Διαφοροποίηση των στελεχών λεπτόσπειρας με περιοριστικά ένζυμα

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Δημοσιευμένα: Nov 29, 2017
DOI: https://doi.org/10.12681/jhvms.15060
Λέξεις-κλειδιά:
Leptospira serovars restriction enzymes
Ι. Μ. ELJALII
Department of Preventive Medicine and Public Health, Faculty of Veterinary Medicine, University of Khartoum
A. S. ALI
Department of Preventive Medicine and Public Health, Faculty of Veterinary Medicine, University of Khartoum
Περίληψη

DNA από δέκα στελέχη λεπτόσπειρας χρησιμοποιήθηκε για πέψη με τα ένζυμα περιορισμού HindUl, BamHi και  EcoEl, με σκοπό τη διαφοροποίηση τους ανά ορότυπο. Μεταξύ των ορότυπων που εξετάστηκαν διαπιστώθηκε υψηλή ετερογένεια. Ομοιότητες αναδείχθηκαν μεταξύ στελεχών του ίδιου ορότυπου. Δεν διαπιστώθηκε συσχέτιση μεταξύ του είδους από το οποίο απομονώθηκαν τα στελέχη που εξετάστηκαν και των τύπων στους οποίους διακρίθηκαν βάσει τυποποίησης με ένζυμα περιορισμού. Φαίνεται ότι η ανάλυση με τη χρήση ένζυμων περιορισμού θα ήταν χρήσιμη στη διάκριση ορότυπων της λεπτόσπειρας.

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Λήψεις
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