In vitro anticoccidial activity of Vitis vinifera extract on oocysts of different Eimeria species of Broiler Chicken


R.Z. ABBAS
A. ABBAS
Z. IQBAL
M.A. RAZA
K. HUSSAIN
T. AHMED
M.U. SHAFI
Аннотация

In the current experiment, the in vitro anticoccidial effect of Vitis venifera (grape seed) extract was evaluated. For this purpose, an in vitro sporulation inhbition assay was used. Collected oocysts of four Eimeria species (E. tenella, E. necatrix, E. brunetti and E. mitis) were exposed to six different concentrations (w/v) of Vitis vinifera extract (VVE) in 10% Dimethylsulphoxide solution (DMSO), while Dimethylsulphoxide (DMSO) and Potassium dichromate solution (K2Cr2O7) served as control groups. The results of the present study revealed that V. vinifera extract showed inhibitory effect on sporulation (%) and damage (%) of Eimeria oocysts in a dose dependent manner as compared to both control groups. V. vinifera extract also damaged the morhology of oocysts in terms of shape, size and number of sporocysts.

Article Details
  • Раздел
  • Research Articles
Скачивания
Данные скачивания пока недоступны.
Библиографические ссылки
Abbas A, Z Iqbal, Abbas RZ, Khan MK, Khan JA (2017) Immunomodulatory activity of Pinus radiata extract against coccidiosis in broiler chicken. Pak Vet J 37: 145-149.
Abbas A, Abbas RZ, Khan MK, Raza MA, Mahmood MS, Saleemi MK, Hussain T, Khan JA, Sindhu ZD (2019a) Anticoccidial Effects of Trachyspermum ammi (Ajwain) in broiler chickens. Pak Vet J 39:301-304.
Abbas A, Abbas RZ, Raza MA, Khan MK, Saleemi MK, Saeed Z (2019b) In vitro anticoccidial activity of Trachyspermum ammi (Ajwain) extract on oocysts of Eimeria species of Chicken. Adv Life Sci 7: 44-47.
Alzahrani F, Al-Shaebi EM, Dkhil MA, Al-Quraishy S (2016) In vivo anti-Eimeria and in vitro anthelmintic activity of Ziziphusspina-christi leaf extracts fares, Pak J Zool 48: 409-413.
Abbas A, Iqbal Z, Abbas RZ, Khan MK, Khan JA (2015) In-vitro anticoccidial potentaial of Sacharrumofficiarum extract against Eimeria Oocysts. Bollatinoam. Caribe Plantas Med Aromát14: 456-46.
Bachaya HA, Abbas RZ, Raza MAM, Iqbal Z, Rehman TU, Baber W, Hussain R (2015). Existence of coccidiosis and associated risk factors in broiler chickens in Southern Punjab, Pakistan. PakVet J 35: 81-84.
Bakr AF, Abdelgayed SS, EL-Tawil OS, Bakeer AM (2019) Assessment of ginger extract and ginger nanoparticles protective activity against acetaminophen-induced hepatotoxicity and nephrotoxicity in rats. Pak Vet J 39: 479-486.
Farieha, Jahan N, Rehman KU and Ali S (2019) Effect of herbal mixture as Angiotensin converting enzyme inhibitor in Angiotensin-II dependent hypertension. Pak Vet J 39: 25-30.
Gadelhaq SM, Arafa WM, Abolhadid SM (2018) In vitro activity of natural and chemical products on sporulation of Eimeria species oocysts of chickens. Vet Parasitol 251:12-6.
Kara K, Guclu BK, Baytok E, Senturk M (2016) Effects of grape pomace supplementation to laying hen diet on performance, egg quality, egg lipid peroxidation and some biochemical parameters. J Appl Animal Res 44: 303-10.
Kasapidou E, Soddidou EN, Zdragas A, Papadaki C, Vafeas G, Mtlianga P (2016) Effect of grape pomace supplementation on broiler meat quality characteristics. Eur Poult Sci 80:135-42.
Mujahid H, Hashmi AS, Khan MZ, Tayyab M, Shezad W (2019) Protective effect of yeast sludge and whey powder against ochratoxicosis in broiler chicks. Pak Vet J 39: 588-592.
Masood S, Abbas RZ, Iqbal Z, Mansoor MK, Sindhu ZD, Zia MA, Khan JA (2013) Role of natural antioxidants for the control of Coccidiosis in Poultry. Pak Vet J 33: 401-407.
Molan AL, Thomas DV (2007) Green tea water extracts inhibit the sporulation of Eimeria oocysts in vitro. Advancing Poultry Production –Proceedings of the Massey Technical Update Conference. 9: 89–97.
Molan AL, Zhuojian L, De S (2009) Effect of pine bark (Pinusradiata) extracts on sporulation of coccidian oocysts. Folia Parasitol 56: 1–5.
Nweze NE, Obiwulu IS (2009) Anticoccidial effects of Ageratum conyzoides. J Ethnopharmacol 122: 6–9.
Remmal A, Sanaa A, Latifa B, Fouzia C, Najat C (2013) Oocysticidal Effect of Essential Oil Components against Chicken Eimeria Oocysts. Int J Vet Med 2: 133-139.
Ryley JF, Meade R, burst JH, Robinson TE (1976) Methods in Coccidiosis research: Separation of oocysts from faeces. J Parasitol 73: 311-326.
Sarwar N, Mehmood A, Sheraz A, Noman M (2019) Protective effect of probiotics in combination with vaccination on antibody response, biochemical and hematological indices in broilers. Pak Vet J 39: 451-454.
You MJ (2014) Suppression of Eimeria tenella sporulation by disinfectants. Korean J Parasitol 52: 435–438.
Wang MI, Suo X, , Gu JH, Zhang WW, Fang Q, Wang X (2008) Influence of grape seed proanthocyanidin extract in broiler chickens: effect on chicken coccidiosis and antioxidant status. Poult Sci 87:2273-2280.
Zaman MA, Iqbal Z, Abbas RZ, Khan MN (2012) Anticoccidial activity of herbal complex in broiler chickens challenged with Eimeria tenella. Parasitology 139: 237-243.
Zhang QH, Nie FH, Wang XN, Lin HY, Wang HCR, Gooneratne R, Chen JJ (2018) Effect of flavonoids from Eupatorium odoratum L. on immunoglobulins and cecal microflora in broilers. Pak Vet J 38: 296-300.
Zhang K, Li X, Na C, Abbas A, Abbas RZ, Zaman MA (2020) Anticoccidial effects of Camellia sinensis (green tea) extract and its effect on Blood and Serum chemistry of broiler chickens. PakVet J 40: 77-80.