NRF2 is a potential biomarker for the evaluation of weaning-related oxidative stress in goat kids

PDF (English)
Publiée : Ιαν 21, 2023
DOI : https://doi.org/10.12681/jhvms.28455
H Özkan
Hatay Mustafa Kemal University
https://orcid.org/0000-0001-5753-8985
U Kaya
Hatay Mustafa Kemal University
https://orcid.org/0000-0002-4805-0993
İ Karaaslan
Hatay Mustafa Kemal University
https://orcid.org/0000-0002-7485-192X
S Dalkiran
Hatay Mustafa Kemal University
https://orcid.org/0000-0002-5704-5774
B Çamdeviren
Hatay Mustafa Kemal University
https://orcid.org/0000-0003-1508-7869
M Yüksel
Hatay Mustafa Kemal University
https://orcid.org/0000-0001-6634-0559
A Yakan
https://orcid.org/0000-0002-9248-828X
Résumé

Weaning stress is crucial threatening factor in animal breeding. The aim of this study was to investigate the effects of weaning to oxidative stress and stress status of goat kids at the molecular levels. In the study, blood samples were collected at the weaning process (7 days before weaning- Pre-W, Day of weaning- Day-W and 7 days after weaning- Post-W) from 24 healthy Damascus goats. Cortisol, MDA (Malondialdehyde), COX-2 (Cyclooxygenase-2), and NRF2 (Nuclear factor erythroid 2-related factor 2) proteins levels were investigated in plasma, while COX-2 and NRF2 genes expression levels were determined from leukocytes. Compared to Pre-W, COX-2 gene was upregulated almost 2-fold in Day-W. On the other hand, NRF2 gene expression levels were tended to upregulation in Day-W, and it was upregulated approximately 2-fold in Post-W. While MDA levels were gradually increased in the weaning process, the levels of COX-2 and NRF2 proteins showed similar changes with gene expression trends. Positive correlations were also found between cortisol and COX-2 and NRF-2 protein levels in plasma. It was thought that significant outputs gained in terms of MDA levels in plasma of kids at the weaning process. For the first time, determination of COX-2 and NRF2 levels both mRNA and protein levels in the weaning process of goat kids were satisfactorily evaluated in this study. Particularly, NRF2 was found to have the potential to be a fundamental biomarker to assess the oxidative status of weaning kids.

Article Details
  • Rubrique
  • Research Articles
Creative Commons License

Ce travail est disponible sous licence Creative Commons Attribution - Pas d’Utilisation Commerciale 4.0 International.

Authors who publish with this journal agree to the following terms:

· Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Non-Commercial License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

· Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

· Authors are permitted and encouraged to post their work online (preferably in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Téléchargements
Les données relatives au téléchargement ne sont pas encore disponibles.
Biographie de l'auteur
H Özkan, Hatay Mustafa Kemal University

Department of Genetics

Références
Baldwin RL, McLeod KR, Klotz JL, Heitmann RN (2004) Rumen development, intestinal growth and hepatic metabolism in the pre-and postweaning ruminant. J Dairy Sci 87: 55-65.
Barbieri SS, Eligini S, Brambilla M, Tremoli E, Colli S (2003) Reactive oxygen species mediate cyclooxygenase-2 induction during monocyte to macrophage differentiation: critical role of NADPH oxidase. Cardiovascular research 60(1): 187-197.
Bertoni, G., Trevisi, E., Lombardelli, R., & Bionaz, M. (2005) Plasma cortisol variations in dairy cows after some usual or unusual manipulations. Ital J Anim Sci 4(2): 200-202.
Deng, S, Yu K, Jiang W, Li Y, Wang S, Deng Z, Yao Y, Zhang B, Liu G, Liu Y, Lian Z (2017) Over-expression of Toll-like receptor 2 up-regulates heme oxygenase-1 expression and decreases oxidative injury in dairy goats. J Anim Sci Biotechnol 8(1): 1-10.
Esterbauer H, Cheeseman KH (1990). Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. In: Methods in enzymology, 1st ed, Academic Press, USA: pp 407-421.
Galarza EM, Lizarraga RM, Mattioli GA, Parker AJ, Relling AE (2021) Effect of preshipment preconditioning and injectable antioxidant trace elements (Cu, Mn, Se, Zn) and vitamins (A, E) on plasma metabolite and hormone concentrations and growth in weaned beef cattle. Transl Anim Sci 5(1): 1-11.
Gessner DK, Fiesel A, Most E, Dinges J, Wen G, Ringseis R, Eder . (2013. Supplementation of a grape seed and grape marc meal extract decreases activities of the oxidative stress-responsive transcription factors NF-κB and Nrf2 in the duodenal mucosa of pigs. Acta Vet Scand 55(1): 1-10.
Izuddin WI, Loh TC, Samsudin AA, Foo HL, Humam AM, Shazali N (2019) Effects of postbiotic supplementation on growth performance, ruminal fermentation and microbial profile, blood metabolite and GHR, IGF-1 and MCT-1 gene expression in post-weaning lambs. BMC Vet Res 15(1):1-10.
Jasper J, Weary DM (2002) Effects of ad libitum milk intake on dairy calves. J Dairy Sci 85(11): 3054-3058.
Jin X, Wang K, Liu H, Hu F, Zhao F, Liu J (2016) Protection of bovine mammary epithelial cells from hydrogen peroxide-induced oxidative cell damage by resveratrol. Oxid Med Cell Longev 1-15.
Katoh K, Yoshida M, Kobayashi Y, Onodera M, Kogusa K, Obara Y (2005) Responses induced by arginine-vasopressin injection in the plasma concentrations of adrenocorticotropic hormone, cortisol, growth hormone and metabolites around weaning time in goats. J Endocrinol 187(2): 249-256.
Kerasioti E, Stagos D, Tzimi A, Kouretas D (2016) Increase in antioxidant activity by sheep/goat whey protein through nuclear factor-like 2 (Nrf2) is cell type dependent. Food Chem Toxicol 97: 47-56.
Kim MH, Yang JY, Upadhaya SD, Lee HJ, Yun CH, Ha JK (2011) The stress of weaning influences serum levels of acute-phase proteins, iron-binding proteins, inflammatory cytokines, cortisol, and leukocyte subsets in Holstein calves. J Vet Sci 12(2): 151-157.
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method. Methods 25(4): 402-408.
Luo C, Urgard E, Vooder T, Metspalu A (2011) The role of COX-2 and Nrf2/ARE in anti-inflammation and antioxidative stress: Aging and anti-aging. Med Hypotheses 77(2): 174-178.
Magistrelli D, Aufy AA, Pinotti L, Rosi F (2013) Analysis of weaning‐induced stress in Saanen goat kids. J Anim Physiol Anim Nutr 97(4): 732-739.
Memon MA, Wang Y, Xu T, Ma N, Zhang H, Roy AC, Aabdin Z, Shen X (2019) Lipopolysaccharide induces oxidative stress by triggering MAPK and Nrf2 signalling pathways in mammary glands of dairy cows fed a high-concentrate diet. Microb Pathog 128: 268-275.
Modesto P, Peletto S, Pisoni, G, Cremonesi P, Castiglioni B, Colussi S, Caramelli M, Bronzo V, Moroni P, Acutis PL (2013) Evaluation of internal reference genes for quantitative expression analysis by real-time reverse transcription-PCR in somatic cells from goat milk. J Dairy Sci 96(12): 7932-7944.
Ognik K, Patkowski K, Gruszecki T, Kostro K, Cholewińska E (2017) Blood antioxidant potential in growing lambs of synthetic SCP line. Small Rumin Res 149: 73-76.
Poh L, Goh W, Chong ETJ, Lee PC (2016) High integrity total RNA isolation from human peripheral blood that is as competitive to commercialize kits. Res J Pharm Biol Chem Sci 7(4): 1937-1942.
Pol F, Courboulay V, Cotte JP, Martrenchar A, Hay M. Mormède P (2002) Urinary cortisol as an additional tool to assess the welfare of pregnant sows kept in two types of housing. Vet Res 33(1): 13-22.
Redondo E, Franco A, Garcia A, Masot AJ (2010) Changes in concentrations of cortisol and melatonin in plasma, expression of synaptophysin, and ultrastructural properties of pinealocytes in goat kids in situations of stress due to early weaning: the effect of melatonin. N Z Vet J 58(3): 160-167.
Rio DC, Ares M, Hannon GJ, Nilsen TW (2010) Purification of RNA using TRIzol (TRI reagent). Cold Spring Harb Protoc 2010(6): 1-3.
Tao X, Xu Z, Men X (2016) Transient effects of weaning on the health of newly weaning piglets. Czech J Anim Sci 61(2): 82-90.
Ugur F, Atasoglu C, Tolu C, Diken F, Pala A (2007) Effects of different weaning programs on growth of Saanen kids. Anim Sci J 78(3): 281-285.
Yin J, Wu MM, Xiao H, Ren WK, Duan JL, Yang G, Li TJ, Yin YL (2014) Development of an antioxidant system after early weaning in piglets. J Anim Sci 92(2): 612-619.
Yonny ME, García EM, Lopez A, Arroquy JI, Nazareno, MA (2016) Measurement of malondialdehyde as oxidative stress biomarker in goat plasma by HPLC-DAD. Microchem J 129: 281-285.
Yakan A, Özkan H, Çamdeviren B, Kaya U, Karaaslan İ, Dalkiran S (2021) Expression patterns of major genes in fatty acid synthesis, inflammation, oxidative stress pathways from colostrum to milk in Damascus goats. Scientific Reports 11(1):1-10.
Zobel G, Freeman H, Watson T, Cameron C, Sutherland M (2020) Effect of different milk-removal strategies at weaning on feed intake and behavior of goat kids. J Vet Behav 35: 62-68.
Articles les plus lus par le même auteur ou la même autrice