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In vivo ultrasound guided transvaginal oocyte collection in the cow

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G. S. AMIRIDIS (Γ.Σ. ΑΜΟΙΡΙΔΗΣ), M. SALAHEDDINE, I. A. JEFFCOATE, E. VAINAS (Ε. ΒΑΪΝΑΣ), L. ROBERTSON
G. S. AMIRIDIS (Γ.Σ. ΑΜΟΙΡΙΔΗΣ), M. SALAHEDDINE, I. A. JEFFCOATE, E. VAINAS (Ε. ΒΑΪΝΑΣ), L. ROBERTSON

Abstract


This paper describes the results of the in vivo ultrasound guided follicular aspiration for ovum pick υρ (OPU) in the cow. Twelve non pregnant dry cows aged 4-6 years were used in this experiment. Eight cows underwent OPU during three successive oestrous cycles and another four cows were used as controls having only transvaginal ultrasound scanning of their ovaries. Oocyte collection took place three times during the luteal phase of each natural oestrous cycle (days 3-4,9-11 and 14-17). The content of 326 follicles with a diameter of 4-15mm was aspirated and 104 oocytes were collected (recovery rate 31.9% or 1.55 oocytes per cow and session). The oocyte recovery rate increased after the first three sessions (from 13.04% to 35.0%) and reached levels of υρ to 52.6%. More follicles were aspirated on days 9-11 (133 follicles 40.8%) compared to 111 (34%) follicles on days 14-17 and 82 (25%) on days 3-4) (P<0.05). The evaluation of the collected oocytes revealed that 60 oocytes (57.7%) were suitable for further in vitro manipulation. Neither the origin of the oocyte (left or right ovary) nor the stage of the oestrous cycle affected the recovery rate or the quality of the collected oocytes. There was no significant difference either in the length of the oestrous cycle between the experimental animals and the controls (21.6± 1.4 vs. 22.37±1.0 respectively), or in plasma progesterone concentration in daily collected blood samples from the animals of the two groups. The results of this study are compared to those from the international literature and to the results from endoscopical methods for oocyte recovery. The feasibility of application of this technique to projects designed to improve the genetic merit of cows is discussed.


Keywords


συλλογή ωαρίων; υπερηχογραφία; αγελάδα

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References


Gordon I. Laboratory production of cattle embryos. CAB International, Wallingford, Oxon 1994.

Lambert RD. Bernard C, Rioux JE, Eeland R, D'Amours D and Montreuil A. Endoscopy in cattle by paralumbar route: technique for ovarian examination and follicular aspiration. Theriogenology 1983, 20, 149-161.

Reichenbach HD. Wicbke NH, Besenfelder UH, Modi J and Brem G. Transvaginal laparoscopy-guided aspiration of bovine follicular oocytes: preliminary results. Theriogenology 1993,39,295.

Dellenbach Ρ, Nisand I, Morreau L, Feger B, Plumere C and Gerlinger P. Transvaginal sonographically controlled follicle puncture for oocyte retrieval. Fertility and Sterility 1985, 44, 656-662.

Callesen H, Greve Τ and Cristensen F. Ultrasonically guided aspiration of bovine follicular oocytes. Theriogenology 1987 27.217

Pieterse M.C, Kappen K.A, Kruip ΤΑΜ and Taverrie MAM. Aspiration of bovine oocytes during transvaginal ultrasound scanning of the ovaries. Theriogenology 1988,30, 751-762.

van der Schams A, van der Westeriaken LAJ, de Wit AAC, Eystone WE and de Boer HA. Ultrasound guided transvaginal collection of oosytes in the cow. Theriogenology

,288.

S. Pieterse MC Vos PLAM, Kruip ΤΑΜ, Wurth YA. Tranvaginal ultrasound guided follicular aspiration of bovine. Theriogenology 1991; 35, 857-862.

Scott CA, Robertson L, de Moura RTD, Paterson C. Boyd JS. Technical aspects of transvaginal ultrasound-guided follicular aspiration in cows. Veterinary Record 1994, 134,440-443.

Bungartz L, Lucas-Hahn A, Rath D Niemann. Collection of oocytes from cattle via follicular aspiration aided by ultrasound with or without gonadotropin pretreatment and in different reproductive stages. Theriogenology 1995, 43, 667-675.

Rajakoski E. The ovarian fiollicular system in sexually mature heifers with special reference to seasonal, cyclical and leftright variations. Acta Endocrinologica 1960, 52, 7-68.

Ireland JJ and Roche JF. Development of nonovulatory antral follicles in heifers: changes in steroids in follicular fluid and receptors for gonadotropins. Endocrinology 1983. 112, 150-156.

Fortune JE, Sirois J, Quirk SM. The growth and differentiation of ovarian follicles during the bovine oestrus cycle Theriogenology 1988, 29, 95-109.

Ireland JJ and Roche JF. Hypothesis regarding development of dominant follicles during a bovine estrus cycle. In: Follicular growth and ovulation rate in farm animals.

J.F.Roche, D. O'Challagan (eds) MartinusNijhoff Publishers Dordrecht 1987, 1-18.

Fortune JE. Sirois J, Turzillo AM, Lavour. M. Follicle selection in domestic ruminants. Journal of Reproduction and Fertility suppl 1991,43. 187-198.

Duffour JJ, Roy GL. Distribution of ovarian follicular population in the dairy cow within 35 days after postparturition. Journal of Reproduction arid Fertility 1985, 73:229-235.

Gibbons JR. Beai WE, Krisher RL. Faber EG. Pearson RE and Gwazdauskas FC. Efifects of once versus twice-weekly transvaginal follicular aspiration on bovine oocyte recovery and embryo development. Theriogenology 1994,42,405-419.

Kruip ΤΑΜ. Boni R, Wurth YA, Roelofsen MWM. And Pieterse MC. Potential use of ovum pick-up for embryo production and, breeding in cattle Theriogenology 1994, 42 675-684.

Duby RT, Damiani Ρ, Looney CR, Fissare RA and Robl JM. Prebuberal calves as oocyte donors: Promises and Problems. Theriogenology 1996.45 121-130.

Ryan DB, Blakewood EG, Swanson WS, Rodriguez Η and Godke RA. Using hormone treated pregnant cows as a potential source of oocytes for iri vitro fertilization. Theriogenology 1993,40: 1039-1055.

Looney CR, Lindsey BR. Gonseth CL, Johnson DL. Commercial aspects of oocytes retrieval and in vitro fertilization (IVF) for embryo production in problem cows. Theriogenology 1994,41 ,67-72.

Corrie JET, Hunter WM, Macpherson JS. A strategy for radioimmunoassay of plasma progesterone with use of homologous-site 1251-labelled radioligand. Clinical Chemistry 1981,27,594-599

Jeffcoate IA. Concentrations of luteinizing hormone and oestradiol in plasma, and response to injection of gonadotrophin-releasing hormone analogue at selected stages of anoestrus in domestic bitches. Journal of Reproduction and Fertility 1992, 94, 423-429.

de Loos F, Van Viet C, Van Mauric Ρ and Kruip Th AM. Morphology if immature bovine oocytes. Gamete Research 1989,24,197-204.

Stubbings RB and Walton JS. Effect of ultrasonically-guided follicle aspiration on estrus cycle and follicu(ar dynamics in Holstein cows. Theriogenology 1995,43, 705-712.

Bergfelt DR. Lighlfoot KC. and Adams GP. Ovarian dynamics following ultrasound-guided transvaginal follicle ablation in cyclic heifers. Theriogenology 1994,41 , 161 .

Adams GP, Matteri RL, Kastelic JP, Ko JCH. and Ginther OJ. Association between surges of follicle stimulating hormone and emergence of follicular waves in heifers. Journal of Reproduction and Fertility 1992. 94, 177-188.

Am iridis GS. Effects of follicular aspiration on the bovine oestrus cycle. Ph.D. Thesis Glasgow University Veterinary School, 1996.

Glencross RG, Bleach EC, McLeod BJ, Beard AJ and Knight PG. Increased ovulation rate in heifers immunized against a synthetic peptide sequence of bovine inhibin. Journal of Reproduction and Fertility 20, 1990 abstract series No 5.

Βαϊν&ς Ε, Βαφειάδης Δ, Μττόακος Κ, Τσακάλχοφ Π. In vitro ωρίμανση και γονιμοποίηση ωαρίων αγελάδας και καλλιέγεια του ξυγωτού. Δελτίο Ελληνικής Κτηνιατρικής Εταιρίας 1994,45,31-36.

Τοακάλωφ Π.Μαιευτική των κατοικίδιων ζώων. Θεσσαλονίκη, 1983

Vos PLAM, de Loos FAM, Pieterse MC, Bevers MM, Taverne MAM. and Dieleman. SJ. Evaluation of transvaginal ultrasound guided follicle puncture , to collect oocytes and follicular fluids at consecutive times relative to the preovulatory LH surge in eCG/PG-treated cows. Theriogenology, 1994,4L 829-840.

Kruip ΤΑΜ, Pieterce MC, van Beneden TH, Vos PLAM, Wurth YA and Taverne MAM. Increased success rate of IVM and IVF in the bovine after sonographic guided

transvaginal collections of the oocytes. Theriogenology 1990, 33,269

Chian RC. Niwa Κ and Sirard MA. Effects of cumulus cells on male pronuclear formation and subsequent early development of bovine oocytes in vitro Theriogenology 1994, 41,1499-1508.

Matton P, Acelakoun V, Couture Y and Dufour JJ. Growth and replacement of the bovine ovarian follicles during the estrus cycle. Journal of Animal Science 1981 ,52,813-820.

Wiebke N. Ex vivo gewinnung boviner Cumulus-Oozyten Komplexe durch transvaginale, laparoskopisch geführte follikepuntion. Ph.D. Thesis. München 1993.

Wenigerkind H. Untersuchungen zur ex vivo gewinnung boviner CumulusOozyten Komplexe mittels endoskopish geführte follikepuntion. Ph.D. Thesis. München 1995.

Arthur GH, Noakes DE, Pearson H. (eds). Veterinary reproduction and obstetrics, Bailliere Tindail, London 1989.


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