium, Coffs Harbor. pp. 52-57.
Barendse W, Bunch R, Thomas M, Armitage S, Baud S, Donaldson N (2004) The TG5 thyroglobulin gene test for a marbling quantitative trait loci evaluated in feedlot cattle. Anim Prod Sci 44:669-674.
Barendse WJ (1999) Assessing lipid metabolism. International Patent Application PCT/AU98/00882, International Patent Publication Number: WO 99/23248.
Bhuiyan M, Kim N, Cho Y, Yoon D, Kim KS, Jeon J, Lee J (2009) Identification of SNPs in MYOD gene family and their associations with carcass traits in cattle. Livest Sci 126: 292-297.
Bishop M, Tavakkol A, Threadgill D, Simmen F, Simmen R, Davis M, Womack J (1991) Somatic cell mapping and restriction fragment length polymorphism analysis of bovine insulin-like growth factor I. J Anim Sci 69: 4306-4311.
Botstein D, White R L, Skolnick M, Davis RW (1980). Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32: 314-331.
Burrell D, Moser G, Hetzel J, Mizoguchi Y, Hirano T, Sugimoto Y, Mengersen K (2004) Meta analysis confirms associations of the TG5 thyroglobulin polymorphism with marbling in beef cattle. 29th International conference on animal genetics, Tokyo. pp. 117.
Casas E, Shackelford SD, Keele JW, Stone RT, Kappes SM, Koohmaraie M (2000) Quantitative trait loci affecting growth and carcass composition of cattle segregating alternate forms of myostatin. J Anim Sci 78: 560-569.
Casas E, White SN, Riley DG, Smith TP, Brenneman RA, Olson TA, Johnson DD, Coleman SW, Bennett GL, Chase CC Jr (2005) Assessment of single nucleotide polymorphisms in genes residing on chromosomes 14 and 29 for association with carcass composition traits in Bos indicus cattle. J Anim Sci 83: 13-19.
Cerit H, Dümen E, Sezgin FH (2014) Comparision of DGAT1 K232A polymorphism and its effects on some milk quality parameters in Holstein and Native Black Race cattles. Kafkas Univ Vet Fak 20: 301-305.
Chung E, Kim W (2005) Association of SNP marker in IGF-I and MYF5 candidate genes with growth traits in Korean cattle. Asian-Aust J Anim Sci 18: 1061-1065.
Curi RA, Chardulo LAL, Arrigoni M, Silveira AC, de Oliveira H (2011) Associations between LEP, DGAT1 and FABP4 gene polymorphisms and carcass and meat traits in Nelore and crossbred beef cattle. Livest Sci 135: 244-250.
Curi RA, De Oliveira H, Silveira AC, Lopes C (2005a) Association between IGF-I, IGF-IR and GHRH gene polymorphisms and growth and carcass traits in beef cattle. Livest Prod Sci 94: 159-167.
Curi RA, Oliveira HD, Silveira AC, Lopes C (2005b) Effects of polymorphic microsatellites in the regulatory region of IGF1 and GHR on growth and carcass traits in beef cattle. Anim Genet 36:58-62.
Ekiz B, Ekiz EE, Kocak O, Yalcintan H, Yilmaz A (2012) Effect of pre-slaughter management regarding transportation and time in lairage on certain stress parameters, carcass and meat quality characteristics in Kivircik lambs. Meat Sci 90: 967-976.
Ekiz B, Yilmaz A, Ozcan M, Kaptan C, Hanoglu H, Erdogan I, Yalcintan H (2009) Carcass measurements and meat quality of Turkish Merino, Ramlic, Kivircik, Chios and Imroz lambs raised under an intensive production system. Meat Sci 82: 64-70.
Falconer DS, Mackay TFC (1996) Introduction to quantitative genetics, 4th ed. Pearson Education Ltd. Harlow, England. 280 pp.
Fiems L, De Campeneere S, De Smet S, Van de Voorde G, Vanacker J, Boucqué CV (2000) Relationship between fat depots in carcasses of beef bulls and effect on meat colour and tenderness. Meat Sci 56: 41-47.
Frylinck L, O’Neil A, du Toit E, Strydom PE, Webb EC (2015) The beef tenderness model. S Afr J Anim Sci 45: 234-248.
Gan QF, Zhang LP, Li JY, Hou GY, Li HD, Gao X, Ren HY, Chen JB, Xu SZ (2008) Association analysis of thyroglobulin gene variants with carcass and meat quality traits in beef cattle. J Appl Genet 49: 251-255.
Ge W, Davis M, Hines H, Irvin K, Simmen R (2003) Association of single nucleotide polymorphisms in the growth hormone and growth hormone receptor genes with blood serum insulin-like growth factor I concentration and growth traits in Angus cattle. J Anim Sci 81: 641-648.
Green MR, Sambrook J (2012) Isolation of high-molecular-weight DNA from mammalian cells using proteinase K and phenol, in: Molecular Cloning: A Laboratory Manual, 4, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, USA,
-48.
Grisart B, Coppieters W, Farnir F, Karim L, Ford C, Berzi P, Cambisano N, Mni M, Reid S, Simon P (2002) Positional candidate cloning of a QTL in dairy cattle: identification of a missense mutation in the bovine DGAT1 gene with major effect on milk yield and composition. Genome Res 12: 222-231.
Grunert KG, Bredahl L, Brunso K (2004) Consumer perception of meat quality and implications for product development in the meat sector—a review. Meat Sci 66: 259-272.
Hradecká E, Citek J, Panicke L, Rehout V, Hanusová L (2008) The relation of GH1, GHR and DGAT1 polymorphisms with estimated breeding values for milk production traits of German Holstein sires. Czech J Anim Sci 53: 238-245.
Kisacova J, Kubek A, Melus V, Canakyova Z, Rehout V (2009) Genetic polymorphism of MYF-5 and myostatin in Charolais breed. J Agrobiol 26: 7-11.
Li C, Basarab J, Snelling W, Benkel B, Murdoch B, Hansen C, Moore S (2004) Assessment of positional candidate genes MYF-5 and IGF-1 for growth on bovine chromosome 5 in commercial lines of. J Anim Sci 82: 1-7.
Li X, Ekerljung M, Lundstrom K, Lunden A (2013) Association of polymorphisms at DGAT1, leptin, SCD1, CAPN1 and CAST genes with color, marbling and water holding capacity in meat from beef cattle populations in Sweden. Meat Sci 94: 153-158.
MacNeil M, Grosz M (2002) Genome-wide scans for QTL affecting carcass traits in Hereford× composite double backcross populations. J Anim Sci 80: 2316-2324.
Menezes MPC, Martinez AM, Ribeiro MN, Pimenta Filho EC, Bermejo JVD (2006) Genetic characterization of Brazilian native breeds of goats using 27 markers microsatellites. R Bras Zootec 35: 1336-1341.
Miller J, Thomsen P, Tucker E, Konfortov B (1992) Synteny mapping of the bovine IGHG2, CRC and IGF1 genes. Anim Genet 23: 51-58.
Modika KY, Frylinck L, Moloto KW, Strydom PE, Heinze PH, Webb EC (2015) Visual evaluation of beef tenderness by using surface structural observations and its relationship to meat colour. S Afr J Anim Sci: 45, 255-262.
Moore S, Li C, Basarab J, Snelling W, Kneeland J, Murdoch B, Hansen C, Benkel B (2003) Fine mapping of quantitative trait loci and assessment of positional candidate genes for backfat on bovine chromosome 14 in a commercial line of Bos taurus. J Anim Sci 81: 1919-1925.
Mullen A, Stapleton P, Corcoran D, Hamill R, White A (2006) Understanding meat quality through the application of genomic and proteomic approaches. Meat Sci 74: 3-16.
Nei M, Roychoudhury A (1974) Sampling variances of heterozygosity and genetic distance. Genetics 76: 379-390.
Pannier L, Mullen A, Hamill R, Stapleton P, Sweeney T (2010) Association analysis of single nucleotide polymorphisms in DGAT1, TG and FABP4 genes and intramuscular fat in crossbred Bos taurus cattle. Meat Sci 85: 515-518.
Priolo A, Micol D, Agabriel J (2001) Effects of grass feeding systems on ruminant meat colour and flavour. A review. Anim Res 50: 185-200.
Sañudo C, Macie E, Olleta J, Villarroel M, Panea B, Alberti P (2004) The effects of slaughter weight, breed type and ageing time on beef meat quality using two different texture devices. Meat Sci 66: 925-932.
Shin S, Chung E (2007) Association of SNP marker in the thyroglobulin gene with carcass and meat quality traits in Korean cattle. Asian-Aust. J Anim Sci 20: 172-177.
Siadkowska E, Zwierzchowski L, Oprzadek J, Strzalkowska N, Bag nicka E, Krzyzewski J (2006) Effect of polymorphism in IGF-1 gene on production traits in Polish Holstein-Friesian cattle. Anim Sci Pap Rep 24: 225-237.
Tait RG Jr, Shackelford SD, Wheeler TL, King DA, Keele JW, Casas E, Smith TP, Bennett GL (2014) CAPN1, CAST, and DGAT1 genetic effects on preweaning performance, carcass quality traits, and residual variance of tenderness in a beef cattle population selected for haplotype and allele equalization. J AnimSci 92: 5382-5393.
Thaller G, Kühn C, Winter A, Ewald G, Bellmann O, Wegner J, Zühlke H, Fries R (2003) DGAT1, a new positional and functional candidate gene for intramuscular fat deposition in cattle. Anim Genet 34: 354-357.
Vestergaard M, Oksbjerg N, Henckel P (2000) Influence of feeding intensity, grazing and finishing feeding on muscle fibre characteristics and meat colour of semitendinosus, longissimus dorsi and supraspinatus muscles of young bulls Meat Sci 54: 177-185.
Warner R, Greenwood P, Pethick D, Ferguson D (2010) Genetic and environmental effects on meat quality. Meat Sci 86: 171-183.
Yeh FC, Yang RC, Boyle TB, Ye Z, Mao JX (2000) POPGENE, the user-friendly shareware for population genetic analysis. Molecular biology and biotechnology centre, University of Alberta, Canada.
Zhang R, Chen H, Lei C, Zhang C, Lan X, Zhang Y, Zhang H, Bao B, Niu H, Wang, X (2007) Association between polymorphisms of MSTN and MYF5 genes and growth traits in three Chinese cattle breeds. Asian-Aust J Anim Sci 20: 1798-1804.
Most read articles by the same author(s)