Detection of Mycobacterium avium subsp. paratuberculosis in liver of slaughtered cattle, sheep and goats by PCR and culture

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DOI: https://doi.org/10.12681/jhvms.26751
M ZAREI
Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
S TAJBAKHSH
Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
M GHORBANPOUR
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
A YOUSEFVAND
Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
S RAHIMI
Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
M SHAHPOURI
Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
A AMERI
Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
N MOSAVARI
Department of Bacteriology, Razi Vaccine and Serum Research Institute, Hessarak, Karaj, Iran
Аннотация

The presence of Mycobacterium avium subsp. paratuberculosis (MAP) in tissues other than intestines and associated lymph nodes is a potential public health concern. Therefore, the presence of MAP in the liver of slaughtered cattle, sheep and goats with unknown Johne’s disease status was evaluated. Liver samples were collected randomly from 470 mature animals including 200 cattle, 135 sheep and 135 goats. The existence of MAP DNA from obtained liver samples was determined using PCR, thereafter PCR-confirmed samples were cultured. Based on liver PCR results, IS900 genes were detected in 11/200 (5.5%), 3/135 (2.2%), 2/135 (1.48%) liver samples of cattle, sheep and goats, respectively. In cattle, sheep and goats, 6 (54.5%), 1 (33.3%) and 1 (50%) PCR-positive samples showed positive results in culture, respectively. Our results revealed that MAP can be detected and cultured from the liver of slaughtered cattle, sheep and goats with unknown Johne’s disease status. Hence, given concerns about a potential zoonotic role for MAP, there is a crucial need to detect animals with MAP disseminated infection in the liver before slaughter.

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Библиографические ссылки
Alonso-Hearn M, Molina E, Geijo M, Vazquez P, Sevilla I, Garrido JM, Juste RA (2009) Isolation of Mycobacteriumavium subsp paratuberculosis from muscle tissue of naturally infected cattle. Foodborne Pathog Dis 6:513-518.
Antognoli MC, Garry FB, Hirst HL, Lombard JE, Dennis MM, Gould DH, Salman MD (2008) Characterization of Mycobacterium avium subspecies paratuberculosis disseminated infection in dairy cattle and its association with antemortem test results. Vet. Microbiol 127:300-308.
Behr MA, Kapur V (2008) The evidence for Mycobacterium paratuberculosis in Crohn’s disease. Curr Opin Gastroenterol, 24:17-21.
Bower KL, Begg DJ, Whittington RJ (2011) Culture of Mycobacterium avium subspecies paratuberculosis (MAP) from blood and extra-intestinal tissues in experimentally infected sheep. Vet. Microbiol 147:127-132.
Bull TJ, Mcminn EJ, Sidi-Boumedine K, Skull A, Durkin D, Neild P, Rhodes G, Pickup R, Hermon-Taylor J (2003) Detection and verification of Mycobacterium avium subsp. paratuberculosis in fresh ileocolonic mucosal biopsy specimens fromindividuals with and without Crohn’s disease. J Clin Microbiol 41:2915-2923.
de Juan L, Alvarez J, Romero B, Bezos J, Castellanos E, Aranaz A, Mateos A, Dominguez L (2006) Comparison of four different culture media for isolation and growth of type II and type I/III Mycobacterium avium subsp. paratuberculosis strains isolated from cattle and goats. Appl Environ Microbiol 72(9):5927-5932.
Dukkipati VSR, Ridler AL, Thompson KG, Buddle BM, Hedgespeth BA, Price-Carterb M, Begg DJ, Whittington RJ, Gicquel B et al (2016) Experimental infection of New Zealand Merino sheep with a suspension of Mycobacterium avium subspecies paratuberculosis (Map) strain Telford: Kinetics of the immune response, histopathology and Map culture. Vet. Microbio 195:136-143.
Ellingson JL, Anderson JL, Koziczkowski JJ, Radcliff RP, Sloan SJ, Allen SE, Sullivan NM (2005) Detection of viable Mycobacterium avium subsp. paratuberculosis in retail pasteurized whole milk by two culture methods and PCR. J Food Prot 68(5):966-972.
Ellingson JL, Bolin CA, Stabel JR (1998) Identification of a gene unique to Mycobacterium avium subspecies paratuberculosis and application to diagnosis of paratuberculosis. Mol Cell Probes 12:133-142.
Gao A, Mutharia L, Chen S, Rahn K, Odumeru J (2002) Effect of pasteurization on survival of Mycobacterium paratuberculosis in milk. J Dairy Sci 85:3198-3205.
Giese SB, Ahrens P (2000) Detection of Mycobacterium avium subsp. paratuberculosis in milk from clinically affected cows by PCR and culture. Vet. Microbiol 77:291-297.
Grant IR, Rowe MT, Dundee L, Hitchings E (2001) Mycobacterium avium ssp. paratuberculosis: its incidence, heat resistance and detection in milk and dairy products. Int J Dairy Technol 54:2-13.
Gwozdz JM, Thompson KG, Murray A, West DM, Manktelow BW (2000) Use of the polymerase chain reaction assay for the detection of Mycobacterium avium subspecies paratuberculosis in blood and liver biopsies from experimentally infected sheep. Aust Vet J 78:622-624.
Hasonova L, Trcka I, Babak V, Rozsypalova Z, Pribylova R, Pavlik I (2009) Distribution of Mycobacterium avium subsp. Paratuberculosis in tissues of naturally infected cattle as affected by age. Vet Med 54(6):257-269.
Ikonomopoulos J, Pavlik I, Bartos M, Svastova P, Ayele WY, Roubal, P, Lukas J, Cook N, Gazouli M (2005) Detection of Mycobacterium avium subsp. Paratuberculosis in retail cheeses from Greece and the Czech Republic.Appl Environ Microbiol 71:8934-8936.
McDonald WL, O’Riley KJ, Schroen CJ, Condrom RJ (2005) Heat inactivation of Mycobacterium avium subsp. paratuberculosis in milk. Appl Environ Microbiol 71:1785-1789.
Miller JM, Jenny AL, Ellingson JL (1999) Polymerase chain reaction identification of Mycobacterium avium in formalinfixed, paraffin-embedded animal tissues. J Vet Diagn Invest 11:436-440.
Mutharia LM, Klassen MD, Fairles J, Barbut S, Gill CO (2010)Mycobacterium avium subsp. paratuberculosis in muscle, lymphatic and organ tissues from cows with advanced Johne’s disease. Int J Food Microbiol 136:340-344.
Nolte FS, Metchock B, McGowan Jr JE, Edwards A, Okwumabua O, Thurmond C, Mitchell PS, Plikaytis B, Shinnick T (1993). Direct Detection of Mycobacteriumtuberculosis in Sputum by Polymerase Chain Reaction and DNA Hybridization. J Clin Microbiol 31(7):1777-1782.
Pribylova R, Slana I, Kralik P, Kralova A, Babak V, Pavlik I (2011) Correlation of Mycobacterium avium subsp. Paratuberculosis counts in gastrointestinal tract, muscles of the diaphragm and the masseter of dairy cattle and potential risk for consumers. Int J Food Microbiol 151:314-318.
Reddacliff LA, Marsh IB, Fell SA, Austin SL, Whittington RJ (2010) Isolation of Mycobacterium avium subspecies paratuberculosis from muscle and peripheral lymph nodes using acid-pepsin digest prior to BACTEC culture.Vet. Microbiol145:122-128.
Reddacliff LA, Vadali A, Whittington RJ (2003) The effect of decontamination protocols on the numbers of sheep strain Mycobacterium avium subsp. paratuberculosis isolated from tissues and faeces. Vet. Microbiol95:271-282.
Stabel JR, Wells SJ, Wagner BA (2002) Relationships between fecal culture, ELISA, and bulk tank milk test results for Johne’s disease in US dairy herds. J Dairy Sci 85:525-531.
Uzoigwe JC, Khaitsa ML, Gibbs PS (2007) Epidemiological evidence for Mycobacterium avium subspecies paratuberculosis as a cause of Crohn’s disease. Epidemiol Infect 135(7):1057-1068.
Whitlock RH, Wells SJ, Sweeney RW, van Tiem J (2000) ELISA and fecal culture for paratuberculosis (Johne’s disease): Sensitivity and specificity of each method. Vet. Microbiol 77:387-398.
Whittington RJ (2009) Factors affecting isolation and identification of Mycobacterium avium subsp. paratuberculosis from fecal and tissue samples in a liquid culture system. J. Clin. Microbiol 47:614-622.