Isolation, characterization and sequence determination of Noda virus from sea bass (Dicentrarchus labraxL.) reared in freshwater and marine facilities in Greece

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Published: Nov 29, 2017
DOI: https://doi.org/10.12681/jhvms.15074
Keywords:
Dicentrarchus labrax Noda virus isolation characterization viral DNA sequencing
E. XYLOURI (Ε. ΞΥΛΟΥΡΗ)
Department of Anatomy & Physiology of Farm Animals, Faculty of Animal Science, Agricultural University of Athens
J. KOTZAMANIS (Ι. ΚΟΤΖΑΜΑΝΗΣ)
Institut für Physiologische Chemie und Pathobiochemie, Abteilung Angewandte Molekularbiologie Johannes Gutenberg-Universität; Laboratory of Fish Nutrition and Pathology, Institute of Aquaculture, Hellenic Centre for Marine Research
F. ATHANASSOPOULOU (ΑΘΑΝΑΣΟΠΟΥΛΟΥ Φ.)
Laboratory of Ichthyology, Fish Diseases & Aquaculture, Faculty of Veterinary Medicine; School of Health Sciences, University of Thessaly
L. DONG
Department of Anatomy & Physiology of Farm Animals, Faculty of Animal Science, Agricultural University of Athens
A. ARGYROKASTRITIS (ΑΛ. ΑΡΓΥΡΟΚΑΣΤΡΙΤΗΣ)
Department of Anatomy & Physiology of Farm Animals, Faculty of Animal Science, Agricultural University of Athens
E. FRAGKIADAKI (ΕΙΡ. ΦΡΑΓΚΙΑΔΑΚΗ)
Department of Anatomy & Physiology of Farm Animals, Faculty of Animal Science, Agricultural University of Athens
Abstract

The present study aims το characterize Noda virus isolates from freshwater and marine fish, specifically from species Dicentrarchus labrax (sea bass). Previous works reported the detection of Noda virus only by using PCR, in cultured Dicentrarchus labrax showing clinical symptoms. Here we describe the isolation of Noda virus from symptomatic sea bass cultured in sea and freshwater facilities in Greece. The virus was isolated in the continuous cell line SSN -1, exhibiting characteristic cytopathic effect, vacuolation and degeneration of the monolayer. In parallel, in all cerebral tissue samples isolated from infected individuals, a 255 bp viral fragment has been detected, using Reverse Transcription - Polymerase Chain Reaction (RT-PCR) and nested PCR. Comparison of the amplified sequence was detected in diseased fish in European farms and in other piscine species revealed a high nucleotide homology.

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References
Altschul S.F., Gish W., Miller W., Myers E.W. and Lipman D. J., 1990. Basic local alignment search tool. Journal of Molecular Biology 215, 403-410.
Athanassopoulou F., 1990. A study of the Myxosporean infections of R. rutilus L. with special reference to Myxidium rhodei Leger, 1905 in the renal tissue. Ph.D. Thesis, University of Stirling.
Athanassopoulou F., Billinis C, Prapas Th., 2004. Important disease conditions of new cultured species (Acipenser. gueldestaedi, Mugil sp., Dicentrarchus labrax and Silurus sp., Oreochromis sp.) in intensive freshwater farming conditions in Greece: First incidence of Noda virus infection in Acipenser sp. Diseases of Aquatic Organisms, 60, 241-246.
Athanassopoulou F., Billinis C, Psychas V., Karipoglou K., 2003. Viral encephalopathy and retinopathy of Dicentrarchus labrax L. farmed in freshwater in Greece. Journal of Fish Diseases 26,361-365
Bovo G., Nishizawa T., Maltese C, Borghesan F., Multinelli F., Montesi F, De Mas S., 1999. Viral encephalopathy and retinopathy of farmed marine fish species in Italy. Virus Research 631, 43-146.
Breuil G., Mouchel O., Fauvel C, Pepin JF. 2001. Sea bass Dicentrarchus labrax nervous necrosis virus isolates with distinct pathogenicity to sea bass larvae. Diseases of Aquatic Organisms 45 (1),
-31.
Castri J., Thiery R., Jeffroy J., de Kinkelin P., Raymond J.C, 2001. Sea bream Sparus aurata, an asymptomatic contagious fish host for noda virus. Diseases of Aquatic Organisms 47 (1), 33-38.
Comps M., Pepin J.F., Bonami J.R., 1994. Purification and characterization of two fish encephalitis viruses (FEV) infecting Lates calcarifer and Dicentrarchus labrax. Aquaculture, 123, 1-10.
Dalla Valle L., Negrisolo E., Patarnello P., Zanella L., Maltese C, Bovo G., Colombo L. 2001. Sequence comparison and phylogenetic analysis of fish nodaviruses based on the coat protein gene. Archives of Virology 146, 1125-1137.
Dendrinos P., Thorpe P., 1985. Effects of reduced salinity on growth and body composition in the European bass D. labrax. Aquaculture 49, 333-358.
Drury R.A., Wallington E.A. (Eds) 1980. Carleton's histological Techniques, 5th Edition Oxford University Press, Oxford. 520pp.
Fotis G., 1999. Fish Pathology and Aquaculture. Vol. I. Current education editions, Thessaloniki. 382pp.
Frerichs G. N., Tweedie Α., Starkey W. G.,. Richards R. H., 2000. Temperature, pH and electrolyte sensitiviy and heat, Uvand disinfectant inacivation of sea bass D. labrax neuropathy nodavirus. Aquaculture 185,13-24.
Hedge Α., The HC, Lam TJ., Sin YN. (2003). Nodavirus infection in freshwater ornamental fish, guppy, Poicelia reticulatacomparative characterization and pathogenicity studies. Arch Virol. 10, 1007-1019.
Iwamoto T., Mori K., Arimoto M. Nakai T., 1999. High permissivity of the fish cell line SSN-1 for piscine nodaviruses. Diseases of Aquatic Organisms 39 (1), 37-47.
Jalil J., D. Gomez, T. Nakai, I. Sharifpour, C. Shau-Chi, M. Soltani, M. Sharif, Rohani and A. Saidi. (2004): Virology, molecular biology and histopathology investigations on mortality of wild
golden grey mullet (Liza-auratus) in Iranian waters of Caspian Sea. In press.
Kaesbergh P., 1987. In: Rowlands D.J., Mayo M.A., Mahy B.W.J. (Eds.), Molecular Biology of Positive Strand RNA Virus, Academic Press, London, 207-218.
Le Breton et al., 1996 Α., Grisez L., Sweetman J., Ollevier F., 1996. Viral nervous necrosis (VNN) associated with mass mortality in cage reared sea bass (Dicentrarchus labrax). J. Fish Dis. 20,145-Ï5L
Mori K., Nakai T., Muroga K., Arimito M., Mushiake K., Furusawa I., (1992). Properties of a new virus belonging to nodaviridae found in larval striped jack Pseudocaranx dentex with nervous necrosis. Virology 187, 368-371.
Munday BL., Kwang J. & Moody, N., 2002. Betanodavirus infections of teleost fish: a review. Journal of Fish Diseases 25,127-142.
Nagai T., Nishizawa T., 1999. Sequence of the non-structural protein gene encoded by RNA 1 of striped jack nervous necrosis virus. J. Gen. Virol. 80, 3019-302Σ
Nishizawa T., Mori K., Nakai T., Muroga K., 1994. Polymerase chain reaction (PCR) amplification of RNA of striped jack nervous necrosis virus (SJNNV). Diseases of Aquatic Organisms 18,103-107.
RobertsRJ., 1989. Fish Pathology. Bailliere- Tindall. London. 467pp
Roberts R.J., Shepherd C.J., 1997. Handbook of trout and salmon diseases. Fishing News Books, Oxford. 179pp.
Sambrook J., Frisch E.F., Maniatis T., 1989. Molecular cloning. In: A Laboratory Manual, Second Ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
Skliris G.P., Krondiris J.V., Sideris D.C., Shinn A.P., Starkley W.G.. Richards R.H., 2001. Phylogenetic and antigenic characterization of new fish nodavirus isolates from Europe and Asia. Virus Research 75 (1), 59-67.
Thiery R., Raymond J.C, Castri J., 1999a. Natural outbreak of viral encephalopathy and retinopathy in juvenile sea bass Dicentrarchus labrax: study by nested reverse transcriptasepolymerase chain reaction. Virus Research 63, 11-17.
Thiery R., Arnauld C, Delsert C. 1999ß. Two isolates of sea bass D. labrax, nervous necrosis virus with distinct genomes. J. Fish Dis. 22: 201-207.
Ucko M., Colorni Α., Diamant Α., 2004. Nodavirus infections from Israeli mariculture. Journal of Fish Diseases 27 (8), 459- 469.
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