Molecular typing of Staphylococcus aureus isolated from bovine and poultry based on PCR-RFLP of spa gene
Abstract
Staphylococcus aureus (S. aureus) is considered one of the most important pathogen in bovine and poultry. The aim of the present study was molecular typing of S. aureus strains isolated from bovine mastitis and poultry by PCR-RFLP of spa gene. Fifty eight isolates of staphylococcus aureus were obtained from bovine mastitis (n=30) and poultry (n=28). Isolation identification were done based on conventional methods then confirmed by PCR method. The spa typing was performed by amplification of the spa gene X region. Bsp143I enzyme have been used for digestion of the PCR products and analyzed by electrophoresis on 1.5% gel. Gel Compare II software was used in pattern analysis and isolates with ≥ 70 percent similarities were taken as different types. Dendrogram obtained by RAPD results showed that among the 58 S. aureus strains eight RAPD types were identified (A–H). RAPD type A was more common among isolates from bovine mastitis (11 of 30 samples) and the majority (90%) of bovine isolates clustered in group A, D and E. Cluster B contains most S. aureus isolates of poultry origin. Group B, C and G clustered 82.1% of poultry host isolates, only in two clusters (F and H), some isolates from both the bovine and poultry host clustered together (7/58, 12%). In conclusion, RFLP of spa gene product using Bsp143I enzyme can be applied perfectly for differentiating and typing of S. aureus isolates were recovered from bovine mastitis and poultry infections.
Article Details
- Zitationsvorschlag
-
Khoramian, B., & Razmyar, J. (2023). Molecular typing of Staphylococcus aureus isolated from bovine and poultry based on PCR-RFLP of spa gene. Journal of the Hellenic Veterinary Medical Society, 74(1), 5095–5100. https://doi.org/10.12681/jhvms.22784 (Original work published 11. April 2023)
- Ausgabe
- Bd. 74 Nr. 1 (2023)
- Rubrik
- Research Articles
Dieses Werk steht unter der Lizenz Creative Commons Namensnennung - Nicht-kommerziell 4.0 International.
Authors who publish with this journal agree to the following terms:
· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
