In vitro evaluation of a natural food supplement as inhibitors of feline herpesvirus replication
Аннотация
The feline herpesvirus (FHV) is a widely diffused and highly contagious virus that represents a common health problem in cats. It is frequently associated with diseases of different pathogenicity that can be particularly severe in young kittens determining viral pneumonia and sometimes, death. Unfortunately, there isn’t a well-defined therapeutic protocol against the virus. Several studies concerning the application of alternative treatments against herpesviruses have been performed with promising results, in both human and veterinary medicine. The present study aims to investigate in vitro antiviral effects against FHV of the commercially compound HELP-TH1 (Camon, S.p.A., Italy) mainly composed by Ganoderma lucidum, Cordyceps sinensis, and Trametes versicolor. The antiviral effect of HELP-TH1 was evaluated by the Plaque Reduction Assay and by the real time PCR quantifying and comparing the viral load of infected controls vs treated experimental samples. Our data indicate that HELP-TH1 inhibits the cytopathic effect of the virus and reduces the viral load in the experimental conditions demonstrating its antiviral effect.
Article Details
- Как цитировать
-
Pacini, M., Bonaccini, P., Mazzei, M., & Forzan, M. (2024). In vitro evaluation of a natural food supplement as inhibitors of feline herpesvirus replication. Journal of the Hellenic Veterinary Medical Society, 74(4), 6377–6384. https://doi.org/10.12681/jhvms.30136
- Выпуск
- Том 74 № 4 (2023)
- Раздел
- Research Articles
Это произведение доступно по лицензии Creative Commons «Attribution-NonCommercial» («Атрибуция — Некоммерческое использование») 4.0 Всемирная.
Authors who publish with this journal agree to the following terms:
· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
Скачивания
Данные скачивания пока недоступны.
Библиографические ссылки
Akbar R, Yam WK (2011) Interaction of ganoderic acid on HIV related
target: molecular docking studies. Bioinformation 7: 413-417.
Astani A, Reichling J, Schnitzler P (2011) Screening for antiviral activities of isolated compounds from essential oils. Evid Based Complement Alternat Med 253643.
Binns SH, Dawson S, Speakman AJ, Cuevas LE, Hart CA, Gaskell CJ,
Morgan KL, Gaskell RM (2000). A study of feline upper respiratory
tract disease with reference to prevalence and risk factors for infection with feline calicivirus and feline herpesvirus. J Feline Med Surg
:123-33.
Blanco K, Prendas J, Cortes R, Jimenez C, Dolz G (2009) Seroprevalence of viral infections in domestic cats in Costa Rica. J Vet Med
Sci71:661-3.
Chattopadhyay D,Das S,Chakraborty S, Bhattacharya SK (2010) Ethnomedicines for the development of anti-herpesvirus agents. Ethnomedicine: A Source of Complementary Therapeutics117-147.
Buhner SH (2021). Herbal Antivirals: Natural Remedies for Emerging &
Resistant Viral Infections. Storey Publishing.
Chen J,Zhang W,Lu T,Li J,Zheng Y,Kong L (2006) Morphological and genetic characterization of a cultivated Cordyceps sinensis fungus and
its polysaccharide component possessing antioxidant property in H22
tumor-bearing mice. Life Sciences78: 2742-2748.
Chien CM,Cheng JL, Chang WT,Tien MH,Tsao CM,Chang YH, Chang
HY,Hsieh JF,Wong CH,Chen ST (2004) of Ganoderma lucidum alter cell immunophenotypic expression and enhance CD56 + NK-cell
cytotoxicity in cord blood. Bioorganic and Medicinal Chemistry12:
-5609.
Cruz A, Pimentel L, Rodríguez-Alcalá LM, Fernandes T, Pintado M
(2016) Health Benefits of Edible Mushrooms Focused on Coriolus
versicolor: A Review.Journal of Food and Nutrition Research4: 773-
Elkhateeb WA,Daba GM, Elmahdy EM,Thomas PW, Wen TC,Shaheen
MNF (2019) Antiviral Potential of Mushrooms in the Light of their
Biological Active Compounds. ARC Journal of Pharmaceutical Sciences (AJPS) 5: 45-49.
Eo SK, Kim YS, Lee CKHanSS (1999). Antiherpetic activities of various
protein bound polysaccharides isolated from Ganoderma lucidum. J.
Ethnopharmacol., 68: 175-181
ErtlR, Klein D (2014) Transcriptional profiling of the host cell response to
feline immunodeficiency virus infection. Virol J 11, 52
Ferreira IC, Vaz JA, Vasconcelos MH, Martins A (2010) Compounds from
wild mushrooms with antitumor potential. Anticancer Agents Med
Chem10:424-36.
Forzan M, Pacini MI, Bonaccini P, Mazzei M. (2022). Antiviral effect of a
commercially phytotherapeutic compound against feline immunodeficiency virus. NatProd Res 16: 4159-4164.
Garber A, Barnard L, Pickrell C (2021) Review of whole plant extractswithactivity against herpes simplex viruses in vitro and in vivo.
J Evid Based Integr Med 26:1-57.
Gaskell R, Dawson S, Radford A, Thiry E (2007) Feline herpesvirus. Vet
Res38:337-54.
Gaskell R, Dennis PE, Goddard LE, Cocker FM, Wills JM (1985) Isolation of felid herpesvirus 1 from the trigeminal ganglia of latently
infected cats. J Gen Virol 66: 391-94
Gaskell RM, Dawson S, Radford A. Feline Respiratory Disease (2012)
In: Green CE,Infectious diseases of the dog and cat. 4th edition. St
Louis, MO pp 151-162
Gaskell RM, Povey RC (1977) Experimental induction of feline viral
rhinotracheitis virus re-excretion in FVR-recovered cats. Vet Rec
:128-33.
Gould D (2011) Feline Herpesvirus-1: Ocular Manifestations, Diagnosis and Treatment Options. Journal of Feline Medicine and Surgery13:333-346.
Habtemariam S (2020) Trametes versicolor (Synn. Coriolus versicolor)
Polysaccharides in Cancer Therapy: Targets and Efficacy. Biomedicines 8, 135.
Helps CR, Lait P, Damhuis A, Björnehammar U, Bolta D, Brovida C,
Chabanne L, Egberink H, Ferrand G, Fontbonne A, Pennisi MG,
Gruffydd-Jones T, Gunn-Moore D, Hartmann K, Lutz H, Malandain
E, Möstl K, Stengel C, Harbour DA, Graat EA (2005) Factors associated with upper respiratory tract disease caused by feline herpesvirus,
feline calicivirus, Chlamydophila felis and Bordetella bronchiseptica
in cats: experience from 218 European catteries. Vet Rec156:669-73.
Ho CY, Lau CB, Kim CF, Leung KN, Fung KP, Tse TF, Chan HH, Chow
MS (2004) Differential effect of Coriolus versicolor (Yunzhi) extract
on cytokine production by murine lymphocytes in vitro. International
Immunopharmacology 4:1549-1557
Holliday JC,Cleaver M (2008) Medicinal value of the caterpillar fungi
species of the genus Cordyceps (Fr.) link (Ascomycetes). A review
(2008). International Journal of Medicinal Mushrooms10: 219-234.
Jedrzejewski T, Piotrowski J, Kowalczewska M, Wrotek S, Kozak W
(2015).Polysaccharide peptide from Coriolus versicolor induces interleukin 6-related extension of endotoxin fever in rats. International
Journal of Hyperthermia 31:626-634.
Jędrzejewski T, Piotrowski J, Pawlikowska M, Wrotek S, Kozak W
(2019). Extract from Coriolus versicolor fungus partially prevents
endotoxin tolerance development by maintaining febrile response and
increasing IL-6 generation. Journal of Thermal Biology 83:69-79.
Kim YS, Eo SK, Oh KW, Lee CK, Han SS (2000) Antiherpetic activities
of acidic protein bound polysaccharide isolated from Ganoderma lucidum alone and in combinations with interferons. J. Ethnopharmacol
: 451-458.
Krupodorova T, Rybalko S, Barshteyn V (2014) Antiviral activity of Basidiomycete mycelia against influenza type A (serotype H1N1) and
herpes simplex virus type 2 in cell culture. Virol. Sin29: 284-290.
Kuo YC, Tsai WJ,Shiao MS,Chen CF, Lin CY (1996) Cordyceps sinensis
as an immunomodulatory agent, American Journal of Chinese Medicine24: 111-125.
Lee CL, Yang X, Wan JMF (2006) The culture duration affects the immunomodulatory and anticancer effect of polysaccharopeptide derived
from Coriolus versicolor. Enzyme and Microbial Technology 38:14-
Lee Y, Maes R, Tai SS, Soboll Hussey G (2019). Viral replication and
innate immunity of feline herpesvirus-1 virulence-associated genes in
feline respiratory epithelial cells. Virus Res264:56-67.
Li F, Wen H, Zhang Y, Aa M, Liu X (2011) Purification and characterization of a novel immunomodulatory protein from the medicinal mushroom Trametes versicolor. Sci China Life Sci54:379-85.
Lickey AL, Kennedy M, Patton S, Ramsay EC (2005) Serologic survey of
domestic felids in the Petén region of Guatemala. J Zoo Wildl Med
: 121-123.
Liu J, Yang F, Ye LB, Yang XJ, Timani KA, Zheng Y, Wang YH (2004)
Possible mode of action of antiherpetic activities of a proteoglycan
isolated from the mycelia of Ganoderma lucidum in vitro. J Ethnopharmacol95:265-72.
Low HC, Powell CC, Veir JK, Hawley JR,LappinMR(2007) Prevalence of
feline herpesvirus 1,Chlamydophila felis, andMycoplasmaspp DNA
in conjunctival cells collected from cats with and without conjunctivitis. American Journal of Veterinary Research 68: 643-648.
Manoj R,Earanna N,Chandranaik BM (2017) Molecular Characterization
of Trametes Species and Screening their Aqueous Extracts for Antiviral Properties. Trends in Biosciences 10.
Mazzei M, Vascellari M, Zanardello C, Melchiotti E, Vannini S, Forzan
M, Marchetti V, Albanese F, Abramo F (2019) Quantitative real time
polymerase chain reaction (qRT-PCR) and RNAscope in situ hybridization (RNA-ISH) as effective tools to diagnose feline herpesvirus-1-associated dermatitis. Vet Dermatol30:491-e147.
Mochizuki M, Kawakami K, Hashimoto M, Ishida T (2000) Recent epidemiological status of feline upper respiratory infections in Japan. J
Vet Med Sci 62: 801-803.
Murayama T, Yamaguchi N, Iwamoto K, Eizuru Y(2006). Inhibition of
ganciclovir-resistant human cytomegalovirus replication by Kampo (Japanese herbal medicine). Antiviral Chemistry and Chemotherapy,
(1), 11-16.
Nguyen D, Barrs VR, Kelman M, Ward MP (2019) Feline upper respiratory tract infection and disease in Australia. Journal of Feline Medicine
and Surgery21:973-978.
Oh KW, Lee CK, Kim YS, Eo SK, Han SS (2000) Antiherpetic activities of acidic protein bound polysacchride isolated from Ganoderma
lucidum alone and in combinations with acyclovir and vidarabine. J
Ethnopharmacol 72: 221-227
Özgör E, Ulusoy M, Çelebier İ, Yildiz SS, Keskin N (2016)Investigation
of Antimicrobial Activity of Different Trametes versicolor Extracts
on Some Clinical Isolates. Hacettepe J Bioland Chem43: 267-272
Pfaffl MW, Horgan GW, DempfleL (2002) Relative expression software
tool (REST) for group-wise comparison and statistical analysis of
relative expression results in real-time PCR. Nucleic Acids Res 30.
Saleh MH, Rashedi I,Keating A (2017) Immunomodulatory properties of
coriolus versicolor: The role of polysaccharopeptide, Frontiers in Immunology8: 1087.
Sanodiya BS, Thakur GS, Baghel RK, Prasad GB, Bisen PS (2009) Ganoderma lucidum: a potent pharmacological macrofungus. Curr Pharm
Biotechnol. 10:717-42.
Shahzad F, Anderson D, Najafzadeh M (2020) The Antiviral, Anti-Inflammatory Effects of Natural Medicinal Herbs and Mushrooms and
SARS-CoV-2 Infection. Nutrients12: 2573
Standish LJ, Wenner CA, Sweet ES, Bridge C, Nelson A, Martzen M, Novack J, Torkelson C (2008)Trametes versicolor mushroom immune
therapy in breast cancer. J Soc Integr Oncol6:122-128.
Summers SC, Ruch-Gallie R, Hawley JR, Lappin MR (2017). Effect of
modified live or inactivated feline herpesvirus-1 parenteral vaccines
on clinical and laboratory findings following viral challenge. Journal
of feline medicine and surgery, 19(8), 824-830.
Tahmasbi S F, Revell M A, Tahmasebi N (2021). Herbal medication to
enhance or modulate viral infections. Nursing Clinics 56(1): 79-89.
Teplyakova T and Kosogova T (2015). Fungal Bioactive Compounds with
Antiviral Effect. Journal of Pharmacy and Pharmacology 3: 357-371.
Thiry E, Addie D, Belák S, Boucraut-Baralon C, Egberink H, Frymus T,
... Horzinek M C (2009). Feline herpesvirus infection. ABCD guidelines on prevention and management. Journal of Feline Medicine &
Surgery, 11(7), 547-555.
Thomasy SM, Maggs DJ. A review of antiviral drugs and other compounds with activity against feline herpesvirus type 1. Vet Ophthalmol19:119-30.
Tian P (2011) Efficacy of Cordyceps sinensis in long term treatment of
renal transplant patients. Frontiers in Bioscience 3: 301-7.
van de Sand L, Bormann M, Schmitz Y, Heilingloh CS, Witzke O, Krawczyk A (2021). Antiviral Active Compounds Derived from Natural
Sources against Herpes Simplex Viruses. Viruses 13: 1386.
Wang J, Liu YM, Cao W, Yao KW, Liu ZQ, Guo JY (2012) Anti-inflammation and antioxidant effect of Cordymin, a peptide purified from
the medicinal mushroom Cordyceps sinensis, in middle cerebral artery occlusion-induced focal cerebral ischemia in rats. Metab Brain
Dis 27:159-65.
Wang PY, Zhu XL, Lin ZB (2012) Antitumor and immunomodulatory effects of polysaccharides from broken-spore of Ganoderma lucidum,
Frontiers in Pharmacology3:135
Yan JK, Wang WQ, Wu YJ (2014) Recent advances in Cordyceps sinensis
polysaccharides: Mycelial fermentation, isolation, structure, and bioactivities: A review, Journal of Functional Foods 6: 33-47.
Yasmin AR, Chia SL, Looi QH, Omar AR, Noordin MM, Ideris A (2020)
Herbal Extracts as Antiviral Agents. In Feed Additives, Aromatic
Plants and Herbs in Animal Nutrition and Health, Academic Press,
London, pp. 115-132.
Yue K, Ye M, Zhou Z, Sun W, Lin X (2013). The genus Cordyceps: a
chemical and pharmacological review. J Pharm Pharmacol 65:474-
Zhao R,Chen Q, He YM (2018) The effect of Ganoderma lucidum extract
on immunological function and identify its anti-tumor immunostimulatory activity based on the biological network, Scientific Reports.
: 12680.
Zhu YL, Hu MQ, Li J, Chen Y, Jia R, Shen S, Zeng Y (2016) In Vitro
Anti-HIV-1 Activity of Cordyceps sinensis Extract. Bing Du Xue
Bao32:417-22.
Zicola A, Saegerman C, Quatpers D, Viandier J, Thiry E (2009) Feline
herpesvirus 1 and feline calicivirus infections in a heterogeneous cat
population of a rescue shelter. J Feline Med Surg 11:1023-1027
