Differentiation of leptospiral serovars by restriction endonucleases analysis

PDF (English)
Pubblicato: Nov 29, 2017
DOI: https://doi.org/10.12681/jhvms.15060
Ι. Μ. ELJALII
Department of Preventive Medicine and Public Health, Faculty of Veterinary Medicine, University of Khartoum
A. S. ALI
Department of Preventive Medicine and Public Health, Faculty of Veterinary Medicine, University of Khartoum
Abstract

DNA from ten leptospiral field isolates was digested with restriction enzymes, Hindlll, BamHl and EcoRl to differentiate leptospiral serovars. A high heterogeneity among the serovars examined was revealed. Similarities were observed among isolates of the same serovar. No relationship was found between the restriction patterns and the species from which the field strain was isolated. It appears that restriction endonucleases analysis would be useful in the differentiation of the leptospiral serovars.

Article Details
  • Sezione
  • Research Articles

Authors who publish with this journal agree to the following terms:

· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Downloads
I dati di download non sono ancora disponibili.
Riferimenti bibliografici
Bolin, C. A and Zuerner, R. L. (1996). Correlation between DNA restriction fragment length polymorphisms in Leptospira interrogans serovar pomona type kennewicki and host animal source. J Clin Microbiol. 34:424-425.
Boqvist, S., Montgomery, JM., Hurst, M., Thu, H.T., Engvall, E.O., Gunnarsson, A and Magnusson, U.( 2003). Leptospira in slaughtered fattening pigs in southern Vietnam: presence of the bacteria in the kidneys and association with morphological findings. Vet Microbiol. 93:361-368.
Brenner, D. J., Kaufmann, Α. F., Sulzer, Κ. R., Steigerwalt, A. G., Rogers, F. C and Weyant, R. S. (1999). Further determination of DNA relatedness between serogroups and serovars in the family Leptospiraceae with a proposal for Leptospira alexanderi sp. nov. and four new Leptospira genomospecies. Int J Syst Bacteriol. 49 :839-858.
Brown, P. D. and Levett, P. N. (1997). Differentiation of leptospira species and serovars by PCR, restriction endonuclease analysis, arbitrary primed PCR and low stringency PCR. Journal of Medical Microbiology. 46: 173-181.
Corney, B. G. and Colley, J. (1996). Restriction fragment length polymorphism in Australian and New Zealand isolates of Leptospira interrogans serovar pomona. Australian Veterinary Journal. 74: 156-157.
Djordjevic, S., Hornitzky, M., Ross, A. D. and Whittington, R. J. (1993). Restriction-endonuclease analysis of Australian isolates of Leptospira interrogans serovar hardjo from cattle with agalactia and abortion. Australian Veterinary Journal. 70: 98-100.
Ellis, W.A., Thiermann, A.B., Montgomery, J., Handsaker, Α., Winter, P.J. and Marshall, R.B. (1988). Restriction endonuclease analysis of Leptospira interrogansserovar hardjo isolates from cattle. Res Vet Sci.443:375-379.
Ellis, W.A.,Montgomery, J.M. and Thiermann, A.B. (1991). Restriction endonuclease analysis as a taxonomic tool in the study of pig isolates belonging to the Australis serogroup of Leptospira interrogans. J Clin Microbiol. 29:957-961.
Feresu, S. B., Steigerwalt, A. G. and Brenner, D. J. (1999). DNA relatedness of Leptospira strains isolated from beef cattle in Zimbabwe. Int J Syst Bacteriol. 49:1111-1117.
Garrity, G.M. and Holt, J.G. (2001). The road map to the manual. Bergey's Manual of Systematic Bacteriology. 2nd edition. Edited by: Boone and Castenholz RW. Berlin, pringer-Verlag.
Hathaway, S.C., Marshall, R.B., Little, T. W.A., Headlam, S.A. and Winter, P.J. (1985). Differentiation of reference strains of leptospires of the Pomona serogroup by cross-agglutination and restriction endonuclease analysis. Research in Veterinary Science. 39: 145-150.
Khairani, B. (1997). A study of the leptospiral isolates obtained from cattle in Malaysia. Master Thesis, University Pertanian Malaysia.
Letocard, M., Baranton, G. and Perolat, P. (1997). Rapid identification of pathogenic Leptospira species (Leptospira interrogans, L. borgpetersenii and L. kirschneri) with species-specific DNA probe produced by arbitrary primed PCR. Journal of Clinical Microbiology. 35: 248-253.
Levett, P.N.( 2001). Leptospirosis. Clin Microbiol Rev. 14:296-326. Marshall, R.B., Wilton, B.E. and Robinson, A.J. (1981). Identification of Leptospira serovars by restriction endonuclease analysis. Journal of Medical Microbiology. 14: 163-166.
Marshall, R.B.,Winter, P.J. and Yanagawa, R. (1984). Restriction endonuclease DNA analysis of Leptospira interrogans serovars icterohaemorrhagiae and hebdomadis. J Clin Microbiol. 20:808-
Pacciarini, M.L., Savio, M.L., Tagliabue, S. and Rossi, C (1992). Repetitive sequences cloned from Leptospira interrogans serovar harjdo genotype hardjoprajitno and their application to serovar identification. Journal of Clinical Microbiology. 30: 1243-1249.
Perolat, P., Chappel, R.J., Adler, Β., Baranton, G., Bulach, D.M., Billinghurst, M.L., Letocart, M., Merien, F. and Serrano, M.S. (1998). Leptospira fainei sp. nov., isolated from pigs in Australia. Int J Syst Bacteriol.. 48 :851-858.
Ramadass, P., Jarvis, B.D., Corner, R. J., Penny, D. and Marshall, R. B. (1992). Genetic characterization of pathogenic Leptospira species by DNA hybridization. Int J Syst Bacteriol. 42: 215-219.
Robinson, A.J., Ramadass, P., Lee, A. and Marshall, R.B. (1982). Differentiation of subtypes within Leptospira interrogans serovars hardjo, balcanica and tarassovi by bacterial restriction endonuclease analysis (BRENDA). Journal of Medical Microbiology. 15: 331-338.
Rocha, T., Ellis, W.A., Montgomery, J., Gilmore, C, Regalla, J. and Brem, S. (2004). Microbiological and serological study of leptospirosis in horses at slaughter: first isolations. Research Veterinary Science. 76:199-202.
Savio, M.L., Rossi, C, Fusi, P., Tagliabue, S. and Pacciarini, M.L. (1994). Detection and identification of Leptospira interrogans serovars by PCR coupled with restriction endonuclease analysis of amplified DNA. Journal of Clinical Microbiology.32: 935-941.
Shi, M., Liang, Z., Du, X., Jiang, X. and Nie, Y. (2000). A molecular epidemiological investigation on Leptospira interrogans serovar hebdomadis and australia in China. Zhonghua Liu Xing Bing Xue Za Zhi. 21:91-93.
Silbreck, Ν. W. and Davis, W. D. (1989). Restriction endonuclease analysis of Australian isolates of Leptospira interrogans serovar hardjo. Australian Veterinary Journal. 66: 183-184.
Tamai, T., Sada, E. and Kobayashi, Y. (1988). Restriction endonuclease DNA analysis of Leptospira interrogans serovars Icterohaemorrhagiae and Copenhageni. Microbiol Immunol.; 32(9):887-94.
Terpstra, W.J., Korver, H., Schoone, G.J., von Leeuwen, J., Schonemann, CE., de Jonge-Aglibut, S. and Kolk, A.H. (1987). Comparative classification of Leptospira serovars of the Pomona group by monoclonal antibodies and restriction-endonuclease analysis. Zentralbl Bakteriol Mikrobiol Hyg. 266:412-421.
Thiermann, A.B. and Le Vebvre, R.B. (1988). Restriction Endonuclease analysis of leptospiral pathogens from North America. Israel Journal of Veterinary Medicine. 44: 62.
Thiermann, A. B., Handsaker, A. L., Moseley, S.L. and Kingscote, B. (1985). New method for classification of leptospiral isolates belonging to serogroup pomona by restriction endonuclease analysis: serovar kennewicki. J Clin Microbiol. 21:585-587
Thiermann, A.B., Handsaker, A. L., Foley, J. W., White, F. H. and Kingscote, B. F. (1986). Reclassification of North American leptospiral isolates belonging to serogroups Mini and Sejroe by restriction endonuclease analysis. American Journal of Veterinary Research. 47: 61-66.
Woodward, M.J. and Redstone, J.S. (1993). Differentiation of Leptospira serovars by polymerase chain reaction and restriction fragment length polymorphism. Veterinary Record. 132: 325-326.
Yasuda, P.H., Steigerwalt, A.G., Sulzer, K.R., Kaufmann, A.F., Rogers, F. and Brenner, D.J. (1987). Deoxyribonucleic acid relatedness between serogroups and serovars in the family Leptospiraceae with proposals for seven new Leptospira species.Int. J. Syst. Bacteriol: 1987 - 407-415.
Zuerner, R.L. and Bolin, CA. (1990). Nucleic acid probe characterizes leptospira interrogans serovars by restriction fragment length polymorphism. Veterinary Microbiology. 24: 355-365.
Zuerner, R.L., Ellis, W.A., Bolin, CA. and Montgomery, J.M. (1993). Restriction fragment length polymorphisms distinguish Leptospira borgpetersenii serovar hardjo type hardjo-bovis isolates from different geographical locations. J Clin Microbiol. 31:578-83