Levels of certain biochemical and oxidative stress parameters in cattle with Brucellosis

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Published: Jan 29, 2018
DOI: https://doi.org/10.12681/jhvms.15470
Keywords:
biochemical parameters Brucella abortus cattle oxidative stress
K. BOZUKLUHAN
Kars Vocational School, Kafkas University
O. MERHAN
Department of Biochemistry, Faculty of Veterinary Medicine, Kafkas University
O. CELEBI
Department of Microbiology, Faculty of Veterinary Medicine, Kafkas University
F. BUYUK
Department of Microbiology, Faculty of Veterinary Medicine, Kafkas University
M. OGUN
Department of Biochemistry, Faculty of Veterinary Medicine, Kafkas University
G. GOKCE
Department of Internal Medicine, Faculty of Veterinary Medicine, Kafkas University
Abstract

The purpose of the present study was to determine concentrations of some biochemical parameters and oxidative stress levels in cattle with brucellosis. For this purpose, a study group included with 20 cattle with brucellosis and a control group with 10 clinically healthy cattle were used. Blood samples were collected into the tubes (with and without anticoagulant agent) from the Jugular vein of animals in each group. The reduced glutathione (GSH) in whole blood and levels of malondialdehyde (MDA) and nitric oxide (NO) were determined spectrophotometrically. Additionally, aspartate aminotransferase (AST), alanine aminotransferase (ALT), urea, creatinine and iron (Fe) levels in serum samples were colorimetrically determined. Compared with the animals in the control group, it was determined that cattle with brucellosis had significantly (P<0.01) higher levels of AST, ALT, creatinine and NO and lower level of Fe. The increases of MDA and GSH levels were moderate and significantly important (P<0.05) while serum urea manner was not significantly altered. It was determined that significant alterations occurred in various biochemical parameters and antioxidant activity decreased in cattle with brucellosis.

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References
Akaike T, Suga M, Maeda H (1998) Free radicals in viral pathogenesis. Molecular mechanisms involving superoxide and NO. Proc Soc Biol Med 217:64-73.
Albayrak A and Albayrak F (2011) Hepatic granulomas associated with brucellosis. Hepat Mon 11:1-2.
Ali AH (2009) The effect of brucellosis on lipid profile and oxidant-antioxidants status. Iraqi J Pharm Sci 18:26-31.
Alton GG, Jones LM, Angus RD, Verger JM (1988) Techniques for the brucellosis laboratory. 190, Institut National de la Recherche Agronomique, Paris, France.
Ariza J, Pigrau C, Canas C, Marron A, Martinez F, Almirante B, Corredoira JM, Casanova A, Fabregat J, Pahissa A (2001) Current understanding and management of chronic hepatosplenic suppurative brucellosis. Clin Infect Dis 32:1024-1033.
Atakisi E, Bozukluhan K, Atakisi O, Gokce HI (2010) Total oxidant and antioxidant capacities and nitric oxide levels in cattle with traumatic reticuloperitonitis. Vet Rec 167:908-909.
Baldwin CL and Winter AJ (1994) Macrophages and Brucella. Immunol Ser 60:363-380.
Beutler E, Duron O, Kelly BM (1963) Improved method for the determination of blood glutathione. J Lab Clin Med 61:882-888.
Cighetti G, Duca L, Bortone L, Sala S, Nava I, Fiorelli G, Cappellini MD (2002) Oxidative status and malondialdehyde in β thalassaemia patients. Eur J Clin Invest 32:55-61.
De Groote MA and Fang FC (1995) NO inhibitions: Antimicrobial properties of nitric oxide. Clin Infect Dis 21:162-165.
Değer S, Değer Y, Ertekin A, Gül A, Biçek K, Özdal N (2008) Determination of the status of lipid peroxidation and antioxidants in cattle infected with Dictyocaulus viviparus. Turkiye Parazitol Derg 32:234-237.
El-Boshy M, Abbas H, El-Khodery S, Osman S (2009) Cytokine response and clinicopathological findings in Brucella infected camels (Camelus dromedarius). Veterinarni Medicina, 54:25-32.
Elazab MFA (2015) Evalution of serum enzyme activities and protein fractions in Brucella- infected cows. Turk J Vet Anim Sci 39:480-484.
Gokce HI and Woldehiwet Z (1999) The effects of Ehrlichia (Cytoecetes) phagochytophila on the clinical chemistry of sheep and goats. J Vet Med 46:93-103.
Gruys E, Obwolo MJ, Toussaint MJM (1994) Diagnostic significance of the major acute phase proteins in veterinary clinical chemistry. A review. Vet Bull 64:1009-1018.
Halliwell B (1994) Free radicals, antioxidants, and human disease: curiosity, cause or consequence? Lancet 344:721-724.
İbrahim AIA, Awad R, Shetiy SD, Saad M, Bilal NE (1988) Genito-urinary complications of brucellosis. Br J Urol 61:294-298.
Jiang X, Leonard B, Benson R, Baldwin CL (1993) Macrophage control of Brucella abortus: Role of reactive oxygen intermediates and nitric oxide. Cell Immunol 151:309-319.
Kaneko JJ, Harvey JW, Bruss ML (2008) Clinical Biochemistry of Domestic Animals. 6th Ed., Academic Press, New York: pp 364-390.
Kataria N, Kataria AK, Maan R, Gahlot AK (2010) Evalution of oxidative stress in brucella infected cows. J Stress Physiol&Biochem 6:19-25.
Kreil TR and Eibl MM (1996) Nitric oxide and viral infection: NO antiviral activity against a flavivirus in vitro, and evidence for contribution to pathogenesis in experimental infection in vivo. Virology 219:304-306.
Kushwaha N, Rajora VS, Mohan A, Singh JL, Shukla SK (2014) Assessment of haemato-biochemical parameters and therapeutics on Brucella infected cattle. J Microbiol Exp 1:1-5.
Madebo T, Lindtjorn B, Aukrust P, Berge RK (2003) Circulating antioxidants and lipid peroxidation products in untreated tuberculosis patients in Ethiopia. Am J Clin Nutr 78:117-122.
Marletta MA (1988) Mammalian synthesis of nitrite, nitrate, nitric oxide, and N-nitrosating agents. Chem Res Toxicol 1:249-257.
Miranda KM, Espey MG, Wink DA (2001) A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide Biol Ch 5:62-71.
Neta AVC, Mol JPS, Xavier MN, Paixao TA, Lage AP, Santos RL (2010) Pathogenesis of bovine brucellosis. Vet J 184:146-155.
Nicoletti P (2010) Brusellosis: past, present and future. Prilozi 31:21-32.
Nisbet C, Yarım GF, Çiftci A, Çenesiz S, Çiftci G (2007) Investigation of serum nitric oxide and malondialdehyde levels in cattle infected with Brucella abortus. Vet J Ankara Univ 54:159-163.
Otlu S, Sahin M, Atabay HI, Unver A (2008) Serological investigations of brucellosis in cattle, farmers and veterinarians in the Kars district of Turkey. Acta Vet Brno 77:117-121.
Öztürk H and Büyük F (2015) Comparison of the methods used in serological diagnosis of Brucellosis in farm animals. Atatürk Üniversitesi Vet Bil Derg 10:6-12.
Pekmezci D, Çenesiz S, Çakıroğlu D, Çiftci G, Çıra A, Gökalp G (2012) Status of lipid peroxidation, cell destruction and the antioxidant capacity in foals with lower respiratory tract disease. Kafkas Univ Vet Fak Derg 18:157-160.
Petermann H, Vogl S, Schulze E, Dargel R (1999) Chronic liver injury alters basal and stimulated nitric oxide production and 3H-thymidine incorporation in cultured sinusoidal endothelial liver cells from rats. J Hepatol 31:284-292.
Serefhanoglu K, Taskin A, Turan H, Timurkaynak FE, Arslan H, Erel O (2009) Evalution of oxidative status in patients with brucellosis. Braz J Infect Dis 13:249-251.
Surapaneni KM and Venkataramana G (2007) Status of lipid peroxidation, glutathione, ascorbic acid, vitamin E and antioxidant enzymes in patients with osteoarthritis. Indian J Med Sci 61:9-14.
Taylor-Robinson AW, Severn A, Phillips RS (1996) Kinetics of nitric oxide production during infection and reinfection of mice with Plasmodium chabaudi. Parasite Immunol 18:425-430.
Yoshioka T, Kawada K, Shimada T, Mori M (1979) Lipid peroxidation in maternal and cord blood and protective mechanism against activatedoxygen toxicity in the blood. Am J Obstet Gynecol 135:372-376
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