Effect of iron oxide and silver nanoparticles on boar semen CASA motility and kinetics

PDF
DOI: https://doi.org/10.12681/jhvms.25084
Keywords:
boar semen CASA motility Nanoparticles
A. BASIOURA
Farm Animals Clinic, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Greece
I. MICHOS
Farm Animals Clinic, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Greece
A. NTEMKA
Farm Animals Clinic, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Greece
I. KARAGIANNIS
Farm Animals Clinic, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Greece
C.M. BOSCOS
Farm Animals Clinic, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Greece
Abstract

The objective of the study was to investigate the potential toxic effect of iron oxide (Fe3O4) and silver (Ag/Fe) spherical nanoparticles (NPs) as alternative antimicrobial compounds on boar semen. The NPs’ minimum inhibitory concentration was determined applying the in vitro antimicrobial activity evaluation test and included in the experiment. Totally, 9 ejaculates (3 boars; 3 ejaculates/boar) were extended in BTS without antibiotics at 30×106 spermatozoa/mL and divided in 3 aliquots corresponding to the following groups: 1) Control group (C): extended semen without treatment; 2) Iron oxide group (Fe): extended semen with Fe3O4 NPs of diameter 40 nm (0.192 mg/mL semen); and 3) Silver group (Ag): extended semen with Ag/Fe NPs of diameter 30 nm, consisted of Ag and a 5% of zero-valent Fe (0.128 mg/mL semen). Semen samples of all groups were incubated at 17o C for 30 min following NPs’ removal through a magnetic field. All post treated samples were stored at 17o C for 48 h. Total motility (TM) and kinetics (progressive motility PM; rapid/medium/slow movement spermatozoa; static spermatozoa; VCL; VSL; VAP; LIN; STR; WOB; ALH; BCF; hyperactive spermatozoa) were evaluated by CASA system at 0, 24 and 48 h post treatment. Data were analyzed with a repeated measures mixed model. Group Fe did not differ from group C at any time point. TM and PM were lower at 24 h of storage in group Ag compared to groups C and Fe (all P<0.001). By 48 h of storage spermatozoa of group Ag were totally immotile and thus excluded from analysis. The comparison within groups and between storage time points showed that the values of TM, PM, VCL, VAP, ALH and BCF decreased after 24 h of storage in group Ag (all P<0.05), but not in groups C and Fe, while no significant differences were observed for the remaining parameters between successive time points within any group (P>0.05). In conclusion, Ag/Fe NPs demonstrated a harmful effect on boar spermatozoa, while the used concentration of the examined Fe3O4 NPs did not affect boar sperm CASA motility parameters enhancing further research about their application on semen handling.

Article Details
  • Section
  • Research Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Authors who publish with this journal agree to the following terms:

· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Downloads
Download data is not yet available.
References
Afifi M, Almaghrabi OA, Kadasa NM (2015) Ameliorative Effect of Zinc Oxide Nanoparticles on Antioxidants and Sperm Characteristics in Streptozotocin-Induced Diabetic Rat Testes. Biomed Res. Int.:16.
Akhter S, Ansari MS, Andrabi SMH, Ullah N, Qayyum M (2008) Effect of antibiotics in extender on bacterial and spermatozoal quality of cooled buffalo (Bubalus bubalis) bull semen. Reprod. Domest. Anim. 43:272278.
Althouse GC, Kuster CE, Clark SG, Weisiger RM (2000) Field investigations of bacterial contaminants and their effects on extended porcine semen. Theriogenology 53:11671176.
Asare N, Instanes C, Sandberg WJ, Refsnes M, Schwarze P, Kruszewski M, Brunborg G (2012) Cytotoxic and genotoxic effects of silver nanoparticles in testicular cells. Toxicology 291:6572.
Azam A, Ahmed AS, Oves M, Khan MS, Habib SS, Memic A (2012) Antimicrobial activity of metal oxide nanoparticles against Gram-positive and Gram-negative bacteria: a comparative study. Int. J. Nanomedicine 7:60039.
Azawi OI, Ismaeel MA (2012) Influence of addition of different antibiotics in semen diluent on viable bacterial count and spermatozoal viability of Awassi ram semen. Vet. World 5:7579.
Babes L, Denizot B, Tanguy G, Le Jeune JJ, Jallet P (1999) Synthesis of iron oxide nanoparticles used as MRI contrast agents: A parametric study. J. Colloid Interface Sci. 212:474482.
Barkalina N, Jones C, Kashir J, Coote S, Huang X, Morrison R, Townley H, Coward K, (2014) Effects of mesoporous silica nanoparticles upon the function of mammalian sperm in vitro. Nanomedicine Nanotechnology, Biol. Med. 10:859870.
Barkhordari A, Hekmatimoghaddam S, Jebali A, Khalili MA, Talebi A, Noorani M (2013) Effect of zinc oxide nanoparticles on viability of human spermatozoa. Iran. J. Reprod. Med. 11:767771.
Braydich-Stolle L, Hussain S, Schlager JJ, Hofmann MC (2005) In vitro cytotoxicity of nanoparticles in mammalian germline stem cells. Toxicol. Sci. 88:412419.
Burdușel AC, Gherasim O, Grumezescu AM, Mogoantă L, Ficai A, Andronescu E (2018) Biomedical Applications of Silver Nanoparticles: An Up-to-Date Overview. Nanomaterials 8.
Chaloupka K, Malam Y, Seifalian AM (2010) Nanosilver as a new generation of nanoproduct in biomedical applications. Trends Biotechnol. 28:580588.
Durfey CL, Burnett DD, Liao SF, Steadman CS, Crenshaw MA, Clemente HJ, Willard ST, Ryan PL, Feugang JM (2017) Nanotechnology-based selection of boar spermatozoa: growth development and health assessments of produced offspring. Livest. Sci. 205:137142.
Durfey CL, Swistek SE, Liao SF, Crenshaw MA, Clemente HJ, Thirumalai
RVKG, Steadman CS, Ryan PL, Willard ST, Feugang JM (2019) Nanotechnology-
based approach for safer enrichment of semen with best spermatozoa.
J. Anim. Sci. Biotechnol. 10:14.
Ema M, Kobayashi N, Naya M, Hanai S, Nakanishi J (2010) Reproductive and developmental toxicity studies of manufactured nanomaterials. Reprod. Toxicol. 30:343352.
Falchi L, Galleri G, Dore GM, Zedda MT, Pau S, Bogliolo L, Ariu F, Pinna A, Nieddu S, Innocenzi P, Ledda S 2018 Effect of exposure to CeO2 nanoparticles on ram spermatozoa during storage at 4 °C for 96 hours. Reprod. Biol. Endocrinol. 16:19.
Feugang J, Liao S, Crenshaw M, Clemente H, Willard S, Ryan P (2015) Lectin-functionalized magnetic iron oxide nanoparticles for reproductive improvement. JFIV Reprod Med Genet. 3:17-19.
Garcia TX, Costa GMJ, França LR, Hofmann MC (2014) Sub-acute intravenous administration of silver nanoparticles in male mice alters Leydig cell function and testosterone levels. Reprod. Toxicol. 45:5970.
Grade S, Eberhard J, Neumeister A, Wagener P, Winkel A, Stiesch M, Barcikowski S, (2012) Serum albumin reduces the antibacterial and cytotoxic effects of hydrogel-embedded colloidal silver nanoparticles. RSC Adv. 2:71907196.
Gromadzka-Ostrowska J, Dziendzikowska K, Lankoff A, Dobrzyńska M, Instanes C, Brunborg G, Gajowik A, Radzikowska J, Wojewódzka M, Kruszewski M (2012) Silver nanoparticles effects on epididymal sperm in rats. Toxicol. Lett. 214:251258.
Gyles C (2011) The growing problem of antimicrobial resistance. Can. Vet. J. La Rev. Vet. Can. 52:817820.
Hajipour MJ, Fromm KM, Ashkarran AA, de Aberasturi DJ, de Larramendi IR, Rojo T, Serpooshan V, Parak WJ, Mahmoudi M (2012) Antibacterial properties of nanoparticles. Trends in biotechnology 30:499-511.
Johnston HJ, Hutchison G, Christensen FM, Peters S, Hankin S, Stone V (2010) A review of the in vivo and in vitro toxicity of silver and gold particulates: Particle attributes and biological mechanisms responsible for the observed toxicity. Crit. Rev. Toxicol. 40:328346.
Karageorgiou MA, Tsousis G, Boscos CM, Tzika ED, Tassis PD, Tsakmakidis IA (2016) A comparative study of boar semen extenders with different proposed preservation times and their effect on semen quality and fertility. Acta Vet Brno. 85:23-31.
Lafuente D, Garcia T, Blanco J, Sánchez DJ, Sirvent JJ, Domingo JL, Gómez M (2016) Effects of oral exposure to silver nanoparticles on the sperm of rats. Reprod. Toxicol. 60:133139.
Lan Z, Yang WX (2012) Nanoparticles and spermatogenesis: How do nanoparticles affect spermatogenesis and penetrate the blood-testis barrier. Nanomedicine. 7:579596.
Lemire JA, Harrison JJ, Turner RJ (2013) Antimicrobial activity of metals: mechanisms, molecular targets and applications. Nat. Rev. Microbiol. 11:371384.
Lin CX, Yang SY, Gu JL, Meng J, Xu HY, Cao JM (2017) The acute toxic effects of silver nanoparticles on myocardial transmembrane potential, Ina and IK1 channels and heart rhythm in mice. Nanotoxicology 11:827837.
Moretti E, Terzuoli G, Renieri T, Iacoponi F, Castellini C, Giordano C, Collodel G, (2013) In vitro effect of gold and silver nanoparticles on human spermatozoa. Andrologia 45:392396.
Odhiambo JF, DeJarnette JM, Geary TW, Kennedy CE, Suarez SS, Sutovsky M, Sutovsky P (2014) Increased Conception Rates in Beef Cattle Inseminated with Nanopurified Bull Semen1. Biol. Reprod. 91.
Özgür M, Ulu A, Balcıoğlu S, Özcan İ, Köytepe S, Ateş B, Özgür ME, Ulu A, Balcıoğlu S, Özcan İ, Köytepe S, Ateş B (2018) The Toxicity Assessment of Iron Oxide (Fe3O4) Nanoparticles on Physical and Biochemical Quality of Rainbow Trout Spermatozoon. Toxics 6:62.
Raana J, Parisa Y, Mehdi AA, Abdollah MS, Hamid V, Mohammad C, Hasan NM, Reza BM (2015) Effect of zinc nano-complex on bull semen quality after freeze-thawing process. J. Anim. Prod. (Journal Agric.) 17:371380.
Rai M, Yadav A, Gade A (2009) Silver nanoparticles as a new generation of antimicrobials. Biotechnol. Adv. 27:7683.
Ribeiro APC, Anbu S, Alegria ECBA, Fernandes AR, Baptista PV, Mendes R, Matias AS, Mendes M, Guedes da Silva MFC, Pombeiro AJL (2018) Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles. Biomed. Pharmacother. 101:137144.
Safa S, Moghaddam G, Jozani RJ, Daghigh Kia H, Janmohammadi H (2016) Effect of vitamin E and selenium nanoparticles on post-thaw variables and oxidative status of rooster semen. Anim. Reprod. Sci. 174:100106.
Salamon S, Maxwell WM (2000) Storage of ram semen. Anim. Reprod. Sci. 62:77111.
Salata O (2004) Applications of nanoparticles in biology and medicine. J. Nanobiotechnology 2:3.
Stensberg MC, Wei Q, McLamore ES, Porterfield DM, Wei A, Sepúlveda MS (2011) Toxicological studies on silver nanoparticles: Challenges and opportunities in assessment, monitoring and imaging. Nanomedicine. 6:879898.
Terzuoli G, Iacoponi F, Moretti E, Renieri T, Baldi G, Collodel G (2012) In vitro effect of silver engineered nanoparticles on human spermatozoa. J. Siena Acad. Sci. 3:27.
Tiedemann D, Taylor U, Rehbock C, Jakobi J, Klein S, Kues WA, Barcikowski S, Rath D (2014) Reprotoxicity of gold, silver, and gold-silver alloy nanoparticles on mammalian gametes. Analyst 139:931942.
Varner DD, Scanlan CM, Thompson JA, Brumbaugh GW, Blanchard TL, Carlton CM, Johnson L (1998) Bacteriology of preserved stallion semen and antibiotics in semen extenders. Theriogenology 50:559573.
Varzeghani SM, Parivar K, Abdollahifar MA, Karamian A (2018) Effects of Iron Oxide Nanoparticles on Mouse Sperm Parameters and Testicular Tissue, Iranian Journal of Toxicology. Iranian Journal of Toxicology. 12:3944.
Wiwanitkit V, Sereemaspun A, Rojanathanes R (2009) Effect of gold nanoparticles on spermatozoa: the first world report. Fertil. Steril. 91:e7.
Most read articles by the same author(s)