Effects of dietary aspirin supplementation on liver enzymes, immune response, cecum microflora and fatty acids profile in breast meat of broiler chickens

PDF
Published: Nov 9, 2022
Updated: 2022-11-09
DOI: https://doi.org/10.12681/jhvms.27695
Keywords:
aspirin broilers blood parameters fatty acids profile immune system
M Tavakoli
Department of Animal Science, Rasht Branch, Islamic Azad University, Rasht, Iran
https://orcid.org/0000-0002-0051-1818
M Bouyeh
Department of Animal Science, Rasht Branch, Islamic Azad University, Rasht, Iran
https://orcid.org/0000-0002-2622-3777
A Seidavi
Department of Animal Science, Rasht Branch, Islamic Azad University, Rasht, Iran
https://orcid.org/0000-0002-1903-2753
Abstract

The present study was performed to investigate the effect of dietary aspirin (A) on broilers. A completely randomized design using 120 one-day-old male broiler chicks Ross 308, three levels of aspirin (0, 50, and 100 mg/kg) was used in 4 replicates, each including 10 chicks, during 42 days. The effects of different levels of aspirin, added to a basal diet, on blood plasma parameters, liver enzymes, immune system, cecal microflora, and fatty acids profile of breast muscle of chicks were investigated. Data analysis was performed by SAS statistical software and the comparison of the means with Duncan's test. The results showed that the effects of aspirin on blood parameters, liver enzymes of broilers (except for alkaline phosphatase which significantly reduced), the humoral immune system in response to antigen injection as sheep red blood cell (SRBC), antibody titer against Newcastle and influenza virus were not significant (P≥0.05). The highest percentage of neutrophils and eosinophils was related to this treatment. Also, aspirin treatments increased numerically the levels of unsaturated fatty acids and decreased saturated fatty acids. Moreover, aspirin led to a reduction in the population of Escherichia coli. So, based on the results of the present study, the use of 100 mg/kg aspirin in the diet of broilers is recommendable.

Article Details
  • Section
  • Research Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Authors who publish with this journal agree to the following terms:

· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Downloads
Download data is not yet available.
Author Biographies
M Tavakoli, Department of Animal Science, Rasht Branch, Islamic Azad University, Rasht, Iran

PhD Candidate

M Bouyeh, Department of Animal Science, Rasht Branch, Islamic Azad University, Rasht, Iran

Assistant professor

A Seidavi, Department of Animal Science, Rasht Branch, Islamic Azad University, Rasht, Iran

Professor

References
Abdulameer, YS. 2019. The effects of dietary vitamin C and Citrus sinensis peel on growth, hematological characteristics, immune competence, and carcass characteristics in broilers exposed to heat stress.Iraqi Journal of Veterinary Sciences32(2): 253-260.
Alagawany, M., Farag, MR., El-Hack,MA., Dhama, K., and J. Fowler. 2017. Use of acetylsalicylic acid as a feed additive in poultry nutrition. World's Poultry Science Journal73(3): 633-642.
Dibaji, SM., Seidavi, A., L. Asadpour and F. Moreira da Silva. 2014. Effect of a synbiotic on the intestinal microflora of chickens. Journal of Applied Poultry Research23(1): 1-6.
Folch, J., Lees, M., and GS.Stanley. 1957. A simple method for the isolation and purification of total lipides from animal tissues. Journal of biological chemistry 226(1): 497-509.
Ghalib, R., Falah, A, and A. Ahmad.2011.Ulcerogenic effects of NSAIDs on gastric mucosa: comparison between selective and non-selective COX2 inhibitors; indomethacin VS rofecoxib. Medical Journal of Babylon 8(2):197-205.
Golrokh,AJ., Bouyeh, M., Seidavi, A., van den Hoven, R., Laudadio, V, and V.Tufarelli.2016.Effect of different dietary levels of atorvastatin and L-carnitine on performance, carcass characteristics and plasma constituites of broiler chickens. The Journal of Poultry Science53(3): 201-207.
Gore, AB, and MA.Qureshi. 1997.Enhancement of humoral and cellular immunity by vitamin E after embryonic exposure. Poultry Science 76(7): 984-991.
Huang, YS., Huang, WC., CV Li, and LT. Chuang. 2011. Eicosadienoic acid differentially modulates production of pro-inflammatory modulators in murine macrophages. Molecular and cellular biochemistry 358(1-2): 85-94.
Jang, IS., Ko,YH., SY Kang, and CY. Lee.2007. Effect of a commercial essential oil on growth performance, digestive enzyme activity and intestinal microflora population in broiler chickens. Animal Feed Science and Technology 134(3-4): 304-315.
Krauss, RM.2004. Hold the antioxidants and improve plasma lipids? The Journal of Clinical Investigation 113(9):1253-1255.
Lerner, KG., B Glick,and FC. McDuffie.1971. Role of the bursa of Fabricius in IgG and IgM production in the chicken: evidence for the role of a non-bursal site in the development of humoral immunity. The Journal of Immunology 107(2): 493-503.
Madamanchi, NR. A., Vendrov, and MS, Runge.2005.Oxidative stress and vascular disease.Arteriosclerosis, Thrombosis, and Vascular Biology25(1): 29-38.‏
Manual, RB. 2012. Ross 308 broiler nutrition manual. Ross Broiler Ltd: Newbridge, Midlothian, UK,
Nowak, JZ. 2014. Aspirin and age-related macular degeneration: positives versus negatives. Expert Opinion on Drug Safety 13(6): 687-690.
Ocak, N., Erener, G., Burak, AkF., Sungu, M., A Altop,and A. Ozmen. 2008. Performance of broilers fed diets supplemented with dry peppermint (Menthapiperita L.) or thyme (Thymus vulgaris L.) leaves as growth promoter source.Czech Journal of Animal Science53(4): 169.‏
Pourhossein, Z., Qotbi, AAA., Seidavi, A., Laudadio, V., G Centoducati, and V. Tufarelli. 2015. Effect of different levels of dietary sweet orange (Citrus sinensis) peel extract on humoral immune system responses in broiler chickens. Animal Science Journal86(1): 105-110.
Poźniak, B. Yuan, H., Yi, J., Guo, C., Deng, S., L, Zhu., and M.Świtała. 2010. Influence of salicylate administration on weight gain and serum biochemical parameters in broiler chickens. MedycynaWeterynaryjna 66(10): 675-679.
Richard, LM. 2007. The 100 most important chemical compounds: a reference guide. ABC-CLIOp. 10.ISBN 978-0-313-33758-1.
Rokade,JJ.,Bhanja, SK., Shinde, AS., DB Bhaisare,and AB. Mandal.2017. Evaluation of aspirin(ASA) in broiler chicken during hot dry summer using zoo technical, molecular and physio-biochemical tools. Indian Journal of Animal Research 51(1): 97-104.
Sahin, K., Orhan, C., MO, Smith., and N.Sahin. 2013. Molecular targets of dietary phytochemicals for the alleviation of heat stress in poultry.World's Poultry Science Journal69(1): 113-124.‏
Sahin, K., N. Sahin., and S.Yaralioglu. 2002. Effects of vitamin C and vitamin E on lipid peroxidation, blood serum metabolites, and mineral concentrations of laying hens reared at high ambient temperature. Biological Trace Element Research 85(1); 35-45.
Sahin, K., Sahin, N., Onderci, M., MF. Gursu., and M. Issi. 2003. Vitamin C and E can alleviate negative effects of heat stress in Japanese quails. Food, Agriculture and Environment 1(2): 244-49.
Sahin, K. 2015. Modulation of NF-κB and Nrf2 pathways by lycopene supplementation in heat-stressed poultry. World's Poultry Science Journal 71(2): 271-284.
Seidavi, A., Asadpour, L., M Dadashbeiki,and R.Payan-Carreira.2014. Effects of dietary fish oil and green tea powder supplementation on broiler chickens’ immunity. Acta Scientiae Veterinariae 42(1): 1-13.
Shabani, S., Seidavi, A., L Asadpour, and M.Corazzin. 2015. Effects of physical form of diet and intensity and duration of feed restriction on the growth performance, blood variables, microbial flora, immunity, and carcass and organ characteristics of broiler chickens. Livestock Science180: 150-157.
Truong, A., Sayago,MM.,WH Kutteh,and RW.Ke.2016.Subchorionic hematomas are increased in early pregnancy in women taking low-dose aspirin. Fertility and Sterility 105(5): 1241-1246.
Tras, B., Inal, F., Bas, AL., Altunok, V., Elmas, M,andYazar, E. 2000. Effects of continuous supplementations of ascorbic acid, aspirin, vitamin E and selenium on some haematological parameters and serum superoxide dismutase level in broiler chickens. British poultry science41(5): 664-666.‏
Valko, MMHCM., H Morris, and MTD. Cronin. 2005. Metals, toxicity and oxidative stress. Current Medicinal Chemistry 12(10): 1161-1208.
Wang, L., Piao,XL., Kim, SW., Piao, XS., YB Shen, andHS.Lee.2008. Effects of Forsythia suspensa extract on growth performance, nutrient digestibility, and antioxidant activities in broiler chickens under high ambient temperature.Poultry Science87(7); 1287-1294.‏
Wang, X., H. Lin., and Y. Gu. 2012. Multiple roles of dihomo-γ-linolenic acid against proliferation diseases. Lipids in Health and Disease 11(1): 25.
Whiting, TL., ME. Drain and Dp. Rasali.2007.Warm weather transport of broiler chickens in Manitoba. II. Truck management factors associated with death loss in transit to slaughter. The Canadian Veterinary Journal 48(2): 148.
Wu, D., Zhang, M., Lu, Y., Tang, S., Kemper, N., J Hartung, and E.Bao.2016. Aspirin-induced heat stress resistance in chicken myocardial cells can be suppressed by BAPTA-AM in vitro. Cell Stress and Chaperones21(5): 817-827.
Most read articles by the same author(s)