Comparison of two ELISA methods for detection of antibodies against Footand- Mouth Disease virus of cattle breeds in Turkey

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Publicado: Jan 29, 2018
DOI: https://doi.org/10.12681/jhvms.15621
Ö. B. INCE
Agriculture and Rural Development Support Institution
R. KALKAN
Institute for Foot and Mouth Disease
S. ÇAKIR
University of Abant İzzet Baysal, Department of Poultry Science, Faculty of Agriculture and Natural Sciences
Resumen

The study was conducted using two ELISA methods - the liquid phase blocking ELISA (LPBE) and solid phase competition ELISA (SPCE) for the detection of foot-and-mouth disease virus (FMDV) serotype A- and O-specific antibodies of different cattle breeds in Turkey. These methods were compared in 426 cattle previously vaccinated with oil-adjuvanted bivalent vaccine as well as in sera from 40 cattle with no history of foot-and-mouth disease infection or vaccination. The results were found that SPCE had a better specificity (serotype A; 100% and serotype O; 97.50%) than LPBE (serotype A 95.00% and serotype O 92.50%). Sensitivity of SPCE had also better values (serotype A; 99.30% and serotype O; 98.59%) than LPBE (serotype A; 97.89% and serotype O; 96.48%). The results of the present study showed that the SPCE method is more reliable than LPBE.

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