Development of real-time qPCR assays for detecting and quantifying common bacterial pathogens in fish from Mediterranean aquacultures

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Publiée : Nov 12, 2024
DOI : https://doi.org/10.12681/mms.38053
EVANGELIA D. APOSTOLIDI
Veterinary Research Institute, Hellenic Agricultural Organization DIMITRA, 57001 Thermi, Thessaloniki, Greece
KONSTANTINA BITCHAVA
School of Animal Biosciences, Agricultural University of Athens, 11855, Athens, Greece
ELENI TSAVEA
Veterinary Research Institute, Hellenic Agricultural Organization DIMITRA, 57001 Thermi, Thessaloniki, Greece
ILIAS BOUZALAS
Veterinary Research Institute, Hellenic Agricultural Organization DIMITRA, 57001 Thermi, Thessaloniki, Greece
CHRYSOULA GUBILI
Fisheries Research Institute, Hellenic Agricultural Organization DIMITRA, 64007 Nea Peramos, Kavala, Greece
GRIGORIOS KREY
Fisheries Research Institute, Hellenic Agricultural Organization DIMITRA, 64007 Nea Peramos, Kavala, Greece
EVRIDIKI BOUKOUVALA
Veterinary Research Institute, Hellenic Agricultural Organization DIMITRA, 57001 Thermi, Thessaloniki, Greece
Résumé

The aquaculture industry is a rapidly developing and economically important sector for many countries, thus it requires effective solutions to overcome the key challenges that could impede its growth and sustainability. Among the most serious concerns are fish diseases caused by bacterial infections. This study introduces the development of novel rapid molecular methods employing Real-Time Polymerase Chain Reaction (qPCR) for the specific detection and quantification of five major fish pathogenic bacteria: Vibrio harveyi, V. alginolyticus, V. anguillarum, Photobacterium damselae, and Tenacibaculum maritimum. These bacteria are responsible for diseases such as vibriosis, photobacteriosis, and tenacibaculosis. The qPCR assays developed in this study are highly specific and extremely sensitive, making them suitable for early detection of these pathogens, thus aiding in the prevention of disease outbreaks in aquaculture farms.

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