Remarkable characterization and determination of atypical E. coli O157 none producing shiga toxin which can produce cytopathic effect on Vero cell and diarrhea in mice

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DOI: https://doi.org/10.12681/jhvms.20814
M. MOSHIRI
Bacteriology Department, Azad University of Jahrom
Y. TAHAMTAN
Microbiology Department, Shiraz Branch, Razi Vaccine and Serum Research Institute, Agriculture Research, Education and Extension Organization (AREEO)
M. NAMAVARI
Cellular Biology Departments, Shiraz Branch, Razi Vaccine and Serum Research Institute, Agriculture Research, Education and Extension Organization (AREEO)
Abstract

This study focused on the cytopathic effect of an unusual form of E. coli O157:H7 (atypical E. coli O157 (a-O157)) on Vero cell and infant mice. Multiplex PCR assay showed that they did not carry the gene for either shiga toxin 1 (stx1) or stx2 and other virulence factors. Increased changes in epithelial cell morphology, inter and intracellular gap junction and invading were assessed using Vero assay. In addition, a-O157infection causes disruptions of intercellular tight junctions, leading to clinical sequelae that include acute diarrhea in mice. Vero cell monolayers were exposed to a-O157influx and disintegrated tight junction of Vero cell line. Infected Vero cell enhance the number of attaching and effacing (A/E) lesions. Collectively, these findings provide in vitro evidence that Vero cell infected by a-O157was shown injury in epithelial cell barrier and induced A/E lesion the same as E. coli O157:H7 stx positive. These variants cannot be diagnosed by routine monitoring methods like biochemical assay for E. coli O157:H7 stx positive control. Finally, the data suggest that not only typical but also a-O157are an important cause of diarrhea in mice and produce cytopathic effect on Vero cell and may be harmful for food animal and also human.

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