Effects of In-Ovo Glucose and Glutamine Treatment on Hatching Efficiency, Intestinal Histomorphology and Gene Expression of Digestive Enzymes in Broiler Chicks

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Опубликован: Oct 31, 2023
Обновлен: 2023-10-31
Версии:
2023-10-31 (2)
DOI: https://doi.org/10.12681/jhvms.30895
F Cönerr
International Center for Livestock Research and Training, Ankara, Turkey
P Saçaklı
Ankara University, Faculty of Veterinary Medicine, Department of Animal Nutrition and Nutritional Diseases, Ankara, Turkey
Аннотация

The purpose of the present study was to identify the effects of in-ovo injection of glucose and glutamine on hatching efficiency, hatching weight, liver and residual yolk weights, intestinal histomorphology and gene expressions of digestive enzymes. On the 17th day of incubation, NaCl (0.9 %), glutamine (0.5 ml 10%), glucose (0.5 ml 0.25 g/ml), and glutamine+glucose (0.25 ml 10%+0.25 ml 0.25 g/ml) were injected into the amniotic sac. In-ovo injection of glucose, glutamine and glucose+glutamine significantly decreased hatching efficiency in glucose and glutamin+glucose groups (p<0.001). This treatment did not affect hatching weight, liver and residual yolk weight in any of the groups. When the intestinal histomorphology was evaluated, in-ovo injection was found to increase the villi height/crypt depth ratio, villus width and the number of goblet cells in the jejunum; whereas villi height, crypt depth, and tunica muscularis thickness were not significantly affected by the treatments. Moreover, the ileum histomorphology in general appear not to be affected by any of the treatments as well. In-ovo glutamine injection significantly increased Suc-Iso and mTOR gene expressions (p≤0.05) compared to the control group, whereas SGLT1 gene expression was statistically indifferent. At the end of the study, while in-ovo glucose and glutamine injection had an overall negative effect on the hatching efficiency in broiler chicks.

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Библиографические ссылки
Abdulkarimi R, Shahir MH, Daneshyar M (2019) Effects of dietary glu
tamine and arginine supplementation on performance, intestinal mor
phology and ascites mortality in broiler chickens reared under cold
environment. Asian-australas J Anim Sci 32(1): 110-117.
Ar A (1991) Roles of water in eggs. In D. C. Deeming and M. W. J. Fergu
son (eds.). ‘’Egg Incubation: Its Effects on Embryonic Development
in Birds and Reptiles.’’ Cambridge University Press 229-243.
Ar A, Rahn H (1980) Water in the avian egg overall budget of incubation,
Am Zool, 20(2): 373-384.
Bartell SM, Batal AB (2007) The effect of supplemental glutamine on
growth performance, development of the gastrointestinal tract and
humoral immune response of broilers. Poult Sci J 86: 1940-1947.
Basuroy S, Sheth P, Mansbach CM, Rao RK (2005) Acetaldehyde disrupts
tight junctions and adherens junctions in human colonic mucosa: pro
tection by EGF and l-glutamine. Am J Physiol - Gastrointest Liver
Physiol 289: 367-375.
Bhattacharyya A, Majumdar S, Bhanja SK, Mandal AS, Kadam M (2018)
Effect of maternal dietary manipulation and inovo injection of nutri
ents on the hatchability indices, post-hatch growth, feed consump
tion, feed conversion ratio and immunocompetence traits of turkey
poults. J Appl Anim Res 46(1): 287-294.
Cahu CL, Infante JLZ (1995) Effect of the molecular form of dietary ni
trogen supply in sea bass larvae: Response of pancreatic enzymes and
intestinal peptidases. Fish Physiol Biochem 14(3): 209-214.
Calder PC, Yaqoob P (1999) Glutamine and the immune system. Amino
Acids 17: 227-241.
Campos AMA, Rostagno HS, Gomes PC, Silva EA, Albino LFT, Noguei
ra ET (2011) Efeito da inoculação de soluções nutritivas in ovo sobre
a eclodibilidade e o desempenho de frangos de corte. Rev Bras Zoo
tec 40: 1712-1717.
Caspary WF (1992) Physiology and pathophysiology of intestinal absorp
tion. Am J Clin Nutr 55: 299-308.
Chen W, Wang R, Wan HF, Xiong XL, Peng P, Peng J (2009) Influence of
in ovo injection of glutamine and carbohydrates on digestive organs
and pectoralis muscle mass in the duck. Br Poult Sci 50(4): 436-442.
Cheng ZD, Gatlin M, Buentello A (2012) Dietary supplementation of
arginine and/or glutamine influences growth performance, immune
responses and intestinal morphology of hybrid striped bass (Morone
chrysops × Morone saxatilis). Aquac 362-363: 39-43.
Cheng ZY, Buentello A, Gatlin DM (2011) Effects of dietary arginine and
glutamine on growth performance, immune responses and intestinal
structure of red drum, Sciaenops ocellatus. Aquac 319: 247-252.
Colker CM, Swain MA, Fabrucini B, Shi Q, Kaiman DS (2000) Effects of
supplemental protein on body composition and muscular strenght in
healthy atletic male adults. Curr Ther Res 61(1): 19-28.
Coster J, McCauley R, Hall J (2003) Role of specific amino acids in nutri
tional support. ANZ J Surg 73: 846-849.
Dos Santos TT, Corzo A, Kidd MT, McDaniel CD, Torres Filho RA, Araú
jo LF (2010) Influence of in ovo inoculation with various nutrients
and egg size on broiler performance. J Appl Poult Res 19: 1-12.
Floc’h N, S’eve B (2000) Protein and amino acid metabolism in the intes
tine of the pig: from digestion to appearance in the portal vein. Prod
Anim 13: 303-314.
Garcia-de-Lorenzo A, Zarazaga A, Garcia-Luna PP, Gonzalez-Huix F,
Martinez JL, Mijan A, Quecedo L, Casimiro C, Usan L, Del Llano
J (2003) Clinical evidence for enteral nutritional support with gluta
mine: A systematic review. Nutrition 19: 805-811.
Geier MS, Torok VA, Guo P, Allison GE, Boulianne M, Janardhana V,
Bean AG, Hughes RJ (2011) The effects of lactoferrin on the intesti
nal environment of broiler chickens. Br Poult Sci 52: 564-572.
Gholipour V, Chamani M, Shahryar HA, Sadeghi A, Aminafshar M
(2019) Effects of dietary l-glutamine supplement on performance,
characteristics of the carcase and intestinal morphometry in guinea
fowl chickens (Numida meleagris). Ital J Anim Sci 18(1): 513-521.
Grimm H, Kraus A (2001) Immunonutrition-supplementary amino acids
and fatty acids ameliorate immune deficiency in critically ill patients.
Langenbeck’s Arch Surg 386: 369-376.
Hakim MR, Daryatmo D, Rahardja DP, Pakiding W (2019) Hatching per
formance of indonesian native chicken supplemented by l-glutamine
at different days of incubatio. Chalaza Journal of Animal Husbandry
(1): 1-5.
Holthausen JS, Schregel J, Sciascia QL, Li Z, Tuchscherer A, Vahjen W,
Metges CC, Zentek Z (2022) Effects of oral glutamine supplemen
tation, birthweight and age on colonic morphology and microbiome
development in male suckling piglets. Microorganisms 10(10): 1899
Jacobi SK, Odle J (2012) Nutritional factors influencing intestinal health
of the neonate. Adv Nutr 3: 687-696.
Jazideh F, Farhoomand P, Daneshyar M, Najafi G (2014) The effects of
dietary glutamine supplementation on growth performance and in
testinal morphology of broiler chickens reared under hot conditions.
Turkish J Vet Anim Sci 38: 264-270.
Jia CL, Wei ZH, Yu M, Wang XQ, Yu F (2011) Effect of in-ovo feeding
maltose on the embryo growth and intestine development of broiler
chicken. Indian J Anim Sci 81: 503-506.
Kaminski NA, Wong EA (2018) Differential mRNA expression of nutrient
transporters in male and female chickens. Poult Sci J 97(1): 313-318.
Kanagaraju P, Rathnapraba S (2017) Effect of in-ovo supplementation of
vital nutrients on the production performance and gut histomorphol
ogy of commercial broiler chicken. Int J Livest Res 7(12): 199-205.
Li N, DeMarco VG, West CM, Neu J (2003) Glutamine supports recovery
from loss of transepithelial resistance and increase of permeability in
duced by media change in Caco-2 cells. J Nutr Biochem 14: 401-408.
Li N, Neu J (2009) Glutamine deprivation alters intestinal tight junctions
via a PI3-K/Akt mediated pathway in Caco-2 cells. J Nutr Biochem
: 710-714.
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression
data using real-time quantitative PCR and the 2−ΔΔCT method. Meth
ods 25(4): 402-408.
Ma H, Cahu MC, Zambonino J, Yu H, Duan Q, M-M Le Gall, Mai KS
(2005) Activities of selected digestive enzymes during larval devel
opment of large yellow croaker (Pseudosciaena crocea). Aquac Res
: 239-248.
Noy Y, Sklan D (1997) Posthatch development in poultry. J Appl Poult
Res 6: 344-354.
Ohta Y, Kidd MT (2001) Optimum site for in ovo amino acid injection in
broiler breeder eggs. Poult Sci J, 80: 1425-1429.
Olubodun JO, Zulkifli I, Farjam AS, Hair-Bejo M, Kasim A (2015) Glu
tamine and glutamic acid supplementation enhances performance of
broiler chickens under the hot and humid tropical condition. Ital J
Anim Sci 14: 25-29.
Pedroso AA, Chaves LS, Lopes KLA, Leandro NSM, Café MB, Stringh
ini JH (2006) Inoculação de nutrientes em ovos de matrizes pesadas.
Rev Bras de Zootec 35: 2018-2026.
Potten CS, Booth C, Pritchard DM (1997) The intestinal epithelial stem
cell: the mucosal governor. Int J Exp Pathol 78: 219-243.
Punzo C, Kornacker K, Cepko CL (2008) Stimulation of the insulin/
mTOR pathway delays cone death in a mouse model of retinitis pig
mentosa. Nat Neurosci 12: 44-52.
Retes PL, Clemente AHS, Neves DG, Espósito M, Makiyama L, Alva
renga RR, Pereira LJ, Zangeronimo MG (2018) In ovo feeding of
carbohydrates for broilers-a systematic review. J Anim Physiol Anim
Nutr 00: 1-9.
Rezaei R, Knabe DA, Tekwe CD, Dahanayaka S, Ficken MD, Fielder SE,
Eide SJ, Lovering SL Wu G (2013) Dietary supplementation with
monosodium glutamate is safe and improves growth performance in
postweaning pigs. Amino Acids 44: 911-923.
Rufino JPF, Cruz FGG, Costa VR, Silva AF, Melo LD, Bezerra NS (2019)
Effect of in ovo feeding of l-glutamine to chick embryos. Braz J Poul
try Sci 852: 1-7.
Salmanzadeh M, Ebrahimnezhad Y, Shahryar HA, Ghaleh-Kandi JG
(2016) The effects of in ovo feeding of glutamine in broiler breeder eggs on hatchability, development of the gastrointestinal tract, growth
performance and carcass characteristics of broiler chicken. Arch
Anim Breed 59: 235-242.
Semenza G (1986) Anchoring and biosynthesis of stalked brush border
membrane protein: glycosidases and peptidases of enterocytes and of
renal tubuli. Annu Rev Cell Dev Biol 2: 255-313.
Sentongo T, Mascarenhas MR (2002) Newer components of enteral for
mulas. Pediatr Clin N Am 49: 113-125.
Seth A, Basuroy S, Sheth P, Rao RK (2004) L-glutamine ameliorates acet
aldehyde-induced increase in paracellular permeability in Caco-2 cell
monolayer. Am J Physiol - Gastrointest Liver Physiol 287: 510-517.
Smirnov A, Tako E, Ferket PR, Uni Z (2006) Mucin gene expression and
mucin content in the chicken intestinal goblet cells are affected by in
ovo feeding of carbohydrates. Poult Sci J 85: 669-673.
Sözcü A, Ak İ (2020) Effects of in-ovo injection of glutamine on late
term embryo development, hatchability, one-day old chick quality
and small intestine morphological traits in broilers. Eur Poult Sci 84:
-11.
Tako E, Ferket PR, Uni Z (2004) Effects of in ovo feeding of carbohy
drates and beta-hydroxy-beta-methylbutyrate on the development of
chicken intestine. Poult Sci J 83: 2023-2028.
Uni Z, Ferket PR (2003) Enhancement of development of oviparous spe
cies by in ovo feeding. US patent number 6,592,878.
Uni Z, Ferket PR, Tako E, Kedar O (2005) In ovo feeding improves en
ergy status of late-term chicken embryos. Poult Sci J 84: 764-770.
Uni Z, Tako E, Gal-Garber O, Sklan D (2003) Morphological, molecular,
and functional changes in the chicken small intestine of the late-term
embryo. Poult Sci J 82: 1747-1754.
Uni Z, Yahav S (2010) Managing prenatal development of broiler chick
ens to improve productivity and thermotolerance. In: Managing The
Prenatal Environment to Enhance Livestock Productivity. Ed. Paul L.
Greenwood, Alan W. Bell, Philip E. Vercoe, Gerrit J. Viljoen. Spring
er Dordrecht Heidelberg London New York.
Wang B, Wu G, Zhou Z, Dai Z, Sun Y, Ji Y, Li W, Wang W, Liu C, Han F,
Wu Z (2014) Glutamine and intestinal barrier function. Amino Acids
: 2143-2154.
Wang J, Chen L, Li P, Li X, Zhou H, Wang F, Li D, Yin Y, Wu G (2008)
Gene expression is altered in piglet small intestine by weaning and
dietary glutamine supplementation. J Nutr 138: 1025-1032.
Willing BP, Van Kessel AG (2007) Enterocyte proliferation and apoptosis
in the caudal small intestine is influenced by the composition of col
onizing commensal bacteria in the neonatal gnotobiotic pig. J Anim
Sci 85: 3256-3266.
Wu G, Meier SA, Knabe DA (1996) Dietary glutamine supplementation
prevents jejunal atrophy in weaned pigs. J Nutr 126: 2578-2584.
Wu Q, Wang C, Liao J, Hu N, Cheng B, Ma Y, Wang Y (2022) Effects of
dietary supplementation with glutamine on the immunity and intesti
nal barrier gene expression in broiler chickens infected with salmo
nella enteritidis. Animals 12(17): 2168-2185.
Wu QJ, Liu ZH, Jiao C, Cheng BY, Zhu DD, Ma Y, Wang YQ, Ma WF,
Wang Y (2020) Effects of glutamine on growth performance, intesti
nal morphology and intestinal barrier function of broilers. Indian J.
Anim. Res 1-6.
Yair R, Uni Z (2011) Content and uptake of minerals in the yolk of broiler
embryos during incubation and effect of nutrient enrichment. Poult
Sci J 90: 1523-1531.
Yan L, Qiu-Zhou X (2006) Dietary glutamine supplementation improves
structure and function of intestine of juvenile Jian carp (Cyprinus
carpio var. Jian.) Aquac 256: 389-394.
Youssef SF, Abd El-Halim HAH, Wahba FA, Abd El-Maged MH (2017)
Effect of in ovomethionine, glutamine, carnitine or betaine injectıon
on hatchability, growth performance and physiologıcal state. Egypt
Poult Sci J 37(4): 1273-1287.
Zhai W, Gerard PD, Pulikanti R, Peebles ED (2011a) Effects of in ovo in
jection of carbohydrates on embryonic metabolism, hatchability, and
subsequent somatic characteristics of broiler hatchlings. Poult Sci J
: 2134-2143.
Zhai W, Rowe DE, Peebles ED (2011b) Effects of commercial in ovo in
jection of carbohydrates on broiler embryogenesis. Poult Sci J 90:
-1301.