Concentrations of plasma estradiol, progesterone, melatonin, serotonin and nitric oxide in Tuj ewes after estrus syncronization in and out of breeding season

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Опубликован: Ιουλ 9, 2024
DOI: https://doi.org/10.12681/jhvms.31624
C Okulmus
1Department of Biochemistry, Izmir/Bornova Veterinary Control Institute, Izmir, Turkey
E Atakisi
Department of Biochemistry, Faculty of Veterinary Medicine, Kafkas University, Kars, Turkey
Аннотация

This study aimed to determine and compare the concentrations of progesterone, estradiol, melatonin, serotonin and nitric oxide in the blood plasma following induced estrus in (autumn) and outside (spring) the natural breeding season in ewes. In addition, seasonal changes in serotonin levels following induced estrus in ewes were examined for the first time. A total of 20 Tuj ewes were used in the study. The ewes were synchronized in the breeding season (n=10) and non-breeding season (n=10) by using an intravaginal progestin-containing sponge (60 mg medroxy-progesterone acetate) for 12 days. Then, the sponge was withdrawn, and 2.5 mL of prostaglandin F2 alpha (PGF2α) was injected intramuscularly with 500 IU pregnant mare serum gonadotrophin (PMSG). The first blood samples were collected immediately after removal of the intravaginal sponge (at 0 hour) and the rest was collected at 4-hour intervals between 24 and 68 hours.


Throughout both seasons, all animals displayed estrus. However, estrus appeared sooner and was more intense in the breeding season than in the non-breeding season. Plasma concentrations of progesterone (respectively; 0.65±0.36 vs. 0.47±0.20 ng/mL, P < 0.05), estradiol (respectively; 35.42±26.66 vs. 28.36±19.86 ng/L, P < 0.05), nitric oxide (respectively 15.01±2.32 vs. 14.41±2.74 µmol/L, P < 0.05) and melatonin (respectively; 118.76 ±35.5 vs. 93.03±44.34 ng/L, P < 0.05) were higher in the breeding season and a positive correlation was recorded between estradiol and changes in progesterone (r=0.685; P < 0.05) and nitric oxide (r=0.535; P < 0.05). However, the plasma serotonin concentrations (respectively; 66.16 ±21.93 vs. 50.79±29.18 ng/mL, P < 0.05) were higher in the non-breeding season and showed a positive correlation with melatonin (r=0.671; P < 0.05). In addition, significant differences were noted between day and night concentration changes in melatonin and serotonin hormones in both seasons (P < 0.05). In conclusion, the results show seasonal differences in the secretion of ovarian steroid hormones and nitric oxide following induced estrus, which suggests a different reproduction capacity in the two seasons. Seasonal differences in the profile of nitric oxide release during estrus are likely related to estradiol secretion. The study shows that changes in melatonin and serotonin concentrations and circadian differences in their release in and out the breeding season are not affected by estrus synchronization.

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Библиографические ссылки
Abecia JA, Forcada F, González-Bulnes A, (2012) Hormonal control of
reproduction in small ruminants. Anim. Reprod. Sci. 130: 173-179.
Battista PJ, Condon WA (1986) Serotonin-induced stimulation of progesterone production by cow luteal cells in vitro. J. Reprod. Fert. 76:
-238.
Blache D, Fabre-Nys C, Venier G (1996) Inhibition of sexual behaviour
and the luteinizing hormone surge by intracerebral progesterone implants in the female sheep. Brain. Res, 741(1-2): 117-122.
Brunet AG, Moreno JS, Campo AD, Malpaux B, Chemineau P, Tortonese
DJ, Sebastian AL, Bulnes AG (2008) Endogenous circannual cycles
of ovarian activity and changes in prolactin and melatonin secretion
in wild and domestic female sheep maintained under a long-day photoperiod. Bio. of Reprod. 78: 552-562.
Callaghan DO (1999) A Practical approach management of reproductive
seasonality in sheep. Faculty of Veterinary Medicine, University College Dublin, Ballsbridge, Dublin, Ireland. Reprod. Domes. Animal.
: 285-291.
Cevik M, Yurdaydın N (2001) Effect of photoperiodism on fertility in domestic Animals. Livestock Studies. 38 (1): 69-78.
Chemineau P, Malpaux B, Delgadillo JA, Guerin Y, Ravault JP, Thimonier
J, Pelletier J. (1992) Control of sheep and goat reproduction: use of
light and melatonin. Anim. Reprod. Sci. 30: 157-184.
Coelho LA, Rodrigues PA, Nonaka KO (2006) Annual pattern of plasma melatonin and progesterone concentrations in hair and wool ewe
lambs kept under natural photoperiod at lower latitudes in the southern hemisphere. J. Pineal Res. 41(2): 101-107.
Dixit VD, Parvizi N (2001) Nitric oxide and the control of reproduction.
Anim. Reprod. Sci. 65: 1-16.
Ekiz EE, Ozcan M (2006) Sexual behavior and hormone levels of Kıvırcık
ewes after estrus synchronization during and out of the breeding season. Arch. Tierz., Dummerstorf. 49 (6): 583-592.
Emrelli AZ, Horoz H, Tek C (2003) The effect of melatonin and progestagen treatments on stimulation of oestrus cycle and reproductive patterns in Merino ewes out of the breeding season. Acta.
Vet. Eurasia. 29(2): 267-275.
Forcada F, Abecia JA, (2006) The effect of nutrition on the seasonality of
reproduction in ewes. Reprod. Nutr. Dev. 46: 355-365.
Goda R, Otsuka T, Iwamoto A, Kawai M, Shibata S, Furuse M, Yasuo S
(2015) Serotonin levels in the dorsal raphe nuclei of both chipmunks
and mice are enhanced by long photoperiod, but brain dopamine level response to photoperiod is species-specific. Neuroscience Letters.
: 95-100.
Gordon I (1997) Controlled reproduction in sheep and goats. 1st ed., s.
-434. CAB International, New York.
Hashemi M, Safdarian M, Kafi M (2006) Estrus response to synchronization of estrus using different progesterone treatments outside the
natural breeding season in ewes. Small Rumin. Res. 65(3): 279- 283.
Ileri IK, Horoz H, Ak K, Senünver A (1996) Studies on the determination
of progesterone levels by radioimmunoassay method and early pregnancy diagnosis in Kıvırcık Sheep. J of Anim. Res. 6 (1-2): 61-63.
Kaya S, Kacar C, Kaya D, Aslan S (2013) The effectiveness of supplemental administration of progesterone with GnRH, hCG and PGF2α
on the fertility of Tuj sheep during the non-breeding season. Small
Rumin. Res. 113: 365-370.
Khan FA, Scholtz EL, Chenier TS (2015) The nitric oxide system in
equine reproduction: Current Status and Future Directions. J. Equine
Vet. Sci. 35(6): 481-487.
Kumar S, Purohit GN (2009) Effect of a single subcutaneous injection of
melatonin on estrous response and conception rate in goats. Small
Rum. Res. 82: 152-155.
Le Corre S, Chemineau P (1993) Control of photoperiodic inhibition of
luteinizing hormone secretion by dopaminergic and serotonergic systems in ovariectomized Ile-de-France ewes supplemented with oestradiol. J. Reprod. and Fertility. 97: 367-373.
Leyva V, Bucluell BC, Walton JS (1998) Follicular activity and ovulation
regulated by exogenous progestagen and PMSG in anestrous ewes.
Theriogenology. 50: 377-393.
Luridiana S, Mura MC, Daga C, Farci F, Di Stefano MV, Zidda F
(2015) Melatonin treatment in spring and reproductive recovery in
sheep with different body condition score and age. Animal Reprod.
Sci. 160: 68-73.
Menegatos J, Chadio S, Kalogiannis T, Kouskoura T, Kouimtzis S (2002)
Endocrine events during the periestrous period and the subsequent
estrous cycle in ewes after estrus synchronization. Theriogenology.
: 1-11.
Miranda KM, Espey MG, Wink DA (2001) A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric
Oxide - Bio Chem. 5(1): 62-71.
Misztal T, Romanowicz K, Barcikowski B (1996) Seasonal changes of
melatonin secretion in relation to the reproductive cycle in sheep. J
Anim. Feed Sci. 5: 35-48.
Mondragon AM, Garverick HA, Moiler TA, Elmore RG (1986) Effect of
serotonin on LH secretory patterns in ovarıectomized cows. Domest.
Anim. Endoc. 3(3): 145-151.
Mura MC, Luridiana S, Farci F, Di Stefano MV, Daga C, Pulinas L, Staric
J, Carcangiu V (2017) Melatonin treatment in winter and spring and
reproductive recovery in Sarda breed sheep. Anim. Reprod. Sci, 185:
-108.
Mura MC, Luridiana S, Pulinas L, Bizarri D, Cosso G, Carcangiu V
(2019) Melatonin treatment and male replacement every week on
the reproductive performance in Sarda sheep breed. Theriogenology.
: 80-84.
Nath P, Maitra S (2018) Physiological relevance of nitric oxide in ovarian
functions: an overview. Gen. Comp. Endocrinol. 35: 279.
Olivier JDA, Esquivel-Franco DC, Waldinger MD, Olivier B (2019) Serotonin and sexual behavior. In: The Serotonin System: Academic
Press. 753: 2-18.
Oral H, Ogun M, Kuru M, Kaya S (2015) Evaluation of certain oxidative
stress parameters in heifers that were administered short term Prıd.
Kafkas Univ Vet Fak Derg. 21 (4): 569-573.
Payne JH, Vaillant C, Cooke RG (1986) Serotonin as a possible physiological regulator of ovine luteal function. Anim. Reprod. Sci. 36(1-2):
-103.
Piccione G, Giannetto C, Assenza A, Fazio F, Caola G (2008) Locomotor
activity and serum tryptophan and serotonin in goats: daily rhythm. J
Appl Biomed. 6: 73-79.
Powell MR, Kaps M, Lamberson WR, Keisler DH (1996) Use of melengestrol acetate-based treatments to induce and synchronize estrus
in seasonally anestrous ewes. J. of Anim. Sci. 74(10): 2292-2302.
Quay WB (1963) Circadian rhythm in rot pineal serotonin and its modifications by estrous cycle and photoperiod. Gen. Comp. Endoc. 3:
-479.
Ritar AJ, Maxwell WMC, Salamon S (1984) Ovulation and LH secretion
in the goat after intravaginal progestagen sponge-PMSG treatment. J.
Reprod. Fertil. 72: 559-563.
Ritar AJ, Robertson JA, Evans G (1994) Ovulatory activity, hormonal
induction of ovulation and fertility of young Cashmere and Angora
female goats in a temperate environment. Reprod. Fertil. Dev. 6: 737-
Romano JE, Christians CJ, Crabo BG (2000) Continuous presence of rams
hastens the onset of estrus in ewes synchronized during the breeding.
Appl. Anim. Behav. Sci. 66: 65-70.
Rosselli M, Keller PJ, Dubey RK (1998) Role of nitric oxide in the biology, physiology and pathophysiology of reproduction. Hum. Reprod.
Update 4: 3-24.
Saatci M, Yıldız S, Kaya I (2003) New rearing systems for Tuj (Tushin)
lambs. Small Rumin. Res. 50: 23-27.
Sarrias MJ, Artigas F, Martinez E, Gelpi E (1989) Seasonal changes of
plasma serotonin and related parameters: correlation with environmental measures. Biol Psychiatry. 26: 695-706.
Swellum A, Alowaimer A, Abouheif M (2015) Use of fluorogestone acetate sponges or controlled internal drug release for estrus synchronization in ewes: Effects of hormonal profiles and reproductive performance. Theriogenology. 84(4): 498-503.
Tekin ME (2003) Biostatistics on computer with examples for health sciences (Veterinary, Medicine, Dentistry, Pharmacy). Selçuk Univ. Vet.
Fak. Publishing Unit. Konya, TURKEY.
Todini L, Terzano GM, Borghese A, Debenedetti A, Malfatti A (2011)
Plasma melatonin in domestic female Mediterranean sheep (Comisana breed) and goats (Maltese and Red Syrian). Res Vet Sci. 90: 35-39.
Van Cleeff J, Karsch FJ, Padmanabhan V (1998) Characterization of endocrine events during the periestrous period in sheep after estrous
synchronization with controlled internal drug release (CIDR) device.
Domest. Anim. Endocrinol. 15: 23-34.
van Werven T, Waldeck F, Souza AH, Floch S, Englebienne M (2013)
Comparison of two intravaginal progesterone releasing devices
(PRID-Delta vs CIDR) in dairy cows: Blood progesterone profile and
field fertility. Anim. Reprod. Sci. 138(3-4): 143-149.
Veille JC, Li P, Eisenach JC, Massmann AG, Massmann AG, Figueroa JP
(1996) Effects of estrogen on nitric oxide biosynthesis and vasorelaxant activity in sheep uterine and renal arteries in vitro. Am. J Obstet.
Gynec. 174(3): 1043-1049.
Vifloles C, Forsberg M, Banchero G, Rubianes E (2001) Effect of longterm and short- term progestagen treatment on follicular development
and pregnancy rate in cyclic ewes. Theriogenology. 55: 993-1004.
Whisnant CS, Goodman RL (1990) Further evidence that serotonin mediates the steroid ındependent ınhibition of luteinizing hormone secretion in anestrous ewes. Bio. of Reprod. 42, 656-661.
Willams LM Helliwell RJA (1993) Melatonin and Seosonality in the
Sheep. Anim. Reprod. Sci. 33: 159- 182
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