The effects of royal jelly on oxidative stress and toxicity in tissues induced by malathion, an organophosphate insecticide

PDF (English)
DOI: https://doi.org/10.12681/jhvms.20827
L. AKSOY
Department of Chemistry, Faculty of Science and Arts, Afyon Kocatepe University
Y. ALPER
Department of Chemistry, Faculty of Science and Arts, Afyon Kocatepe University
Аннотация

Royal jelly is a bee product frequently used in pharmaceutical, food and cosmetic industries due to its biological activities. The present study aimed to determine the effects of royal jelly on malathion-induced toxicity and biochemical changes. The rats that were used as experimental animals in the study were divided into 6 groups. Control group rats were administered nothing, while carrier chemicals (1% DMSO) were administered to sham group rats. Malathion group (MAL) rats were injected with 0.8 g/kg malathion in DMSO subcutaneously. Saline solution that included 100 mg/kg royal jelly was administered with gavage to the rats in the royal jelly group (RJ). 100 mg/kg royal jelly was administered to RJ+MAL group rats via gavage 1 hour before the injection of 0.8 g/kg malathion. 100 mg/kg royal jelly was administered to MAL+RJ group rats via gavage 1 hour after the injection of 0.8 g/kg malathion. After the experimental process (24 hours), blood samples were taken from the rats in each group under anesthesia (ketamine+xylazine). MDA, NO, GSH, GPx (glutathione peroxidase), CAT, SOD and AChE activities were determined in blood, liver, kidney and brain tissues. It was found that erythrocyte, liver, kidney and brain MDA (malondialdehyde) concentrations in MAL groups were statistically significantly higher when compared to the other groups (p<0.05). It was observed that GSH (glutathione) concentrations increased in the brain, while they decreased in erythrocyte, liver and kidney in the MAL group when compared to the control and sham groups. CAT (catalase) concentration significantly decreased in erythrocyte, liver, kidney and brain tissues in the MAL group when compared to the control and sham groups (p<0.05). SOD (superoxide dismutase) concentration in the MAL group decreased significantly (p<0.05) when compared to other groups, while SOD concentration increased significantly in the therapy and prevention groups (p<0.05) when compared to the others. It was found that serum acetylcholinesterase (AChE) concentration was significantly lower in the MAL group when compared to sham and control groups (p<0.05). Thus, it was concluded that malathion led to lipid peroxidation and oxidative stress in MDA and NO (nitric oxide) levels and toxicity in AChE activities. It was also determined that royal jelly could be effective against oxidative damage and toxicity. The findings suggested that the antioxidant effect of royal jelly could support the treatment of malathion, which is one of the insecticides that contain organophosphate and could lead to oxidative stress. It is considered that the prophylactic characteristics of royal jelly was more effective on malathion toxicity when compared to therapatic properties.

Article Details
  • Раздел
  • Research Articles
Лицензия Creative Commons

Это произведение доступно по лицензии Creative Commons «Attribution-NonCommercial» («Атрибуция — Некоммерческое использование») 4.0 Всемирная.

Authors who publish with this journal agree to the following terms:

· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Скачивания
Данные скачивания пока недоступны.
Библиографические ссылки
Abd El-Monem DD (2011) The Ameliorative Effect of Royal Jelly against Malathion Genotoxicity in Bone Marrow and Liver of Rat. J Am Sci 7(12): 1251-1256.
Aebi H (1974) Catalase. In: Methods of Enzymatic Analysis. 2nd ed, Academic Press, New York: pp. 673-677.
Aker WG, Hu X, Wang P, Hwang HM (2008) Comparing the relative toxicity of malathion and malaoxon in blue catfish Ictalurus furcatus. Environ Toxicol 23(4):548-554.
Aksoy L, Aslan Z (2017) Nephroprotective and antioxidative effects of royal jelly on ethylene glycol induced nephropathy in rats. Vet J Ankara Univ 64 (4): 241-248.
Aksoy L, Hazman O, Buyukben A (2017) Protective characterıstıcs of caffeic acid phenethyl ester (CAPE) on neurodegenerative effects of organophosphate insecticide in rats. Fresen Environ Bull 26 (12):7893-7899.
Alp H, Aytekin İ, Atakişi O, Ogün M (2011) The Effects of Caffeic Acid Phenethyl Ester and Ellagic Acid on Oxidative Stress Created by Acute Malathion Toxicity in Rat. Atatürk Uni Vet Sci J 6(2): 117-124.
Altuntas I, Delibas N, Doguc DK, Ozmen S, Gultekin F (2003) Role of reactive oxygen species in organophosphate insecticide phosalone toxicity in erythrocytes in vitro. Toxicol In Vitro 17(2):153-157.
Barón V, Muriel P (1999) Role of glutathione, lipid peroxidation and antioxidants on acute bile-duct obstruction in the rat. Biochim Biophys Acta 18: 173-180.
Beutler E, Dubon O, Kelly BM (1963) Improved method for the determination of blood glutathione. J Lab Clin Med 61: 882-888.
Bincoletto C, Eberlin S, Figueiredo CAV, Luengo MB, Queiroz MLS (2005) Effects produced by Royal Jelly on haematopoiesis: relation with host resistance against Ehrlich ascites tumour challenge. Int Immunopharmacol 5: 679-688.
Bucekova M, Sojka M, Valachova I, Martinotti S, Ranzato E, Szep Z, Majtan V, Klaudiny, Majtan Juraj (2017) Bee-derived antibacterial peptide, defensin-1, promotes wound re-epithelialisation in vitro and in vivo. Scientific Reports 7 (7340) : 1-13.
Cheeseman KH, Slater TF (1993) An introduction to free radical biochemistry. Br Med Bull 49 (3): 481-493.
Choudhary N, Goyal R, Joshi SC (2008) Effect of malathion on reproductive system of male rats, Journal of Environmental Biology 29(2):259-262.
Cıhan YB, Ozturk A, Gokalp SS (2013) Protective Role of Royal Jelly Against RadiationInduced Oxidative Stress in Rats. Int J Hematol Oncol 23: 79-87.
Costa LG, Giordano G, Guizzetti M, Vitalone A (2008) Neurotoxicity of pesticides: a brief review. Front Biosci 13: 1240-1249.
Culotta VC, Yang M, O’Halloran TV (2006) Activation of superoxide dismutases: Putting the metal to the pedal. Biochim Biophys Acta 1763(7): 747-758.
Fontana R, Mendes MA, De Souza BM, Konno K, Cesar LMM, Malaspına O, Palma MS. Jelleines (2004) A family of antimicrobial peptides from the royal jelly of honeybees (Apis Mellifera). Peptides 25(6):919-928.
Gaweł S, Wardas M, Niedworok E, Wardas P (2004) Malondialdehyde (MDA) as a lipid peroxidation marker. Wiad Lek 57: 453-455.
Gechev T, Gadjev I, Van Breusegem F, Inzé D, Dukiandjiev S, Toneva V, Minkov I (2002) Hydrogen peroxide protects tobacco from oxidative stress by inducing a set of antioxidant enzymes. Cell Mol Life Sci 59(4):708-714.
Gutteridge JM, Mitchell J (1999) Redox imbalance in the critically ill. Br Med Bull 55( 1): 49-75.
Heidrick ML, Hendricks LC, Cook DE (1984) Effect of dietary 2-mercaptoethanol on the life span, immune system, tumor incidence and lipid peroxidation damage in spleen lymphocytes of aging BC3F1 mice. Mech Ageing Dev 27: 341-358.
Jain SK, McVie R, Duett J, Herbst JJ. (1989) Erythrocyte membrane lipid peroxidation and glycosylated hemoglobin in diabetes. Diabetes 38:1539-1543.
Jeyaratnam J (1990) Acute pesticide poisoning: a major global health problem. World Health Stat Q 43(3): 139-144.John S, Kale M, Rathore N, Bhatnagar D (2001) Protective effect of vitamin E in dimethoate and malathion induced oxidative stress in rat erythrocytes. J Nutr Biochem 12: 500-504.
Kalender S, Uzun FG, Durak D, Demir F, Kalender Y (2010) Malathion induced hepatotoxicity in rats: The effects of vitamins C and E. Food Chem Toxicol 48: 633-638.
Khazaei M, Ansarian A, Ghanbari E (2018) New Findings on Biological Actions and Clinical Applications of Royal Jelly: A Review. J Diet Suppl 15(5): 757-775.
Kim J, Kim Y, Yun H, Park H, Kim SY, Lee KG, Han SM, Cho Y (2010) Royal jelly enhances migration of human dermal fibroblasts and alters the levels of cholesterol and sphinganine in an in vitro wound healing model. Nutr Res Pract 4(5): 362-368.
Majtan J, Kumar P, Majtan T, Walls AF, Klaudıny J (2010) Effect of honey and its major royal jelly protein 1 on cytokine and mmp-9 mrna transcripts in human keratinocytes. Exp Dermatol 19(8): 73-79.
Miranda KM, Espey MG, Wink DA (2001) A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide 5: 62-71.
Moore PD, Patlolla AK, Tchounwou PB (2011) Cytogenetic evaluation of malathion-induced toxicity in Sprague-Dawley rats. Mutat Res 725 (1-2): 78-82.
Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animals and tissues by thiobarbituric acid reaction. Anal Biochem 95: 351-358.
Okamoto I, Taniguchi Y, Kunata T, Kohno K, Iwaki K, Ikeda M, Kurimoto M (2003) Major royal jelly protein 3 modulates immune responses in vitro and in vivo. Life Sci 5; 73(16): 2029-2045.
Possamai FP, Fortunato JJ, Feier G, Agostinho FR, Quevedo J, Filho DW, Dal-Pizzol F (2007) Oxidative stress after acute and sub-chronic malathion intoxication in Wistar rats. Environ Toxicol Pharmacol 23; 198-204.
Salem MM, El- Rasheid HGA, Mahmoud AN (2015) Therapeutic effects of curcumin and royal jelly as natural antioxidants on some biochemical parameters in hepatotoxicity induced by carbon tetrachloride (CCl4) in male albino rats. Int J Adv Res 3 (11): 520-535.
Sodhi S, Sharma A, Brar APS, Brar RS (2008) Effect of a tocopherol and selenium on antioxidant status, lipid peroxidation and hepatopathy induced by malathion in chicks. Pestic Biochem Physiol 90: 82-86.
Stuehr DJ (2004) Enzymes of the L-arginine to nitric oxide pathway. J Nutr 134: 2748-2751.
Tamura T, Fujii A, Kumoyama N (1987) Antitümor effect of royal jelly. Folia Pharmacol Japon 89(2):73-80.
Supabphol R, Supabphol A (2013) Cytoprotective Potential of Royal Jelly on Human Umbilical Vein Endothelial Cells against Nicotine Toxicity via Catalase. European J Med Plants 3(1): 88-98.
Timur S, Önal S, Karabay Ü, Sayım F, Zihnioğlu F (2003) In vivo Effects of Malathion on Glutathione-S-Transferase and Acetylcholinesterase Activities in Various Tissues of Neonatal Rats. Turk J Zool 27: 247-252.
Tós-Luty S, Obuchowska-Przebirowska D, Latuszyńska J, Tokarska-Rodak M, Haratym-Maj A (2003) Dermal and oral toxicity of malathion in rats. Ann Agric Environ Med 10(1):101-116.
Wankhade V, Malu AR, Pawar SP (2009)Effect of malathion on liver ache activity of mice. Biol Med 1(2): 122-126.
Worek F, Bäcker M, Thiermann H, Szinicz L, Mast U, Klimmek R, Eyer P (1997) Reappraisal of indications and limitations of oxime therapy in organophosphate poisoning. Hum Exp Toxicol 16(8): 466-472.