Laparoscopie collection and transfer of Chios sheep embryos

PDF
Published: Jan 31, 2018
DOI: https://doi.org/10.12681/jhvms.15717
G. S. AMIRIDIS (Γ.Σ. ΑΜΟΙΡΙΔΗΣ)
Dept. Of Obstetrics and Reproduction, Faculty of Veterinary Medicine University of Thessaly
B. KUEHHOLZER
LBI fuer zyto-, immuno-und molekulargenetische Forschung, Wien
U. BESENFELDER
Institute of Animal Breeding and Genetics, University of Animal Science, Vienna, Austria
A. LYMBEROPOULOS (Α. ΛΥΜΠΕΡΟΠΟΥΛΟΣ)
N.AG.RE.F, Veterinary Research Institute, Thessaloniki
E. VAINAS (Ε. ΒΑΪΝΑΣ)
N.AG.RE.F, Veterinary Research Institute, Thessaloniki
Abstract

The efficiency of two different laparoscopic techniques for embryo collection was examined. Two groups (A and B) of 12 Chios breed ewes were used as donors and 20 crossbred Chios ewes as recipients. Oestrus was synchronised by intravaginal sponges, the donors were supervoulated by 8,8 mg of ovine FSH and laparoscopic intrauterine artificial insemination was conducted with extended fresh ram semen. Embryo collection was carried out the 6th day after AI. The uteri of the animals of group A were flushed after catheterisation of the ipsilateral oviduct. In the animals of group Β a flushing catheter was inserted close to the tip of the horn. Fifty nine and 46 embryos were collected from groups A and Β corresponding to recovery rate of 51.75% and 39% respectively (P>0.05). Laparoscopic transfer of 43 fresh embryos resulted to 11 (55%) pregnancies with (39.5%) surviving fetuses.

Article Details
  • Section
  • Research Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Authors who publish with this journal agree to the following terms:

· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Downloads
Download data is not yet available.
References
Hunter GL, Adams CE and Rowson LE. Inter - Breed ovum transfer in sheep. J Agric Sci 1955,46:143-145.
Cutten IN. Immediate and long term effects of post mating on the lambing performance of the ewes. Proc Aust Soc Anim Prod 1970,8:338-393.
Coonran SA, Coren BR, McBride BL, Bowen ML, Kraemer DC. Successful non-surgical collection of ovine embryos. Theriogenology 1986,25,149.
Coondod SA, Bowen J and Kraemer DC. Non surgical collection of ovine embryos. Proc of the 5th Ann Con of the Amer Embryo Transfer Assoc 1984,83-87.
McKelvey WAC, Robinson J J, Aitken RP. A simplified technique for the transfer of ovine embryos by laparoscopy. Vet Ree 1985,117:492-494.
McKelvey WAC, Robinson JJ, Aitken RP, Robertson IS. Repeated recoveries of embryos from ewes by laparoscopy. Theriogenology 1986,25,855-865.
Nellenschulte E, Niemann H. Collection and transfer of ovine embryos by laparoscopy. Anim Reprod Sci 1992,27,293-304.
Scudamore LL, Robinson JJ, Aitken RP, Kennedy DJ, Ireland 5 and Robertson JS. Laparoscopy for intrauterine insemination and embryo recovery in superovulated ewes at a commercial embryo transfer unit. Theriogenology 1991,35,329-337.
Coonrod SA, Coren BR, McBride BL, Bowen MJ, Kraemer DC, Successfull non-surgical collection of ovine ova. Therigenology 1986,25,149.
Mylne MJA, McKelvey WAC, Fernie k, Matthews K. Use of a transcervical technique for embryo recovery in sheep. Vet Ree 1992,130,450-451.
Tervit HR and Havik PG. A modified technique for flushing ova from the sheep uterus. N.Z.Vet Jour. 1976,24,138-140.
Σαμαρτζή Φ. Μελέτη της ανταπόκρισης των προβάτων φυλής Χίου στην πρόκληση πολλαπλής ωοθυλακιορρηξίας μετά από χορήγηση PMSG και in vitro εκτίμηση της βιωσιμότητας των εμβρύων. Διδακτορική Διατριβή, ΑΠΘ 1993.
Samartzi F, Belibasaki S, Vainas E, Boscos C. Plasma progesterone concentration in relation to ovulation rate and embryo yield in Chios ewes superovulated with PMSG. Anim Repro Sci 1995, 39,11-21.
Samartzi F, Boscos C, Vainas E, Tsakalof P. Superovulatory response of Chios sheep to PMSG during spring and autumn. Anim Repro Sci 1995,39,215-222.
Hafez ESE. Reproduction in farm animals. 6th edition Lea 6 Febiger, Philadelphia.
Τσακάλωφ Π. Μαιευτική των κατοικίδιων θηλαστικών. ΑΠΘ 1986.
Stefani JS, Palha MDC, Christmann L, Rosa JM. Laparoscopie versus surgical transfer of ovine embryos. Theriogenology 1990,33,330.
Besenfelder U. Hazas G, Gyoker E, Gergatz E and Brem G. Laparoscopie embryo transfer into the fallopian tube in sheep. Theriogenology 1994,41,162.
Most read articles by the same author(s)