The relationships between Pit-1 gene polymorphism and some performance traits in simmental and brown swiss breeds


O Toyğar
https://orcid.org/0000-0001-6619-7342
M Ozdemir
https://orcid.org/0000-0002-1301-0270
Abstract

It was aimed to investigate the genotypic structures of the Pit-1 gene from genomic DNA samples taken from Simmental and Brown Swiss breeds and determine the distribution of genotype and allele frequencies of bovine breeds and to determine the relationships between the detected genotypes and some performance traits. Pit-1/HinfI gene polymorphisms were determined using the PCR-RFLP method from genomic DNA obtained from Brown Swiss and Simmental breed cattle used in the study. According to the Hardy-Weinberg genetic test, the distribution of genotype frequencies of the studied population was found to be in equilibrium (p˃0.05) in both breeds. Genotype frequencies of AA, AB, and BB alleles of the Pit-1 gene were determined as 5.73%, 36.42%, and 57.85%, respectively, for Simmental breed, while the genotype frequencies for Brown Swiss breed were determined as 9.43%, 42.86%, and 48.01%, respectively. The actual milk yield for Simmental breed was determined as 5953 ± 364.2, 5212 ± 993.4, 5507 ± 889.5 kg, the 305-day milk yield as 5953 ± 276.8, 5642 ± 782.7, 5427 ± 246.7 kg, the lactation period as 307 ± 47, 293 ± 29, 303 ± 22 days, the daily milk yield as 19.53 ± 6, 18.49 ± 8, 17.81 ± 2 kg for AA, AB, and BB genotypes, respectively. The actual milk yield for the Brown Swiss breed was determined as 3606 ± 253, 3558 ± 530, 3999 ± 099 kg, the 305-day milk yield as 4920 ± 217, 4462 ± 900, 4635 ± 870 kg, the lactation period as 222 ± 36, 240 ± 04, 258 ± 37 days, the daily milk yield as 17.62 ± 2, 15.49 ± 2, 16.04 ± 3 kg for AA, AB, and BB genotypes, respectively. In the statistical analyses, the relationships between the performance traits studied and Pit-1 genotypes in Simmental and Brown Swiss cattle were not prove to be significant statistically.

Article Details
  • Rubrik
  • Research Articles
Downloads
Literaturhinweise
Arnim Y, Afrani T, Putra DE (2017) Short Communication: Assocation of GH, IGF1R1, and PIT1 genes polymorphısm with average Daily gain and body measurment in Pesisir cattle.Depertment of Animal Production Faculty of A nimal Science Universites Andalas Padang Nusantra Bioscience Vol.10 No.4, Sumatra, Indonesia
Aytekin I, Boztepe S (2013) Associations of Pit-1 gene polymorphism with milk yield and composition traits in brown swiss cattle The Journal of Animal & Plant Sciences, 23(5): 1281-1289.
Bayram D, Arslan K, Akyüz B, İşcan KM (2017) Identification of pituitary-specific transcription factor-1 (PIT-1) and leptin gene (LEP) polymorphism of Holstein cattle reared in Turkey ,Ankara Üniv Vet Fak Derg, 64, 337-343.
Beigi Nassiri MT., Briandvand Z, Hartatik T, Fayazail J,Tavakoli S (2010) The study of of Pit-1 gene polymorphısmın Najdı cattle usıng PCR-RFLP method Asian J. Biol. Chem. 269 (47).
Dinçel D (2018) Genetıc polymorphısm of PROP1 gene ın Karacabey Merıno sheep Uludağ University.
Dybus A, Zatkowska I, Czerniawska-Piatkowska E, Grzesiak W, Wojcik J, Edyta R, Zych S (2004) PIT1-HinfI gene polymorphism and its associations with milk production traits in polish Black-and-White cattle Arch. Tierz., Dummerstorf 47(6):557-563.
Groza I, Pamfil D, Cenarui M, Cherey R (2005) Genetic markers in dairy cattle milk production unıversity of agrıculture scıences and veterinary medicine Cluj-Romanıa 62:2005.
Harvey C (1990) The Econometric Anlaysis of time Sciences Books Cambridge ,Massachusetts, The MIT T he second London Scoohl of Econumics Pres Second Edition.
Heidari M, Azari MA, Hasani S, Khanahmadi A, Zerehdaran S (2012) Effect of polymorphic variants of GH, Pit-1, and β-LG genes on milk production of Holstein cows Russian Journal of Genetics volume 48: 417–421.
Hoseinzadeh ZE, Mohammadabadi MR, Esmailizadeh AK, Khezri A (2015) Associations of Pit-1 Gene and Milk Protein Percentage in Holstein Cattle. J. Livestock Sci. Tech.
Hussain AD (2016) Genetic Structure analysis of Pıt-1/HınfI Gene and İts Relationships with someone Productıve Traits in Iraq and Hybrid Cows Populatons Instıtu Of Genetic Engineering and Biyotecnology Unıversity of Baghdad/Iraq 5(7): 226-235.
Lin Jia-peng, San-gang HE, Jie BAI, Lei LIU, Bo YANG, Li-xin TAN, Ming-Jun LIU (2009) Correlations between POU1F1 Polymorphism and Milk Yield in Xinjiang Brown Breed and Chinese Holstein.The Key Lab.of Livestock Reproduction & Breed Biotechnology of MOA,Xinjiang Academy of Biotechnological Center,Xinjiang Academy of Animal Science,China.
Moddy D., Pomp D, Brande E (1995) Restriction fragment lenght polymorphısm in amplification products o the bovine Pit-1 gene and assigment of Piı-1 to Bovine choromosome 1 depertmant of animal science unıversity Oklohoma State Unıversity feb:26(1):45-7.
Moddy D, Pomp D, Newman S, MacNeil MD (1996) Characterization of DNA Polymorphsıms ın three Populations of Hereford Cattle and Their Associations with Growth and Maternal EDP in Live Herefords , J. Animal Science 74:1784-1793.
Mukesh M, Sodhi M, Sobti RC, Parakash B, Kaushki R, Mishra BP (2008) Analaysıs of bovıne pıtuıtary spesıfıc transcrıptıon Factor Pit-1Hınf1 gene polymorphısm ın Indıan Zebuine cattle lıvestock Sci.113:81-86.
Özdemir M (2012) Determination of Pit-1/Hinf1 polymorphism in Holstein and Native Ear cattle raised as genetic resource in Turkey The Journal of Animal & Plant Sciences, 22(1): 2012, Page: 25-28 ISSN: 1018-7081.
Özdemir M, Topal M, Vecihi A (2018) The relationships between performance traits and the bGH/Alu I and Pit-1/Hinf I polymorphisms in Holstein cows Indian Journal of Animal Research: 2018.
Özek K (2015) Süt Sığırlarında Süt Kompozisyonunu Etkileyen Faktörler ve Beslenme-Süt Kompozisyonu İlişkisi ,Bahri Dağdaş Hayvancılık Araştırma Dergisi 37-45.
Özhan M, Tüzemen N, Yanar M (2012) Büyükbaş Hayvan Yetiştirme , Atatürk Üniversitesi Ziraat Fakültesi Ofset Tesisi , yayın no:134. Baskı ,Erzurum.
Pozovnikovaa MV, Rotarb LN, Kudinova AA, Dementievaa NV, Sabrier ML, Perez C, Leheup B, Copin B, Sizova D, Penzo MA, Stanger PZ, Cooke NE, Amselmes S (2020) The Linkage of Polymorphic Variants of Genes Gh, Prl, and Pit-1 and Milk Productivity of Cows with Morphology of Cumulus-Oocyte Complex Sampled Post Mortem 2016. Functional Characterization of Human POUF1 Mutation Associated with İsolated Growth Hormone Deficienciy: Anovel Etiology for IGHD . Hum. Mol genetic.
Renaville R, Gengler E, Vrech A, Prandi S, Massart S, Corradini C, Bertozzi CF, Mortiaux Burny P, (1997) Pit-1 Gene Polymorphism, Milk Yield, and Conformation Traits for Italian Holstein-Friesian Bulls Author links open overlay panel. Journal of Dairy Science. 80:(12): 3431-3438.
Scully KM, Michael G (2000) Rosenfeld Pituitary Development: Regulatory Codes in Mammalian Organogenesis Science, Vol. 295, Issue 5563, pp. 2231-2235 DOI: 10.112.
Selvaggi M, Dario C (2011) Analysis of two Pit-1 gene polymorphisms: Single nucleotide polymorphisms (SNPs) distribution patterns in Podolica cattle breed ,10-31 Issue Vol. 10 No. 55.
Sobrier M, Tsai C, Perez C, Leheup B, Bouceba T, Copin B, Sizova D, Penzo A, Stanger B, Cooke N, Amselem S (2016) Functıonal characterızatıon of human POU1F1 mutatıon assocıated wıth ısolated growth hormon edıffıcıency a novel etıology for IGHD :1;25(3):47283.
Tang L (2012) Association of polymorphisms in the Pit-1 intron 5 with body measurements in Chinese Cattle. African J. Biotechnology, 11:42. (doi:10.5897/AJB11.3737)
Trakovická A, Moravčíková N, Gábor M, Miluchová M (2014) Genetic polymorphism of Pit-1 gene associated with milk production traits in Holstein cattle Acta Agraria Kaposváriensis (2014) Vol 18 Supplement 1, 146-151. Kaposvári Egyetem, Agrár- és Környezettudományi Kar, Kaposvár Kaposvár University, Faculty of Agricultural and Environmental Sciences, Kaposvár.
Trakoviçka A, Moravcikova N, Minaroviç T, Navratilova T (2015) SNPs analyses of the bovine LEP and PIT-1 genes by multiplex PCR-RFLP method and their effect on milk performance traits in Slovak Simmental cattle 015, 16(1), p.65-75 65 Journal of Central European Agriculture, 2015, 16(1), p.65-75 65 Journal of Central European Agriculture, 2015, 16(1):65-75.
Woollard J, Tuggle CK, Ponce de leon FA (2000) Rapid Communication: Localization of POU1F1 to bovine ,ovine and caprine Iq21-22.J. Anim. Sci.78:242-242.
Viorica C (2006) RLFPHınfI Polymorphısms Between Exons 5 and Exon 6 of the Pıt-1 gene in Romanian Simmental Cattle USAMV Journal –CN 6326(219-222).
Vlaic A, Coiser V, Gaboreanu I (2007) Hinf1 Polymorphisms of K-Casein and Pit-1 genes in Romanian Simmental Cattle ,University of Agriculture Sciences and veterinary medicine Cluj-Romania vol (40).
Qiang Y, Zhoua F, Chuzhao L, Hong C, Xianyong L (2014) Relationship between genetic variants of POU1F1, PROP1, IGFBP3 genes and milk performance in Guanzhong dairy goats Small Ruminant Research Volume 140:40-45.
Yang D, Zhu F, Tang L, Guifang H, Chen H (2010) Relationship of Pit-1 polymorphisms with growth traits in Chinese Cattle 2010 International Conference on Biology, Environment and Chemistry IPCBEE vol.1 2011 IACSIT Press, Singapore.
Zabeel AK, Wefak G, Al-Bazil M, Muhammed HA (2018) Study the Effects of Pit-1 polymorphism on some blood Parameters in dairy cow in Karbala governorate Afrah Kamil Zabeel et al IOP Conf. Ser.: Mater. Sci. Eng. 571 012053.
Zhang C, Liu B, Hong C, Xianyong L, Chuzhao L, Zhiqing Z, Runfeng Z (2009) Associations of a HinfI PCR-RFLP of POU1F1 Gene with Growth Traits in Qinchuan Cattle Animal Biotechnology Volume 20 - Issue 2.
Zwierzchowski L, Kizyzeewski J, Strzalkowska N, Siadkowska E, Ryniewcz Z (2002) Effects of polymorphism of growth hormone (GH), Pit-1, and leptin (LEP) genes, cow's age, lactation stage and somatic cell count on milk yield and composition. Instittute of genetics and animal breeding, Poland. Animal science papers and reports, 20(4); 213-227
Am häufigsten gelesenen Artikel dieser/dieses Autor/in