Comparison of two ELISA methods for detection of antibodies against Footand- Mouth Disease virus of cattle breeds in Turkey
The study was conducted using two ELISA methods - the liquid phase blocking ELISA (LPBE) and solid phase competition ELISA (SPCE) for the detection of foot-and-mouth disease virus (FMDV) serotype A- and O-specific antibodies of different cattle breeds in Turkey. These methods were compared in 426 cattle previously vaccinated with oil-adjuvanted bivalent vaccine as well as in sera from 40 cattle with no history of foot-and-mouth disease infection or vaccination. The results were found that SPCE had a better specificity (serotype A; 100% and serotype O; 97.50%) than LPBE (serotype A 95.00% and serotype O 92.50%). Sensitivity of SPCE had also better values (serotype A; 99.30% and serotype O; 98.59%) than LPBE (serotype A; 97.89% and serotype O; 96.48%). The results of the present study showed that the SPCE method is more reliable than LPBE.
- How to Cite
INCE, Ö. B., KALKAN, R., & ÇAKIR, S. (2018). Comparison of two ELISA methods for detection of antibodies against Footand- Mouth Disease virus of cattle breeds in Turkey. Journal of the Hellenic Veterinary Medical Society, 67(1), 33–38. https://doi.org/10.12681/jhvms.15621
- Vol. 67 No. 1 (2016)
- Research Articles
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