Comparison of two ELISA methods for detection of antibodies against Footand- Mouth Disease virus of cattle breeds in Turkey


Published: Jan 29, 2018
Keywords:
Foot-and-mouth disease liquid-phase blocking ELISA solid-phase competition ELISA
Ö. B. INCE
R. KALKAN
S. ÇAKIR
Abstract

The study was conducted using two ELISA methods - the liquid phase blocking ELISA (LPBE) and solid phase competition ELISA (SPCE) for the detection of foot-and-mouth disease virus (FMDV) serotype A- and O-specific antibodies of different cattle breeds in Turkey. These methods were compared in 426 cattle previously vaccinated with oil-adjuvanted bivalent vaccine as well as in sera from 40 cattle with no history of foot-and-mouth disease infection or vaccination. The results were found that SPCE had a better specificity (serotype A; 100% and serotype O; 97.50%) than LPBE (serotype A 95.00% and serotype O 92.50%). Sensitivity of SPCE had also better values (serotype A; 99.30% and serotype O; 98.59%) than LPBE (serotype A; 97.89% and serotype O; 96.48%). The results of the present study showed that the SPCE method is more reliable than LPBE.

Article Details
  • Section
  • Research Articles
Downloads
Download data is not yet available.
References
Batista A, Quattrocchi V, Olivera V (2010) Adjuvant effect of Cliptox on the protective immune response induced by an inactivated vaccine against foot and mouth disease virus in mice. Vaccine, 28:6361-6
Brocchi E, Grazioli S, Yadin H, Simone F (2004) Validation of a solid phase competitive ELISA (SPBE) based on the use a single neutralizing monoclonal antibody for the measurement of antibodies to FMDV type Asia 1. In: Session of the Research Group Standing Technical Committee of the European Commission for the Control of Foot and Mouth Disease. Crete, Greece: EuFMD, pp: 288–297.
Bulut N and Aktas S (2006) Turkey report to the EUFMD Commission, FMD in Turkey. In: Report of the 73rd Session of the EUFMD Executive Committee. Rome: FAO, pp: 23–27.
Chenard G, Miedema K, Moonen P, Schrijver RS, Dekker A (2003) A solid-phase blocking ELISA for detection of type O foot-andmouth disease virus antibodies suitable for mass serology. J. Virol. Methods, 197:89-98.
Clavijo A, Wright P, Kitching P (2004) Developments in diagnostic techniques for differentiating infection from vaccination in foot and mouth disease. Vet J, 167: 9–22.
Doel TR (2003) FMD vaccines. Virus Research 91(1):81–99.
FMD-Disconvac. (2013) Assessment and improvement of heterologous protection by FMD vaccines. Work package 3. Veterinary and Agrochemical Research Centre, Brussels, Belgium, pp:3-5
Golding SM, Hedger RS and Talbot P (1976) Research in Veterinary Science 20: 142-147.
Haas B (1994) Application of the FMD liquid-phase blocking sandwich ELISA. Problems encountered in import/export serology and possible solutions. In: Session of the Research Group of the European Commission for the Control of Foot and Mouth Disease. Vienna, Austria: EuFMD pp: 124–127.
Hamblin C, Barnett ITR, Hedger RS (1986) A new enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against foot-and-mouth disease virus. I. Development and method ELISA. J Immunol Methods 93: 115–121.
Habiela M, Ferris NP, Hutchings GH, Wadsworth J, Reid SM, Madi M, Ebert K, Sumption KJ, Knowles NJ, King DP, Paton DJ (2010) Molecular characterization of foot-and-mouth disease viruses collected from Sudan. Transbound. Emerg. Dis. 57:305–314
Jacobson RH (1998) Validation of serological assays for diagnosis of infectious diseases. Rev Sci Tech Off Int Epiz, 17: 469–486.
Jaworski JP, Compaired D, Trotta M, Perez M, Trono K, Fondevila N (2011) Validation of an r3AB1-FMDV-NSP ELISA to distinguish between cattle infected and vaccinated with foot-and-mouth disease virus. J Virol Methods,178: 191-200.
Klein J, Parlak U, Ozyoruk F, Christensen LS (2006) The molecular epidemiology of foot-and-mouth disease virus serotypes A and O from 1998 to 2004 in Turkey. BMC Vet Res, 4: 2–35.
Knight-Jones TJD, Rushton J (2013) The economic impacts of foot and mouth disease– what are they, how big are they and where do they occur? Prev Vet Med; 112(3–4):161–73.
Li Y, Swabey KG, Gibson D, Keel PJ, Hamblin P, Wilsden G, Corteyn M, Ferris NP (2012) Evaluation of the solid phase competition ELISA for detecting antibodies against the six foot-and-mouth disease virus non-O serotypes. Journal of Virological Methods 183 (2), pp. 125-131.
Li-na M, Jie Z, Hao-tai C, Jian-hua Z, Yao-zhong D and Yong-sheng L (2011) An overview on ELISA techniques for FMD. Virology Journal 8:419
Mackay DKJ, Rendle T and Armstrong RM (1994) Report of the Session of the Research Group of the Standing Technical Committee of the European Commission for the Control of Footand-Mouth Disease, Vienna, 19-22 September, 1994,pp: 128-145.
Mackay DKJ, Rendle T and Kitching RP (1998) Report of the Session of the Research Group of the Standing Technical Committee of the European Commission for the Control of Foot-and-Mouth Disease, Aldershot, UK, 14-18 September, 1998, pp:148-157.
Mackay DKJ, Bulut AN, Rendle T, Davidson F, Ferris NP (2001) A solid-phase competition ELISA for measuring antibody to foot and- mouth disease virus. J Virol Methods, 97: 33–48.
Martinez CS and Quintero M (1997). The use of a liquid phase blocking ELISA kit for detection of antibodies against foot-and-mouth disease virus in Colombia. In: Proceedings of the Use of ELISA for Epidemiology and Control of Foot-and-Mouth Disease and Bovine Brucellosis in Latin America. Vienna, Austria, pp:13-17
McKercher PD, Graves JH (1977) A review of the current status of oil adjuvants in foot-and-mouth disease vaccines. Dev Biol Standard 35: 107–112.
Neeta L, Rajib D, Ak S, Tilling T, Awachat V and Saxena V (2011) A Brief Review on Diagnosis of Foot-and-Mouth Disease of Livestock: Conventional to Molecular Tools Veterinary Medicine International Volume 2011, Article ID 905768, doi:10.4061/2011/905768
Niedbalski W, Fitzner A, Paprocka G, Kęsy A (1994) Application of liquid-phase blocking sandwich ELISA (LPBE) for the detection of antibodies against foot-and mouth disease virus. Bull. Vet. Inst. Pulawy, 38: 105-114.
OIE (2000) Manual of Standards for Diagnostic Tests and Vaccines. 4th ed. Paris, France: OIE, pp:1-8
OIE (2012) Chapter 2.1.5. Foot and mouth disease. In Manual of diagnostic tests and vaccines for terrestrial animals. OIE, Paris, France. htt p://w w w.oie.int/f ileadmin / Home/eng/ Health_standards/tahm/2.01.05_FMD.pdf.
OIE (2012) Manual of Diagnostic Tests and Vaccines for Terrestrial Animals.6th ed. Paris, France:OIE, pp:1-12
Paiba GA, Anderson J, Paton DJ, Soldan AW, Alexandersen S, Corteyn M, Wilsden G, Hamblin P, MacKay DK, Donaldson AI (2004) Validation of a foot-and-mouth disease antibody screening solid- phase competition ELISA Journal of Virological Methods 115: 145–158.
R Core Team (2014). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. Available online: http://www.R-project.org, 2014
Şevik M and Öztürk FF (2013) Comparative evaluation of liquid- phase blocking ELISA and solid-phase competition ELISA methods for the detection of antibodies to the structural proteins of foot-and mouth disease types O and A viruses. Turk J Vet Anim Sci, 37:523-528
Valdazo-Gonzalez B, Polihronova L, Alexandrov T, Normann P, Knowles NJ, Hammond JM (2012) Reconstruction of the transmission history of RNA virus outbreaks using full genome sequences: foot-and-mouth disease virus in Bulgaria in 2011. PLoS ONE, 7(11):e49650
Van Bekkum JG, Frenkel HS, Frederiks H HJ a nd Frenkel S (1959b) Observations on the carrier state of cattle exposed to foot-and-mouth disease virus. Tijdschriet voor Diergeneeskunde, 84:1159–1164.
Most read articles by the same author(s)