Seroprevalence and risk factors assessment of Brucella spp. in dairy buffaloes in mainland Greece.


Published: Oct 26, 2022
Keywords:
brucellosis buffaloes seroprevalence risk factors Greece
A Papapostolou
EJ Petridou
https://orcid.org/0000-0002-3532-5724
K Papageorgiou
https://orcid.org/0000-0003-3341-6726
A Stournara
https://orcid.org/0000-0001-8054-4996
A Benos
https://orcid.org/0000-0002-5397-9385
S Lafi
https://orcid.org/0000-0002-7044-7428
N Giadinis
https://orcid.org/0000-0001-5646-3627
Abstract
Brucellosis is an infectious bacterial disease caused by members of genus Brucella, affecting both animals and humans, and resulting in both serious economic losses in animal production sector and deterioration of public health. A cross-sectional study was conducted from January to August of 2018 to determine the seroprevalence and associated risk factors of bovine brucellosis, in Central Macedonia and East Macedonia and Thrace, in mainland Greece. A total of 1,255 blood samples were collected using a simple random sampling technique from dairy buffaloes older than12 months. All serum samples were analyzed with Rose Bengal Test for screening and Complement Fixation Test for confirmation of the positive samples. Accordingly, the overall individual-level seroprevalence of dairy buffaloes in the study area was 0.72% (9/1,255, 95% CI:0.32-1.36%) while the overall herd-level seroprevalence was 15.38% (4/26, 95% CI:6.15-33.53%). Moreover, information was gathered on demographic characteristics of the owners, individual animals and herd level risk factors and other farm characteristics using a questionnaire. Higher prevalence of Brucella spp. was observed in buffaloes bred under intensive and semi-intensive feeding systems, compared to those bred under extensive ones. High seroprevalence of brucellosis was also observed in medium size herds. Two of the herds had recorded history of the disease, the last five years while, in these two herds delays in the implementation of brucellosis eradication programs were revealed. The present study indicated that the occurrence of brucellosis in Greece dairy buffalo farms is at a low magnitude. Even though the seroprevalence is low, it can still be potential hazard for both susceptible animals and humans
Article Details
  • Section
  • Research Articles
Downloads
Download data is not yet available.
References
Adesiyun A.A, Fosgate G.T., Persad A., Campbell M., Seebaransingh R. &Stewart-Johnson A. "Comparative study on responses of cattle and water buffalo (Bubalus bubalis) to experimental inoculation of Brucella abortus biovar 1 by the intraconjunctival route-a preliminary report." Trop. Anim. Health Prod., 2010: 1685-1694.doi: 10.1007/s11250-010-9621-3
Adesiyun A.A, Fosgate G.T., Seebaransingh R., Brown G., Stoute S. & Stewart-Johnson A. "Virulence of Brucella abortus isolated from cattle and water buffalo." Trop. Anim. Health Prod., 2011: 13-16.doi: 10.1007/s11250-010-9679-y
Al-MajaliA.M., A.M., Talafha, A.Q., Ababneh, M.M., Ababneh, M.M. "Seroprevalence and risk factors for bovine brucellosis in Jordan." Journal of Veterinary Science, 2009: 61–65.doi: 10.4142/jvs.2009.10.1.61
Amin M.R. Siddiki, M. A., Kabir, A. K. M. A., Faruque, M. O. and Khandaker, Z. H. "Status of buffalo farmers and buffaloes at Subornocharupozila of Noakhali district in Bangladesh." Progressive Agriculture, 2015: 71-78.doi:10.3329/pa.v26I1.24519
Aulakh HK., Patil PK, Sharma S, Kumar H, Mahajan V, Sandhu KS. " A study on the epidemiology of bovine brucellosis in Punjab (India) Using Milk-ELISA." Acta Vet Brno, 2008: 393–399.doi:10.2754/avb200877030393
Caporale V., Bonfini B., Di Giannatale et al., Di Provvido A., Forcella S., Giovannini A., Tittarelli M. &Scacchia M. "Efficacy of Brucella abortus vaccine strain RB51 compared to the reference vaccine Brucella abortus strain 19 in water buffalo." Vet. Ital, 2010: :13-19.
Coelho A., Ana Cláudia, Juan García Díez and Adosinda Maria Coelho. "Risk Factors for Brucella spp. in Domestic and Wild Animals." Chap. 1 in Updates on Brucellosis, by Ana Cláudia, Juan García Díez and Adosinda Maria Coelho Coelho et al, edited by Manal Mohammad Baddour. Alexandria: Alexandria University, 2015.doi:10.5772/61325
Cohen FB., Robins B, Lipstein W. "Isolation of Brucella abortus by percutaneous liver biopsy." N Engl J Med, 1957: 228 –230.doi: 10.1056/nejm195708012570508
Corbel M.J. "Brucellosis: an overview." Emerging Infectious Diseases, 1997 1997: 213– 221.doi: 10.3201/eid0302.970219
De Alencar Mota A.L.A., Ferreira F., Ferreira Neto J.S., Dias R.A., Amaku M., Hildebrand Grisi-Filho J.H. et al. 2016, ‘Large-scale study of herd-level risk factors for bovine brucellosis in Brazil’, Acta Tropica 164,(226–232). doi: 10.1016/j.actatropica.2016.09.016
Enright, F.M. The pathogenesis and pathobiology of Brucella infection in domestic animals. Florida: (K. Nielsen & R. Duncan, eds). CRC Press, Boca Raton, 1990.
Fero E., Arla Juma, Anita Koni, JonidaBoci, Toni Kirandjiski, Robert Connor, Gamal Wareth, Xhelil KoleciI. "The seroprevalence of brucellosis and molecular characterization of Brucella species circulating in the beef cattle herds in Albania." PLoS ONE, 2020. doi: 10.1371/journal.pone.0229741. eCollection 2020
.
Fosgate G.T., Adesiyun, A.A., Hird, D.W., Johnson, W.O., Hietala, S.K., Schrig, G.G., Ryan, J. "Comparison of serologic tests for detection of Brucella infections in cattle and water buffalo (Bubalus bubalis)." American Journal of Veterinary Research, 2002: 1598–1605.doi: 10.2460/ajvr.2002.63.1598
Foster G., Osterman BS, Godfroid J, Jacques I, Cloeckaert A. "Brucella ceti sp. nov. and Brucella pinnipedialis sp. nov. for Brucella strains with cetaceans and seals as their preferred hosts." Int J Syst Evol Microbio, 2007: 2688–2693.doi: 10.1099/ijs.0.65269-0
Godfroid J., Nielsen K, Saegerman C. "Diagnosis of brucellosis in livestock and wildlife." Croat Med J, 2010: 296–30.doi: 10.3325/cmj.2010.51.296
Godfroid J., Saegerman C, Wellemans V, Walravens K, Letesson JJ, Tibor A, McMillan A. "How to substantiate eradication of bovine brucellosis when specific serological reactions occur in the course of brucellosis testing." Vet Microbiol, 2002: 461–477.doi: 10.1016/s0378-1135(02)00230-4
Godfroid J., Scholz HC Barbier T. "Brucellosis at the animal/ecosystem/human interface at the beginning of the 21st century." Prev Vet Med, 2011: 118–131.doi: 10.1016/j.prevetmed.2011.04.007
Hadjichristodoulou Ch., Papatheodorou Ch, Soteriades E. "Epidemiological study of brucellosis in eight Greece villages using a computerized mapping program." European Journal of Epidemiology, 1999: 671-680.doi: 10.1023/a:1007673318947
Kansouzidou A., S. Mitka, BD. Danielides. Brucella species and biotypes isolated from humans. Acta Microbiologica Hellenica 1996, 41(6): 598-602
Kansouzidou, A. Ifantidou, S. Mitka, Ch. Charitidou, Th. Varnis, E. Chaidouli. Change in biotype of Brucella strains that cause disease in humans in Northern Greece. Acta Microbiologica Hellenica 2002, 47(5): 385-391
Katsiaounis Th., "Contribution to the study of the epidemiology of brucellosis search of microorganism in ruminants milk." Thessis, Aristotle University of Thessaloniki, 1996.
Klietmann., WF, Ruoff KL. "Bioterrorism: implications for the clinical microbiologist." Clin Microbiol Rev, 2001: 364-81.doi: 10.1128/CMR.14.2.364-381.2001
Konrad J.L., Campero L.M., Caspe G.S., Brithuega B., Draghi G., Moore D.P., Crudeli G.A., Venturini M.C. &Campero L.M. "Detection of antibodies against Brucella abortus, Leptospira spp., and Apicomplexa protozoa in water buffaloes in the Northeast of Argentina." Trop. Anim. Health Prod., 2013: 1751-1756.doi: 10.1007/s11250-013-0427-y
Ligda C. H. and Georgoudis, A. " Adaptation of buffalo production systems towards the market demand for certified quality products." Journal of Tekirdag Agricultural Faculty, 2005: 124-126.
Lytras T et al., Danis K, Dounias G. "Incidence patterns and occupational risk factors of human brucellosisinGreece,2004–2015"IntJ Occup Environ Med, 2016: 221-226.doi:10.15171/ijoem.2016.806
Mainar-Jaime Raúl C., Pilar M. Muñoz María J. de Miguel María J. Grilló. "Specificity dependence between serological tests for diagnosing bovine brucellosis in Brucella-free farms showing false positive serological reactions due to Yersinia enterocolitica O:9." Can Vet J, 2005: 913–916
Μantur BG.,Amarnath SK Shinde RS. "Review of clinical and laboratory features of human brucellosis." Indian J Med Microbiol, 2007: 188–202.doi: 10.4103/0255-0857.34758
Megersa B., Demelash Biffa, Fekadu Niguse,Tesfaye Rufael, Kassahun Asmare, and Eystein Skjerve. "Cattle brucellosis in traditional livestock husbandry practice in Southern and Eastern Ethiopia, and its zoonotic implication." Acta Vet Scand., 2011: 24.
Minas A., Minas, Μ., Stournara, A. and Tselepidis, S. "The "effects" of Rev-1 vaccination of sheep and goats on human brucellosis in Greece." Preven. Vet. Med, 2004: 41-47.doi:10.1016/j.prevetmed.2004.03.007
Mitka St., "Evaluation of the newest molecular techniques for the diagnosis of human brucellosis." Ph.D. thesis., Aristotle University of Thessaloniki, Greece, Thessaloniki, 2005.
Muma JB., Samui KL, Oloya J, Munyeme M, Skjerve E. "Risk factors for brucellosis in indigenous cattle reared in livestock-wildlife interface areas of Zambia." Preventive Veterinary Medicine., 2007: 306–317.doi: 10.1016/j.prevetmed.2007.03.003
OIE, 2018. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. Office Internationale Des Epizooties, Paris.
Pappas G.,Papadimitriou P, Akritidis N, Christou L, Tsianos EV. "The new global map of human brucellosis." Lancet Infect Disease, 2006: 91-9.doi: 10.1016/S1473-3099(06)70382-6
Poester F.P., L.E. Samartino & R.L. Santos. "Pathogenesis and pathobiology of brucellosis in livestock." Rev. sci. tech. Off. int. Epiz., 2013: 105-115.doi: 10.20506/rst.32.1.2193
Rhyan Jack C., Sam D. Holland, Thomas Gidlewski, Dennis A. Saari, Allen E. Jensen, Darla R. Ewalt, Steve G. Hennager, Steven C. Olsen, Norman F. Cheville. "Seminal vesiculitis and orchitis caused by Brucella abortus biovar 1 in young bison bulls from South Dakota." J Vet Diagn Invest, 1997: 368–374.doi: 10.1177/104063879700900405
Samui KL., Oloya J, Munyeme M, Skjerve E. " Risk factors for brucellosis in indigenous cattle reared in livestock-wildlife interface areas of Zambia." Prev. Vet Med, 2007: 306–317.doi: 10.1016/j.prevetmed.2007.03.003
Scholz HC., Revilla-Fernández S Al Dahouk S. "Brucella vulpis sp. nov., isolated from mandibular lymph nodes of red foxes (Vulpes vulpes)." Int J Syst Evol Microbiol, 2016: 2090–2098.doi: 10.1099/ijsem.0.000998
Scholz HC.,Gollner Nockler K. "Brucella inopinata sp. nov., isolated from a breast implant infection." Int J Syst Evol Microbiol, 2010: 801–808.doi: 10.1099/ijs.0.011148-0
Scholz HC.,Hubalek Z Sedlacek I. "Brucella microti sp. nov., isolated from the common vole Microtus arvalis." Int J Syst Evol. Microbio, 2008: 375–382.doi: 10.1099/ijs.0.65356-0
Silva J.B., Rangel C.P., Fonseca A.H., Morais E., Vinhote W.M., Lima D.H.S., Silva N.S. & Barbosa J.D. " Serological survey and risk factors for brucellosis in water buffaloes in the state of Pará, Brazil." Trop. Anim. Health Prod., 2014b: :385-389.doi: 10.1007/s11250-013-0501-5
SoleraJ.,Lozano E, Martinez-Alfaro E, Espinosa A, Castillejos ML, Abad L. " Brucellar spondylitis: review of 35 cases and literature survey." Clin Infect Dis, 1999: 1440–49.doi: 10.1086/313524
Sossidou E. "Epizotiological study and model of sheep and goat brucellosis in Greece” Thessis, Aristotle University of Thessaloniki, 1993.
JergefaT., B. Kelay, M. Bekana, S. Teshale, H. Gustafson & H. Kindahl. "Epidemiological study of bovine brucellosis in three agro-ecological areas of central Oromiya, Ethiopia." Rev. sci. tech. Off. int. Epiz, 2009: 933-943.doi: 10.20506/rst.28.3.1939
Tsegayea Y., Mosses Kyuleb, FikreLobago. "Seroprevalence and Risk Factors of Bovine Brucellosis in Arsi Zone, Oromia Regional State, Ethiopia." American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS), 2016: pp 16-25.
Valergakis G., Arsenos G., Oikonomou G., "Biosecurity measures on cattle farms" Journal of the Hellenic Veterinary Medical Society, 59(1), 9-22. doi:10.12681/jhvms.14943
Whatmore AM., Davison N, Cloeckaert A. "Brucella papionis sp. nov., isolated from baboons (Papio spp.)." Int J Syst Evol Microbiol, 2014: 4120–4128.doi: 10.1099/ijs.0.065482-0
Yantzis D., "Brucella eradication program: Course and considerations serological and microbiological tests΄results." Bulletin of the Hellenic Veterinary Medical Society, 1984.doi:10.12681/jhvms.21627
Most read articles by the same author(s)