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Differentiation of leptospiral serovars by restriction endonucleases analysis

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Ι. Μ. ELJALII, A. S. ALI
Ι. Μ. ELJALII, A. S. ALI

Abstract


DNA from ten leptospiral field isolates was digested with restriction enzymes, Hindlll, BamHl and EcoRl to differentiate leptospiral serovars. A high heterogeneity among the serovars examined was revealed. Similarities were observed among isolates of the same serovar. No relationship was found between the restriction patterns and the species from which the field strain was isolated. It appears that restriction endonucleases analysis would be useful in the differentiation of the leptospiral serovars.


Keywords


Leptospira; serovars; restriction enzymes

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References


Bolin, C. A and Zuerner, R. L. (1996). Correlation between DNA restriction fragment length polymorphisms in Leptospira interrogans serovar pomona type kennewicki and host animal source. J Clin Microbiol. 34:424-425.

Boqvist, S., Montgomery, JM., Hurst, M., Thu, H.T., Engvall, E.O., Gunnarsson, A and Magnusson, U.( 2003). Leptospira in slaughtered fattening pigs in southern Vietnam: presence of the bacteria in the kidneys and association with morphological findings. Vet Microbiol. 93:361-368.

Brenner, D. J., Kaufmann, Α. F., Sulzer, Κ. R., Steigerwalt, A. G., Rogers, F. C and Weyant, R. S. (1999). Further determination of DNA relatedness between serogroups and serovars in the family Leptospiraceae with a proposal for Leptospira alexanderi sp. nov. and four new Leptospira genomospecies. Int J Syst Bacteriol. 49 :839-858.

Brown, P. D. and Levett, P. N. (1997). Differentiation of leptospira species and serovars by PCR, restriction endonuclease analysis, arbitrary primed PCR and low stringency PCR. Journal of Medical Microbiology. 46: 173-181.

Corney, B. G. and Colley, J. (1996). Restriction fragment length polymorphism in Australian and New Zealand isolates of Leptospira interrogans serovar pomona. Australian Veterinary Journal. 74: 156-157.

Djordjevic, S., Hornitzky, M., Ross, A. D. and Whittington, R. J. (1993). Restriction-endonuclease analysis of Australian isolates of Leptospira interrogans serovar hardjo from cattle with agalactia and abortion. Australian Veterinary Journal. 70: 98-100.

Ellis, W.A., Thiermann, A.B., Montgomery, J., Handsaker, Α., Winter, P.J. and Marshall, R.B. (1988). Restriction endonuclease analysis of Leptospira interrogansserovar hardjo isolates from cattle. Res Vet Sci.443:375-379.

Ellis, W.A.,Montgomery, J.M. and Thiermann, A.B. (1991). Restriction endonuclease analysis as a taxonomic tool in the study of pig isolates belonging to the Australis serogroup of Leptospira interrogans. J Clin Microbiol. 29:957-961.

Feresu, S. B., Steigerwalt, A. G. and Brenner, D. J. (1999). DNA relatedness of Leptospira strains isolated from beef cattle in Zimbabwe. Int J Syst Bacteriol. 49:1111-1117.

Garrity, G.M. and Holt, J.G. (2001). The road map to the manual. Bergey's Manual of Systematic Bacteriology. 2nd edition. Edited by: Boone and Castenholz RW. Berlin, pringer-Verlag.

Hathaway, S.C., Marshall, R.B., Little, T. W.A., Headlam, S.A. and Winter, P.J. (1985). Differentiation of reference strains of leptospires of the Pomona serogroup by cross-agglutination and restriction endonuclease analysis. Research in Veterinary Science. 39: 145-150.

Khairani, B. (1997). A study of the leptospiral isolates obtained from cattle in Malaysia. Master Thesis, University Pertanian Malaysia.

Letocard, M., Baranton, G. and Perolat, P. (1997). Rapid identification of pathogenic Leptospira species (Leptospira interrogans, L. borgpetersenii and L. kirschneri) with species-specific DNA probe produced by arbitrary primed PCR. Journal of Clinical Microbiology. 35: 248-253.

Levett, P.N.( 2001). Leptospirosis. Clin Microbiol Rev. 14:296-326. Marshall, R.B., Wilton, B.E. and Robinson, A.J. (1981). Identification of Leptospira serovars by restriction endonuclease analysis. Journal of Medical Microbiology. 14: 163-166.

Marshall, R.B.,Winter, P.J. and Yanagawa, R. (1984). Restriction endonuclease DNA analysis of Leptospira interrogans serovars icterohaemorrhagiae and hebdomadis. J Clin Microbiol. 20:808-

Pacciarini, M.L., Savio, M.L., Tagliabue, S. and Rossi, C (1992). Repetitive sequences cloned from Leptospira interrogans serovar harjdo genotype hardjoprajitno and their application to serovar identification. Journal of Clinical Microbiology. 30: 1243-1249.

Perolat, P., Chappel, R.J., Adler, Β., Baranton, G., Bulach, D.M., Billinghurst, M.L., Letocart, M., Merien, F. and Serrano, M.S. (1998). Leptospira fainei sp. nov., isolated from pigs in Australia. Int J Syst Bacteriol.. 48 :851-858.

Ramadass, P., Jarvis, B.D., Corner, R. J., Penny, D. and Marshall, R. B. (1992). Genetic characterization of pathogenic Leptospira species by DNA hybridization. Int J Syst Bacteriol. 42: 215-219.

Robinson, A.J., Ramadass, P., Lee, A. and Marshall, R.B. (1982). Differentiation of subtypes within Leptospira interrogans serovars hardjo, balcanica and tarassovi by bacterial restriction endonuclease analysis (BRENDA). Journal of Medical Microbiology. 15: 331-338.

Rocha, T., Ellis, W.A., Montgomery, J., Gilmore, C, Regalla, J. and Brem, S. (2004). Microbiological and serological study of leptospirosis in horses at slaughter: first isolations. Research Veterinary Science. 76:199-202.

Savio, M.L., Rossi, C, Fusi, P., Tagliabue, S. and Pacciarini, M.L. (1994). Detection and identification of Leptospira interrogans serovars by PCR coupled with restriction endonuclease analysis of amplified DNA. Journal of Clinical Microbiology.32: 935-941.

Shi, M., Liang, Z., Du, X., Jiang, X. and Nie, Y. (2000). A molecular epidemiological investigation on Leptospira interrogans serovar hebdomadis and australia in China. Zhonghua Liu Xing Bing Xue Za Zhi. 21:91-93.

Silbreck, Ν. W. and Davis, W. D. (1989). Restriction endonuclease analysis of Australian isolates of Leptospira interrogans serovar hardjo. Australian Veterinary Journal. 66: 183-184.

Tamai, T., Sada, E. and Kobayashi, Y. (1988). Restriction endonuclease DNA analysis of Leptospira interrogans serovars Icterohaemorrhagiae and Copenhageni. Microbiol Immunol.; 32(9):887-94.

Terpstra, W.J., Korver, H., Schoone, G.J., von Leeuwen, J., Schonemann, CE., de Jonge-Aglibut, S. and Kolk, A.H. (1987). Comparative classification of Leptospira serovars of the Pomona group by monoclonal antibodies and restriction-endonuclease analysis. Zentralbl Bakteriol Mikrobiol Hyg. 266:412-421.

Thiermann, A.B. and Le Vebvre, R.B. (1988). Restriction Endonuclease analysis of leptospiral pathogens from North America. Israel Journal of Veterinary Medicine. 44: 62.

Thiermann, A. B., Handsaker, A. L., Moseley, S.L. and Kingscote, B. (1985). New method for classification of leptospiral isolates belonging to serogroup pomona by restriction endonuclease analysis: serovar kennewicki. J Clin Microbiol. 21:585-587

Thiermann, A.B., Handsaker, A. L., Foley, J. W., White, F. H. and Kingscote, B. F. (1986). Reclassification of North American leptospiral isolates belonging to serogroups Mini and Sejroe by restriction endonuclease analysis. American Journal of Veterinary Research. 47: 61-66.

Woodward, M.J. and Redstone, J.S. (1993). Differentiation of Leptospira serovars by polymerase chain reaction and restriction fragment length polymorphism. Veterinary Record. 132: 325-326.

Yasuda, P.H., Steigerwalt, A.G., Sulzer, K.R., Kaufmann, A.F., Rogers, F. and Brenner, D.J. (1987). Deoxyribonucleic acid relatedness between serogroups and serovars in the family Leptospiraceae with proposals for seven new Leptospira species.Int. J. Syst. Bacteriol: 1987 - 407-415.

Zuerner, R.L. and Bolin, CA. (1990). Nucleic acid probe characterizes leptospira interrogans serovars by restriction fragment length polymorphism. Veterinary Microbiology. 24: 355-365.

Zuerner, R.L., Ellis, W.A., Bolin, CA. and Montgomery, J.M. (1993). Restriction fragment length polymorphisms distinguish Leptospira borgpetersenii serovar hardjo type hardjo-bovis isolates from different geographical locations. J Clin Microbiol. 31:578-83


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