Molecular and histopathologic investigation of Pestivirus, Chlamydophila abortus and Listeria monocytogenes infections in aborted sheep foetuses


Опубликован: Apr 29, 2022
Özgür Kanat
https://orcid.org/0000-0002-9747-5496
Аннотация
The aims of this study was to investigate the presence of pestiviruses, Chlamydophila abortus (C. abortus) and Listeria monocytogenes (L. monocytogenes) and histopathological findings caused by these agents in aborted sheep foetuses. A total of 52 aborted sheep foetuses, aged between 1 to 5 months of gestation, were collected from Konya province in Turkey. Molecular techniques were used for detection pestivirus RNA, C. abortus and L. monocytogenes DNA in investigated samples. Pestivirus RNA was detected in 6 (11.5%) of the 52 aborted sheep foetuses whereas C. abortus DNA was determined in 8 (15.4%) foetuses. However, L. monocytogenes DNA was not detected in investigated samples. The significant histopathological findings were hypomyelination, degeneration and necrosis of neurons in the brain, interstitial pneumonia, mononuclear infiltrations in the liver, hyperemia and proximal tubule degeneration in the cortex of kidney in pestivirus positive samples and multifocal purulent-necrotic foci, diffuse neutrophils and mononuclear infiltrations in the liver and spleen, hyperaemia, bleeding, intramyelinic and perivascular oedema, gliosis, neuronophagia in the brain, hyperemia, proximal tubule degeneration and neutrophil granulocyte infiltration in the cortex of kidney were observed in C. abortus positive samples. The results of the study show that C. abortus and pestivirus infections play an important role in abortion in sheep.
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Библиографические ссылки
Agerholm JS, Aalbaek B, Fog-Larsen AM, Boye M, Holm E, Jensen TK, Lindhardt T, Larsen LE, Buxton D (2006) Veterinary and medical aspects of abortion in Danish sheep. APMIS: 114, 146-152. doi: 10.1111/j.1600-0463.2006.apm_362.x
Arif ED, Saeed NM, Rachid SK (2020) Isolation and Identification of Chlamydia abortus from Aborted Ewes in Sulaimani Province, Northern Iraq. Pol J Microbiol 69: 1-7. doi: 10.33073/pjm-2020-009
Bulut H, Sozdutmaz I, Pestil Z, Abayli H, Sait A, Cevik A (2018) High Prevalence of Bovine Viral Diarrhea Virus-1 in Sheep Abortion Samples with Pestivirus Infection in Turkey. Pak Vet J 38: 71-75. doi: 10.29261/pakvetj/2018.014
Buxton D, Anderson IE, Longbottom D, Livingstone M, Wattegedera S, Entrican G (2002) Ovine chlamydial abortion: characterization of the inflammatory immune response in placental tissues. J Comp Pathol 127: 133-141.
Constable PD, Hinchcliff KW, Done SH, Grünberg W (2017) Diseases of the Nervous System. In: Veterinary Medicine: A Textbook of The Diseases of Cattle, Horses, Sheep, Pigs, and Goats. 11th edition. St. Louis, Missouri: Elsevier.
Çokçalışkan C (2002) Pestivirus infections of pregnant sheep and their fetuses. PhD thesis, Ankara University Health Science Institute, Ankara, Turkey.
Feknous N, Hanon JB, Tignon M, Khaled H, Bouyoucef A, Cay B (2018) Seroprevalence of border disease virus and other pestiviruses in sheep in Algeria and associated risk factors. BMC Vet Res 14: 339. doi: 10.1186/s12917-018-1666-y
Gökçe HI, Kacar C, Genç O, Sözmen M (2007) Seroprevalence of Chlamydophila abortus in aborting ewes and dairy cattle in the North-East part of Turkey. Bull Vet Inst Pulawy 51: 9-13.
Heidari S, Derakhshandeh A, Firouzi R, Ansari-Lari M, Masoudian M, Eraghi V (2018) Molecular detection of Chlamydophila abortus, Coxiella burnetii, and Mycoplasma agalactiae in small ruminants' aborted fetuses in southern Iran. Trop Anim Health Prod 50: 779-785. doi: 10.1007/s11250-017-1494-2
Kalender H, Kiliç A, Eröksüz H, Muz A, Kılınç Ü, Taşdemir B (2013) Identification of Chlamydophila abortus infection in aborting ewes and goats in Eastern Turkey. Rev Méd Vét 164: 295-301.
Karaca M, Babür C, Çelebi B, Akkan HA, Tütüncü M, Keleş İ, Uslu BA, Kiliç S (2007) Investigation on the seroprevalence of Toxoplasmosis, Listeriosis and Brucellosis in goats living in the region of Van, Turkey. YYÜ Vet Fak Derg 18: 45-49.
Kennerman E, Erdogan HM, Şenturk S, Golcu E (2000) Serological diagnosis of listeriosis by ELISA in sheep in Bursa region. Turkish J Vet Surg 6: 15-19.
Krametter-Froetscher R, Mason N, Roetzel J, Benetka V, Bago Z, Moestl K, Baumgartner W (2010) Effects of Border disease virus (genotype 3) naturally transmitted by persistently infected sheep to pregnant heifers and their progeny. Vet Med 55: 145-153.
Livingstone M, Wheelhouse N, Ensor H, Rocchi M, Maley S, Aitchison K, Wattegedera S, Wilson K, Sait M, Siarkou V, Vretou E, Entrican G, Dagleish M, Longbottom D (2017) Pathogenic outcome following experimental infection of sheep with Chlamydia abortus variant strains LLG and POS. PLoS One 12: e0177653. doi: 10.1371/journal.pone.0177653
Longbottom D, Livingstone M, Maley S, van der Zon A, Rocchi M, Wilson K, Wheelhouse N, Dagleish M, Aitchison K, Wattegedera S, Nath M, Entrican G, Buxton D (2013) Intranasal infection with Chlamydia abortus induces dose-dependent latency and abortion in sheep. PLoS One 8: e57950. doi: 10.1371/journal.pone.0057950
Menzies PA (2007) Abortion in sheep: diagnosis and control. In: Current therapy in large animal theriogenology. 2nd edition. Elsevier, St. Louis: Elsevier, St. Louis.
Miller MA, Turk JR, Nelson SL, Van der Lek AP, Solorzano R, Fales WH, Morehouse LG, Gosser HS (1990) Chlamydial infection in aborted and stillborn lambs. J Vet Diagn Invest 2: 55-58. doi: 10.1177/104063879000200110
Navarro JA, Garcia de la Fuente JN, Sanchez J, Martinez CM, Buendia AJ, Gutierrez-Martin CB, Rodriguez-Ferri EF, Ortega N, Salinas J (2004) Kinetics of infection and effects on the placenta of Chlamydophila abortus in experimentally infected pregnant ewes. Vet Pathol 41: 498-505. doi: 10.1354/vp.41-5-498
Oguzoglu TC, Tan MT, Toplu N, Demir AB, Bilge-Dagalp S, Karaoglu T, Ozkul A, Alkan F, Burgu I, Haas L, Greiser-Wilke I (2009) Border disease virus (BDV) infections of small ruminants in Turkey: a new BDV subgroup? Vet Microbiol 135: 374-379. doi: 10.1016/j.vetmic.2008.09.085
Pugh DG, Baird AN, Edmondson M, Passler T (2020) Theriogenology of sheep goats and cervids. In: Sheep, Goat and Cervid Medicine. 3rd edition. 141-208.
Rossmanith P, Krassnig M, Wagner M, Hein I (2006) Detection of Listeria monocytogenes in food using a combined enrichment/real-time PCR method targeting the prfA gene. Res Microbiol 157: 763-771. doi: 10.1016/j.resmic.2006.03.003
Schlafer DH, Foster RA (2016) Female genital system. In: Jubb, kennedy, and palmer’s pathology of domestic animals. Sixth edition. Elsevier.
Shoukat S, Mailk SVS, Rawool DB, Kumar A, Kumar S, Shrivastava S, Barbuddhe SB, Das DP, Das S (2014) A Study on Detection of pathogenic Listeria monocytogenes in ovine’s of Kashmir region having abortion or history of abortion. Proc Natl Acad Sci India, Sect B Biol Sci 84: 311.
Sozzi E, Lavazza A, Gaffuri A, Bencetti FC, Prosperi A, Lelli D, Chiapponi C, Moreno A (2019) Isolation and full-length sequence analysis of a Pestivirus from aborted lamb fetuses in Italy. Viruses-Basel 11: 744. doi:10.3390/v11080744
Şevik M (2018) The role of Pestiviruses (BDV and BVDV) in ruminant abortion cases in the Afyonkarahisar province. Kocatepe Vet J 11: 238-244.
Thiele D, Wittenbrink MM, Fischer D, Krauss H (1992) Evaluation of the polymerase chain reaction (PCR) for detection of Chlamydia psittaci in abortion material from ewes. Zentralbl Bakteriol 277: 446-453.
TÜİK (2019) TÜİK. Canlı Hayvan Sayısı: Türkiye İstatistik Kurumu, Ankara, Turkey. Retrieved from https://biruni.tuik.gov.tr/medas/?kn=101&locale=tr
Ural K, Ural DA, Celebi B, Haydardedeoğlu AE, Babur C, Baritci İ, Kiliç S (2009) Seroprevalence of Listeriosis, Toxoplasmosis and Brucellosis in Saanen X Kilis and Angora goats in Ankara. FÜ Sağ Bil Vet Derg 23: 79-82.
Ural ZE, Erol N (2017) Aydın ve İzmir illerindeki koyun ve keçilerde Pestivirus enfeksiyonunun serolojik ve virolojik olarak araştırılması. Harran Üniv Vet Fak Derg 6: 63-68.
Vilcek S, Herring AJ, Herring JA, Nettleton PF, Lowings JP, Paton DJ (1994) Pestiviruses isolated from pigs, cattle and sheep can be allocated into at least three genogroups using polymerase chain reaction and restriction endonuclease analysis. Arch Virol 136: 309-323.
Wagner M, Melzner D, Bago Z, Winter P, Egerbacher M, Schilcher F, Zangana A, Schoder D (2005) Outbreak of clinical listeriosis in sheep: evaluation from possible contamination routes from feed to raw produce and humans. J Vet Med B Infect Dis Vet Public Health 52: 278-283. doi: 10.1111/j.1439-0450.2005.00866.x
Wang Y, Wang Y, Ye CY 2(018) Endonuclease restriction-mediated real-time PCR for simultaneous detection of Listeria monocytogenes and Listeria ivanovii. Anal Methods 10: 1339-1345. doi: 10.1039/c7ay02667f