In vitro investigation of the efficacy of myo-inositol (vitamin B8) as an antioxidant and preservative additive for feed safety
Abstract
The study was carried out to examine the safety and potential activities of myo-inositol as an antioxidant, preservative, hygiene enhancer, and antiviral feed additive in pet nutrition. For this purpose, the antioxidant capacity of myo-inositol was expressed as vitamin C equivalent antioxidant capacity (VCEAC) by MTT assay. The cytotoxic concentration of 50% (CC50) was measured by the linearity between myo-inositol concentrations and the viability of mammalian kidney and liver cell lines. The virucidal and antiviral activities of myo-inositol were determined by the virus titration method on the host cell infectivity of murine norovirus-1 (MNV-1) and expressed as the tissue cell infective dose of 50% using the Spearman–Karber method. One milligram of myo-inositol equals the antioxidant capacity of 0.454 mg of vitamin C. The CC50 was determined between 280 and 966 mg/ml for kidney and liver cell cultures. Myo-inositol exhibited dose-dependently virucidal activity against MNV-1 as a surrogates for pet caliciviruses. In three different antiviral experiments, 50% inhibition concentrations were determined as 0.054, 0.084, and 0.047 mg/ml. This in vitro study presents that myo-inositol might be a potential candidate as an antioxidant, preservative, and hygiene enhancer feed additive against oxidative deterioration and microbial contamination in feed production technology for ensuring feed safety and promoting pet health.
Article Details
- How to Cite
-
Korkmaz, S., Sait, A., & Keleş, A. (2025). In vitro investigation of the efficacy of myo-inositol (vitamin B8) as an antioxidant and preservative additive for feed safety. Journal of the Hellenic Veterinary Medical Society, 76(1), 8597–8606. https://doi.org/10.12681/jhvms.35879
- Issue
- Vol. 76 No. 1 (2025)
- Section
- Research Articles
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
