Valuation of three different anaesthetic protocols on complete blood count and biochemical parameters on Wistar rats

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DOI: https://doi.org/10.12681/jhvms.16057
P. P. LELOVAS
Center of Clinical, Experimental Surgery and Translational Research, Laboratory Animal Unit, Biomedical Research Foundation, Academy of Athens
M. S. STASINOPOULOU
Center of Clinical, Experimental Surgery and Translational Research, Laboratory Animal Unit, Biomedical Research Foundation, Academy of Athens
E. G. BALAFAS
Center of Clinical, Experimental Surgery and Translational Research, Laboratory Animal Unit, Biomedical Research Foundation, Academy of Athens
M. A. NIKITA
Center of Clinical, Experimental Surgery and Translational Research, Laboratory Animal Unit, Biomedical Research Foundation, Academy of Athens
N. T. SISKOS
Center of Clinical, Experimental Surgery and Translational Research, Laboratory Animal Unit, Biomedical Research Foundation, Academy of Athens; Laboratory of Anatomy, Histology and Embryology, Veterinary School, Faculty of Health Sciences, Aristotle University of Thessaloniki
N. G. KOSTOMITSOPOULOS
Center of Clinical, Experimental Surgery and Translational Research, Laboratory Animal Unit, Biomedical Research Foundation, Academy of Athens
Abstract

The objective of the current study was to determine the impact of three different anaesthetic protocols on blood and biochemical parameters. Eighteen female Wistar rats (HsdOla:WI) 3 months old, weighting 197.09 ± 7.39 g were used. Baseline blood collection was performed in all animals from the lateral coccygeal vein for evaluation of glucose. The animals were then randomly allocated to receive one of three different anaesthetic protocols: dexmedetomidine/ketamine intramuscularly (0.25 mg/kg and 50 mg/kg respectively), or isoflurane 0.2 ml on cotton inside a syringe case, or isoflurane administered by vaporiser (5% induction and 2-3% maintenance of anaesthesia, delivered in oxygen flow 1 L/min). Blood samples were collected from caudal vena cava for complete blood count and biochemical analysis, while the lung and the liver were harvested for histological evaluation. Comparison between groups, as far as complete blood count parameters is concerned, revealed statistical significant differences in red blood cells, haematocrit and haemoglobin values with lower values being observed in vaporiser anaesthesia group. Furthermore, within group comparison revealed statistical significant differences for glucose in all three groups. No evidence of inflammatory, degenerative neoplastic or toxicity related lesions were observed during histological evaluation of the lung and liver.

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Literaturhinweise
Anonymous.(2012). Isoflurane [MAK Value Documentation, 1996]. The MAK Collection for Occupational Health and Safety. pp 128–140.
Anonymous (1998) Baseline Hematology and Clinical Chemistry Values for Charles River Wistar Rats (CRL: (WI)BR) as a function of Sex and Age. Technical bulletin, Charles River Laboratories, Wilmington.
Balafas E, Papastefanou A, Katsimpoulas M, Kostomitsopoulos N (2011) A low cost face mask for inhalation anaesthesia in rats. Scand J Lab Anim Sci 38:111-115.
Bihun G, Bauck L (2004) Basic Anatomy, Physiology, Husbandry and Clinical Techniques. Ferrets, Rabbits and Rodents. In: Animal Medicine and Surgery. 2nd ed, Elsevier, St Louis, Misouri:pp 286-298.
Brown ET, Umino Y, Loi T, Solessio E, Barlow R (2005) Anesthesia can cause sustained hyperglycemia in C57/BL6J mice. Vis Neurosci. 22:615-618.
Brunson DB (1997) Pharmacology of Inhalation Anesthetics. In: Anesthesia and anelgesia in laboratory animals. Academic Press, San Diego, California: pp 29-41.
Bush BM (1991) Part 1 haematology. In: Interpretation of laboratory results for small animal clinicians. Oxford Blackwell Scientific publication: pp 31-219.
Clayton JA, Collins FS (2014) Policy: NIH to balance sex in cell and animal studies. Nature 509:282-283.
Deckardt K, Weber I, Kaspers U, Hellwig J, Tennekes H, van Ravenzwaay B (2007) The effects of inhalation anaesthetics on common clinical pathology parameters in laboratory rats. Food Chem Toxicol 45:1709-1718.
Fish RE (1997) Pharmacology of Injectable Anesthetics. In: Anesthesia and analgesia in laboratory animals. Academic Press, San Diego, California: pp 1-28.
Gärtner K, Büttner D, Döhler K, Friedel R, Lindena J, Trautschold I (1980) Stress response of rats to handling and experimental procedures. Lab Anim. 14:267-74.
Gonullu E, Ozkardesler S, Kume T, Duru LS, Akan M, Guneli ME, Ergur BU, Meseri R, Dora O (2014) Comparison of the effects of dexmedetomidine administered at two different times on renal ischemia/reperfusion injury in rats. Braz J Anesthesiol 64:152-8.
Gothelf A, Hojman P, Gehl J (2009) Change in Hemoglobin Levels due to Anesthesia in Mice: An Important Confounder in Studies on Hematopoietic Drugs. Biol Proced Online 11:325-330.
Kharasch ED, Frink EJ Jr, Zager R, Bowdle TA, Artru A, Nogami WM (1997) Assessment of low-flow sevoflurane and isoflurane effects on renal function using sensitive markers of tubular toxicity. Anesthesiology 86:1238-1253.
Levine BS. (1995). Animal Clinical Pathology. In CRC Handbook of Toxicology. Boca Raton, FL: CRC Press pp 517-537.
Lugo-Roman LA, Rico PJ, Sturdivant R, Burks R, Settle TL (2009) Effects of serial anesthesia using ketamine or ketamine/medetomidine on hematology and serum biochemistry values in rhesus macaques ( Macaca Mulatta). J Med Primatol 39:41-49.
Marini RP, Jackson LR, Esteves MI, Andrutis KA, Goslant CM, Fox JG (1994) Effect of isoflurane on hematologic variables in ferfor rets. Am J Vet Res 55:1479-83.
Md Said S, Abiola O (2014) Haematological profile shows that Inbred Sprague Dawley rats have exceptional promise for use in biomedical and pharmacological studies. Asian Journal of Biomedical and Pharmaceutical Sciences 4:33-37.
Nishiyama T (2013) Effects of repeat exposure to inhalation anesthetics on liver and renal function. J Anaesthesiol Clin Pharmacol 29:83-87.
Nishiyama T, Yokoyama T, Hanaoka K (1998) Liver and renal function after repeated sevoflurane or isoflurane anaesthesia. Can J Anaesth 45:789-793.
Prendergast BJ, Onishi KG, Zucker I (2014) Female mice liberated for inclusion in neuroscience and biomedical research. Neurosci Biobehav Rev 40:1-5.
Risling TE, Caulkett NA, Florence D (2012) Open-drop anesthesia for small laboratory animals. Can Vet J 53:299-302.
Steffey EP, Gillespie JR., Berry JD, Eger EI, Schalm OW (1976) Effect of halothane and halothane-nitrous oxide on haematocrit and plasma protein concentration in dogs and monkeys. Am J Vet Res 37:959-62.
Tabata H, Kitamura T, Nagamatsu N (1998) Comparison of effects of restraint, cage transportation, anaesthesia and repeated bleeding on plasma glucose levels between mice and rats. Lab Anim 32:143-148.
Tanaka K, Kawano T, Tomino T, Kawano H, Okada T, Oshita S, Takahashi A, Nakaya Y (2009) Mechanisms of impaired glucose tolerance and insulin secretion during isoflurane anesthesia. Anesthesiology 111:1044-1051.
Tanaka T, Nabatame H, Tanifuji Y (2005) Insulin secretion and glucose utilization are impaired under general anesthesia with sevoflurane as well as isoflurane in a concentration-independent manner. J Anesth 19:277-281.
Taylor DK, Mook DM (2009) Isoflurane Waste Anesthetic Gas Concentrations Associated with the Open-Drop Method. J Am Assoc Lab Anim Sci 48:61-64.
Walter GL, Smith GS, Walker RM (2013) Interpretation of Clinical Pathology Results in Non-Clinical Toxicology Testing. In: Haschek and Rousseaux’s Handbook of Toxicologic Pathology. 3rd ed, 2nd vol, Elsevier, Amsterdam: pp 853-892.
Wilson DV, Evans AT, Carpenter RA, Mullineaux DR (2004) The effect of four anesthetic protocols on splenic size in dogs. Vet Anaesth Analg. 31:102-108.
Wixson SK, Smiller KL (1997) Anesthesia and analgesia in rodents. In: Anesthesia and analgesia in laboratory animals. Academic Press, San Diego, California: pp 165-204.
Zuurbier, CJ, Keijzers PJ, Koeman A, Van Wezel HB, Hollmann MW (2008) Anesthesia’s effects on plasma glucose and insulin and cardiac hexokinase at similar hemodynamics and without major surgical stress in fed rats. Anesth Analg 106:135-142.