Quality aspects of oven-dried whole (full-fat) and d-oiled mealworm stored at room temperature


Published: May 1, 2024
Keywords:
Tenebriomolitor nutrient composition microbial load aflatoxin B1
SZ Khan
A Usman
K Khan
https://orcid.org/0000-0002-6255-2842
P Ali
AA Shah
H Khan
M Israr
Abstract

Mealworms (Tenebrio molitor) are considered the potential novel alternative source of protein for sustainable food production and also have a low ecological footprint. Nonetheless, fresh mealworms have a shorter shelf-life, therefore, to preserve their nutrient quality and safety it is of utmost importance to optimize the post-harvest processing techniques. Therefore, in the present study, two technological forms of oven-dried (60 0Ċ for 2 h) mealworms namely whole (full-fat) mealworms (WMW) and de-oiled mealworms (D-OMW) were analysed for nutrient quality, microbial count, and aflatoxin B1 safety levels. Both WMW and D-OMW were stored for 56 days at room temperature (25 to 30 0C) and were sampled on alternate weeks (days 0, 14, 28, 42, and 56, respectively) for analysis. In comparison to WMW, the D-OMW contains high (P < 0.001) contents of crude protein (CP) and dry matter (DM). Notably, at 56 days of storage interval, the D-OMW is more stable with CP and DM contents having DM (87.3 vs. 77.3%) and CP (76 vs. 46.0%) contents. In contrast, WMW had high (P < 0.001) content of EE than D-OMW (24.5 vs. 3.30%) at 56 days of storage. Moreover, WMW and D-OMW are rich sources of minerals, particularly potassium and phosphorus, with no significant (P > 0.05) effect during storage interval. Notably, leucine, lysine, and valine are the abundant amino acids in WMW and D-OMW. During the entire storage period, no growth of Escherichia coli, Salmonella, Campylobacters, and Clostridium perfringens were found. However, low levels of other coliforms, Enterococci, and Staphylococcus aureus were detected in D-OMW than in WMW. Likewise, the aflatoxin B1 contamination level was much lower in D-OMW during the entire storage time. This study concluded that storage times affected the nutrient profile and safety level of WMW. Nonetheless, the D-OMW was found almost stable and safe even at the storage of 56 days at room temperature.

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References
AOAC (1990). Methods of Analysis of the Association of Official Analytical Chemists, Association of Official Analytical Chemists, Washington, DC, USA, 1990.
AOAC (1995).Association of Official Analytical Chemists, 16thed. Arlington, VA, USA.
BonazziC, DumoulinE(2011). Quality changes in food materials as influenced by drying processes. Modern Dry Technol 3: 1-20.
Bosch G, van der Fels-Klerx HJ, de Rijk TC, Oonincx DGAB(2017). Aflatoxin B1 tolerance and accumulation in black soldier fly larvae
(Hermetia illucens) and yellow mealworms (Tenebrio molitor). Toxins 9: 185. https://doi.org/10.3390/toxins9060185
BukkensSGF (2005). Insects in the human diet: nutritional aspects. Ecological implications of mini livestock, the role of rodents, frogs, snails, and insects for sustainable development.Science Publishers, New Hampshire. 545-577.
ChakravarthyAK, JayasimhaGT, Rachana RR, Rohini G(2016). Insects as human food. In: Chakravarthy A, Sridhara S, (eds) Economic
and ecological significance of arthropods in diversified ecosystems. Springer, Singapore, 133-146. https://doi.org/10.1007/978-981-10-1524-3_7
De Marcoa M, Martinez S, Hernandez F, Madrid J, Gai F, Rotolo L, Belforti M, Bergeroa D, Katz H, Dabboud S, Kovitvadhi A, Zoccarato I, Gascoc L, Schiavone A(2015). Nutritional value of two insect larval meals (Tenebrio molitor and Hermetia illucens) for broiler chickens: apparent nutrient digestibility, apparent ileal amino acid digestibility and apparent metabolizable energy. Anim Feed SciTechnol 209: 211-218.
Ravzanaadii N, Kim SH, Choi WH, Hong SJ, Kim NJ(2012).Nutritional value of mealworm, Tenebrio molitor as food source.Int JIndustEntomol 25(1): 93-98.
EFSA Scientific Committee (2015). Risk profile related to production and consumption of insects as food and feed. EFSA Journal 13(10): 4257. https://doi.org/10.2903/j.efsa.2015.4257
FAO (2012).Assessing the potential of insects as food and feed in assuring food security. Food and Agriculture Organization of the United Nations (FAO), Summary Report, Rome, Italy, 1: 1-38.
FAO (2013). Edible insects: future prospects for food and feed security. Food and Agriculture Organization of the United Nations, (FAO) Rome, Italy.http://www.fao.org/ i3253e.pdf
FinkeMD(2002). Complete nutrient composition of commercially raised invertebrates used as food for insectivores. Zoo Biology: Published in Affiliation with the American Zoo and Aquarium Association 21(3): 269-285.
Parniakov O,Mikhrovska M,Wiktor A, Alles M, Ristic D, BoguszR, Nowacka M, Devahastin S, Mujumdar A, Heinz V, Smetana S
(2022). Insect processing for food and feed: A review of drying methods. Drying Technology, 40(8), 1500-1513.
Hernandez-Alvarez AJ, Mondor M, Pina-Dominguez IA, Sanchez-Velazquez OA, MelgarLalanne G (2021). Drying technologies
for edible insects and their derived ingredients. Drying Technology, 39(13), 1991-2009.
GilbertJ, AnklamE (2002). Validation of analytical methods for determining mycotoxins in foodstuffs. Trends Anal Chem 21(6-7): 468-86.
Grabowski NT,Klein G(2017). Bacteria encountered in raw insect, spider, scorpion, and centipede taxa including edible species, and their significance from the food hygiene point of view. Trends Food SciTechnol 63: 80-90.
HussainI, KhanS, SultanA, ChandN, KhanR, AlamW, AhmadN (2017). Mealworm (Tenebrio molitor) as a potential alternative source of protein supplementation in broiler. Int JBiosci 10(4): 225-262.
Jin XH, Heo PS, Hong JS, Kim NJ, Kim YY (2016). Supplementation of dried mealworm (Tenebrio molitor larva) on growth performance, nutrient digestibility and blood profiles in weaning pigs. Asian-Australas J AnimSci 29: 979-986.
KartsovaLA, KorolevaOA(2007). Simultaneous determination of water and fat-soluble vitamins by high-performance thin-layer chromatography using an aqueous micellar mobile phase. J Anal Chem62: 255-259.
Khan S, Khan RU, AlamW, SultanA(2018).Evaluating the nutritive profile of three insect meals and their effects to replace soya bean in broiler diet. J animphysiolanimnutr 102(2): e662-e668.
Khan S, Khan RU, Sultan A, Khan M, Hayat SU, Shahid MS(2016).Evaluating the suitability of maggot meal as a partial substitute of soya bean on the productive traits, digestibility indices and organoleptic properties of broiler meat.J AnimPhysiolAnimNutr 100: 649-656.
Khan K, Khan S, Ullah S, Khan NA, Khan I, Ahmad N(2017). Nutritive value, fiber digestibility and methane production potential of tropical forages in rabbits: effect of species and harvest maturity. J AnimPlant Sci 27: 1094-1100.
Klunder HC, Wolkers-Rooijackers J, Korpela JM, Nout MJR(2012).Microbiological aspects of processing and storage of edible insects. Food Control 26: 628-631.
Kroncke N, Grebenteuch S, Keil C, Demtroder S, Kroh L, Thunemann AF, Benning R, Haase H (2019). Effect of different drying methods on nutrient quality of the yellow mealworm (Tenebrio molitor L.). Insects 10: 84.
Makkar PSH, Tran G, Heuze V, Ankers P (2014). State of the art on use of insects as animal feed.Anim Feed SciTechnol, 197: 1-33.
Nowak V, Persijn D, Rittenschober D, Charrondiere UR (2016). Review of food composition data for edible insects. Food Chem 193: 39-46.
Oonincx DGAB, Van Itterbeeck J, Heetkamp MJW, Van den Brand H, Van Loon JJA, Van Huis A (2010). An exploration on greenhouse gas and ammonia production by insect species suitable for animal or human consumption.PLoS One 5(12): e14445.
Ramashia SE, Tangulani T, Mashau ME, Nethathe B(2020). Microbiological quality of different dried insects sold at Thohoyandou open market, South Africa. Food Res 4: 2247-2255.
RumpoldBA, SchluterOK(2013).Nutritional composition and safety aspects of edible insects.MolNutr Food Res 57: 802-823.
Shang-GuiD, Zhi-Ying P, Fang C, Ping Y, Tie W(2004).Amino acid composition and anti-anaemia action of hydrolyzed offal protein from Harengula Zunasi Bleeker. Food Chem 87(1): 97-102.
Stastnik O, Novotny J, Roztocilova A, Kouril P, KumbarV, Cernik J, Kalhotka L, Pavlata L, Lacina L, Mrkvicova E(2021). Safety of meal worm meal in layer diets and their influence on gut morphology.Anim 11: 1439.
Swick MC, Koehler TM, Driks A(2016). Surviving between hosts: sporulation and transmission. Microbiolspectr 4(4): 567-591.
Van HuisA(2013). Potential of insects as food and feed in assuring food security.AnnuRevEntomol 58: 563-583.
VandeweyerD, LenaertsS, CallensA, Van CampenhoutL(2017). Effect of blanching followed by refrigerated storage or industrial microwave drying on the microbial load of yellow mealworm larvae (Tenebrio molitor). Food Contr 71: 311-314
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