Impact of stocking density and dietary nano-zinc supplementation on stress indicators, immunity, and DNA damage in broiler chickens

PDF (English)
Опубликован: Απρ 18, 2023
Обновлен: 2023-04-18
Версии:
2023-04-18 (2)
DOI: https://doi.org/10.12681/jhvms.28713
Ö Sevim
Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary Medicine, Aydın Adnan Menderes University, Işıklı, Efeler, Aydın 09016, Turkey.
https://orcid.org/0000-0001-6123-4159
M Boyacıoğlu
ÖB Yılmaz
AK Aydın
ÖS Özdemir
A Reman Temiz
EK Khamseh
U Ahsan
E Kuter
O Tatli
EK Yıldırım
Аннотация
Effects of stocking density (SD) and nano zinc on oxidative status, immune function, and DNA damage in broilers were examined in the current study. Totally 480 one-day-old male broilers (Ross 308) were randomly divided into 4 treatments each contained 8 replicates.  A 2 ´ 2 factorial arrangement with two groups for dietary zinc (Zn) form (inorganic or nano) and two groups for SD (low = 12 birds/m2 and high = 18 birds/m2) was conducted. Basal diets based on the corn-soybean meal were formulated. Relative liver weight was lower (P<0.05) in broiler subjected high stocking density (HSD) but other lymphoid organ weights did not affect. Relative lymphoid organ weights were not affected by dietary nano zinc. HSD increased the serum corticosterone level of broiler (P<0.05) but did not affect the blood heterophil:lymphocyte ratio, serum glutathione, superoxide dismutase, and malondialdehyde levels. Dietary nano zinc had no effect on these stress indicators. Serum interferon-gamma, interleukin 12, and interleukin 18 levels were not affected by HSD. Dietary nano zinc tended to reduce the interferon-gamma level (P=0.05) but did not affect interleukin 12, and interleukin 18 levels in broiler. Eeither HSD or nano zinc had no effect on DNA damage in lymphocyte. In conclusion, HSD may decrease the relative lymphoid organ weights and increase the stress indicators but did not affect the immunity and DNA damage. Generally, dietary nano zinc had no effect on stress indicators, immunity, and DNA damage in broiler.
Article Details
  • Раздел
  • Research Articles
Лицензия Creative Commons

Это произведение доступно по лицензии Creative Commons «Attribution-NonCommercial» («Атрибуция — Некоммерческое использование») 4.0 Всемирная.

Authors who publish with this journal agree to the following terms:

· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Скачивания
Данные скачивания пока недоступны.
Биография автора
Ö Sevim, Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary Medicine, Aydın Adnan Menderes University, Işıklı, Efeler, Aydın 09016, Turkey.

Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary Medicine - DVM PhD

Библиографические ссылки
Anwar MI, Awais MM, Akhtar M, Navid MT, Muhammad F (2019) Nutritonal and immunological effects of nano-particles in commercial poultry birds. World Poult Sci J 75: 261-273.
AOAC (2000) Official Methods of Analysis. 17th ed, Association of Official Analytical Chemists International, Virginia, USA.
Aviagen (2014) Ross 308 Broiler: Nutrition Specifications. Aviagen, Scotland, UK.
Attia H, Nounou H, Shalaby M (2018) Zinc oxide nanoparticles induced oxidative DNA damage, inflammation and apptosis in rat’s brain after oral exposure. Toxics 6 (2): 29-49.
Asheer M, Manwar SJ, Gole MA, Sırsat S, Wade MR, Khose KK, Sajid Ali S (2018) Effect of dietary nano zinc oxide supplementation on performance and zinc bioavailability in broilers. Indian J Poult Sci 53 (1): 70-75.
Beutler E, Duron O, Kelly BM (1963) Improved method for the determination of blood glutathion. J Lab Clin Med 61: 882-888.
Boyacioglu M, Sekkin S, Kum C, Korkmaz D, Kiral F, Yalinkilinc HS, Ak MO, Akar F (2014) The protective effects of vitamin C on the DNA damage, antioxidant defenses and aorta histopathology in chronic hyperhomocysteinemia induced rats. Exp Toxicol Pathol 66: 407-413.
Cai CH, Zhao RX, Wang P, Wang JS, Li KX, Zhan XA, Wang KY (2019) Effects of different stocking densities on growth performance, antioxidant ability, and immunity of finishing broilers. Anim Sci J 90: 583-588.
Campbell TW (1988) Avian Hematology and Cytology. Iowa State University Press, Ames, IA.
Cengiz Ö, Köksal BH, Tatlı O, Sevim Ö, Ahsan U, Üner AG, Ulutaş PA, Beyaz D, Büyükyörük S, Yakan A, Önol AG (2015) Effect of dietary probiotic and high stocking density on the performance, carcass yield, gut microflora, and stress indicators of broilers. Poult Sci 94: 2395-2403.
Collins AR, Dobson VL, Dusinska M, Kennedy G, Stetina R (1997) The comet assay: what can it really tell us?. Mutat Res-Fund Mol M 375: 183-193.
Dawkins MS, Donnely CA, Jones TA (2004) Chicken welfare is influenced more by housing conditions than by stocking density. Nature 427: 342-344.
Dozier WA, Thaxton JP, Purswell JL, Olanrewaju HA, Branton SL, Roush WB (2006) Stocking density effects on male broilers grown to 1.8 kilograms of body weight. Poult Sci 85: 344–351.
Dukare Sagar P, Mandal AB, Akbar N, Dinani OP (2018) Effect of different levels and sources of zinc on growth performance and immunity of broiler chicken during summer. Int J Curr Microbiol Appl Sci 7 (5): 459-471.
Fairbanks VF, Klee GG (1999) Biochemical aspects of heamatology. In: Burtis CA, Ashwood ER (eds), Tietz Textbook of Clinial Chemistry. 3rd ed WB Saunders Company, Philadelphia.
Hafez A, Nasse E, Fahmy M, Elsabagh M, Bakr A, Hegazi E (2020) Impact of dietary nano-zinc oxide on immune response and antioxidant defense of broiler chickens. Environ Sci Pollut Res 27: 19108-19114.
Houshmand M, Azhar K, Zulkifli I, Bejo MH, Kamyab A (2012) Effects of prebiotic, protein level, and stocking density on performance, immunity, and stress indicators of broilers. Poult Sci 91: 393–401.
Hussan F, Kirishna D (2018) Effect of dietary ınclusion of nano zinc oxide in commercial broilers. Thesis, PVNR Veterinary University, Department of Poultry Science, Hyderabad, India.
Jang IS, Yun SH, Ko YH, Kim SY, Song MH, Kim JS, Sohn SH, Moon YS (2014) The effect of stocking density and strain on the performance and physiological adaptive responses in broiler chickens. Korean J Poult Sci 41: 205-215.
Jastrebski SF, Lamont SJ, Schmidt CJ (2017) Chicken hepatic response to chronic heat stress using integrated transcriptome and metabolome analysis. PloS One 12 (7): e0181900.
Kang SY, Ko YH, Moon YS, Sohn SH, Jang IS (2011) Effects of the combined stress induced by stocking density and feed restriction on hematological and cytokine parameters as stress indicators in laying hens. Asian-Australas J Anim Sci 24 (3): 414-420.
Mohammadi V, Ghazanfari S, Mohammadi-Sangcheshmeh A, Nazaran MH (2015a) Comparative effects of zinc-nano complexes, zinc-sulphate and zincmethionine on performance in broiler chickens. Br Poult Sci 56 (4): 486-493.
Mohammadi F, Ahmadi F, Amiri Andi M (2015b) Effect of zinc oxide nanoparticles on carcass parameters, relative weight of digestive and lymphoid organs of broiler fed wet diet during the starter period. Int J Biosci 6 (2): 389-394.
Quaid M, Albastan H, Shafey T, Hussein E, Abudabos AM (2016) Effect of stocking density on the performance and immunity of 1- to 14-d-old broiler chicks. Rev Bras Cienc Avic 18 (4): 683-692.
Ramiah SK, Awad EA, Mookiah S, Idrus Z (2019) Effects of zinc oxide nanoparticles on growth performance and concentrations of malondialdehyde, zinc in tissues, and corticosterone in broiler chickens under heat stres conditions. Poult Sci 98: 3828-3838.
Rossi P, Rutz F, Anciuti MA, Rech JL, Zauk NHF (2007) Influence of graded levels of organic zinc on growth performance and carcass traits of broilers. J Appl Poult Res 16: 219-225.
Simitzis PE, Kalogeraki E, Golimytis M, Charismiadou MA, Triantaphyllopoulos K, Ayoutanti A, Niforou K, Hager-Thedorides AL, Deligeorgis SG (2012) Impact of stocking density on broiler growth performance, meat characteristics, behavioural components and indicators of physiological and oxidative stress. Br Poult Sci 53 (6): 721-730.
Sloup V, Jankovska I, Nechybova S, Perinkova P, Langrova I (2017) Zinc in the animal organism: a review. Sci Agric Bohem 48 (1): 13-21.
Sohn SH, Subramani VK, Moon YS, Jang IS (2012) Telomeric DNA quantity, DNA damage, and heat shock protein gene expression as physiological stress markers in chickens. Poult Sci 91: 829-836.
Song Y, Leonard SW, Traber MG, Ho E (2009) Zinc deficiency affects DNA damage, oxidative stress, antioxidant defences, and DNA repair in rats. J Nutr 139 (9): 1626-1631.
Sun Y, Oberley LW, Li Y (1988) A simple for clinical assay of superoxide dismutase. Clin Chem 34: 497-500.
Uzum MH, Oral Toplu HD (2013) Effects of stocking density and feed restriction on performance, carcass, meat quality characteristics and some stress parameters in broilers under heat stress. Rev Med Vet 164 (12): 546-554.
Van Soest PJ, Robertson JB, Lewis BA (1991) Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci 74 (10): 3583- 3597.
Yoshioka T, Kawada K, Shimada T, Mori M (1979) Lipid peroxidation in maternal and cord blood and protective mechanism against activated-oxygen toxicity in the blood. Am J Obstet Gynecol 135: 372-376.
Zhao CY, Tan SX, Xiao XY, Qiu XS, Pan JQ, Tang ZX (2014) Effects of dietary zinc oxide nanoparticles on growth performance and antioxidative status in broilers. Biol Trace Elem Res 160: 361-367.