Η ταυτοποίηση της Bartonella coopersplainsensis σε άγρια τρωκτικά (Rattus norvegicus) για πρώτη φορά στην Ελλάδα


Δημοσιευμένα: Nov 10, 2017
Λέξεις-κλειδιά:
Bartonella coopersplainsensis Rattus norvegicus Ελλάδα PCR
E. PAPADOGIANNAKIS (Ε. ΠΑΠΑΔΟΓΙΑΝΝΑΚΗΣ)
G. SPANAKOS (Γ. ΣΠΑΝΑΚΟΣ)
I. KONTOU (Ι. ΚΟΝΤΟΥ)
V. KONTOS (Β. ΚΟΝΤΟΣ)
E. VELONAKIS (Ε. ΒΕΛΟΝΑΚΗΣ)
D. SKLIVAS (Δ. ΣΚΛΗΒΑΣ)
C. KOUTIS (Χ. ΚΟΥΤΗΣ)
Περίληψη

Η μελέτη αυτή ήταν μια πρώτη προσπάθεια για ανίχνευση της Bartonella sp. σε ιστούς (ήπαρ και καρδιά) πουπάρθηκαν από άγρια τρωκτικά {Rattus norvegicus) στην κεντρική Ελλάδα. Εξετάστηκαν δεκαοκτώ (18) δείγματα ήπατος και δεκαοκτώ (18) δείγματα καρδιάς, τα οποία προέρχονταν από είκοσι τρία (23) ζώα. Δυο (2) δείγματα ήπατος βρέθηκαν θετικάενώ όλα τα δείγματα καρδιάς ήταν αρνητικά για το γονίδιο 16S-23S intergenic spacer rDNA της Bartonella sp. Η αλληλουχίατων βάσεων του DNA των θετικών δειγμάτων και η σύγκριση με τα διαθέσιμα δεδομένα της GenBank με τη χρησιμοποίησητου προγράμματος BLAST, αποκάλυψε το ίδιο είδος Bartonella και στα δύο θετικά δείγματα με 100% ομοιότητα με τηναλληλουχία των βάσεων της Bartonella coopersplainsensis.

 

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Αναφορές
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