Isolation of a sheep - embryo after oestrus synchronisation


Ν. Τσερνενές
Β. Λαλιώτης
Σ. Ποΐλας
Abstract

The Center of Artificial Insemination and Reproductive Diseases of Athens pursuing the embryotraslantation in the productive Animals began an experimental work of the whole subject. Subject of the this study was, as a first step, the isolation of sheep - embryo. The research was done in two sheep - hydrld after synchromistation of oestrus by PMSG and prostaglandin. The isolation of the embryos was achieved by repeated washings of the horns of uterus with Dulbecco' s solution after laparotomy at seventh days post inoculation of P.M.S.G. The washinliquide were examined at the steroscope, one embryo was isolated and after was photographed. The embryo was at the stage of morula which coinsides to the 5t h day of the natural mating.

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References
MOORE, Ν. W. & SHELTON, J. Ν. (1964) Egg transfer in sheep. Effect of synchronization between donor and recipient, age of egg, and site of transfer on the survival of transferred eggs. J. Reprod. Fert. 7,145.
ROWSON, L. E. A. & MOOR, R. M. (1966) Embryo transfer in the sheep: the significance of synchronizing oestrus in the donor and recipient animal. J. Reprod. Fert. 11,207 - 212.
HUNTER, G. L, ADAMS, C. E. & ROWSON, L E. A. (1955) Interbreed ovum transfer in sheep. J. Agric. Sci. 46,143.
ARMSTRONG, D. Τ, PFITZNER, Α. Ρ, WARNES, G. M, RALPH, Μ. Μ, & SEAMARK, R.F. (1983). Endocrine responses of goats after induction of superovulation with PMSG and FSH. J. Reprod. Fert. 67, 395.
ARNSTRONG, D. T, PFITZNER, A. R, WARNES, G. M, & SEAMARK, R. F (1983) Superovulation treatments and embryo transfer in Angora goats.
KARG H. D. SCHAMS, Β HOFFMANN R. CLAUS. New Erkenntnisse der Endokrinologie der Fortpflanzung (Der Praktische Tierarzt 7,1979).
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