Ameliorative effects of L-carnitine on florfenicol-induced hepatotoxicity in broilers


Опубликован: Apr 29, 2022
Nourhan A. Haggag
https://orcid.org/0000-0001-9861-9693
Mustafa A. Aziz
Abu Elnasr A. Zahra
Soad S. Belih
Hazim Omar Khalifa
https://orcid.org/0000-0001-9861-9693
Аннотация

L-carnitine is a non-essential amino acid derivative naturally occurring and widely distributed in nature. It received a growing interest in its potential uses as a medicinal agent possess protective effects that postulated to be related to its antioxidant action. This study was aimed to evaluate the ameliorative role of L-carnitine on florfenicol induced hepatic toxicity in broilers. A total of 150 broiler chicks were grouped into 6 groups each of 25 chicks. Group one was kept as a control group, while group two and three were treated with florfenicol and L-carnitine, respectively. Group 4 was pre-treated with L-carnitine for three days before florfenicol administration. Groups five and sex were cotreated with L-carnitine and florfenicol and post-treated with L-carnitine for three days after florfenicol administration, respectively. The biochemical analysis, liver indices, antioxidant profile, and histopathological examination were performed to evaluate its ameliorative effects. Results emphasized that florfenicol induced hepatic toxicity in broilers and L-carnitine can ameliorate its action when its usage preceded the florfenicol or when they were used together which reflected by an enhancement in liver indices, antioxidant profile, and histopathological findings. As far as we know this the first study confirming the ameliorative potency of L-carnitine on florfenicol-induced hepatotoxicity.

Article Details
  • Раздел
  • Research Articles
Скачивания
Данные скачивания пока недоступны.
Библиографические ссылки
Abu-El-Zahab HS, Hamza RZ, Montaser MM, El-Mahdi MM, Al-Harthi WA (2019) Antioxidant antiapoptotic antigenotoxic and hepatic ameliorative effects of L-carnitine and selenium on cadmium-induced hepatotoxicity and alterations in liver cell structure in male mice. Ecotoxicol Environ Saf 173:419–428.‏
Adabi SG, Cooper RG, Ceylan N, Corduk M (2011) L-carnitine and its functional effects in poultry nutrition. J Poult Sci 67:277–296
Agarwal A, Said TM (2004) L-Carnitines and male infertility. Reprod Biomed Online 8:376–384.
Ahmed SA (2016) Hepatoprotective effects of L-carnitine against cyclosporine A-induced liver injury in white albino rats; a newly proposed mechanism of action. IJRPP 5:215–225.
Amacher DE (1998) Serum transaminase elevations as indicators of hepatic injury following the administration of drugs. Regul Toxicol Pharm 27:119–130.
Augustyniak A, Skrzydlewska E (2009) L-Carnitine in the lipid and protein protection against ethanol-induced oxidative stress. Alcohol 43:217−223.
Bancroft JD, Gamble M (2008) Theory and Practice of Histopathological Techniques.6th edition Churchill Living stone. New York London and Madrid.
Baumgartner M, Blum R (1997) Feeds without animal meal: Is adequate L-carnitine provision still safeguarded? In Baumgartner, M (ed.) LONZA Ltd, Muenchensteinerstrasse 38, CH-4002 Basel, Switserland.
Bayraktar N, Pasaoglu H, Pasaoglu A, Kaymaz M, Biberoglu G, Kulaksizoglu S (2008) The relationship between carnitine levels and lipid peroxidation in glial brain tumors. Turk J Med Sci 38:293–299.
Belfield A, Goldberg DM (1971) Revised assay for serum phenyl phosphatase activity using 4-amino-antipyrine enzyme. Enzyme 12:561–573.
Çekın AH, Gür G, Türkoğlu S, Aldemır D, Yilmaz U, Gürsoy M, Boyacioğlu S (2013) The protective effect of L-carnitine on hepatic ischemia-reperfusion injury in rats. Turk J Gastrtrol 24 :51-56.
Del Rio D, Stewart A, Pellegrini N (2005) A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress. Nutr Metab Cardiovasc Dis 15:316–328.
Dokmeci D, Akpolat M, Aydogdu N, Uzal C, Doganay L, Turan FN (2006) The protective effect of L-carnitine on ionizing radiation-induced free oxygen radicals. Scand J Lab Anim Sci 33: 75–83.
Dowling PM (2013) Chloramphenicol thiamphenicol and florfenicol. Antimicrobial therapy in veterinary medicine. pp 241-48.
El-Demerdash F, Yousef M, Radwan F (2009) Ameliorating effect of curcumin on sodium arsenite-induced oxidative damage and lipid peroxidation in different rat organs. Food Chem Toxicol 47:249–254.
Er A, Dik B (2014) The effects of florfenicol on the values of serum tumor necrosis factor-and other biochemical markers in lipopolysaccharide-induced endotoxemia in brown trout. Mediators of inflammation. pp 1–5.
Hassanin O, Abdallah F, Awad A (2014) Effects of florfenicol on the immune responses and the interferon-inducible genes in broiler chickens under the impact of E. coli infection. Vet Res Commun 38:51–58.
Jahovic N, Şener G, Çevik H, Ersoy Y, Arbak S Yeğen BÇ (2004) Amelioration of methotrexate-induced enteritis by melatonin in rats. Cell Biochem Funct: Cellular biochemistry and its modulation by active agents or disease 22: 169–178.
Jendrassik L, Grof P (1938) Quantitative determination of total and direct bilirubin in serum and plasma. Biochem Z 297:81–89.
Jiao K, Ma J, Yuan Y, Liu Z, Ma X, Cen H (2009) The impact of different dose florfenicol on blood biochemical indexes in pigs and antibody against classical swine fever virus. Chin J Vet Med 29:1586-1589.
KalaIselvi T, Panneerselvam C (1998) Effect of L-carnitine on the status of lipid peroxidation and antioxidants in aging rats. J Nutr Biochem 9:575–581.
Khalil S, Hamed E, Hassanin O (2012) Residue withdrawal of florfenicol from the serum and edible tissues of broiler chickens. J Am Sci 8:514–524.
Marwa AE, Bakry HH, El-Ahawarby RM, Abou Salem ME, Elham A (2013) Residual studies of florfenicol in broiler chicken. BVMJ 24:209–217.
Mehdi Y, Létourneau-Montminy MP, Gaucher ML, Chorfi Y, Suresh G, Rouissi T, Brar SK, Côté C, Ramirez AA, Godbout S (2018) Use of antibiotics in broiler production: Global impacts and alternatives. Ani Nutr 4:170–178.
Mousah HA, Hayder B, Sahib BH, Kadhum HH (2016) Protective effect of L-carnitine atorvastatin and vitamin A on acetaminophen induced hepatotoxicity in rats. Int. J. Pharm Sci Rev Res 36:21–27.
Murrary RL, Kaplan LA (1984) Creatinine In: Clinical Chemistery Theory Analysis and Correlation Kaplan L.A. and A. J. Pesce (Eds). CV Mosby Co. St. Louis. PP: 1247–1253.
Nouboukpo KE, Tana K, Kamers B, Everaert N, Willemsen H (2010) Effect of In vivo administration of L-carnitine on hatching events and juvenile performance of layer type chicken. Int J Poult Sci 9:980–983.
Ren X, Pan L, Wang L (2014) Effect of florfenicol on selected parameters of immune and antioxidant systems and damage indexes of juvenile Litopenaeusvannamei following oral administration. Aquaculture 432:106–113.
Sakeran MI, Zidan N, Rehman H, Aziz AT, Saggu S (2014) Abrogation by Trifoliumalexandrinum root extract on hepatotoxicity induced by acetaminophen in rats. Redox Rep 19:26-33.
Sams RA (1994) Florfenicol Chemistry and metabolism of a novel broad spectrum antibiotic. In Proceedings of the XVIII World Buiatrics Congress Bologna Italy. PP: 13-17.
Surai PF (2015) Antioxidant action of carnitine: molecular mechanisms and practical applications. EC Vet Sci 2:66–84.
Van Boeckel TP, Brower C, Gilbert M, Grenfell BT, Levin SA, Robinson TP, Teillant A, Laxminarayan R (2015) Global trends in antimicrobial use in food animals. PNAS 112:5649-2654.
Wei CF, Shien JH, Chang SK, Chou CC (2016) Florfenicol as a modulator enhancing antimicrobial activity: example using combination with thiamphenicol against Pasteurella multocida. Front Microbiol 7:389.
Wu M, Xu H, Shen Y, Qiu W, Yang M (2011) Oxidative stress in zebra fish embryos induced by short term exposure to bisphenol A nonylphenol and their mixture. Environ Toxicol Chem 30: 2335–2341.
Yuxuan Z, Peiyong G, Yanmei W, Xiaoyan Z, Meixian W, Simin Y, Haitao S (2019) Evaluation of the subtle effects and oxidative stress response of chloramphenicol thiamphenicol and florfenicol in Daphnia magna. Environ Toxicol Chem 38: 575–584.