NRF2 is a potential biomarker for the evaluation of weaning-related oxidative stress in goat kids

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Δημοσιευμένα: Jan 21, 2023
DOI: https://doi.org/10.12681/jhvms.28455
H Özkan
Hatay Mustafa Kemal University
https://orcid.org/0000-0001-5753-8985
U Kaya
Hatay Mustafa Kemal University
https://orcid.org/0000-0002-4805-0993
İ Karaaslan
Hatay Mustafa Kemal University
https://orcid.org/0000-0002-7485-192X
S Dalkiran
Hatay Mustafa Kemal University
https://orcid.org/0000-0002-5704-5774
B Çamdeviren
Hatay Mustafa Kemal University
https://orcid.org/0000-0003-1508-7869
M Yüksel
Hatay Mustafa Kemal University
https://orcid.org/0000-0001-6634-0559
A Yakan
https://orcid.org/0000-0002-9248-828X
Περίληψη

Weaning stress is crucial threatening factor in animal breeding. The aim of this study was to investigate the effects of weaning to oxidative stress and stress status of goat kids at the molecular levels. In the study, blood samples were collected at the weaning process (7 days before weaning- Pre-W, Day of weaning- Day-W and 7 days after weaning- Post-W) from 24 healthy Damascus goats. Cortisol, MDA (Malondialdehyde), COX-2 (Cyclooxygenase-2), and NRF2 (Nuclear factor erythroid 2-related factor 2) proteins levels were investigated in plasma, while COX-2 and NRF2 genes expression levels were determined from leukocytes. Compared to Pre-W, COX-2 gene was upregulated almost 2-fold in Day-W. On the other hand, NRF2 gene expression levels were tended to upregulation in Day-W, and it was upregulated approximately 2-fold in Post-W. While MDA levels were gradually increased in the weaning process, the levels of COX-2 and NRF2 proteins showed similar changes with gene expression trends. Positive correlations were also found between cortisol and COX-2 and NRF-2 protein levels in plasma. It was thought that significant outputs gained in terms of MDA levels in plasma of kids at the weaning process. For the first time, determination of COX-2 and NRF2 levels both mRNA and protein levels in the weaning process of goat kids were satisfactorily evaluated in this study. Particularly, NRF2 was found to have the potential to be a fundamental biomarker to assess the oxidative status of weaning kids.

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Λήψεις
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Βιογραφικό Συγγραφέα
H Özkan, Hatay Mustafa Kemal University

Department of Genetics

Αναφορές
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