The prevalence estimates of Mycobacterium bovis infection in cattle with ELISA

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DOI: https://doi.org/10.12681/jhvms.16050
Keywords:
tuberculosis cattle prevalence estimates ELISA
F. BUYUK
Department of Microbiology, Faculty of Veterinary Medicine, Kafkas University
K. BOZUKLUHAN
Kars Vocational School, Kafkas University
A. GULMEZ SAGLAM
Department of Microbiology, Faculty of Veterinary Medicine, Kafkas University
G. GOKCE
Department of Internal Medicine, Faculty of Veterinary Medicine, Kafkas University
O. CELEBI
Department of Microbiology, Faculty of Veterinary Medicine, Kafkas University
E. CELIK
Department of Microbiology, Faculty of Veterinary Medicine, Kafkas University
S. KIZILTEPE
Department of Internal Medicine, Faculty of Veterinary Medicine, Kafkas University
M. R. COSKUN
Department of Microbiology, Faculty of Veterinary Medicine, Kafkas University
S. OTLU
Department of Internal Medicine, Faculty of Veterinary Medicine, Kafkas University
M. SAHIN
Department of Microbiology, Faculty of Veterinary Medicine, Kafkas University
Abstract

Tuberculosis is an infectious bacterial disease of Bovidae and the causative agent is Mycobacterium bovis. It is responsible for remarkable economic losses among cattle herds with widely dispersion. Prompt and consistent diagnosis of tuberculosis especially in countries where the disease is endemic as in Turkey is of great importance to detect and identify infectious cases for strengthening control measures. In the present study, it was aimed to detect true animal and herd prevalence (within-herd, and between-herd) of antibodies against M. bovis in cattle herds. A serologic survey for antibody detection against the M. bovis was conducted by using an ELISA kit. Thirty three cattle herds were randomly selected from different farms and totally 460 cattle over five years of age were sampled. The true animal, within-herd, and between-herd prevalences found were 5.9% (95% CI = 3.0 to 8.8), 11.1% (95 CI = 6.5 to 15.8) and 73.4% (95 CI = 51.2 to 95.6), respectively. Results will provide useful information about the status of M. bovis infection and will contribute to the disease control practices.

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References
Adams LG (2001) In vivo and in vitro diagnosis of Mycobacterium bovis infection. Rev Sci Tech OIE, 20, 304-324, 2001.
Alito A, Mcnair J, Girvin RM, Zumarraga M, Bigi F, Pollock JM, Cataldi A (2003) Identification of Mycobacterium bovis antigens by analysis of bovine T-cell responses after infection with a virulent strain. Braz J Med Biol Res 36:1523-1531.
Beytut E (2001) Pathological examination on the localization of lesions and incidence of tuberculosis in cattle in Kars City and its surrounding. Kafkas Univ Vet Fak Derg 7: 15-25.
Buyuk F, Celebi O, Akça D, Otlu S, Tazegul E, Gulmez A, Sahin M (2014) Estimated apparent and true prevalences of paratuberculosis in sheep herds of the Kars Region in Northeastern Turkey. Vet Med-Czech 59:331-335.
Lilenbaum L and Fonseca LS (2006) The use of ELISA as a complementary tool for bovine tuberculosis control in Brazil. Braz J Vet Res Anim Sci 43:256-261.
Monaghan ML, Doherty ML, Collins JD, Kazda JF, Quinn PJ (1994) The tuberculin test. Vet Microbiol 40:111-124.
OIE (2004) Bovine tuberculosis. In: Manual of diagnostic tests and vaccines for terrestrial animals, 5th ed. Office International des Epizooties, Paris (updated 23 July 2004), Chapter 2.3.3. 2004.
OIE (2011) World Animal Health Information Database. http://www.oie.int/wahis_2/public/wahid.php/Diseaseinformation/statusdetail.2011.
OIE (2012) Procedure for registration of diagnostic kits abstract sheet IDEXX M. bovis antibody test kit, IDEXX Laboratories, www.oie.int.Accessed August/11/2012.
Ozturk D, Pehlivanoglu F, Kale M, Tok AA, Guldali Y, Turutoglu H (2010) In vitro diagnosis of bovine tuberculosis by γ-interferon assay. Kafkas Univ Vet Fak Derg 16:875-878.
Phillips CJ, Foster CR, Morris PA, Teverson R (2003) The transmission of Mycobacterium bovis infection to cattle. Res Vet Sci 74:115.
Rogan WJ and Gladen B (1978) Estimating prevalence from the results of a screening test. Am J Epidemiol 107: 71-76.
Sayin Z and Erganis O (2013) Diagnosis of bovine tuberculosis by PPD-ELISA and sonication-ELISA. Isr J Vet Med 68:180-184.
Schiller I, Oesch B, Vordermeier HM, Palmer MV, Harris BN, Orloski KA, Buddle BM, Thacker TC, Lyashchenko KP, Waters WR (2010)
Bovine tuberculosis: A review of current and emerging diagnostic techniques in view of their relevance for disease control and eradication. Transbound Emerg Dis 57:205-220.
Souza II, Melo ES, Ramos CA, Farias TA, Osoria AL, Jorge KS, Vidal CE, Silva AS, Silva MR, Pellegrin AO, Araujo FR (2012)
Screening of recombinant proteins as antigens in indirect ELISA for diagnosis of bovine tuberculosis. SpringerPlus 1:2-6.
Speybroeck N, Devleesschauwer B, Joseph L, Berkvens D (2013) Misclassification errors in prevalence estimation: Bayesian handling with care. Int J Public Health 58:791-795.
Unver A, Atabay HI, Gunes V, Citil M, Erdogan HM (2007) The prevalence of bovine tuberculosis in Kars District analyzed by PCR. Kafkas Univ Vet Fak Derg 13:27-31.
Wadhwa A, Hickling GJ, Eda S (2012) Opportunities for improved serodiagnosis of human tuberculosis, bovine tuberculosis, and paratuberculosis. Vet Med Int 2012: 674238.
Wadhwa A, Johonson RE, Eda K, Waters WR, Palmer MV, Bannantine JP, Eda S (2014) Evaluation of ethanol vortex ELISA for detection of bovine tuberculosis in cattle and deer. BMC Vet Res 10:2-6.
Welsh MD, Cunningham RT, Corbett D, Girvin RM, Mcnair J, Skuce RA, Bryson DG, Pollock JM (2005) Influence of pathological progression on the balance between cellular and humoral immune responses in bovine tuberculosis. Immunology 114:101-111.
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