Molecular identification of virulence features of Salmonella enterica isolated from fresh chicken meat sold at retail shops and markets in Peshawar, Pakistan

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Veröffentlicht: янв. 20, 2023
DOI: https://doi.org/10.12681/jhvms.27474
Shaista Rahat
Centre of Biotechnology and Microbiology, University of Peshawar, Pakistan
https://orcid.org/0000-0001-6637-2286
Kafeel Ahmad
Centre of Biotechnology and Microbiology, University of Peshawar, Pakistan
https://orcid.org/0000-0002-0532-0631
Amjad Ali
Centre of Biotechnology and Microbiology, University of Peshawar, Pakistan
Abstract

Salmonella enterica is an important agent of food borne illnesses in humans.  Poultry meat and products are easily contaminated with different strains of Salmonella enterica. Consumption of such food causes salmonellosis in human. The main aim of present investigation was to survey the prevalence of virulence associated genes of Salmonella enterica isolated from fresh chicken meat available in retail shops and markets at District Peshawar, Pakistan. Fresh chicken meat samples were collected from retail shops and markets at District Peshawar, Pakistan. The samples were processed for isolation of Salmonella enterica using selective media. Presumptive isolates were confirmed as Salmonella enterica via different biochemical tests and by amplification of invA gene. Isolates were tested for the presence of eight different virulence genes invA, spiA, cdtB, prgH, orgA, lpfC, sopB, and pefA. Amongst all isolates, 90(100%) had invA gene, 44(48.8%) had lfpC gene, 40(44.4%) had sopB gene, 27(30%) had orgA gene, 24(26.6%) hand spiA gene, 19(21.1%) had pefA gene, 18(20%) had prgH gene and 6(6.6%) had cdtB gene. 15.5% of the isolates had five virulence genes and 21.1% had four virulence genes. The findings of this study highlight an alarming public health risk and demand for strict bio-safety hygiene measures to be taken.

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Autor/innen-Biografie
Kafeel Ahmad, Centre of Biotechnology and Microbiology, University of Peshawar, Pakistan
http://www.uop.edu.pk/departments/Teaching-Faculty/?r=175&q=Dr-Kafeel-Ahmad
Literaturhinweise
Agron GP, Walker RL, Kind H, Sawyer SJ, Hayes DC, Wollard J, Andersen GL (2001) Identification by subtractive hybridization of sequences specific for Salmonella serovar Enteritidis. Appl Environ Microbiol 67(11): 4984-4991.
Amavisit P, Browing GF, Lightfood D, Anderson CS (2001) Rapid PCR detection of Salmonella in horse faecal samples. Vet Microbiol 79: 63-74.
Beceiro A, Tomás M, Bou G (2013) Antimicrobial resistance and virulence: successful or deleterious association in the bacterial world. Clin Microbiol Rev 26:185-230.
Borges KA, Furian TQ, Borsoi A, Moraes HL, Salle CT, Nascimento VP (2013) Detection of virulence-associated genes in Salmonella Enteritidis isolates from chicken in South of Brazil Pesqui Vet Bras 33:1416-22.
Borges KA, Furian TQ, Souza SN, Salle CT, Moraes HL Nascimento VP (2019) Antimicrobial resistance and molecular characterization of Salmonella enterica serotypes isolated from poultry sources in Brazil. Braz J Poult Sci 61:1-7.
Chiu CH, Ou JT (1996) Rapid identification of Salmonella serovars in feces by specific detection of virulence genes, invA and spvC, by an enrichment broth culture-multiplex PCR combination assay. J Clin Microbiol 34:2619-2622.
Choudhury M, Borah P, Sarma HK, Barkalita LM, Deka NK, Hussain I, Hussain MI (2016) Multiplex-PCR assay for detection of some major virulence genes of Salmonella enterica serovars from diverse sources. Curr Sci 111: 1252-1258.
Chuanchuen R, Sirintip K, Pawin P (2007) Occurrence of qacE/qacEΔ1genes andtheir correlation with class 1 integrons in Salmonella enterica isolates from poultry and swine. Southeast Asian J trop med public health 38:855-62.
Dione MM, Ikumapayi U, Saha D, Mohammed NI, Adegbola RA, Geerts S, Ieven M, Antonio M (2011) Antimicrobial resistance and virulence genes of non-typhoidal Salmonella isolates in The Gambia and Senegal. J Infect Dev Ctries, 5(11): 765-775.
El-Tawab A, Ashraf A, Nabih AM, Agag MA, Ali A, Marwah H (2017) Molecular studies of virulence genes of Salmonella Typhimurium causing clinical mastitis in dairy cattle. Benha Vet Med J 33(2): 27-37.
Huehn S, Ragione RML, Anjum M, Saunders M, Woodward MJ, Bunge C, Helmuth R, Hauser E, Guerra B, Beutlich J, Brisabois A (2010) Virulotyping and antimicrobial resistance typing of Salmonella enterica serovars relevant to human health in Europe. Foodborne Pathog Dis 7: 523-535.
Kim JE, Lee Y (2017) Molecular characterization of antimicrobial resistant non-typhoidal Salmonella from poultry industries in Korea. Irish Vet J 70(1):1-9.
Kubori T, Matsushima Y, Nakamura D, Uralil J, Lara-Tejero M, Sukhan A, Galán JE, Aizawa SI (1998) Supramolecular structure of the Salmonella typhimurium type III protein secretion system. Science 280(5363): 602-605.
Latasa C, García B, Echeverz M, Toledo-Arana A, Valle J, Campoy S, García-del Portillo F, Solano C, Lasa I (2012) Salmonella biofilm development depends on the phosphorylation status of RcsB. J bacteriol 194(14): 3708-3722.
Lekshmi M, Ammini P, Kumar S, Varela MF (2017) The food production environment and the development of antimicrobial resistance in human pathogens of animal origin. Microorganisms 5(1):11.
Miranda JM, Vazquez BI, Fente CA, Calo-Mata P, Cepeda A, Franco CM (2008) Comparison of antimicrobial resistance in Escherichia coli, Staphylococcus aureus, and Listeria monocytogenes strains isolated from organic and conventional poultry meat. J food prot 71(12): 2537-2542.
Murugkar HV, Rahman H, Dutta PK (2003) Distribution of virulence genes in Salmonella serovars isolated from man & animals. Indian J Med Res 117: 66–70.
Nayak R, Stewart T, Wang RF, Lin J, Cerniglia CE, Kenney PB (2004) Genetic diversity and virulence gene determinants of antibiotic-resistance Salmonella isolated from preharvest turkey production sources. Int J Food Microbiol 91: 51-62.
Nikaido H (2003) Molecular basis of bacterial outer membrane permeability revisited. Microbiol Mol Biol Rev 67:593-656.
Oliveira SDD, Rodenbusch CR, Michael GB, Cardoso MI, Canal CW, Brandelli A (2003) Detection of virulence genes in Salmonella Enteritidis isolated from different sources. Braz J Microbiol 34: 123-124.
Piccini G, Montomoli E (2020) Pathogenic signature of invasive non-typhoidal Salmonella in Africa: implications for vaccine development. Hum Vaccin Immunother, 16(9): 2056-2071.
Schechter LM, Lee CA (2000) Salmonella invasion of non-phagocytic cells. Subcell Biochem 33:289-320.
Skyberg JA, Logue CM, Nolan LK (2006) Virulence genotyping of Salmonella spp with multiplex PCR. Avian Dis 50: 77-81.
Smith JL Bayles DO (2006) The contribution of cytolethal distending toxin to bacterial pathogenesis Crit Rev Microbiol 32: 227-248.
Swamy SC, Barnhart HM, Lee MD, Dreesen DW (1996) Virulence determinants invA and spvC in Salmonellae isolated from poultry products, wastewater, and human sources. Appl Environ Microbiol 62: 3768-3771.
Tatavarthy A, Cannons A (2010) Real-time PCR detection of Salmonella species using a novel target: the outer membrane porin F gene (ompF). Lett Appl Microbiol 50:645-52.
Uchiya K, Nikai T (2008) Salmonella virulence factor SpiC is involved in expression of flagellin protein and mediates activation of the signal transduction pathways in macrophages. Microbiology 154: 349-3502.
Zou W, Al-Khaldi SF, Branham WS, Han T, Fuscoe JC, Han J, Foley SL, Xu J, Fang H, Cerniglia CE, Nayak R (2011) Microarray analysis of virulence gene profiles in Salmonella serovars from food/food animal environment. J Infect Dev Ctries 5(2): 94-105.