Flow cytometry and its applications

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Published: Nov 29, 2017
DOI: https://doi.org/10.12681/jhvms.15073
Keywords:
flow cytometry method applications
E. I. PAPADOGIANNAKIS (E. Ι. ΠΑΠΑΔΟΓΙΑΝΝΑΚΗΣ)
Dept. of Veterinary Public Health, National School of Public Health
V. I. KONTOS (Β. Ι. ΚΟΝΤΟΣ)
Dept. of Veterinary Public Health, National School of Public Health
M. TAMAMIDOU (Μ. ΤΑΜΑΜΙΔΟΥ)
Dept. of Epidemiology and Biostatistics, National School of Public Health
A. ROUMELIOTOU (Α. ΡΟΥΜΕΛΙΩΤΟΥ)
Dept. of Epidemiology and Biostatistics, National School of Public Health
Abstract

Flow cytometry is an automated, multiparametric and quantitative method that counts and analyses the scattering and fluorescence signals produced as a single cell stream passes through a laser beam. This article describes the method along with fluorochromes used and cell labeling techniques. Additionally, details regarding the applications of flow cytometry are also given. Although flow cytometry is not widely used in veterinary science so far, its application in the near future will rise, since more necessary reagents for veterinary use will be in the market.

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References
Ackley CD., Yamamoto J.K., Levy Ν., Pedersen N.C. and Cooper M.D. (1990). Immunologic abnormalities in pathogen free cats experimentally infected with FIV, J Virology, 64:5652-5655.
Affolter V.K. (2000). Flow cytometry. In: Proceedings of the Workshop on skin immunology organized by ESVD, Saint Paul de Vence, France.
Ashcroft R.G. and Lopez P.A. (2000). Commercial high speed machines open new opportunities in high throughput flow cytometry, J Immunological Methods, 243: 13-24.
Bernander R., Stokke T. and Boye E. (1998). Flow cytometry of bacterial cells: comparison between different flow cytometers and different DNA strains, Cytometry, 31:29-36.
Bourdoiseau G., Bonnefont C, Magnol J.P., Saint-Andre I. and Chabanne L. (1997). Lymphocyte subset abnormalities in canine leshmaniasis, Vet Immunol Immunopathol, 56:345-351.
Byrne K.M., Kim H.W., Chew B.P., Reinhart G.A. and Hayek M.G. (2000). A standardized gating technique for the generation of flow cytometry data for normal canine and normal feline blood lymphocytes, Veterinary Immunology and Immunopathology,
:167-182.
Carter N.P. and Ormerod M.G. (2000). Introduction to the principles of flow cytometry. In: Flow cytometry practical approach, third edition, Oxford University Press, pp:l-22.
Coligan J.E, Kruisbeck AM., Margulies D.H et al. (1995): Current protocols in Immunology. John Wiley & Sons, Inc. Darzynkiewicz Z. and Crissman H.A (1990): Flow cytometry. In: Methods in cell biology, volume 33, San Diego: Academic Press, Inc. USA.
Georgiou M. (2003): Introduction to flow cytometry. Proceedings of Seminar on lymphomas and leukemias, University of Ioannina. Germenis A. (2002): Flow cytometry. Diagnostic Immunology. Academic lectures, University of Thessaly, Larissa.
Hofmann-Lehmann R., Holznagel E., Ossent P. and Lutz H. (1997). Parameters of disease progression in long-term experimental feline retrovirus (FIV and FeLV) infections: hematology, clinical chemistry and lymphocyte subsets, Clin Diagn Lab Immunol, 4:33-42.
Holznagel E., Hofmann-Lehmann R., Allenspach Κ., Huttner S., Willett Β., Groscurth P., Niederer E. and Lutz H. (1996). Flow cytometric detection of activation induced cell death (apoptosis) in peripheral blood lymphocyte subpopulations from healthy cats, Vet Immunol Immunopathol, 52:1-14.
Ibrahim S.F. and van den Engh G. (2003). High speed cell sorting: fundamentals and recent advances, Current Opinion in Biotechnology, 14:5-12.
Lakowicz J.R. (1999). Principles of fluorescence spectroscopy, 2nd edition, New York: Kluwer Academic Plenum Publishers.
Loken M.R., Brosnan J.M., Bach B.A. and Ault K.A. (1990). Establishing optimal lymphocyte gates for immunophenotyping by flow cytometry, Cytometry, 11:453-459.
Marti G.E., Stetler-Stevenson M., Bleesing J.J. and Fleisher T.A. (2001). Introduction to flow cytometry, Seminars in Hematology, volume 38 (2).
Mc Coy J.P. (2002). Basic principles of flow cytometry, Hematol Oncol Clin Ν Am, 16:229-243.
Parks D. R. and Herzenberg L. A (1989): Flow Cytometry and Fluorescence Activated Cell Sorting. In: Paul W. (ed.): Fundamental Immunology, 2nd edition, Raven Press, New York, USA.
Peterakis G. (2003): Flow cytometry. A technology with everyday clinical applications. Proceedings of 29th Hellenic Medical Congress, Athens, pp:33-48.
Riley R.S. (2002). Flow Cytometry and its Applications in Hematology and Oncology, Hematology/Oncology Clinics of North America, vol 16 (2).
Rose N.R., Conway de Macario E., Fahey J.L., Friedman Η. And Renn G.M (1992): Manual of Laboratory Immunology, 4th edition, American Society of Microbiology, Washington DC, USA.
Scheffold A. and Kern F. (2000). Recent developments in flow cytometry, J Clin Immunol, 20: 400-407.
Shapiro H.M (1988): Practical Flow Cytometry, 2nd edition, Alan R. Liss Inc., New York, USA.
Watson J.V. (1991). Introduction to flow cytometry, Cambridge University Press, Cambridge.
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