Neosporosis: a neglected abortifacient disease in Egypt, seroprevalence and farmers’ knowledge, attitudes and practices


Keywords:
Neospora caninum cattle Egypt knowledge attitudes and practices abortion
A GABER
YM HEGAZY
AF OREIBY
T ABDEL WAHAB
MH AL-GAABARY
Abstract

Neosporosis has emerged as a serious disease of cattle and dogs worldwide, causing abortions, neonatal mortalities and massive economic losses in dairy herds. The current study aimed to investigate the burden of Neospora (N.) caninum infection among cows that aborted and to assess knowledge, attitudes, and practices (KAPs) of farmers toward neosporosis in Kafrelsheikh governorate, Egypt. A total of 92 cows that aborted and 25 heifers born from cows that aborted and seropositive for N. caninum infection from 15 dairy herds in different districts of Kafrelsheikh governorate were examined serologically against N. caninum infection using ELISA. A structured questionnaire was built and distributed to 41 farmers in the study area. The overall seroprevalence of N. caninum infection among the examined cows that aborted was 38.04% (35/92). On the other hand, the prevalence of N. caninum infection among the 25 examined heifers born from seropositive cows that aborted was 28% (7/25). The KAPs analysis showed that farmers lack the required information on N. caninum infection and its consequences on dairy farms. The farmers performed risky practices which are responsible for disease entrance and spread on the farm; buy and keeping animals after abortion as well as their heifers. In addition, risk management practices were widespread in dairy farms such as: free movement of dogs, especially stray ones, in 100% of the farms, dogs were able to access and defecate in cattle feed and drinking water sources and had the chance to eat placentas and abortion materials. The current study points out neosporosis as a neglected cause of abortion among cattle in Kafrelsheikh governorate, as well as there is a lack of knowledge and risky practices by the local farmers. These findings may be extrapolated to nearby areas and countries of the same cattle husbandry practices, and veterinary services in such countries should consider neosporosis in their surveillance and control programs.

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References
Aguado-Martínez A, Basto AP, Tanaka S, RyserLT, NunesTP, Ortega-Mora LM, Arranz-Solís D, LeitãoA, HemphillA (2019) Immunization with a cocktail of antigens fused with OprI reduces Neospora caninum vertical transmission and postnatal mortality in mice. Vaccine 37: 473-483.
Ahmed NE, Al-Akabway LM , Ramadan MY, Abd El-Gawad SM, Moustafa MMA (2017) Serological and PCR-sequencing assays for diagnosis of Toxoplasma gondii and Neospora caninum infecting camels in Egypt. BVMJ 33: 200-210.
Andreotti R, Barros CJ, Pereira AR, Oshiro LM, Cunha RC, Neto LFF (2010) Association between seropositivity for Neospora caninum and reproductive performance of beef heifers in the Pantanal of Mato Grosso do Sul, Brazil. Rev Bras Parasitol Vet 19: 119-123.
Bărburaş D, Györke A, Blaga R, Bărburaş R, Kalmár Z, Vişan S, Mircean V, Blaizot A, Cozma V (2019) Toxoplasma gondii in water buffaloes (Bubalus bubalis) from Romania: what is the importance for public health? Parasitol Res. 118:2695-703.
Bartels CJ, Wouda W, Schukken YH (1999) Risk factors for Neospora caninum-associated abortion storms in dairy herds in The Netherlands (1995 to 1997). Theriogenology 52: 247-257.
Bartels CJ, Van Schaik G, Veldhuisen JP, Van den Borne BH, Wouda W, Dijkstra T (2006) Effect of Neospora caninum-serostatus on culling, reproductive performance and milk production in Dutch dairy herds with and without a history of Neospora caninum associated abortion epidemics. Prev Vet Med 77:186-198.
Bartley PM, Katzer F, Rocchi MS, Maley SW, Benavides J, Nath M, Pang Y, Cantón G, Thomson J, Chianini F, Innes EA (2013) Development of maternal and foetal immune responses in cattle following experimental challenge with Neospora caninum at day 210 of gestation. Vet. Res 44: 91.
Bjerkas I, Mohn SF, Prestus J. (1984) Unidentified cyst-forming sporozoon causing encephalomyelitis and myositis in dogs. Z Parasitenkd 70: 271-274.
Collantes-Fernández E, Gómez-Bautista M, Miró G, Alvarez-García G, Pereira-Bueno J, Frisuelos C, Ortega-Mora LM (2008) Seroprevalence and risk factors associated with Neospora caninum infection in different dog populations in Spain. Vet Parasitol 152: 148-151.
Darío Cedeño Q, Bibiana Benavides B (2013) Seroprevalence and risk factors associated to Neospora caninum in dairy cattle herds in the municipality of Pasto, Colombia. Rev MVZ Córdoba 18: 3311-3316.
De Aquino Diniz LV, Minutti AF, de Souza Lima Nino B, Costa LR, Bosculo MRM, de Almeida BFM, Garcia JL, de Barros LD (2019) Vertical transmission of Neospora caninum in bovine fetuses from a slaughterhouse in Brazil. Trop Anim Health Prod 51:175-1755.
De Souza SL, Guimares JS, Ferreira F, Dubey JP, Gennari SM (2002) Prevalence of Neospora caninum antibodies in dogs from dairy cattle farms in Parana, Brazil. J Parasitol 88: 408-409.
Dijkstra T, Barkema HW, Eysker M, Wouda W (2001a) Evidence of post-natal transmission of Neospora caninum in Dutch dairy herds. Int J Parasitol 31: 209-215.
Dijkstra T, Eysker M, Schares G, Conraths FJ, Wouda W, Barkema HW (2001b) Dogs shed Neospora caninum oocysts after ingestion of naturally infected bovine placenta but not after ingestion of colostrums spiked with Neospora caninum tachyzoites. Int J Parasitol 31: 747-752.
Dubey JP, Romand S, Hilali M, Kwok OC, Thulliez P (1998) Seroprevalence of antibodies to Neospora caninum and Toxoplasma gondii in water buffaloes (Bubalus bubalis) from Egypt. Int J Parasitol 28:527-529.
Dubey JP, Schares G, Ortega-Mora LM (2007) Epidemiology and control of neosporosis and Neospora caninum. Clin Microbiol Rev 20: 323-367.
Dubey JP, Schares G (2011) Neosporosis in animals - The last five years. Vet Parasitol 180: 90-108.
El-Diasty MM, Ahmed HA, Sayour AE, El Hofy FI, Tahoun AB, Shafik SM (2016) Seroprevalence of Brucella spp. in Cattle, Molecular Characterization in Milk, and the Analysis of Associated Risk Factors with Seroprevalence in Humans, Egypt. Vector Borne Zoonotic Dis 16:758-764.
Fereig RM, AbouLaila MR, Mohamed SG, Mahmoud HYAH, Ali AO, Ali AF, Hilali M, Zaid A, Mohamed AEA, Nishikawa Y (2016) Serological detection and epidemiology of Neospora caninum and Cryptosporidium parvum antibodies in cattle in southern Egypt. Acta Trop 162:
-211.
French NP, Davison HC, Clancy D, Trees AJ (1999) Mathematical models of Neospora caninum infection in dairy cattle, transmission and options of control. Int J Parasitol 29: 691-1704.
Ghalmi F, China B, Ghalmi A, Hammitouche D, Losson B (2012) Study of the risk factors associated with Neospora caninum seroprevalence in Algerian cattle populations. Res Vet Sci 93: 655-661.
Gondim LF, Gao L, McAllister MM (2002) Improved production of Neospora caninum oocysts, cyclical oral transmission between dogs and cattle, and in vitro isolation from oocysts. J Parasitol 88: 1159-63.
Haddad JP, Dohoo IR, VanLeewen JA (2005). A review of Neospora caninum in dairy and beef cattle--a Canadian perspective. Can Vet J 46:230-243.
Hall CA, Reichel MP, Ellis JT (2005) Neospora abortions in dairy cattle: diagnosis, mode of transmission and control. Vet Parasitol 128: 231-241.
Hamidinejat H, Seifi Abad Shapouri MR, Namavari MM, Shayan P, Kefayat M (2015) Development of an Indirect ELISA using different fragments of recombinant Ncgra7 for detection of Neospora caninum infection in cattle and water buffalo. Iran J Parasitol 10: 69-77.
Hilali M, Romand S, Thulliez P, Kwok OC, Dubey JP (1998) Prevalence of Neospora caninum and Toxoplasma gondii antibodies in sera from camels from Egypt. Vet. Parasitol 75:269-271.
Ibrahim HM, Huang P, Salem TA, Talaat RM, Nasr MI, Xuan X, Nishikawa Y (2009) Short report: prevalence of Neospora caninum and Toxoplasma gondii antibodies in northern Egypt.Am J Trop Med Hyg80:263 - 267.
Ibrahim HM (2013) Seroprevalence of Neospora caninum antibodies in chicken samples from Delta Egypt using a recombinant NcSAG1 protein-based ELISA. Egypt J Immunol20:29-37.
Innes EA, Andrianarivo AG, Björkman C, Williams DJ, Conrad PA (2002) Immune responses to Neospora caninum and prospects for vaccination. Trends Parasitol 18: 497-504.
Japa O, Nuangmek A, Prakhammin K, Flynn RJ (2019) Prevalence of vertically transmitted Neospora caninum amongst beef cattle in Phayao. Thailand. Parasitol Int 70: 98-101.
Lagomarsino H, Scioli A, Rodríguez A, Armendano J, Fiorani F, Bence A, García J, Hecker Y, Gual I, Cantón G, Odeón A, Campero C, Moore D (2019) Controlling Endemic Neospora caninum-Related Abortions in a Dairy Herd From Argentina. Front Vet Sci 6: 446.
Llano HA, Guimarães MS, Soares RM, GinaPoloA, Caetanoda S (2018) Seroprevalence and risk factors for Neospora caninum infection in cattle from the eastern Antioquia, Colombia. Vet Anim Sci 6: 69-74.
Lindsay DS, Dubey JP, Duncan RB (1999) Confirmation that the dog is a definitive host for Neospora caninum. Vet Parasitol 82: 327-333.
Moraveji M, Hosseini A, Moghaddar N, Namavari MM, Eskandari MH (2012) Development of latex agglutination test with recombinant NcSAG1 for the rapid detection of antibodies to Neospora caninum in cattle. Vet Parasitol 189:211-217.
Moore DP, Konrad JL, San Martino S, Reichel MP, Cano DB, Méndez S, Späth EJ, Odeón AC, Crudeli G, Campero CM (2014) Neospora caninum serostatus is affected by age and species variables in cohabiting water buffaloes and beef cattle. Vet Parasitol 203:259-263.
Olmo L, Reichel MP, Nampanya S, Khounsy S, Wahl LC, Clark BA, Thomson PC, Windsor PA, Bush RD (2019) Risk factors for Neospora caninum, bovine viral diarrhoea virus and Leptospira interrogans serovar Hardjo infection in smallholder cattle and buffalo in Lao PDR. PloS One 14: 1-25.
Ortega-Mora LM, Gottstein MB, Conraths FJ, Buxton D (2007) Protozoal abortion in farm ruminants: Guidelines for Diagnosis and Control. 1st ed, Athenaeum Press, Oxfordshire, UK: pp 302.
Perry B, Grace D (2009) The impacts of livestock diseases and their control on growth and development processes that are pro-poor. Philos Trans R Soc Lond B Biol Sci 364:2643-2655.
Reichel MP, Alejandra Ayanegui-Alcérreca M, Gondim LF, Ellis JT (2013) What is the global economic impact of Neospora caninum in cattle - the billion dollar question. Int J Parasitol 43: 133-142.
Ribeiro CM, Soares IR, Mendes RG, de Santis Bastos PA, Katagiri S, Zavilenski RB, de Abreu HFP, Afreixo V (2019) Meta-analysis of the prevalence and risk factors associated with bovine neosporosis. Trop Anim Health Prod 51: 1783-1800.
Santolaria P, Almeria S, Martinez-Bello D, Nogareda C, Mezo M, Gonzalez-Warleta M, Castro-Hermida JA, Pabón M, Yániz JL, López-Gatius F (2011) Different humoral mechanisms against Neospora caninum infection in pure breed and crossbreed beef/dairy cattle pregnancies. Vet. Parasitol 178: 70-76.
Semango G, Hamilton CM, Kreppel K, Katzer F, Kibona T, Lankester F, Allan KJ, Thomas KM, Claxton JR, Innes EA, Swai ES, Buza J, Cleaveland S, de Glanville WA(2019) The Sero-epidemiology of Neospora caninum in Cattle in Northern Tanzania. Front Vet Sci6:327.
Shalaby Y, Mousa W, Elbaz HT, Sherif HR, Nayel M, Elsify A, Zaghawa A, Salama A, Elmenshaway A, Hadad GA, Shaker A, Eldosoky I, Heavin H (2019) Serological and Molecular Diagnosis of Bovine Brucellosis in Menoufia Province. JCVR 1:115-126.
Söndgen P, Peters M, Bärwald A, Wurm R, Holling F, Conraths FJ, Schares G (2001) Bovine neosporosis: immunoblot improves foetal serology. Vet. Parasitol 102: 279-290.
Trees AJ, Williams DJ (2005) Endogenous and exogenous transplacental infection in Neospora caninum and Toxoplasma gondii. Trends Parasitol 21:558-561.
Vasbinder MA, Locke P (2016) Introduction: global laws, regulations, and standards for animals in research. ILAR journal 57(3): 261-265.
Vianna LC, Sartor IF, Pituco EM, Okuda LH, Camargo CN, Kronka SN (2008) Incidence and transplacental transmission of Neospora caninum in primiparous females from Bosindicus slaughtered in Presidente Prudente, SãoPaulo, Brazil. Semina. Ciências Agrárias, Londrina29:
-392.
Weston JF, Heuer C, Williamson NB (2012) Efficacy of a Neospora caninum killed tachyzoite vaccine in preventing abortion and vertical transmission in dairy cattle. Prev Vet Med 103: 136-144.
Williams DJ, Hartley CS, Björkman C, Trees AJ (2009) Endogenous and exogenous transplacental trasmission of Neospora caninum - how the route of transmission impacts on epidemiology and control of disease. Parasitology 136: 1895-1900.
Wilson DJ, Orsel K, Waddington J, Rajeev M, Sweeny AR, Joseph T, Grigg ME, Raverty SA (2016) Neospora caninum is the leading cause of bovine fetal loss in British Columbia, Canada. Vet Parasitol 218:46-51.