Παρουσία και αντιμικροβιακή ευαισθησία των Enterococcus spp. στις έτοιμες για κατανάλωση σαλάτες, to περιβάλλον και to προσωπικό ενός εργοστασίου παραγωγής σαλατών στη Βόρεια Ελλάδα


Δημοσιευμένα: Μαρ 22, 2018
Λέξεις-κλειδιά:
Enterococcus spp. αντιμικροβιακή ευαισθησία έτοιμες για κατανάλωση σαλάτες περιβάλλον
D SERGELIDIS
A ABRAHIM
V ANAGNOSTOU
A PAPA
Th. PAPADOPOULOS
Περίληψη

Διακόσια εικοσιπέντε δείγματα από τις σαλάτες (τυροσαλάτα, ταραμοσαλάτα, μελιτζανοσαλάτα και τζατζίκι),το περιβάλλον και το προσωπικό ενός εργοστασίου παραγωγής σαλατών στην Βόρεια Ελλάδα εξετάστηκαν για την παρουσίατων Enterococccus spp. και την αντιμικροβιακή αντοχή τους. Απομονώθηκε Enterococcus faecium σε ποσοστό 12% τωνδειγμάτων. Ποσοστά 38,1% και 15% των δειγμάτων μελιτζανοσαλάτας και τυροσαλάτας, αντίστοιχα, ήταν θετικά. Τα ποσοστάαπομόνωσης από τα συστατικά των σαλατών ήταν 30% για τη μαγιονέζα, 20% για τη φέτα και 10% για τη μυζήθρα. Θετικάήταν, επίσης, 8,6% των δειγμάτων από το περιβάλλον, 20% από τις ποδιές, 20% από τα γάντια και 16,7% από τις ρινικέςκοιλότητες των εργαζομένων. Όλα τα απομονωθεντα στελέχη ήταν ευαίσθητα στα γλυκοπεπτίδια (βανκομυκίνη καιτεϊκοπλανίνη).Τα δυο απομονωθεντα στελέχη από τις ρινικές κοιλότητες των εργαζομένων ήταν ανθεκτικά στην πενικιλλίνη και τηναμπικιλλίνη, ενώ ένα από αυτά ήταν επιπλέον ανθεκτικό στη χλωραμφενικόλη. Όλα τα στελέχη που απομονώθηκαν από τοπεριβάλλον, τις σαλάτες και τα συστατικά τους ήταν ευαίσθητα στα τρία αυτά αντιβιοτικά, αλλά παρουσίασαν αντοχή σεπερισσότερες των τριών κατηγορίες αντιβιοτικών. Τα έτοιμα προς κατανάλωση τρόφιμα μπορεί να είναι δυνητικές πηγέςμόλυνσης του ανθρώπου με πολυναθεκτικοΰς στα αντιβιοτικά εντερόκοκους.

Λεπτομέρειες άρθρου
  • Ενότητα
  • Research Articles
Λήψεις
Τα δεδομένα λήψης δεν είναι ακόμη διαθέσιμα.
Αναφορές
Barbosa J, Ferreira V, Teixeira Ρ (2009) Antibiotic susceptibility of enterococcci isolated from traditional fermented meat products. Food Microbiol 26:527-532.
Bates J (1997) Epidemiology of vancomycin-resistant enterococci in the community and the relevance to farm animals to human infections. J Hospital Infection 37: 89-101.
Bager F, Madsen M, Christensen J, Aarestrup FM (1997) Avoparcin used as a growth promoter is associated with the occurrence of vancomycin resistant Enterococcus faecium on Danish poultry and pig farms. Prev Vet Med 31: 95-112.
Ben Omar N, Castro A, Lucas R, Abriouel H, Yousif NMK, Franz CMAP, Holzapfel, WH, Perez-Pulito R, MartineZ-Canamero M, Galvez A (2004) Functional and safety aspects of enterococci
isolated from different Spanish foods System. Appi Microbiol 27:118-130.
Boyle JM, Soumakis, SA, Rendo A, Herrington JA, Gianarakis DG, Thurberg BE, Painter BG (1993) Epidemiologic analysis and genotypic characterization of a nosocomial outbreak of vancomycin-resistant enterococci. J Clinical Microbiol 31:1280-1285.
Canzek Majhenij A, Rigelj I, Perko Β (2005) Enterococci from Tolmic cheese: population structure, antibiotic susceptibility and incidence of virulence determinants. J Food Microbiol 2:239-244.
Centeno JA, Mendez S, Podriguez-Otero JL (1996) Main microbial flora present as natural starters in Cebreiro raw cow's milk cheese (Northwest Spain). Int J Food Microbiol 33:307-313.
Chatterjee I, Iredell JR, Woods M, Lipmann J (2007) The implications of enterococci in the intensive care unit. Crit Care Resusc 9:69-75.
Chingwaru W, Mpuchane SF, Cashe Β A (2003) Enterococcus faecalis and Enterococcus faecium isolates from milk, beef and chicken and their antibiotic resistance. J Food Prot 66:931-936.
Citak S, Yucel N, Orhan S (2004) Antibiotic resistance and incidence of Enterococcus in Turkish white cheese. Int J Dairy Technol 57:27-31.
Clinical and Laboratory Standards Institute/CCLI (2009) Performance Standards for Antimicrobial Susceptibility Testing. Nineteenth informational supplement. M100-S19. CLSI, Wayne, PA.
Consentino S, Pisano MB, Corda A, Fadda ME, Piras C (2004) Genotypic and technological characterization of enterococci isolated from artisanal Fiore Sardo cheese. J Dairy Res 71:444-450.
Coppola S, Parente E, Dumonter S, La Peccerella A (1988) The microflora of natural whey cultures utilized as starter cultures in the manufacture of Mozzarella cheese from water-buffalo milk. Lait 68:295-310.
Franz CMAP, Holzapfel WH, Stiles ME (1999) Enterococci at the crossroad of food safety. Int J Food Microbiol 47:1-24.
Franz CMAP, Muscholl-Silberhorn AN, Yousif NMK, Vancanneyt M, Swings J, Holzapfel WH (2001) Incidence of virulence factors and antibiotic resistance among enterococci isolated from food. Appi Environ Microbiol 65:2385-4389.
Giraffa G, Olivari AM, Neviani E (2000) Isolation of vancomycin - resistant Enterococcus faecium from Italian cheese. Food Microbiol 17:671-677.
Godfree AF, Kay D, Wyer MD (1997) Faecal streptococci as indicators of faecal contamination in water. J Appi Microbiol Symposium Supplement 83:15-115.
Gomes BC, Esteves CT, Palazzo ICV, Darini ALC, Felis GÈ, Sechi LA, Franco BDGM, De Martini ECP (2008) Prevalence and characterization of Enterococcus spp. isolated from Brazilian foods. Food Microbiol 25:668-675.
Klein C, Pack A, Bonaparte C, Reuter G (1998) Taxonomy and physiology of probiotic lactic acid bacteria. Int J Food Microbiol 41:103-12.
Koluman A, Akan LS, Çakiroglu FP (2009) Occurrence and antimicrobial resistance of enterococci in retail foods. Food Control 20:281-283.
Litopoulou-Tzanetakis E, Tzanetakis N, Vafopoulou-Mastrojannaki A (1993) Effect and type of lactic starter on microbiological, chemical and sensory characteristics of feta cheese. Food Microbiol 10:31-41.
Lopes MF, Ribeiro T, Martins MP, Tenereiro R, Crespo MT (2003) Gentamicin resistance in dairy and clinical enterococcal isolates and in reference strains. J Antimicrob Chemother 52:214-219.
Lu HZ, Weng XH, Li H, Yin YK, Pang MY, Tang YW (2002) Enterococcus faecium-related outbreak with molecular evidence of transmission from pigs to humans. J Clinic Microbiol 40:913-917.
Majhenic AC, Rogelj I, Perko Β (2005) Enterococcci from Tolminc cheese: population, structure, antibiotic susceptibility and incidence of virulence determinants. Int J Food Micriobiol 102:239-244.
Maniatis AN, Pournaras S, Kanellopoulou M, Kontos F, Dimitroulia E, Papafrangas E, Tsakris A (2001) Dissemination of clonally unrelated erythromycin- and glycopeptide-resistant Enterococcus faecium isolates in a tertiary Greek hospital. J Clinic Microbiol 39:4571-4574.
Mannu L, Paba A, Daga E, Comunian R, Zanetti S, Dupre I, Sechi LA (2003) Comparison of the incidence of virulence determinants and antibiotic resistance between Enterococcus faecium strains of dairy, animal and clinical origin. J Food Microbiol 88:385-408.
Messi Ρ, Guerrieri E, de Niederhäusern S, Sabia C, Bondi M (2006) Vancomycin-resistant enterococcci (VRE) in meat and environmental samples. Int J Food Microbiol 107:218-222.
Metzidie E, Manolis EN, Pournaras W, Sofianou D, Tsakris A (2006) Spread of an unusual penicillin- and imipenem-resistant butampicillin-susceptible phenotype among Enterococcus faecalis clinical isolates. J Antimicrob Chemother 57:158-60.
Murray BE (1990) The life and times of the enterococci. Clinic Microbiol Rev 3:46-65.
Noble WG, Virani Z, Crée RGA (1992) Co-transfer of vancomycin and other resistance genes to Enterococcus faecalis NCTC 12201 to Staphylococcus aureus. FEMS Microbiology Letters 93:195-198.
Ogier JK, Serror Ρ (2008) Safety assessment of dairy microorganisms: The Enterococcus genus. J Food Microbiol 126:291-301.
Pavia M, Carmel G, Nobbles A, Splatter L, Angellala I (2000) Vancomycin resistance and antibiotic susceptibility of enterococci in raw meat. J Food Prot 63:912-915.
Peters J, Mac K, Wischmann-Schauber H, Klein G, Ellebroek L (2003) Species distribution and antibiotic resistance patterns of enterococci isolated from food of animal origin in Germany. J Food Microbiol 88:311-314.
Pournaras S, Malamou-Lada H, Maniati M, Mylona-Petropoulou D, Vagiakou-Voudris H, Tsakris A, Maniatis AN (2004) Persistence of a clone of glycopeptide-resistant Enterococcus faecalis among patients in an intensive care unit of a Greek hospital. J Antimicrob Chemother 53:109-12.
Quednau M, Ahmè S, Petersson AC, Molin G (1998) Antibiotic resistant strains of Enterococcus isolated from Swedish and Danish retailed chicken and pork. J Appi Microbiol 84:1163-1170.
Sabia C, de Niederhäusern S, Guerrieri E, Messi P, Anacarso I, Manicardi G, Bondi M (2008) Detection of becteriocin production and virulence traits in vancomycin-resistant enterococcci of different sources. J Appi Microbiol 104:970-979.
Sarantinopoulos Ρ, Kalantzopoulos G, Tsakalidou E (2002) Effect of Enterococcus faecium on microbiological, physicochemical and sensory characteristics of Greek feta cheese. Int J Food Microbiol 76:93-105.
Teuber M, Perreten V, Wirchin GF (1996) Antibiotikum resistente Bakterien: Eine neue Dimension in der Lebensmitteltechnologie. Lebensmittel-Technologie 29:182-189.
Teuber M, Meile L, Schwarz F (1999) Acquired antibiotic resistance in lactic acid bacteria from food. Antonie Leeuwenhoek 76:115-137.
Tzanetakis Ν, Litopoulou-Tzanetaki Ε (1992) Changes in numbers and kinds of lactic acid bacteria in Feta and Teleme, two Greek cheeses from ewe's milk. J Dairy Sci 75:1389-1393.
Weigel LM, Clewel DB, Gill SR, Clark NC, Mcdougal LK, Flannagan SE (2003) Genetic analysis of a high-level vancomycin resistant isolate of Staphylococcus aureus. Science 302:1569-1571.
Van den Braak N, Van Belkum A, Van Keulen M, Viegenthart J, Verbrugh HA, Endtz HP (1998) Molecular characterization of vancomycin-resistant enterococci from hospitalized patients and
poultry products in the Netherlands. J Clin Microbiol 36:1927-1932.
Wilson IG, Mcafee GG (2002) Vancomycin-resistant Enterococcci in shellfish, unchlorinated waters and chicken. Int J Food Microbiol 79:143-151.
Τα περισσότερο διαβασμένα άρθρα του ίδιου συγγραφέα(s)