Determination genetic variation and phylogenetic analysis in Echinococcus granulosus isolated from Iraqi sheep
Περίληψη
Echinococcus granulosus causes Cystic Echinococcosis disease in intermediate hosts, such as sheep, cattle, goats, camels, and horses. Cystic Echinococcosis is an essential parasitic zoonosis that affects humans and animals and is a significant public health and economic problem worldwide. This study aimed to determine the molecular characterization and phylogenetic analysis of Echinococcus granulosus in sheep's livers and lungs. The genetic variation was performed by directly sequencing the mitochondrial DNA (mtDNA) genes coding of the NADH dehydrogenase subunit 1 (ND1). The evidence from molecular studies and DNA sequencing revealed that the Isolates of Echinococcus granulosus in Kirkuk city related to the sheep strain G1 genotype. The alignment analysis showed a concordance between isolates with each other by 99%. When comparing the results with Genbank, it indicated the occurrence of genetic variations between nucleotides represented by eleven codons, seven of which represent transversion mutations, and four are transition mutations.
Λεπτομέρειες άρθρου
- Πώς να δημιουργήσετε Αναφορές
-
Bayraktar, M., Hussein, M., & Hassan, H. (2022). Determination genetic variation and phylogenetic analysis in Echinococcus granulosus isolated from Iraqi sheep. Περιοδικό της Ελληνικής Κτηνιατρικής Εταιρείας, 73(2), 4245–4252. https://doi.org/10.12681/jhvms.28066
- Τεύχος
- Τόμ. 73 Αρ. 2 (2022)
- Ενότητα
- Research Articles
Αυτή η εργασία είναι αδειοδοτημένη υπό το CC Αναφορά Δημιουργού – Μη Εμπορική Χρήση 4.0.
Οι συγγραφείς των άρθρων που δημοσιεύονται στο περιοδικό διατηρούν τα δικαιώματα πνευματικής ιδιοκτησίας επί των άρθρων τους, δίνοντας στο περιοδικό το δικαίωμα της πρώτης δημοσίευσης.
Άρθρα που δημοσιεύονται στο περιοδικό διατίθενται με άδεια Creative Commons 4.0 Non Commercial και σύμφωνα με την άδεια μπορούν να χρησιμοποιούνται ελεύθερα, με αναφορά στο/στη συγγραφέα και στην πρώτη δημοσίευση για μη κερδοσκοπικούς σκοπούς.
Οι συγγραφείς μπορούν να καταθέσουν το άρθρο σε ιδρυματικό ή άλλο αποθετήριο ή/και να το δημοσιεύσουν σε άλλη έκδοση, με υποχρεωτική την αναφορά πρώτης δημοσίευσης στο J Hellenic Vet Med Soc
Οι συγγραφείς ενθαρρύνονται να καταθέσουν σε αποθετήριο ή να δημοσιεύσουν την εργασία τους στο διαδίκτυο πριν ή κατά τη διαδικασία υποβολής και αξιολόγησής της.
Λήψεις
Τα δεδομένα λήψης δεν είναι ακόμη διαθέσιμα.
Αναφορές
Abdulla R G, Mageed S N, Obed C E, Jumaa J A (2020) Molecular characterization
of fertile hydatid cysts from the liver of the sheep and
cows and associated environmental influence factors. Iraqi Journal of
Veterinary Sciences 34:321-27.
Hammad S J, Cavallero S, Milardi G L, Gabrielli S, Al-Nasiri F S (2018)
Molecular genotyping of Echinococcus granulosus in the North of
Iraq. Veterinary parasitology 15:82-87.
Hasan H F, Fadhil M H, Fadhil Z H (2016) Molecular characterization of
Echinococcus granulosus isolated from human and domestic animals
in Kirkuk, Iraq. Animal Research International 13:2544-47.
Alvi M A, Ohiolei J A, Saqib M, Li L, Tayyab M H, Alvi A A, Wu Y-T,
Fu B-Q, Yan H-B, Jia W-Z (2020) Echinococcus granulosus (sensu
stricto) (G1, G3) and E. ortleppi (G5) in Pakistan: phylogeny, genetic
diversity and population structural analysis based on mitochondrial
DNA. Parasites & vectors 13:1-10.
Kadhimand H A A, Al-Mayali H M H (2020) Molecular characterizations
of Echinococcus granulosus isolated from human and sheep in Euphrates
region of Iraq. International Journal of Pharmaceutical Research
:2296-303.
Mahdi Z M S, Al-Hamairy A K, Al-Rubaiey H M (2020) Genotyping of
Echinococcusgranulosus Isolates from Human, Sheep and Cattles
Hydatid Cysts in Some Central Euphrates Provinces, Iraq. Medico
Legal Update 20:570-75.
Saadi A. 2020. Contribution to the evaluation of the control program of
cystic echinococcosis in Morocco. University of Liège, Liege, Belgium
Sharma M, Sehgal R, Fomda B A, Malhotra A, Malla N (2013) Molecular
characterization of Echinococcus granulosus cysts in north Indian patients:
identification of G1, G3, G5 and G6 genotypes. PLoS neglected
tropical diseases 7:1-6.
Fallahizadeh S, Arjmand R, Jelowdar A, Rafiei A, Kazemi F (2019) Determination
of Echinococcus granulosus genotypes in livestock slaughtered
in Shush County, Southwest Iran using PCR-RFLP. Helminthologia
:196-201.
Alsaady H A M, Al-Quzweeni H A N (2019) Molecular study of Echinococcus
Granulosus in Misan Province, south of Iraq. Indian Journal
of Public Health 10:1047.
Lavikainen A, Lehtinen M, Meri T, Hirvelä-Koski V, Meri S (2003) Molecular
genetic characterization of the Fennoscandian cervid strain, a
new genotypic group (G10) of Echinococcus granulosus. Parasitology
:207-15.
Piccoli L, Bazzocchi C, Brunetti E, Mihailescu P, Bandi C, Mastalier B,
Cordos I, Beuran M, Popa L, Meroni V (2013) Molecular characterization
of Echinococcus granulosus in south-eastern Romania: evidence
of G1-G3 and G6-G10 complexes in humans. Clinical Microbiology
and Infection 19:578-82.
Yan B, Liu X, Wu J, Zhao S, Yuan W, Wang B, Wureli H, Tu C, Chen C
(2018) Genetic diversity of Echinococcus granulosus genotype G1
in Xinjiang, Northwest of China. The Korean journal of parasitology
:391-96.
Smyth J (1985) In vitro culture of Echinococcus spp. Proceedings of the
th Int. Congr. Hydatidology. Madrid 84-9.
Sánchez E, Cáceres O, Náquira C, Miranda E, Samudio F, Fernandes O
(2012) Echinococcus granulosus genotypes circulating in alpacas
(Lama pacos) and pigs (Sus scrofa) from an endemic region in Peru.
Memórias do Instituto Oswaldo Cruz 107:275-78.
Wen H, Vuitton L, Tuxun T, Li J, Vuitton D A, Zhang W, Mcmanus D P
(2019) Echinococcosis: advances in the 21st century. Clinical microbiology
reviews 32:1-39.
Kern P, Da Silva A M, Akhan O, Müllhaupt B, Vizcaychipi K, Budke C,
Vuitton D (2017) The echinococcoses: diagnosis, clinical management
and burden of disease. Advances in parasitology 96:259-369.
Nakao M, Lavikainen A, Iwaki T, Haukisalmi V, Konyaev S, Oku Y,
Okamoto M, Ito A (2013) Molecular phylogeny of the genus Taenia
(Cestoda: Taeniidae): proposals for the resurrection of Hydatigera Lamarck,
and the creation of a new genus Versteria. International
Journal for Parasitology 43:427-37.
Hamoo R, Abdulraheem S (2019) Nad1 gene analysis of Echinococcus
granulosus from sheep in Aqrah city, Iraq. Iraqi Journal of Veterinary
Sciences 33:341-45.
Fadhil S, A’aiz N (2016) Genotyping of cystic echinococcosis isolates
from clinical samples of human and domestic animals. Iraqi Journal
of Veterinary Sciences 30:33-39.
Mustafa J Y, Al-Azizz S A, Lazim A R (2019) Molecular study of hydatid
cysts in human, sheep and donkeys at basra city, southern iraq. Biochemical
and Cellular Archives 19:4155-61.
Al-Asadi S A M, Hansh W J, Awad A-H H (2021) Employing NADH Dehydrogenase
Subunit 1 in the Determination of Echinococcus granulosus
Strain in Sheep, Cattle and Human in Thi-Qar Province, Iraq.
Baghdad Science Journal 18:0238-38.
Muhaidi M, Maysam A, Mohammed D (2017) Determination the causative
strain for hydatid cyst in Iraqi cattle by using ND1 gene. Iraqi
Journal of Veterinary Medicine 41:11-16.
Muhaidi M, Ahmed M, Aziz L (2018) Identification of Echinococcus
Granulosus Genotype in Iraqi’s Sheep by Using Nd1 Gene. Journal of
Global Pharma Technology 10:261-66.
Barazesh A, Sarkari B, Sarısu G, Hami M, Mikaeili F, Aydın A, Ekici A,
Ebrahimi S (2019) Comparative genotyping of Echinococcus granulosus
infecting livestock in Turkey and Iran. Turkish Journal of Parasitology
:123-29.
Hajialilo E, Harandi M F, Sharbatkhori M, Mirhendi H, Rostami S (2012)
Genetic characterization of Echinococcus granulosus in camels, cattle
and sheep from the south-east of Iran indicates the presence of the G3
genotype. Journal of helminthology 86:263-70.
Pezeshki A, Akhlaghi L, Sharbatkhori M, Razmjou E, Oormazdi H, Mohebali
M, Meamar A (2013) Genotyping of Echinococcus granulosus
from domestic animals and humans from Ardabil Province, northwest
Iran. Journal of helminthology 87:387-91.
Sharbatkhori M, Tanzifi A, Rostami S, Rostami M, Harandi M F (2016)
Echinococcus granulosus sensu lato genotypes in domestic livestock
and humans in Golestan Province, Iran. Revista do Instituto de Medicina
Tropical de São Paulo 58:1-7.
Ahmed M E, Salim B, Grobusch M P, Aradaib I E (2018) First molecular
characterization of Echinococcus granulosus (sensu stricto) genotype
among cattle in Sudan. BMC veterinary research 14:1-5.
Amer S, Helal I B, Kamau E, Feng Y, Xiao L (2015) Molecular characterization
of Echinococcus granulosus sensu lato from farm animals in
Egypt. PLoS One 10:1-12.
Kim H-J, Yong T-S, Shin M H, Lee K-J, Park G-M, Suvonkulov U, Kovalenko
D, Yu H S (2020) Phylogenetic Characteristics of Echinococcus
granulosus sensu lato in Uzbekistan. The Korean journal of parasitology
:205-10.
Sánchez E, Cáceres O, Náquira C, Garcia D, Patiño G, Silvia H, Volotão
A C, Fernandes O (2010) Molecular characterization of Echinococcus
granulosusfrom Peru by sequencing of the mitochondrial cytochrome
C oxidase subunit 1 gene. Memórias do Instituto Oswaldo
Cruz 105:806-10.
Ohiolei J A, Xia C-Y, Li L, Liu J-Z, Tang W-Q, Wu Y-T, Zhu G-Q, Shi
B, Fu B-Q, Yin H (2019) Genetic variation of Echinococcus spp. in
yaks and sheep in the Tibet Autonomous Region of China based on
mitochondrial DNA. Parasites & vectors 12:1-10.
Larrieu E, Gavidia C M, Lightowlers M W (2019) Control of cystic echinococcosis:
background and prospects. Zoonoses and public health
:889-99.
Mokhtaria K, Fatima B-K, Abboud B, Ammar S S M (2013) Cystic echinococcosis
in small ruminants in Tiaret (Algeria). Global Veterinaria
:753-58.
