Molecular Identification of Vancomycin Resistance and Virulence Genes in Foodborne Enterococci

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DOI: https://doi.org/10.12681/jhvms.20819
T. E. MUS
Department of Food Processing, Vocational School of Keles, Bursa Uludag University
F. CETINKAYA
Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Bursa Uludag University
R. CIBIK
Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Bursa Uludag University
G. DEGIRMENCI
Ministry of Food, Agriculture and Livestock, Ovacık District Directorate
F. B. DILER
Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Bursa Uludag University
Abstract

The study was performed to determine the presence of vancomycin phenotyping genes and some virulence traits in enterococci species. For this purpose, a total of 42 enterococci including 6 vancomycin-resistant and 36 vancomycin-susceptible strains originated from meat/meat products and milk/dairy products were assessed for the vanA, vanB and vanC genes and agg, esp, gelE, ace and efaA virulence genes by using polymerase chain reaction or multiplex polymerase chain reaction. The vanA gene was found in 12% (n=5) of the strains and vanC gene in 50% (n=21). From these, three vanA- (E. faecalis, E. durans, E. casseliflavus) and two vanC-positive (E. durans) strains had a minimum inhibitory concentration of > 256 μg/ml as previously determined with the E-test. The strains expressing vancomycin susceptibility originating from ready-to-eat food were found to carry vanA (n=1) and vanC (n=5) genes. On the other hand, the vanB gene was not detected among strains. Moreover, no strain was found to harbor virulence traits studied. Our results indicated that resistant or susceptible enterococci from foods of animal origin can be a possible reservoir for resistance genes and may have a potential role for transfer of genetic elements among enterococci or to other bacteria. Furthermore, to develop epidemiological surveillance systems for foodborne antibiotic resistant pathogens as vancomycin-resistant enterococci and their genes responsible for resistance, primarily vanA, vanB, continues to be an essential issue all around the world. The present work provides data for foodborne enterococci isolates harboring vanA gene from Turkey.

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Literaturhinweise
Biswas PP, Dey S, Sen A, Adhikari L (2016) Molecular characterization of virulence genes in vancomycin-resistant and vancomycin-sensitive enterococci. J Global Infect Dis 8:16-24.
Cattoir L, Leclercq R (2013) Twenty-five years of shared life with vancomycin-resistant enterococci: is it time to divorce? J Antimicrob Chemother 68:731-742.
Cetinkaya F, Elal Mus T, Soyutemiz GE, Çıbık R (2013) Prevalance and antibiotic resistance of vancomycin-resistant enterococci in animal originated foods. T J Vet Anim Sci 37:588-593.
Cetinkaya Y, Falk P, Mayhall CG (2000) Vancomycin-Resistant Enterococci. Clin Microbiol Rev 13:686-707.
Chajecka-Wierzchowska W, Zadernowska A, Łaniewska-Trokenheim L (2017) Virulence factors of Enterococcus spp. presented in food. LWT-Food Sci Technol 75:670-676.
Chajecka-Wierzchowska W, Zadernowska A, Łaniewska-Trokenheim L (2016) Diversity of antibiotic resistance genes in Enterococcus strains isolated from ready-to-eat meat products. J Food Sci 81:2799-2807.
D’Costa VM, King CE, Kalan L, Morar M, Sung WWL, Schwarz C, Froese D, Zazula G, Calmels F, Debruyne R, Golding GB, Poinar HN, Wright GD (2011) Antibiotic resistance is ancient. Nature 477:457-61.
De Moura TM, Cassenego APV, Campos FS, Ribeiro AML, Franco AC, d’ Azevedo PA, Frazzon J, Frazzon APG (2013) Detection of vanC1 gene transcription in vancomycin-susceptible Enterococcus faecalis. Mem Inst Oswaldo Cruz 108:453-456.
Diarra MS, Rempel H, Champagne J, Masson L, Pritchard J, Topp E (2010) Distribution of antimicrobial resistance and virulence genes in Enterococcus spp. and characterization of isolates from broiler chickens. Appl Environ Microbiol 76:8033-8043.
Dutka-Malen S, Evers S, Courvalin P (1995) Detection of glycopeptides resistance genotypes and identification to the species level of clinicallyrelevant enterococci by PCR. J Clin Microbiol 33:24-27.
Eaton TJ, Gasson MJ (2001) Molecular screening of Enterococcus virulencedeterminants and potential for genetic exchange between food and medical isolates. Appl Environ Microb 67:1628-1635.
Evers S, Sahm DF, Courvalin P (1993) The vanB gene of vancomycin-resistant Enterococcus faecalis V583 is structurally related to genes encoding D-Ala:D-Ala ligases and glycopeptide-resistance proteins vanA and vanC. Gene 124:143-144.
Farahani A (2016) State of Globe: Enterococci: Virulence Factors and Biofilm Formation. J Glob Infect Dis 8:1-2.
Gaglio R, Couto N, Marques C, Lopes MFS, Moschetti G, Pomba C, Settanni L (2016) Evaluation of antimicrobial resistance and virulence of enterococci from equipment surfaces, raw materials, and traditional cheeses. Int J Food Microbiol 236:107-114.
Gousia P, Economou V, Bozidis P, Papadopoulou C (2015) Vancomycin-resistance phenotypes, vancomycin-resistance genes, and resistance to antibiotics of enterococci isolated from food of animal origin. Foodborne Pathog Dis 12:214-220.
Hammad AM, Hassan HA, Shimamoto T (2015) Prevalence, antibiotic resistance and virulence of Enterococcus spp. in Egyptian fresh raw milk cheese. Food Control 50:815-820.
Handwerger S, Perlman DC, Altarac D, McAuliffe V (1992) Concomitant high level vancomycin and penicillin resistance in clinical isolates of enterococci. Clin Infect Dis 14:655-661.
Harada T, Kawahara R, Kanki M, Taguchi M, Kumeda Y (2012) Isolation and characterization of vanA genotype vancomycin-resistant Enterococcus cecorum from retail poultry in Japan. Int J Food Microbiol 153:372-377.
Jackson CR, Fedorka-Cray PJ, Barrett JB (2004) Use of genus and species spesific multiplex PCR for identification of enterococci. J Clin Microbiol 42:3558-3565.
Kasımoglu-Dogru A, Gencay YE, Denız Ayaz N (2010) Prevalence and antibiotic resistance profiles of Enterococcus species in chicken at slaughter level; absence of vanA and vanB genes in E. faecalis and E. faecium. Res Vet Sci 89:153-158.
Klibi N, Ben Said L, Jouini A, Slama KB, Lopez M, Sallem RB, Boudabous A, Torres C (2013) Species distribution, antibiotic resistance and virulence traits in enterococci from meat in Tunisia. Meat Sci 93:675-680.
Lefort A, Arthur M, Depardieu F, Chau F, Pouzet C, Courvalin P, Fantin B (2004) Expression of glycopeptide-resistance gene in response to vancomycin and teicoplanin in the cardiac vegetations of rabbits infected with vanB-type Enterococcus faecalis. J Infect Dis 189: 90-97.
Lopez M, Saenz Y, Rojo-Bezares B, Martinez S, del Campo R, Ruiz-Larrea F, Zarazaga M, Torres C (2009) Detection of vanA and vanB2-containing enterococci from food samples in Spain, including Enterococcus faecium strains of CC17 and the new singleton ST425. Int J Food Microbiol 133:172-178.
Mannu L, Paba A, Daga E, Comunian R, Zannetti S, Dupre I, Sechi LA (2003) Comparison of the incidence of virulence determinants and antibiotic resistance between Enterococcus faecium strains of dairy, animal and clinical origin. Int J Food Microbiol 88:291-304.
Osman KM, Ali MN, Radwan I, ElHofy F, Abed AH, Orabi A, Fawzy NM (2016) Dispersion of the vancomycin resistance genes vanA and vanC of Enterococcus isolated from Nile Tilapia on retail sale: A public health hazard. Front Microbiol 7:1354.
Oravcova V, Hadelova D, Literak I (2016) Vancomycin-resistant Enterococcus faecium with vanA gene isolated for the first time from wildlife in Slovakia. Vet Microbiol 194:43-47.
Perin LM, Miranda RO, Todorov SD, Franco BDGM, Nero LA (2014) Virulence, antibiotic resistance and biogenic amines of bacteriocinogenic lactococci and enterococci isolated from goat milk. Int J Food Microbiol 185:121-126.
Shankar V, Baghdayan A, Huycke MM, Lindahl G, Gilmore MS (1999) Infection-derived Enterococcus faecalis strains are enriched in esp, a gene encoding a novel surface protein. Infect Immun 67:193-200.
Toğay SO, Keskin AÇ, Açık L, Temiz A (2010) Virulence genes, antibiotic resistance and plasmid profiles of Enterococcus faecalis and Enterococcus faecium from naturally fermented Turkish foods. J Appl Microbiol 109:1084-1092.
Werner G (2012) Current trends of emergence and spread of vancomycin-resistant enterococci. In: Antibiotic Resistant Bacteria - A ContinuousChallenge in the New Millennium. InTech, Croatia: pp 303-354.
Yilmaz ES, Aslantas O, Pehlivanlar Onen S, Turkyilmaz S, Kurekci C (2016) Prevalence, antimicrobial resistance and virulence traits in enterococci from food of animal origin in Turkey. LWT-Food Sci Technol 66:20-26.